公开(公告)号：US6067158A

公开(公告)日：2000-05-23

申请号：US67296

申请日：1998-04-27

申请人： Yuji Itose ,  Kayo Hatanaka ,  Tomohiro Sakurai

发明人： Yuji Itose ,  Kayo Hatanaka ,  Tomohiro Sakurai

IPC分类号： G01N33/48 ,  G01N15/14 ,  G01N33/49 ,  G01N21/00

CPC分类号： G01N1/38 ,  G01N15/1459 ,  G01N2015/1497

摘要： A method for detecting abnormality in the morphology of erythrocytes, which comprises:(1) mixing a sample containing erythrocytes with a sphere-forming reagent;(2) introducing the sample treated in the preceding step into a detector area of a flow cytometer;(3) measuring at least one scattered light at an angle reflecting the morphology of erythrocyte among the scattered lights emitted from the sample to obtain cell distribution data; and(4) comparing the cell distribution data with cell distribution data on normal erythrocytes that were determined beforehand.

摘要翻译： 一种检测红细胞形态异常的方法，包括：（1）将含有红细胞的样品与球形试剂混合; （2）将前述步骤处理的样品导入流式细胞仪的检测器区域; （3）从样品发射的散射光中以反映红细胞形态的角度测量至少一个散射光，以获得细胞分布数据; 和（4）将细胞分布数据与预先确定的正常红细胞的细胞分布数据进行比较。

公开(公告)号：US5891731A

公开(公告)日：1999-04-06

申请号：US843260

申请日：1997-04-14

申请人： Yasumasa Akai ,  Yuji Itose ,  Kayo Hatanaka ,  Takashi Sakata

发明人： Yasumasa Akai ,  Yuji Itose ,  Kayo Hatanaka ,  Takashi Sakata

IPC分类号： G01N15/14 ,  G01N33/50 ,  G01N31/00 ,  G01N33/48

CPC分类号： G01N33/5094 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

摘要： A reagent for measuring reticulocytes which has at least one dye which specifically stains reticulocytes and at least one dye which specifically stains leukocytes.

摘要翻译： 用于测量网织红细胞的试剂，其具有至少一种特异性染色网织红细胞的染料和至少一种特异性染色白细胞的染料。

公开(公告)号：US5958776A

公开(公告)日：1999-09-28

申请号：US972103

申请日：1997-11-17

申请人： Takashi Sakata ,  Toshihiro Mizukami ,  Kayo Hatanaka

发明人： Takashi Sakata ,  Toshihiro Mizukami ,  Kayo Hatanaka

IPC分类号： G01N33/48 ,  G01N15/14 ,  G01N33/49 ,  G01N33/50 ,  G01N31/00

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

摘要： A hematological sample is treated with a hemolytic agent that maintains immature leukocytes in a viable state and damages other leukocytes. The damaged leukocytes are stained with a fluorochrome which can stain damaged cells. At least one kind of scattered light and at least one kind of fluorescence of the treated and stained blood cells are measured to classify and count leukocytes based on the intensities of the scattered light and the fluorescence. Highly precise measurement of immature leukocytes and simultaneous classification and counting of normal leukocytes are achieved.

摘要翻译： 用溶血剂处理血液样品，其将未成熟白细胞维持在可行状态并损伤其他白细胞。 损伤的白细胞用可染色受损细胞的荧光染料染色。 根据散射光和荧光的强度，测量处理和染色的血细胞中的至少一种散射光和至少一种荧光，以对白细胞进行分类和计数。 实现了对未成熟白细胞的高精度测量，并实现了正常白细胞的同时分类和计数。