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公开(公告)号:US07851679B2
公开(公告)日:2010-12-14
申请号:US12180180
申请日:2008-07-25
CPC分类号: C12N15/8289 , C07K14/415 , C12N9/22 , C12N15/8205 , C12N15/823 , C12N15/8231 , C12N15/8233 , C12N15/8234 , C12N15/8263 , C12N15/827 , C12N15/829
摘要: The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
摘要翻译: 本发明涉及生殖发育的调节,特别涉及被子植物和裸子植物物种中生殖组织的遗传消融。 生殖优选的启动子,调节元件和细胞毒性核苷酸序列在本文中公开,以及用于遗传消融的构建体和方法。
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公开(公告)号:US07807880B2
公开(公告)日:2010-10-05
申请号:US12185623
申请日:2008-08-04
申请人: Richard L. Forster , William H. Rottmann , Marie B. Connett , Paul Sanders , Gary Zhang , Sandra Joanne Fitzgerald , Clare Eagleton
发明人: Richard L. Forster , William H. Rottmann , Marie B. Connett , Paul Sanders , Gary Zhang , Sandra Joanne Fitzgerald , Clare Eagleton
CPC分类号: C12N9/0073 , C12N15/8255
摘要: DNA constructs comprising a first DNA segment that corresponds to at least a portion of a gene in the monolignol biosynthetic pathway, a spacer DNA segment, and a second DNA segment that is complementary to the first DNA segment can be used to reduce or modulate the lignin content in plants. In some embodiments, DNA constructs comprise at least a portion of a gene for 4CL, C3H, CCR, C4H or CCoAOMT. Vascular-preferred and constitutive promoters can be used to drive expression of the constructs.
摘要翻译: 包含对应于单螺旋藻生物合成途径中的基因的至少一部分的第一DNA片段,间隔区DNA片段和与第一DNA片段互补的第二DNA片段的DNA构建体可用于减少或调节木质素 植物中的内容。 在一些实施方案中,DNA构建体包含用于4CL,C3H,CCR,C4H或CCoAOMT的基因的至少一部分。 血管优选和组成型启动子可用于驱动构建体的表达。
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公开(公告)号:US20090229008A1
公开(公告)日:2009-09-10
申请号:US12187745
申请日:2008-08-07
申请人: LEONARD N. BLOKSBERG , CATHERINE BRYANT , MARIE B. CONNETT , SARAH JANE EMERSON , J. FROST , RICHARD LLEWELLYN SYDNEY FORSTER , MURRAY GRIGOR , COLLEEN HIGGINS , ANNETTE LASHAM , STEVEN TROY LUND , ANDREAS MAGUSIN , JONATHAN PHILLIPS , SATHIAH PUTHIGAE , STELLA VEERAKONE , CLAIRE WESTWOOD , KATRINA GAUSE , MARION WOOD , ILKKA HAVUKKALA , WILLIAM H. ROTTMANN
发明人: LEONARD N. BLOKSBERG , CATHERINE BRYANT , MARIE B. CONNETT , SARAH JANE EMERSON , J. FROST , RICHARD LLEWELLYN SYDNEY FORSTER , MURRAY GRIGOR , COLLEEN HIGGINS , ANNETTE LASHAM , STEVEN TROY LUND , ANDREAS MAGUSIN , JONATHAN PHILLIPS , SATHIAH PUTHIGAE , STELLA VEERAKONE , CLAIRE WESTWOOD , KATRINA GAUSE , MARION WOOD , ILKKA HAVUKKALA , WILLIAM H. ROTTMANN
CPC分类号: C12N15/8261 , C07K14/415 , C12N15/8255 , Y02A40/146
摘要: The invention provides polynucleotide sequences isolated from plants encoding transcription factors. Polypeptides encoded by the polynucleotides are also provided. Products and methods of use are disclosed.
摘要翻译: 本发明提供从编码转录因子的植物分离的多核苷酸序列 还提供了由多核苷酸编码的多肽。 公开了产品和使用方法。
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公开(公告)号:US20090038033A1
公开(公告)日:2009-02-05
申请号:US12096516
申请日:2006-12-06
申请人: Leonard N. Bloksberg , Michael J. Frost , Richard Forster , Colleen Higgins , William H. Rottmann , Kim Norris-Caneda
发明人: Leonard N. Bloksberg , Michael J. Frost , Richard Forster , Colleen Higgins , William H. Rottmann , Kim Norris-Caneda
CPC分类号: C07K14/415 , C12N15/8261 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , Y02A40/146
摘要: The invention provides polynucleotide and polypeptide sequences isolated from P. radiate and E. grandis that are involved in wood and cell wall biosynthesis. Methods for using the sequences, along with constructs and transgenic plants, are provided also.
