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    Method for distinguishing mesenchymal stem cell using molecular marker and use thereof
    1.
    发明授权
    Method for distinguishing mesenchymal stem cell using molecular marker and use thereof 有权
    使用分子标记鉴别间充质干细胞的方法及其用途

    公开(公告)号：US09234897B2

    公开(公告)日：2016-01-12

    申请号：US11887362

    申请日：2006-03-30

    申请人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

    发明人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

    IPC分类号： C12N5/00 ,  G01N33/68 ,  C12Q1/68 ,  G01N33/569

    CPC分类号： G01N33/6845 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/56966

    摘要： Disclosed is a method for distinguishing a mesenchymal stem cell comprising, using at least one gene selected from the genes having the nucleotide sequences indicated by the accession numbers shown in Table 1 as a distinguish marker, detecting the difference in expression of the distinguish marker between a mesenchymal stem cell and a connective tissue cell to distinguish the mesenchymal stem cell from the connective tissue cell. This method enables to distinguish an undifferentiated mesenchymal stem cell from other connective tissue cell such as fibroblasts, osteoblasts, chondrocytes and adipose cells with good accuracy. A mesenchymal stem cell given by this method or a composition comprising the mesenchymal stem sell can be used as a therapeutic for use in the regenerative medicine.

    摘要翻译： 本发明公开了一种区分间充质干细胞的方法，其特征在于，使用选自具有表1所示的登录号的核苷酸序列的基因的至少一种基因作为区别标记，检测所述区别标记的表达差异 间充质干细胞和结缔组织细胞，以区分间充质干细胞与结缔组织细胞。 该方法能够以良好的精度将未分化的间充质干细胞与其他结缔组织细胞如成纤维细胞，成骨细胞，软骨细胞和脂肪细胞区分开。 通过该方法给予的间充质干细胞或包含间充质茎的组合物可用作再生医学中的治疗剂。

    Culturing method of mesenchymal stem cell
    3.
    发明授权
    Culturing method of mesenchymal stem cell 有权
    间充质干细胞的培养方法

    公开(公告)号：US08039256B2

    公开(公告)日：2011-10-18

    申请号：US12892223

    申请日：2010-09-28

    申请人： Yuhiro Sakai ,  Katsuyuki Yamanaka ,  Mika Takeda ,  Tomohisa Okura ,  Koichiro Tsuji

    发明人： Yuhiro Sakai ,  Katsuyuki Yamanaka ,  Mika Takeda ,  Tomohisa Okura ,  Koichiro Tsuji

    IPC分类号： C12N5/00 ,  C12N5/07 ,  C12N5/10 ,  C12N5/071

    CPC分类号： C12N5/0663 ,  C12N2500/50

    摘要： To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.

    摘要翻译： 为了有效地选择和增殖间充质干细胞而不需要专门的分离装置和复杂的分离操作，通过种植骨髓溶液，脐带血，外周血，滑膜中的至少一种来培养间充质干细胞 和填充在容器中的液体培养基中的羊膜包含水作为主要成分，在37℃下的比重为1.06〜1.10，培养温度为37±2℃。 在容器的顶棚侧表面上，优选使用选自由聚乙烯吡咯烷酮涂覆的二氧化硅细粉末，蔗糖和表氯醇的水溶性共聚物和包含三碘芳环的水溶性化合物中的至少一种来调节比重 。

    DIFFERENTIATION-INDUCING CULTURE MEDIUM ADDITIVE AND USE THEREOF
    4.
    发明申请
    DIFFERENTIATION-INDUCING CULTURE MEDIUM ADDITIVE AND USE THEREOF 审中-公开
    差异诱导培养基添加剂及其用途

    公开(公告)号：US20110212523A1

    公开(公告)日：2011-09-01

    申请号：US13127774

    申请日：2009-10-22

    申请人： Yukio Kato ,  Jin Chang Shao ,  Koichiro Tsuji

    发明人： Yukio Kato ,  Jin Chang Shao ,  Koichiro Tsuji

    IPC分类号： C12N5/077

    CPC分类号： C12N5/0654 ,  C12N2500/25 ,  C12N2500/42 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/39 ,  C12N2501/40 ,  C12N2506/1353

    摘要： Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

    摘要翻译： 本发明提供一种分化诱导培养基添加剂，其用于诱导选自干细胞，牙髓细胞，牙周韧带细胞，胎盘，羊膜和成纤维细胞中的至少一种类型的细胞的骨分化。 无血清条件，以及使用分化诱导培养基添加剂。 用于在无血清条件下诱导干细胞分化的本发明的分化诱导培养基添加剂至少含有至少一种选自EGF，FGF和PDGF的生长因子; 地塞米松 和甘油磷酸酯。 本发明的分化诱导培养基添加剂不需要抗坏血酸2-磷酸酯和ITS，它们通常对骨分化至关重要。 此外，通过添加磷脂可以促进骨分化。

