公开(公告)号：US20100254590A1

公开(公告)日：2010-10-07

申请号：US12817422

申请日：2010-06-17

申请人： PETER RAMM ,  YURIY ALEXANDROV ,  JURICH CYBUCH ,  PAUL DONDERS ,  CARLOS ZARATE ,  BOHDAN J. SOLTYS

发明人： PETER RAMM ,  YURIY ALEXANDROV ,  JURICH CYBUCH ,  PAUL DONDERS ,  CARLOS ZARATE ,  BOHDAN J. SOLTYS

IPC分类号： G06T7/00

CPC分类号： G01N21/6458 ,  G01N15/1475 ,  G01N21/6428 ,  G01N33/5026 ,  G01N2015/1497 ,  G01N2333/4603 ,  G06K9/00127 ,  G06T7/0012 ,  G06T7/187 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A system for performing automated cell screening in drug discovery, includes an automated microscope, a fast autofocus device, and a digital imaging system. Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known to methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.

摘要翻译： 用于在药物发现中进行自动细胞筛选的系统包括自动显微镜，快速自动对焦装置和数字成像系统。 过程在软件中实现，通过该软件，相关的细胞材料通过最小的用户交互被分段和量化。 在图像处理方法中已知的以下领域的改进以实现自动分割的方式实现; 已知测量的集合（像素计数等）被实现为以可靠的方式示出生物学方面的方法; 将多个样本的自动定位，聚焦，成像和处理的组件集成为其中可以安装分割和测量方法的系统; 并且组件和方法被适应成产生更高度自动化和更快速的细胞筛选的系统。

公开(公告)号：US09057701B2

公开(公告)日：2015-06-16

申请号：US12817422

申请日：2010-06-17

申请人： Peter Ramm ,  Yuriy Alexandrov ,  Paul Donders ,  Carlos Zarate ,  Bohdan J. Soltys

发明人： Peter Ramm ,  Yuriy Alexandrov ,  Jurich Cybuch ,  Paul Donders ,  Carlos Zarate ,  Bohdan J. Soltys

IPC分类号： G06K9/00 ,  G01N21/64 ,  G01N15/14 ,  G01N33/50 ,  G06T7/00

CPC分类号： G01N21/6458 ,  G01N15/1475 ,  G01N21/6428 ,  G01N33/5026 ,  G01N2015/1497 ,  G01N2333/4603 ,  G06K9/00127 ,  G06T7/0012 ,  G06T7/187 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A system for performing automated cell screening in drug discovery, includes an automated microscope, a fast autofocus device, and a digital imaging system. Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.

摘要翻译： 用于在药物发现中进行自动细胞筛选的系统包括自动显微镜，快速自动对焦装置和数字成像系统。 过程在软件中实现，通过该软件，相关的细胞材料通过最小的用户交互被分段和量化。 以下领域的改进：图像处理的已知方法以实现自动分割的方式实现; 已知测量的集合（像素计数等）被实现为以可靠的方式示出生物学方面的方法; 将多个样本的自动定位，聚焦，成像和处理的组件集成为其中可以安装分割和测量方法的系统; 并且组件和方法被适应成产生更高度自动化和更快速的细胞筛选的系统。

公开(公告)号：US08885911B2

公开(公告)日：2014-11-11

申请号：US13277324

申请日：2011-10-20

申请人： Xudong Li ,  Louis Ernest Dagenais ,  William Mark Durksen ,  Del Archer

发明人： Xudong Li ,  Louis Ernest Dagenais ,  William Mark Durksen ,  Del Archer

IPC分类号： G06K9/00

CPC分类号： G06K9/00127

摘要： A computer-based method for the development of an image analysis protocol for analyzing image data, the image data containing images including image objects, in particular biological image objects such as biological cells. The image analysis protocol, once developed, is operable in an image analysis software system to report on one or more measurements conducted on selected ones of the image objects. The development process includes defining target identification settings to identify at least two different target sets of image objects, defining target identification settings to identify at least two different target sets of image objects, and defining one or more measurements to be performed using said pair-wise linking relationship(s).

摘要翻译： 一种用于开发用于分析图像数据的图像分析协议的基于计算机的方法，所述图像数据包含包括图像对象的图像，特别是生物图像对象，例如生物细胞。 一旦开发出图像分析协议，在图像分析软件系统中可操作以报告对所选择的图像对象进行的一个或多个测量。 开发过程包括定义目标识别设置以识别图像对象的至少两个不同的目标集合，定义目标识别设置以识别图像对象的至少两个不同目标集合，以及使用所述成对方式来定义要执行的一个或多个测量 链接关系。

公开(公告)号：US20090074283A1

公开(公告)日：2009-03-19

申请号：US12253275

申请日：2008-10-17

申请人： PETER RAMM ,  Yuriy Alexandrov ,  Jurich Cybuch ,  Paul Donders ,  Carlos Zarate ,  Bohdan J. Soltys

发明人： PETER RAMM ,  Yuriy Alexandrov ,  Jurich Cybuch ,  Paul Donders ,  Carlos Zarate ,  Bohdan J. Soltys

IPC分类号： G06K9/00

CPC分类号： G01N21/6458 ,  G01N15/1475 ,  G01N21/6428 ,  G01N33/5026 ,  G01N2015/1497 ,  G01N2333/4603 ,  G06K9/00127 ,  G06T7/0012 ,  G06T7/187 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A system for performing automated cell screening in drug discovery, includes an automated microscope, a fast autofocus device, and a digital imaging system. Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.