摘要翻译: 本发明提供了分离自P. radiate和E. grandis的参与木材和细胞壁生物合成的多核苷酸和多肽序列。 还提供了使用序列以及构建体和转基因植物的方法。
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公开(公告)号:US07453025B2
公开(公告)日:2008-11-18
申请号:US10946622
申请日:2004-09-22
CPC分类号: C12N15/8289 , C07K14/415 , C12N9/22 , C12N15/8205 , C12N15/823 , C12N15/8231 , C12N15/8233 , C12N15/8234 , C12N15/8263 , C12N15/827 , C12N15/829
摘要: The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
摘要翻译: 本发明涉及生殖发育的调节,特别涉及被子植物和裸子植物物种中生殖组织的遗传消融。 生殖优选的启动子,调节元件和细胞毒性核苷酸序列在本文中公开,以及用于遗传消融的构建体和方法。
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公开(公告)号:US20060101537A1
公开(公告)日:2006-05-11
申请号:US11158342
申请日:2005-06-22
申请人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
发明人: Shujun Chang , Robert Thomas , Levis Handley , Marie Connett , Randy Hamilton
CPC分类号: C12N15/8205
摘要: The present invention relates to a method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of an Agrobacterium inducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenic E. grandis×E. urophylla cells and therefrom regenerating stably transformed E. grandis×E. urophylla trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants.
摘要翻译: 本发明涉及一种用于转化和选择植物外植体的方法。 转化方法包括在农杆菌诱导物存在下预培养外植体,并将转化的外植体暴露于加速芽发育的芽再生培养基中。 提供了从该转化方法产生的植物。 特别地,获得转基因大蠊的方法。 桉树细胞,从而再生稳定转化的大x x。 提供桉树。 本发明还提供用于选择和再生植物的培养基,方法和质粒。
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公开(公告)号:US20050244968A1
公开(公告)日:2005-11-03
申请号:US10927641
申请日:2004-08-27
申请人: Ranjan Perera , Stephen Rice , Clare Eagleton , Marion Wood , Elizabeth Visser
发明人: Ranjan Perera , Stephen Rice , Clare Eagleton , Marion Wood , Elizabeth Visser
IPC分类号: A01H1/00 , A01H5/00 , C07K14/415 , C12N9/00 , C12N15/82
CPC分类号: C07K14/415 , C12N9/00 , C12N15/8216 , C12N15/8222 , C12N15/8223 , C12N15/8225 , C12N15/8227 , C12N15/8229 , C12N15/8231 , C12N15/8233
摘要: Novel isolated plant polynucleotide promoter sequences are provided, together with genetic constructs comprising such polynucleotides. Methods for using such constructs in modulating the transcription of DNA sequences of interest are also disclosed, together with transgenic plants comprising such constructs.
摘要翻译: 提供了新的分离的植物多核苷酸启动子序列,以及包含这种多核苷酸的遗传构建体。 还公开了使用这种构建体调节感兴趣的DNA序列的转录的方法,以及包含这些构建体的转基因植物。
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公开(公告)号:US20040163146A1
公开(公告)日:2004-08-19
申请号:US10717897
申请日:2003-11-21
申请人: Arborgen, LLC
发明人: Jonathan Phillips , Sathish Puthigae , JiaLong Yao , Barry Flinn , Richard S. Forster , Clare Eagleton
IPC分类号: A01H001/00 , C12N015/82 , C07H021/04 , C12N015/87 , C12N005/04
CPC分类号: C12N15/8223 , C12N15/8226
摘要: The present invention relates to the regulation of polynucleotide transcription and/or expression. In particular, this invention relates to polynucleotide regulatory sequences isolated from Eucalyptus grandis and Pinus radiata that are capable of conferring vascular-preferred polynucleotide transcription in plant cells. Constructs and methods for using the inventive regulatory sequences for modifying transcription of endogenous and/or heterologous polynucleotides also are included in the invention.
摘要翻译: 本发明涉及多核苷酸转录和/或表达的调控。 特别地,本发明涉及从能够在植物细胞中赋予血管优选的多核苷酸转录的从桉树(Eucalyptus grandis)和松属(Pinus radiata)分离的多核苷酸调节序列。 本发明还包括使用本发明调节序列修饰内源和/或异源多核苷酸转录的构建体和方法。
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公开(公告)号:US08017833B2
公开(公告)日:2011-09-13
申请号:US12096516
申请日:2006-12-06
申请人: Leonard N. Bloksberg , Michael J. Frost , Richard Forster , Colleen Higgins , William H. Rottmann , Kim Norris-Caneda
发明人: Leonard N. Bloksberg , Michael J. Frost , Richard Forster , Colleen Higgins , William H. Rottmann , Kim Norris-Caneda
IPC分类号: C12N15/113 , C12N15/82 , C12N15/63 , A01H5/04 , C07H21/04
CPC分类号: C07K14/415 , C12N15/8261 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , Y02A40/146
摘要: The invention provides polynucleotide and polypeptide sequences isolated from P. radiate and E. grandis that are involved in wood and cell wall biosynthesis. Methods for using the sequences, along with constructs and transgenic plants, are provided also.
摘要翻译: 本发明提供了分离自P. radiate和E. grandis的参与木材和细胞壁生物合成的多核苷酸和多肽序列。 还提供了使用序列以及构建体和转基因植物的方法。
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公开(公告)号:US20110107456A1
公开(公告)日:2011-05-05
申请号:US12962190
申请日:2010-12-07
CPC分类号: C12N15/8289 , C07K14/415 , C12N9/22 , C12N15/8205 , C12N15/823 , C12N15/8231 , C12N15/8233 , C12N15/8234 , C12N15/8263 , C12N15/827 , C12N15/829
摘要: The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
摘要翻译: 本发明涉及生殖发育的调节,特别涉及被子植物和裸子植物物种中生殖组织的遗传消融。 生殖优选的启动子,调节元件和细胞毒性核苷酸序列在本文中公开,以及用于遗传消融的构建体和方法。
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