公开(公告)号：US20050280818A1

公开(公告)日：2005-12-22

申请号：US11153635

申请日：2005-06-15

申请人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

发明人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

IPC分类号： G01J3/30 ,  G01N21/64 ,  G02B21/00 ,  G02B21/36

CPC分类号： G01N21/6458 ,  G02B21/006 ,  G02B21/008 ,  G02B21/32 ,  G02B21/367

摘要： A confocal observation system, comprising, an image acquisition unit for acquiring optical cross sectional images of a three-dimensional specimen, a three-dimensional image construction unit for constructing a three-dimensional image from the optical cross sectional images acquired by the image acquisition unit, a specification unit for specifying a desired three-dimensional region in the three-dimensional image constructed by the three-dimensional image construction unit, and a region acquisition unit for acquiring a cross sectional region to be irradiated with excitation light or stimulation light based on the three-dimensional region specified by the specification unit, wherein the excitation light or the stimulation light irradiates a region in the three-dimensional specimen corresponding to the cross sectional region acquired by the region acquisition unit.

摘要翻译： 一种共聚焦观测系统，包括：用于获取三维样本的光学截面图像的图像获取单元，用于从由图像获取单元获取的光学横截面图像构建三维图像的三维图像构造单元 ，用于指定由三维图像构造单元构成的三维图像中的所需三维区域的指定单元，以及区域获取单元，用于获取基于以下的激励光或刺激光照射的横截面区域： 由所述指定单元指定的三维区域，其中所述激发光或所述刺激光照射所述三维样本中与所述区域获取单元获取的横截面区域相对应的区域。

公开(公告)号：US07355702B2

公开(公告)日：2008-04-08

申请号：US11153635

申请日：2005-06-15

申请人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

发明人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

IPC分类号： G01J3/44

CPC分类号： G01N21/6458 ,  G02B21/006 ,  G02B21/008 ,  G02B21/32 ,  G02B21/367

摘要： A confocal observation system, comprising, an image acquisition unit for acquiring optical cross sectional images of a three-dimensional specimen, a three-dimensional image construction unit for constructing a three-dimensional image from the optical cross sectional images acquired by the image acquisition unit, a specification unit for specifying a desired three-dimensional region in the three-dimensional image constructed by the three-dimensional image construction unit, and a region acquisition unit for acquiring a cross sectional region to be irradiated with excitation light or stimulation light based on the three-dimensional region specified by the specification unit, wherein the excitation light or the stimulation light irradiates a region in the three-dimensional specimen corresponding to the cross sectional region acquired by the region acquisition unit.

摘要翻译： 一种共聚焦观测系统，包括：用于获取三维样本的光学截面图像的图像获取单元，用于从由图像获取单元获取的光学横截面图像构建三维图像的三维图像构造单元 ，用于指定由三维图像构造单元构成的三维图像中的所需三维区域的指定单元，以及区域获取单元，用于获取基于以下的激励光或刺激光照射的横截面区域： 由所述指定单元指定的三维区域，其中所述激发光或所述刺激光照射所述三维样本中与所述区域获取单元获取的横截面区域相对应的区域。

公开(公告)号：US20060126170A1

公开(公告)日：2006-06-15

申请号：US11299210

申请日：2005-12-08

申请人： Yusuke Yamashita ,  Tatsuo Nakata ,  Shingo Suzuki

发明人： Yusuke Yamashita ,  Tatsuo Nakata ,  Shingo Suzuki

IPC分类号： G02B21/34

CPC分类号： G01N21/6458 ,  G02B21/16

摘要： Light emitted from a light source is condensed and a sample is irradiated by the condensed beam by an optical system. The light emitted from the sample because of the irradiation is incident on a light detection unit via a wavelength selective optical device having different transmittances depending on wavelengths, a dispersive unit for spectrally resolving light, a wavelength range selection unit for selecting light having a wavelength in a prescribed wavelength range among the light resolved spectrally. A correction unit adjusts a light intensity signal output from the light detection unit based on at least one of a wavelength characteristics of the wavelength selective optical device and the light detection unit respectively.

摘要翻译： 从光源发射的光被冷凝，并且样品被光学系统的聚光束照射。 由于照射而从样品发射的光通过取决于波长的透射率不同的波长选择光学器件入射到光检测单元上，用于光谱分解光的分散单元，用于选择具有波长的光的波长范围选择单元 在光谱中解析光的规定波长范围。 校正单元分别基于波长选择光学器件和光检测单元的波长特性中的至少一个来调整从光检测单元输出的光强度信号。

公开(公告)号：US07696996B2

公开(公告)日：2010-04-13

申请号：US11294689

申请日：2005-12-05

申请人： Toshiyuki Hattori ,  Mitsuhiro Nakano ,  Yusuke Yamashita ,  Hiroshi Hirayama ,  Tatsuo Nakata

发明人： Toshiyuki Hattori ,  Mitsuhiro Nakano ,  Yusuke Yamashita ,  Hiroshi Hirayama ,  Tatsuo Nakata

IPC分类号： G06T11/20

CPC分类号： G02B21/002 ,  G02B21/365

摘要： A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.

