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公开(公告)号：US08877449B2
公开(公告)日：2014-11-04
申请号：US13141063
申请日：2009-12-18
申请人： Yuichi Ono , Tomoya Nakatani , Yasuko Nakagawa
发明人： Yuichi Ono , Tomoya Nakatani , Yasuko Nakagawa
CPC分类号： C12N5/0678
摘要： The present invention provides markers that can selectively distinguish pancreatic progenitor cells. The present invention also provides methods for distinguishing pancreatic progenitor cells by using the markers as an indicator, and reagents to be used in the methods.The present inventors successfully identified the surface marker Nephrin-like 3 (Neph3) which is specifically expressed in pancreatic progenitor cells, and isolated pancreatic progenitor cells using the marker as an indicator or such. Viable pancreatic progenitor cells can be selected by using Neph3 as an indicator without using translated products and transcripts of any foreign genes. The marker is useful in preparing and identifying pancreatic progenitor cells which are applied to regenerative medicine or such for treatment of pancreatic diseases.
摘要翻译： 本发明提供了可以选择性区分胰腺祖细胞的标志物。 本发明还提供了通过使用标记作为指标来区分胰腺祖细胞的方法，以及用于该方法的试剂。 本发明人成功地鉴定了在胰腺祖细胞中特异性表达的表面标记物Nephrin样3（Neph3），以及使用该标记作为指示剂的分离的胰腺祖细胞。 可以通过使用Neph3作为指标来选择可行的胰腺祖细胞，而不使用翻译的产物和任何外来基因的转录物。 该标记物可用于制备和鉴定适用于再生医学或胰腺疾病治疗的胰腺祖细胞。
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公开(公告)号：US08580523B2
公开(公告)日：2013-11-12
申请号：US13352241
申请日：2012-01-17
申请人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
发明人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
CPC分类号： C12Q1/6881 , C12Q1/6883 , C12Q2600/158 , G01N33/6896 , G01N33/9413 , G01N2800/2835
摘要： The present invention identified Lmx1a genes, which are expressed in dopaminergic neurons at all differentiation stages, from proliferating dopaminergic neuron progenitor cells before cell cycle exit to cells after cell cycle exit. Lmx1a expression in cells can be used as an indicator when selecting cells suitable for transplantation therapy for neurodegenerative diseases such as Parkinson's disease, and is useful as a marker for screening agents involved in the induction of dopaminergic neuron differentiation.
摘要翻译： 本发明鉴定了在细胞周期退出至细胞周期后出现的细胞周期性增殖的多巴胺能神经元祖细胞中，在所有分化阶段的多巴胺能神经元中表达的Lmx1a基因。 当选择适合于帕金森病等神经变性疾病的移植治疗的细胞时，细胞中的Lmx1a表达可用作指示剂，并且可用作参与多巴胺能神经元分化诱导的筛选剂的标记。
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公开(公告)号：US08067161B2
公开(公告)日：2011-11-29
申请号：US12064018
申请日：2006-08-18
CPC分类号： C07K16/18 , C07K14/47 , C12Q1/6881 , C12Q2600/158 , G01N33/5073
摘要： The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of a Nato3 gene, or a complementary sequence thereto, and an antibody against a Nato3 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.
摘要翻译： 本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。 根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可与由Nato3基因的核苷酸序列或互补的多核苷酸组成的多核苷酸杂交 序列，以及针对Nato3蛋白或其部分的抗体用于检测或选择多巴胺能神经元增殖祖细胞。
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4.发明申请公开(公告)号：US20100203505A1
公开(公告)日：2010-08-12
申请号：US12064019
申请日：2006-08-18
CPC分类号： G01N33/56966 , C07K16/18 , C12Q1/6881 , C12Q2600/158
摘要： The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of an Msx1 gene or an Msx2 gene, or a complementary sequence thereto, and an antibody against an Msx1 protein or an Msx2 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.
