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公开(公告)号：US20240293479A1
公开(公告)日：2024-09-05
申请号：US18593178
申请日：2024-03-01
IPC分类号： A61K35/741 , A61K9/19 , C12N1/20 , C12R1/46
CPC分类号： A61K35/741 , A61K9/19 , C12N1/205 , C12N2500/16 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/74 , C12N2500/84 , C12N2501/998 , C12R2001/46
摘要： Provided is a method of producing a freeze-dried probiotic composition as well as a method of producing a probiotic raw material which involves culturing of a Streptococcus salivarius strain. Furthermore, a corresponding probiotic composition and a corresponding probiotic raw material is provided.
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公开(公告)号：US20240201170A1
公开(公告)日：2024-06-20
申请号：US18555348
申请日：2022-04-18
CPC分类号： G01N33/5011 , C12N5/0693 , G01N33/5082 , C12N2500/50 , C12N2501/727 , C12N2533/50 , C12N2533/80 , C12N2537/10 , G01N2500/10
摘要： The present invention relates to the maintenance and/or culture of tissue slices in vitro or ex vivo. In particular, the present invention relates to a method for the maintenance and/or culture of tissue slices using hydrogels. In one embodiment, the hydrogel is a semi-synthetic hydrogel comprising thiolated hyaluronan with acrylated peptides comprising KGGGPQGIWGQGK (SEQ ID NO: 1) that are degradable by matrix metalloproteinases. In particular, the present invention provides a platform suitable for downstream analysis such as real-time imaging, other real-time study of the tissue in culture and testing of a therapeutic agent or a potential therapeutic agent.
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公开(公告)号：US11926847B2
公开(公告)日：2024-03-12
申请号：US17045471
申请日：2019-04-03
发明人： Raunak Jay Soman , Jay Soman , Motukuri Mahesh
CPC分类号： C12N5/061 , C12N5/0612 , C12N2500/05 , C12N2500/14 , C12N2500/16 , C12N2500/30 , C12N2500/32 , C12N2500/50 , C12N2500/84
摘要： Disclosed is a composition for selective enrichment of progressively motile non-fragmented/intact DNA sperms during swim-up of semen sample comprising combination of electrolytes, a mix of excipients, polyyvinylpyrrolidone, recombinant human serum albumin or bovine serum albumin. A method of selective enrichment of DNA-intact sperms using the compositions of the present invention is also disclosed. The DNA fragmentation index of the swim-up sperms are less than the DNA fragmentation index of the neat samples. The media compositions of the present invention are applicable for enrichment of non-fragmented/intact DNA sperms for therapeutic purposes in the field of assisted reproductive technologies.
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公开(公告)号：US20240010982A1
公开(公告)日：2024-01-11
申请号：US18174853
申请日：2023-02-27
申请人： YiPCELL Inc.
发明人： Ji Hyeon JU , Yoo Jun NAM , Yeri RIM
CPC分类号： C12N5/0655 , A61K35/32 , C12N15/85 , C12N2500/50 , C12N2501/15 , C12N2501/155 , C12N2501/998 , C12N2506/45 , C12N2533/54
摘要： The present invention relates to a method for inducing differentiation, into chondrocytes, of cord blood mononuclear cell-derived induced pluripotent stem cells. In a case where a chondrogenic pellet produced by the method of the present invention is transplanted into a cartilage damage area in vivo, regeneration of cartilage can be effectively exhibited by differentiated chondrocytes. In such a case, an effective cartilage regeneration capacity can be exhibited as compared with a case where chondrocytes produced by differentiation induction with the addition of a recombinant growth factor are transplanted. Thus, the present invention can be usefully used for tissue engineering therapies.
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公开(公告)号：US20230407265A1
公开(公告)日：2023-12-21
申请号：US18320106
申请日：2023-05-18
申请人： CITY OF HOPE
发明人： Keiko OMORI , Fouad KANDEEL , Meirigeng QI
CPC分类号： C12N5/0677 , A61P3/10 , C12N2501/345 , C12N2500/50 , C12N2500/34
摘要： Provided herein, inter alia, are compositions and methods for culturing islet cells.