摘要翻译： 提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。 控制项目和时间线分别沿垂直轴和水平轴显示。 激光扫描显微镜包括图形用户界面，被配置为沿着时间线设置控制项的控制值;以及控制单元，被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息 通过图形用户界面。

公开(公告)号：US20060129353A1

公开(公告)日：2006-06-15

申请号：US11294689

申请日：2005-12-05

申请人： Toshiyuki Hattori ,  Mitsuhiro Nakano ,  Yusuke Yamashita ,  Hiroshi Hirayama ,  Tatsuo Nakata

发明人： Toshiyuki Hattori ,  Mitsuhiro Nakano ,  Yusuke Yamashita ,  Hiroshi Hirayama ,  Tatsuo Nakata

IPC分类号： G21C17/00

CPC分类号： G02B21/002 ,  G02B21/365

摘要： A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.

摘要翻译： 提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。 控制项目和时间线分别沿垂直轴和水平轴显示。 激光扫描显微镜包括图形用户界面，被配置为沿着时间线设置控制项的控制值;以及控制单元，被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息 通过图形用户界面。

公开(公告)号：US08699128B2

公开(公告)日：2014-04-15

申请号：US12899774

申请日：2010-10-07

申请人： Yusuke Yamashita ,  Tatsuo Nakata

发明人： Yusuke Yamashita ,  Tatsuo Nakata

IPC分类号： G02B21/00

CPC分类号： G02B21/0076 ,  G02B21/0088 ,  G02B21/28 ,  G02B21/30

摘要： A laser scanning microscope includes a culture vessel that accommodates a specimen and is capable of maintaining an interior temperature and humidity thereof, and an optical system space adjacent and optically connected to the culture vessel. The optical system space includes a scanner that two-dimensionally scans ultrashort pulsed laser light across the specimen; an objective lens that focuses the scanned ultrashort pulsed laser light on the specimen and collects light coming from the specimen; a dichroic mirror, disposed between the scanner and the objective lens, that splits off the light coming from the specimen from the laser light; a photodetector that detects the split-off light coming from the specimen; and an outer cover, provided so as to surround the optical system space, that blocks light coming from outside the optical system space.

摘要翻译： 激光扫描显微镜包括容纳样本并能够保持其内部温度和湿度的培养容器以及与培养容器相邻并光学连接的光学系统空间。 光学系统空间包括扫描仪，其跨越样本二维扫描超短脉冲激光; 将扫描的超短脉冲激光聚焦在样品上并收集来自样品的光的物镜; 设置在扫描仪和物镜之间的分色镜，其从激光分离出来自样品的光; 检测来自所述试样的分离光的光检测器; 以及设置为围绕光学系统空间的外盖，其阻挡来自光学系统空间外部的光。

公开(公告)号：US20110089339A1

公开(公告)日：2011-04-21

申请号：US12899774

申请日：2010-10-07

申请人： Yusuke Yamashita ,  Tatsuo Nakata

发明人： Yusuke Yamashita ,  Tatsuo Nakata

IPC分类号： G01J1/58

CPC分类号： G02B21/0076 ,  G02B21/0088 ,  G02B21/28 ,  G02B21/30

摘要： Provided is a laser scanning microscope that allows stable examination by eliminating the influence of ambient light while inhibiting damage to a specimen and fading of fluorescence. Employed is a laser scanning microscope 1 including a culture vessel 6 accommodating a specimen A and capable of maintaining an interior temperature and humidity thereof and an optical system space 5 adjacent to the culture vessel 6 and optically connected to the culture vessel 6. The optical system space 5 includes a scanner 22 that two-dimensionally scans ultrashort pulsed laser light across the specimen A; an objective lens 15 that focuses the scanned ultrashort pulsed laser light on the specimen A and collects light coming from the specimen A; a dichroic mirror 53, disposed between the scanner 22 and the objective lens 15, that splits off the light coming from the specimen A from the laser light; a photodetector 55 that detects the split-off light coming from the specimen A; and a outer cover 2, provided so as to surround the optical system space 5, that blocks light coming from outside the optical system space 5.

摘要翻译： 提供了一种激光扫描显微镜，通过消除环境光的影响，同时抑制样品的损伤和荧光褪色，从而进行稳定的检查。 使用的是激光扫描显微镜1，其包括容纳样本A并能够保持其内部温度和湿度的培养容器6和与培养容器6相邻并光学连接到培养容器6的光学系统空间5。 空间5包括扫描仪22，扫描仪22将超短脉冲激光二维扫描穿过样本A; 物镜15，其将扫描的超短脉冲激光聚焦在样本A上并收集来自样本A的光; 设置在扫描仪22和物镜15之间的分色镜53，其从激光分离出来自检体A的光; 检测来自检体A的分离光的光检测器55; 以及设置为围绕光学系统空间5设置的外盖2，其遮挡来自光学系统空间5的外部的光。