    Method for Distinguishing Mesenchymal Stem Cell Using Molecular Marker and Use Thereof
    6.
    发明申请
    Method for Distinguishing Mesenchymal Stem Cell Using Molecular Marker and Use Thereof 有权
    使用分子标记鉴别间充质干细胞的方法及其用途

    公开(公告)号：US20090232773A1

    公开(公告)日：2009-09-17

    申请号：US11887362

    申请日：2006-03-30

    申请人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

    发明人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

    IPC分类号： A61K35/12 ,  C12Q1/68 ,  C12N5/00 ,  C40B30/04 ,  C40B40/02 ,  A61K31/7088 ,  C07K2/00 ,  C07K16/00 ,  C07H21/04

    CPC分类号： G01N33/6845 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/56966

    摘要： Disclosed is a method for distinguishing a mesenchymal stem cell comprising, using at least one gene selected from the genes having the nucleotide sequences indicated by the accession numbers shown in Table 1 as a distinguish marker, detecting the difference in expression of the distinguish marker between a mesenchymal stem cell and a connective tissue cell to distinguish the mesenchymal stem cell from the connective tissue cell. This method enables to distinguish an undifferentiated mesenchymal stem cell from other connective tissue cell such as fibroblasts, osteoblasts, chondrocytes and adipose cells with good accuracy. A mesenchymal stem cell given by this method or a composition comprising the mesenchymal stem sell can be used as a therapeutic for use in the regenerative medicine.

    摘要翻译： 本发明公开了一种区分间充质干细胞的方法，其特征在于，使用选自具有表1所示的登录号的核苷酸序列的基因的至少一种基因作为区别标记，检测所述区别标记的表达差异 间充质干细胞和结缔组织细胞，以区分间充质干细胞与结缔组织细胞。 该方法能够以良好的精度将未分化的间充质干细胞与其他结缔组织细胞如成纤维细胞，成骨细胞，软骨细胞和脂肪细胞区分开。 通过该方法给予的间充质干细胞或包含间充质茎的组合物可用作再生医学中的治疗剂。

    METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION
    7.
    发明申请
    METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION 审中-公开
    制备组织再生的方法

    公开(公告)号：US20090221076A1

    公开(公告)日：2009-09-03

    申请号：US11817437

    申请日：2006-02-28

    申请人： Yukio Kato ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

    发明人： Yukio Kato ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

    IPC分类号： C12N5/02

    CPC分类号： A61L27/18 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/56 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2501/15 ,  C12N2501/39 ,  C12N2533/40 ,  C08L67/04

    摘要： An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglycolic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000 G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration.

    摘要翻译： 本发明的目的是提供一种用于再生软骨组织的片材的制造方法，其使用包含生物吸收性合成高分子如聚乳酸，聚乙醇酸和乳酸共聚物的常规片状多孔体 酸和乙醇酸。 用于再生软骨组织的片材可以分化软骨细胞或干细胞，而不用像常规方法那样通过加压培养细胞而不培养细胞，并且可以将细胞以高效率积累在支持载体中。 用于再生软骨组织的片材通过以下步骤制备： 在包含生物吸收性合成高分子的片状多孔体上接种与软骨细胞分化的软骨细胞或干细胞; 将多孔体吸入培养液中; 对多孔体施加100〜1000G的加速度一定时间; 并且在不施加加速度的情况下培养多孔体。

    Sheet for guiding regeneration of mesenchymal tissue and production method thereof
    9.
    发明申请
    Sheet for guiding regeneration of mesenchymal tissue and production method thereof 审中-公开
    用于引导间充质组织再生的片材及其制造方法

    公开(公告)号：US20060088569A1

    公开(公告)日：2006-04-27

    申请号：US11256107

    申请日：2005-10-24

    申请人： Toshitsugu Kawata ,  Kaoru Tenjo ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

    发明人： Toshitsugu Kawata ,  Kaoru Tenjo ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

    IPC分类号： A61F13/00

    CPC分类号： A61L27/3804 ,  A61F2/2846 ,  A61F2/3094 ,  A61F2002/30062 ,  A61F2210/0004 ,  A61K35/35 ,  A61K2035/124 ,  A61L27/3633 ,  A61L27/3641 ,  A61L27/3839 ,  A61L31/005 ,  C12N5/0663 ,  C12N2533/40

    摘要： To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

    摘要翻译： 为了提供一种用于引导具有改善的成形性，强度，吸收性和效率的间充质组织再生的片材，该片材通过以下步骤制备：在通过冷冻溶解有有机溶剂的生物可吸收聚合物材料制成的多孔片材的表面中含有培养基 并将其干燥，接种从生物组织取出后生长的间充质细胞，并将间充质细胞分化为间充质组织前体细胞，其中间充质组织前体细胞和细胞外基质粘附在含有培养基的多孔片材的表面上， 并且细胞外基质在分化成间充质组织前体细胞的间充质细胞的过程中分泌。