公开(公告)号：US07355702B2

公开(公告)日：2008-04-08

申请号：US11153635

申请日：2005-06-15

申请人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

发明人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

IPC分类号： G01J3/44

CPC分类号： G01N21/6458 ,  G02B21/006 ,  G02B21/008 ,  G02B21/32 ,  G02B21/367

摘要： A confocal observation system, comprising, an image acquisition unit for acquiring optical cross sectional images of a three-dimensional specimen, a three-dimensional image construction unit for constructing a three-dimensional image from the optical cross sectional images acquired by the image acquisition unit, a specification unit for specifying a desired three-dimensional region in the three-dimensional image constructed by the three-dimensional image construction unit, and a region acquisition unit for acquiring a cross sectional region to be irradiated with excitation light or stimulation light based on the three-dimensional region specified by the specification unit, wherein the excitation light or the stimulation light irradiates a region in the three-dimensional specimen corresponding to the cross sectional region acquired by the region acquisition unit.

摘要翻译： 一种共聚焦观测系统，包括：用于获取三维样本的光学截面图像的图像获取单元，用于从由图像获取单元获取的光学横截面图像构建三维图像的三维图像构造单元 ，用于指定由三维图像构造单元构成的三维图像中的所需三维区域的指定单元，以及区域获取单元，用于获取基于以下的激励光或刺激光照射的横截面区域： 由所述指定单元指定的三维区域，其中所述激发光或所述刺激光照射所述三维样本中与所述区域获取单元获取的横截面区域相对应的区域。

公开(公告)号：US20050280818A1

公开(公告)日：2005-12-22

申请号：US11153635

申请日：2005-06-15

申请人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

发明人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

IPC分类号： G01J3/30 ,  G01N21/64 ,  G02B21/00 ,  G02B21/36

CPC分类号： G01N21/6458 ,  G02B21/006 ,  G02B21/008 ,  G02B21/32 ,  G02B21/367

摘要： A confocal observation system, comprising, an image acquisition unit for acquiring optical cross sectional images of a three-dimensional specimen, a three-dimensional image construction unit for constructing a three-dimensional image from the optical cross sectional images acquired by the image acquisition unit, a specification unit for specifying a desired three-dimensional region in the three-dimensional image constructed by the three-dimensional image construction unit, and a region acquisition unit for acquiring a cross sectional region to be irradiated with excitation light or stimulation light based on the three-dimensional region specified by the specification unit, wherein the excitation light or the stimulation light irradiates a region in the three-dimensional specimen corresponding to the cross sectional region acquired by the region acquisition unit.

摘要翻译： 一种共聚焦观测系统，包括：用于获取三维样本的光学截面图像的图像获取单元，用于从由图像获取单元获取的光学横截面图像构建三维图像的三维图像构造单元 ，用于指定由三维图像构造单元构成的三维图像中的所需三维区域的指定单元，以及区域获取单元，用于获取基于以下的激励光或刺激光照射的横截面区域： 由所述指定单元指定的三维区域，其中所述激发光或所述刺激光照射所述三维样本中与所述区域获取单元获取的横截面区域相对应的区域。

公开(公告)号：US20070177142A1

公开(公告)日：2007-08-02

申请号：US11726885

申请日：2007-03-23

申请人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

发明人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6456

摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

摘要翻译： 在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。 用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。

公开(公告)号：US07215426B2

公开(公告)日：2007-05-08

申请号：US11213906

申请日：2005-08-30

申请人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

发明人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6456

摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

公开(公告)号：US20060103922A1

公开(公告)日：2006-05-18

申请号：US11265121

申请日：2005-11-03

申请人： Kei Tsuyuki

发明人： Kei Tsuyuki

IPC分类号： G02B21/00

CPC分类号： G02B7/08 ,  G02B21/242

摘要： A specimen and an objective lens can be quickly brought close together or separated without an operator being concerned about the positional relationship between the specimen and the objective lens, and once the correct positional relationship is established, the focal position can be finely adjusted. The invention includes a first focusing mechanism for moving the objective lens in an optical axis direction relative to the specimen so as to approach the specimen or withdraw therefrom; a second focusing mechanism for displacing an internal optical system in the optical axis direction, and a switching device for switching between operating the first focusing mechanism and operating the second focusing mechanism when the objective lens is disposed at a predetermined switching position.

