公开(公告)号：US08258369B2

公开(公告)日：2012-09-04

申请号：US12224284

申请日：2006-02-24

申请人： Yuko Wakamatsu ,  Kenjiro Ozato ,  Norihiko Ozato, legal representative

发明人： Yuko Wakamatsu ,  Kenjiro Ozato

IPC分类号： C12N15/00 ,  A01K67/027

CPC分类号： A01K67/0273 ,  A01K2227/40 ,  C12N2517/04

摘要： An object of the invention is to effectively prepare a fish embryo with a correct chromosomal ploidy by nuclear transplantation in which an exogenous fish nucleus is transplanted in a cytoplasmic recipient. For this object, the invention comprises a step of preparing a fish embryo by transplanting a fish cell nucleus to an unfertilized egg. The step of preparing a fish embryo comprises a step of imposing physical and/or chemical stress to the unfertilized egg after activation. By imposing such stress, the stage of haplosis in a female nucleus which happens at the early stage of a series of developmental steps occurring in an unfertilized egg is suppressed and the correct ploidy of an obtained embryo is at least secured.

摘要翻译： 本发明的目的是通过核移植有效地制备具有正确染色体倍性的鱼胚，其中将外源鱼核移植到细胞质受体中。 为此，本发明包括通过将鱼细胞核移植到未受精卵而制备鱼胚的步骤。 制备鱼胚的步骤包括在活化后对未受精卵施加物理和/或化学应力的步骤。 通过施加这种压力，抑制发生在未受精卵中的一系列发育步骤的早期发生在雌性核中的单倍体的阶段，并且至少确保获得的胚胎的正确倍性。

公开(公告)号：US06737559B2

公开(公告)日：2004-05-18

申请号：US09814047

申请日：2001-03-22

申请人： Yuko Wakamatsu ,  Kenjiro Ozato ,  Minoru Tanaka ,  Masato Kinoshita

发明人： Yuko Wakamatsu ,  Kenjiro Ozato ,  Minoru Tanaka ,  Masato Kinoshita

IPC分类号： A01K67027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/03 ,  C07K14/43595 ,  C12N15/8509

摘要： This invention relates to a see-through medaka deficient in iridophores, melanophores, xanthophores and leucophores; a see-through medaka deficient in iridophores, melanophores and xanthophores, and whose sex can be identified by the presence or absence of leucophores and/or a DNA marker; and a see-through medaka wherein a specific organ is allowed to produce luminescence by introducing a hybrid gene being a fusion of a promoter of a gene expressing specifically in that organ and a coding region of a gene encoding a fluorescent protein.

摘要翻译： 本发明涉及虹吸孔，黑素素，黄嘌呤和亮氨酸的透明中间缺陷; 一个透明的中草药缺乏在虹膜，黑素和xanthophores，其性别可以通过存在或不存在的亮氨酸和/或DNA标记识别; 和透明中间药物，其中通过引入作为在该器官中特异表达的基因的启动子和编码荧光蛋白的基因的编码区的融合物的杂交基因，允许特定器官产生发光。

公开(公告)号：US20090255005A1

公开(公告)日：2009-10-08

申请号：US12224284

申请日：2006-02-24

申请人： Yuko Wakamatsu ,  Kenjiro Ozato ,  Norihiko Ozato

发明人： Yuko Wakamatsu ,  Kenjiro Ozato ,  Norihiko Ozato

IPC分类号： A01K67/027 ,  C12N15/87 ,  C12N5/00

CPC分类号： A01K67/0273 ,  A01K2227/40 ,  C12N2517/04

摘要： An object of the invention is to effectively prepare a fish embryo with a correct chromosomal ploidy by nuclear transplantation in which an exogenous fish nucleus is transplanted in a cytoplasmic recipient. For this object, the invention comprises a step of preparing a fish embryo by transplanting a fish cell nucleus to an unfertilized egg. The step of preparing a fish embryo comprises a step of imposing physical and/or chemical stress to the unfertilized egg after activation. By imposing such stress, the stage of haplosis in a female nucleus which happens at the early stage of a series of developmental steps occurring in an unfertilized egg is suppressed and the correct ploidy of an obtained embryo is at least secured.

摘要翻译： 本发明的目的是通过核移植有效地制备具有正确染色体倍性的鱼胚，其中将外源鱼核移植到细胞质受体中。 为此，本发明包括通过将鱼细胞核移植到未受精卵而制备鱼胚的步骤。 制备鱼胚的步骤包括在活化后对未受精卵施加物理和/或化学应力的步骤。 通过施加这种压力，抑制发生在未受精卵中的一系列发育步骤的早期发生在雌性核中的单倍体的阶段，并且至少确保获得的胚胎的正确倍性。

公开(公告)号：US20100286049A1

公开(公告)日：2010-11-11

申请号：US12227528

申请日：2006-05-31

申请人： Yuko Wakamatsu ,  Hisashi Hashimoto

发明人： Yuko Wakamatsu ,  Hisashi Hashimoto

IPC分类号： A61K38/17 ,  C12Q1/68 ,  G01N33/53 ,  C12N5/10 ,  C12N15/63 ,  A61K31/7088 ,  C07K14/425 ,  C07K16/18 ,  C07H21/04 ,  A61P13/12

CPC分类号： C07K14/461

摘要： A major gene of a pc mutant of a Japanese Medaka fish (Oryzias latipes) has been identified. Based on this finding, use of the gene is provided. A gene responsible for polycystic kidney in a Japanese Medaka fish is identified in the chromosomal region of the fish by performing a positional cloning on a pc mutant of the fish. The pc gene is a transcription factor having five C2H2-type zind finger regions, and is considered to be a gene homologous to human Gli-similar 3 (Glis3) gene since it shows a high homology in the zinc finger regions.

摘要翻译： 已经确定了日本楝树鱼（Oryzias latipes）的pc突变体的主要基因。 基于这一发现，提供了该基因的使用。 通过在鱼的pc突变体上进行位置克隆，在鱼的染色体区域中鉴定了在日本的Medaka鱼中负责多囊肾的基因。 pc基因是具有5个C2H2型指环区的转录因子，被认为是与人Gli相似3（Glis3）基因同源的基因，因为它在锌指区域中显示高同源性。