公开(公告)号：US08603780B2

公开(公告)日：2013-12-10

申请号：US13550120

申请日：2012-07-16

申请人： Zhuying Wang ,  Xiaowu Liu

发明人： Zhuying Wang ,  Xiaowu Liu

IPC分类号： A61K38/00

CPC分类号： C12N15/67 ,  C07K16/32 ,  C07K2317/10 ,  C07K2319/02 ,  C12P21/02

摘要： Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described.

摘要翻译： 描述了用于增强来自细胞的蛋白质的表达和分泌的优化的信号肽编码序列以及相关的组合物和方法。 优化的信号肽编码序列编码在起始密码子的紧下游的至少一个发夹结构的mRNA。 还描述了使用优化的信号肽编码序列获得优化的信号肽编码序列的方法和用于增强蛋白质表达和分泌的方法。

公开(公告)号：US08326547B2

公开(公告)日：2012-12-04

申请号：US12894401

申请日：2010-09-30

申请人： Xiaowu Liu ,  Yun He ,  Zhuying Wang ,  Chunjiao Wang ,  Zhibing Liu ,  Tianhui Xia ,  Luquan Wang ,  Fang Liang Zhang

发明人： Xiaowu Liu ,  Yun He ,  Zhuying Wang ,  Chunjiao Wang ,  Zhibing Liu ,  Tianhui Xia ,  Luquan Wang ,  Fang Liang Zhang

IPC分类号： G06F19/10 ,  G06F19/00

CPC分类号： G06F19/22 ,  G06F19/20

摘要： An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

摘要翻译： 描述了改进的基因序列优化方法，该系统优化方法用于增强细菌，酵母，昆虫和哺乳动物细胞中基因的重组表达。 这种一般方法考虑了影响蛋白质表达的参数和因子的多个，优选大多数或全部，包括密码子使用，tRNA使用，GC含量，核糖体结合序列，启动子，5'-UTR，ORF和3'-UTR 改进和优化基因序列以增强细菌，酵母，昆虫和哺乳动物细胞中基因的蛋白质表达的基因序列。 特别地，本发明涉及使用粒子群优化算法改进重组蛋白表达的序列优化的系统和方法。 改进的系统优化方法可以被并入软件中以进行更有效的优化。