摘要翻译： 样本和物镜可以快速地靠近在一起或分离，而不需要操作者关心样本和物镜之间的位置关系，并且一旦建立了正确的位置关系，就可以微调焦点位置。 本发明包括：第一聚焦机构，用于相对于样本沿光轴方向移动物镜，以便接近样本或从样品中取出; 用于使内部光学系统沿光轴方向移位的第二聚焦机构，以及当物镜设置在预定切换位置时，​​在操作第一聚焦机构和操作第二聚焦机构之间进行切换的切换装置。

公开(公告)号：US20060050276A1

公开(公告)日：2006-03-09

申请号：US11213906

申请日：2005-08-30

申请人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

发明人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6456

摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

摘要翻译： 在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。 用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。

公开(公告)号：US20070028918A1

公开(公告)日：2007-02-08

申请号：US11498474

申请日：2006-08-02

申请人： Kei Tsuyuki ,  Yoshihisa Tanikawa ,  Yoshihiro Kawano

发明人： Kei Tsuyuki ,  Yoshihisa Tanikawa ,  Yoshihiro Kawano

IPC分类号： A61M15/00

CPC分类号： A01K1/031 ,  A22B3/00 ,  A22B3/005 ,  A61D7/04

摘要： The present invention prevents the leakage of anesthetic gas, and enables in vivo observation of a specimen such as small laboratory animals over a long period of time. An anesthetizing chamber comprises a chamber main body for housing a specimen such as a laboratory animal; an anesthetic gas supply part for supplying anesthetic gas to the chamber main body; and an anesthetic gas recovery part for recovering the anesthetic gas that is supplied into the chamber main body from the anesthetic gas supply part; wherein a transparent window is provided to at least the chamber main body.

摘要翻译： 本发明防止了麻醉气体的泄漏，并且能够长时间地在小型实验动物体内观察样本。 麻醉室包括用于容纳诸如实验动物的样本的室主体; 用于向室主体供给麻醉气体的麻醉气体供给部; 以及麻醉气体回收部，其用于回收从所述麻醉气体供给部供给到所述室主体的麻醉气体; 其中，透明窗口至少设置在所述室主体上。

公开(公告)号：US07130117B2

公开(公告)日：2006-10-31

申请号：US11265121

申请日：2005-11-03

申请人： Kei Tsuyuki

发明人： Kei Tsuyuki

IPC分类号： G02B21/00 ,  G02B21/26

CPC分类号： G02B7/08 ,  G02B21/242

摘要： A specimen and an objective lens can be quickly brought close together or separated without an operator being concerned about the positional relationship between the specimen and the objective lens, and once the correct positional relationship is established, the focal position can be finely adjusted. The invention includes a first focusing mechanism for moving the objective lens in an optical axis direction relative to the specimen so as to approach the specimen or withdraw therefrom; a second focusing mechanism for displacing an internal optical system in the optical axis direction, and a switching device for switching between operating the first focusing mechanism and operating the second focusing mechanism when the objective lens is disposed at a predetermined switching position.

公开(公告)号：US20080116395A1

公开(公告)日：2008-05-22

申请号：US12005893

申请日：2007-12-27

申请人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

发明人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

IPC分类号： G01N21/00

CPC分类号： G01N21/6458 ,  G01N21/6456

摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

摘要翻译： 在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。 用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。

公开(公告)号：US07339667B2

公开(公告)日：2008-03-04

申请号：US11726885

申请日：2007-03-23

申请人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

发明人： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6456

摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

摘要翻译： 在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。 用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。