公开(公告)号：US20110102888A1

公开(公告)日：2011-05-05

申请号：US12907196

申请日：2010-10-19

申请人： Susumu Honda ,  Tatsuo Nakata

发明人： Susumu Honda ,  Tatsuo Nakata

IPC分类号： G02B21/08 ,  G02B21/06

CPC分类号： G02B21/088 ,  G02B21/06 ,  G02B21/16 ,  G02B21/32

摘要： Provided is a microscope that allows irradiation with uniform illumination light without decreasing the amount of light. Employed is a microscope 1 including an incoherent light source 31 that emits incoherent light I; an optical fiber 35 on which the incoherent light I is incident and which guides the incident incoherent light I by repeated total reflection; a DMD 37 having an array of movable micromirrors each reflecting or transmitting the guided incoherent light I; an objective lens 18 that irradiates a specimen 19 with the incoherent light I reflected or transmitted by the DMD 37 and that collects fluorescence F coming from the specimen 19; a dichroic mirror 17 that splits off the collected fluorescence F coming from the specimen 19 from the incoherent light I; and a CCD camera 13 that is disposed at a position conjugate to the position of the DMD 37 and that detects the fluorescence F coming from the specimen 19 and split off by the dichroic mirror 17.

摘要翻译： 本发明提供一种能够在不减少光量的情况下照射均匀的照明光的显微镜。 使用的显微镜​​1包括发出非相干光I的非相干光源31; 其中非相干光I入射的光纤35并且通过重复的全反射来引导入射的非相干光I; 具有可移动微镜阵列的DMD37，每个反射或透射被引导的非相干光I; 对由样品19照射由DMD37反射或透射的非相干光I并收集来自样品19的荧光F的物镜18; 分离反射镜17，其分离来自样品19的不相干光I的所收集的荧光F; 以及CCD摄像机13，其设置在与DMD37的位置共轭的位置，并且检测来自检体19的荧光F，并由分色镜17分离。

公开(公告)号：US07483131B2

公开(公告)日：2009-01-27

申请号：US11799196

申请日：2007-05-01

申请人： Nobuhiro Takamizawa ,  Tatsuo Nakata

发明人： Nobuhiro Takamizawa ,  Tatsuo Nakata

IPC分类号： G01J3/30

CPC分类号： G02B21/0076 ,  G02B21/0032 ,  G02B21/006

摘要： An observation laser beam for observing a specimen and a manipulation laser beam for manipulating the specimen are multiplexed; the multiplexed beams irradiate the specimen, which is mounted on a stage, via an objective lens; and fluorescence emitted from inside the specimen in the observation optical axis direction is detected. In a preparation mode, a focal-position adjusting unit is controlled so that the focal position of the observation laser beam and the focal position of the manipulation laser beam are coincident, independent of the movement of the objective lens or the stage by a focusing mechanism; and in an observation mode, the focal-position adjusting unit is controlled so as to cancel out the shift of the focal position of the manipulation laser beam according to the movement of the objective lens or the stage.

摘要翻译： 用于观察样本的观察激光束和用于操纵样本的操纵激光束被多路复用; 复用光束经由物镜照射安装在台架上的试样; 并且在观察光轴方向检测从样本内部发出的荧光。 在准备模式中，控制焦点位置调节单元，使得观察激光束的焦点位置和操纵激光束的焦点位置重合，而与物镜或舞台的移动无关，由聚焦机构 ; 并且在观察模式中，控制焦点位置调整单元，以便根据物镜或舞台的移动抵消操纵激光束的焦点位置的偏移。

公开(公告)号：US07417211B2

公开(公告)日：2008-08-26

申请号：US11252564

申请日：2005-10-18

申请人： Tatsuo Nakata ,  Masahiro Oba

发明人： Tatsuo Nakata ,  Masahiro Oba

IPC分类号： G02B7/04 ,  G02B21/00 ,  F21V9/16

CPC分类号： G02B21/0064 ,  G02B21/0076 ,  G02B21/0084

摘要： A laser scanning microscope which focuses light beams from a laser beam source to a sample by means of an objective lens and detects transmission light from the sample, reflection light, or fluorescence generated from the sample, includes an observation laser scanning optical system which irradiates coherent light from one side of the sample and which carries out scanning the sample, a stimulation laser scanning optical system which irradiates coherent light from an opposite side across the sample and which carries out scanning the sample, an observation light detector provided to be branched from the observation laser scanning optical system, and a light invasion preventing section which prevents the coherent light irradiated from the stimulation laser scanning optical system from invading the observation light detector.

摘要翻译： 一种激光扫描显微镜，其将来自激光束源的光束通过物镜聚焦到样品，并检测来自样品的透射光，反射光或从样品产生的荧光，其包括观察激光扫描光学系统，其照射相干 来自样品一侧的光并进行扫描样品的激光激光扫描光学系统，其对来自相反侧的相干光照射到样品上并执行扫描样品;观察光检测器，其从 观察激光扫描光学系统和防止从刺激激光扫描光学系统照射的相干光侵入观察光检测器的光入射防止部。