摘要翻译： 本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。 根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可以与由Msx1基因或Msx2基因的核苷酸序列组成的多核苷酸杂交 ，或其互补序列，以及针对用于检测或选择多巴胺能神经元增殖祖细胞的Msx1蛋白或Msx2蛋白或其一部分的抗体。
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公开(公告)号：US20120021417A1
公开(公告)日：2012-01-26
申请号：US13249190
申请日：2011-09-29
CPC分类号： C07K16/18 , C07K14/47 , C12Q1/6881 , C12Q2600/158 , G01N33/5073
摘要： The present invention is a probe, a primer, and an antibody, for detecting a dopaminergic neuron proliferative progenitor cell. According to the present invention, there is provided a polynucleotide probe and a polynucleotide primer for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell, which can hybridize with a polynucleotide consisting of a nucleotide sequence of a Nato3 gene, or a complementary sequence thereto, and an antibody against a Nato3 protein, or a part thereof for use in the detection or selection of a dopaminergic neuron proliferative progenitor cell.
摘要翻译： 本发明是用于检测多巴胺能神经元增殖祖细胞的探针，引物和抗体。 根据本发明，提供了用于检测或选择多巴胺能神经元增殖祖细胞的多核苷酸探针和多核苷酸引物，其可与由Nato3基因的核苷酸序列或互补的多核苷酸组成的多核苷酸杂交 序列，以及针对Nato3蛋白或其部分的抗体用于检测或选择多巴胺能神经元增殖祖细胞。
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6.发明授权Gene specifically expressed in postmitotic dopaminergic neuron precursor cells 有权基因特异性表达于多巴胺能神经元前体细胞公开(公告)号：US08039224B2
公开(公告)日：2011-10-18
申请号：US12575946
申请日：2009-10-08
申请人： Yasuko Nakagawa , Yuichi Ono , Yoshimasa Sakamoto , Eri Mizuhara , Tomoya Nakatani , Yoshimi Takai
发明人： Yasuko Nakagawa , Yuichi Ono , Yoshimasa Sakamoto , Eri Mizuhara , Tomoya Nakatani , Yoshimi Takai
IPC分类号： G01N33/566
CPC分类号： G01N33/6896 , C07K14/47
摘要： A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.
摘要翻译： 通过本发明获得在细胞周期出口后立即在多巴胺能神经元前体细胞中特异性和瞬时表达的新基因65B13。 65B13的细胞表达可用作选择适合于其安全性，存活率和网络形成能力的细胞的指标，用于神经变性疾病如帕金森病的移植治疗。
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7.发明申请GENE SPECIFICALLY EXPRESSED IN POSTMITOTIC DOPAMINERGIC NEURON PRECURSOR CELLS 有权基因在POSTMITOTIC DOPAMINERGIC神经元前体细胞中明确表达公开(公告)号：US20100203570A1
公开(公告)日：2010-08-12
申请号：US12575946
申请日：2009-10-08
申请人： Yasuko Nakagawa , Yuichi Ono , Yoshimasa Sakamoto , Eri Mizuhara , Tomoya Nakatani , Yoshimi Takai
发明人： Yasuko Nakagawa , Yuichi Ono , Yoshimasa Sakamoto , Eri Mizuhara , Tomoya Nakatani , Yoshimi Takai
IPC分类号： C12N5/0793 , C07H21/04 , C07K7/06 , C07K7/08 , C07K14/435 , C12Q1/02
CPC分类号： G01N33/6896 , C07K14/47
摘要： A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.
摘要翻译： 通过本发明获得在细胞周期出口后立即在多巴胺能神经元前体细胞中特异性和瞬时表达的新基因65B13。 65B13的细胞表达可用作选择适合于其安全性，存活率和网络形成能力的细胞的指标，用于神经变性疾病如帕金森病的移植治疗。
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8.发明申请METHODS FOR IDENTIFYING PURKINJE CELLS USING THE CORL2 GENE AS A TARGET 审中-公开使用CORL2基因作为目标鉴定胰腺细胞的方法公开(公告)号：US20090203046A1
公开(公告)日：2009-08-13
申请号：US11813743
申请日：2006-02-01
申请人： Yuichi Ono , Tomoya Nakatani
发明人： Yuichi Ono , Tomoya Nakatani
CPC分类号： C12Q1/6881 , C07K14/47 , C07K16/18 , C12Q2600/158 , G01N33/56966
摘要： Total RNAs were prepared from the ventral and dorsal regions of embryonic day 12.5 mouse mesencephalon, and a cDNA fragment was obtained by the subtraction method (N-RDA). The full-length cDNA was cloned and the nucleotide sequence was determined. The gene was named Corl2. The result of homology search showed that about 850 residues of Corl2 exhibited homology to the XM_355050 sequence deposited in GenBank; however, the remaining ˜150 residues showed no homology. Thus, Corl2 was demonstrated to be a novel gene. The Corl2 expression in various mouse organs was analyzed by RT-PCR, and the result showed that Corl2 expression was specific to brain. The result of immunostaining day 12.5 embryos and postnatal day 12 brains demonstrated that Corl2 is useful as a Purkinje cell marker at any differentiation stages.