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公开(公告)号：US20230332100A1
公开(公告)日：2023-10-19
申请号：US18133774
申请日：2023-04-12
申请人： MERCK PATENT GMBH
发明人： Alice ANTONELLO , Tim Hofmann , Almut RAPP
IPC分类号： C12N5/00
CPC分类号： C12N5/00 , C12N2500/50
摘要： The present invention relates to the use of meroxapols, also called “reverse poloxamers”, as cell culture media additives. The meroxapols are suitable for foam reduction as well as for shear stress protection.
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公开(公告)号：US11746360B2
公开(公告)日：2023-09-05
申请号：US16788303
申请日：2020-02-11
申请人： Florian M. Wurm , Maria J. Wurm , Maria de Lourdes Rodrigues , Divor Kiseljak , Cedric Bürki , Cyril Pugin , Guillaume Raussin , Julie Heymoz
发明人： Florian M. Wurm , Maria J. Wurm , Maria de Lourdes Rodrigues , Divor Kiseljak , Cedric Bürki , Cyril Pugin , Guillaume Raussin , Julie Heymoz
CPC分类号： C12N15/87 , C12N5/0601 , C12N5/0602 , C12N2500/50 , C12N2510/04
摘要： The present invention provides robust, streamlined, reproducible and highly efficient eukaryotic cell transfection systems and related methods. The highly-efficient systems and methods of the present invention reduce the number of steps required to transfect cells and reduce, e.g., eliminate, the need for specialized equipment. In particular, the systems and related methods afford the ability for streamlining transfection while retaining and improving robust and reproducible transfection efficiencies, cell viability, and/or protein production. Furthermore, the highly-efficient systems and methods of the present invention for transfecting eukaryotic cells also eliminate the need for any specialized or complicated preparation of exogenous nucleic acid, which makes available high throughput and/or large scale transfection.
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公开(公告)号：US20180214606A1
公开(公告)日：2018-08-02
申请号：US15698172
申请日：2017-09-07
申请人： Organogenesis, Inc.
发明人： Dario C. Eklund
CPC分类号： A61L27/3641 , A61K35/12 , A61K35/33 , A61L27/3633 , A61L27/3804 , A61L27/3813 , A61L27/3839 , A61L27/3891 , C12N5/0656 , C12N5/0698 , C12N2500/25 , C12N2500/32 , C12N2500/36 , C12N2500/38 , C12N2500/40 , C12N2500/50 , C12N2500/90 , C12N2501/11 , C12N2501/39 , C12N2501/392 , C12N2501/395 , C12N2502/094 , C12N2502/1323
摘要： A method for treating an oral condition of a subject by grafting cultured tissue constructs to the oral tissue. The cultured tissue constructs comprise cultured cells and endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. Some tissue constructs of the invention are comprised of multiple cell layers or more than one cell type. The tissue constructs of the invention have morphological features and functions similar to tissues their cells are derived and their strength makes them easily handleable. Preferred cultured tissue constructs of the invention comprise cells derived from human tissue.
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9.再颁专利公开(公告)号：USRE46897E1
公开(公告)日：2018-06-19
申请号：US15203550
申请日：2016-07-06
申请人： Baxalta Incorporated , Baxalta GmbH
发明人： Manfred Reiter , Wolfgang Mundt , Friedrich Dorner
CPC分类号： C12N5/0075 , C12N5/0043 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2500/76 , C12N2531/00
摘要： Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
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公开(公告)号：US09758568B2
公开(公告)日：2017-09-12
申请号：US14942771
申请日：2015-11-16
申请人： Baxalta Incorporated , Baxalta GmbH
IPC分类号： C12N1/00 , C07K14/755 , C12N7/00 , C12N5/00
CPC分类号： C07K14/755 , C12N5/0018 , C12N5/005 , C12N7/00 , C12N2500/05 , C12N2500/10 , C12N2500/20 , C12N2500/24 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2710/24151
摘要： The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.
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