摘要翻译： 从胚胎第12.5天小鼠中脑的腹侧和背部区域制备总RNA，通过减法法（N-RDA）获得cDNA片段。 克隆全长cDNA，测定核苷酸序列。 该基因命名为Corl2。 同源性检索结果表明，Corl2的约850个残基与存在于GenBank中的XM_355050序列具有同源性; 然而，剩余的〜150个残基显示没有同源性。 因此，Corl2被证明是一种新的基因。 通过RT-PCR分析各种小鼠器官中的Corl2表达，结果表明Corl2表达对脑有特异性。 免疫染色第12.5天胚胎和出生后12天脑的结果表明，Corl2在任何分化阶段用作浦肯野细胞标记。
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公开(公告)号：US20070254281A1
公开(公告)日：2007-11-01
申请号：US10580989
申请日：2004-11-26
申请人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
发明人： Yuichi Ono , Yasuko Nakagawa , Tomoya Nakatani
CPC分类号： C12Q1/6881 , C12Q1/6883 , C12Q2600/158 , G01N33/6896 , G01N33/9413 , G01N2800/2835
摘要： The present invention identified Lmx1a genes, which are expressed in dopaminergic neurons at all differentiation stages, from proliferating dopaminergic neuron progenitor cells before cell cycle exit to cells after cell cycle exit. Lmx1a expression in cells can be used as an indicator when selecting cells suitable for transplantation therapy for neurodegenerative diseases such as Parkinson's disease, and is useful as a marker for screening agents involved in the induction of dopaminergic neuron differentiation.
摘要翻译： 本发明鉴定了在细胞周期退出至细胞周期后出现的细胞周期性增殖的多巴胺能神经元祖细胞中，在所有分化阶段的多巴胺能神经元中表达的Lmx1a基因。 当选择适合于帕金森病等神经变性疾病的移植治疗的细胞时，细胞中的Lmx1a表达可用作指示剂，并且可用作参与多巴胺能神经元分化诱导的筛选剂的标记。
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公开(公告)号：US20110256548A1
公开(公告)日：2011-10-20
申请号：US13141063
申请日：2009-12-18
申请人： Yuichi Ono , Tomoya Nakatani , Yasuko Nakagawa
发明人： Yuichi Ono , Tomoya Nakatani , Yasuko Nakagawa
IPC分类号： C12Q1/68 , C12N5/071 , G01N33/567
CPC分类号： C12N5/0678
摘要： The present invention provides markers that can selectively distinguish pancreatic progenitor cells. The present invention also provides methods for distinguishing pancreatic progenitor cells by using the markers as an indicator, and reagents to be used in the methods.The present inventors successfully identified the surface marker Nephrin-like 3 (Neph3) which is specifically expressed in pancreatic progenitor cells, and isolated pancreatic progenitor cells using the marker as an indicator or such. Viable pancreatic progenitor cells can be selected by using Neph3 as an indicator without using translated products and transcripts of any foreign genes. The marker is useful in preparing and identifying pancreatic progenitor cells which are applied to regenerative medicine or such for treatment of pancreatic diseases.
摘要翻译： 本发明提供了可以选择性区分胰腺祖细胞的标志物。 本发明还提供了通过使用标记作为指标来区分胰腺祖细胞的方法，以及用于该方法的试剂。 本发明人成功地鉴定了在胰腺祖细胞中特异性表达的表面标记物Nephrin样3（Neph3），以及使用该标记作为指示剂的分离的胰腺祖细胞。 可以通过使用Neph3作为指标来选择可行的胰腺祖细胞，而不使用翻译的产物和任何外来基因的转录物。 该标记物可用于制备和鉴定适用于再生医学或胰腺疾病治疗的胰腺祖细胞。
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