摘要翻译： 用于在药物发现中进行自动细胞筛选的系统包括自动显微镜，快速自动对焦装置和数字成像系统。 过程在软件中实现，通过该软件，相关的细胞材料通过最少的用户交互被分段和量化。 以下领域的改进：图像处理的已知方法以实现自动分割的方式实现; 已知测量的集合（像素计数等）被实现为以可靠的方式示出生物学方面的方法; 将多个样本的自动定位，聚焦，成像和处理的组件集成为其中可以安装分割和测量方法的系统; 并且组件和方法被适应成产生更高度自动化和更快速的细胞筛选的系统。

公开(公告)号：US07335898B2

公开(公告)日：2008-02-26

申请号：US11184444

申请日：2005-07-19

申请人： Paul Donders ,  Carlos Zarate ,  Pavel A. Fomitchov

发明人： Paul Donders ,  Carlos Zarate ,  Pavel A. Fomitchov

IPC分类号： G01N21/64

CPC分类号： G02B21/0076 ,  G02B21/0036 ,  G02B21/0064 ,  G02B21/0084

摘要： A new and improved confocal fluorescence microscope is presented. The new microscope has significant advantages relative to existing implementations of microscope confocal imagers. In common with previous confocal imagers the instant invention has the advantages relative to conventional wide-field and confocal fluorescence imagers, however it addresses the drawbacks of confocal technology in terms of cost and complexity, and provides significant savings in both due to the simplicity of the components and the elimination of the need of, in particular, spatial filters such as pinholes or slits.

摘要翻译： 提出了一种新的改进的共焦荧光显微镜。 新显微镜相对于现有的显微镜共焦成像仪的实现具有显着的优势。 与以前的共焦成像仪相同，本发明相对于传统的宽场和共聚焦荧光成像器具有优点，但是它在成本和复杂性方面解决了共聚焦技术的缺点，并且由于简化了 组件和消除特别是诸如针孔或狭缝的空间滤波器的需要。

公开(公告)号：US20120057752A1

公开(公告)日：2012-03-08

申请号：US13277324

申请日：2011-10-20

申请人： XUDONG LI ,  LOUIS ERNEST DAGENAIS ,  WILLIAM MARK DURKSEN ,  DEL ARCHER

发明人： XUDONG LI ,  LOUIS ERNEST DAGENAIS ,  WILLIAM MARK DURKSEN ,  DEL ARCHER

IPC分类号： G06K9/00

CPC分类号： G06K9/00127

摘要： A computer-based method for the development of an image analysis protocol for analyzing image data, the image data containing images including image objects, in particular biological image objects such as biological cells. The image analysis protocol, once developed, is operable in an image analysis software system to report on one or more measurements conducted on selected ones of the image objects. The development process includes defining target identification settings to identify at least two different target sets of image objects, defining target identification settings to identify at least two different target sets of image objects, and defining one or more measurements to be performed using said pair-wise linking relationship(s).

摘要翻译： 一种用于开发用于分析图像数据的图像分析协议的基于计算机的方法，所述图像数据包含包括图像对象的图像，特别是生物图像对象，例如生物细胞。 一旦开发出图像分析协议，在图像分析软件系统中可操作以报告对所选择的图像对象进行的一个或多个测量。 开发过程包括定义目标识别设置以识别图像对象的至少两个不同的目标集合，定义目标识别设置以识别图像对象的至少两个不同目标集合，以及使用所述成对方式来定义要执行的一个或多个测量 链接关系。

公开(公告)号：US08045782B2

公开(公告)日：2011-10-25

申请号：US11019326

申请日：2004-12-07

申请人： Xudong Li ,  Louis Ernest Dagenais ,  William Mark Durksen ,  Del Archer

发明人： Xudong Li ,  Louis Ernest Dagenais ,  William Mark Durksen ,  Del Archer

IPC分类号： G06K9/00

CPC分类号： G06K9/00127

摘要： A computer-based method for the development of an image analysis protocol for analyzing image data, the image data containing images including image objects, in particular biological image objects such as biological cells. The image analysis protocol, once developed, is operable in an image analysis software system to report on one or more measurements conducted on selected ones of the image objects. The development process includes providing functions for selecting predetermined image analysis procedures, the functions allowing the user to define: at least one first target identification setting for identifying a first target set of image objects in the image data; at least one second target identification setting for identifying a second target set of image objects in the image data; a relationship between the first and second set of image objects; and one or more measurements which are to be reported for the image data, the measurements being conducted using the defined relationship.

摘要翻译： 一种用于开发用于分析图像数据的图像分析协议的基于计算机的方法，所述图像数据包含包括图像对象的图像，特别是生物图像对象，例如生物细胞。 一旦开发出图像分析协议，在图像分析软件系统中可操作以报告对所选择的图像对象进行的一个或多个测量。 开发过程包括提供用于选择预定图像分析过程的功能，允许用户定义的功能：用于识别图像数据中的图像对象的第一目标集合的至少一个第一目标识别设置; 用于识别所述图像数据中的图像对象的第二目标集合的至少一个第二目标识别设置; 第一和第二组图像对象之间的关系; 以及要针对图像数据报告的一个或多个测量值，使用定义的关系进行测量。

公开(公告)号：US07764822B2

公开(公告)日：2010-07-27

申请号：US12253275

申请日：2008-10-17

申请人： Peter Ramm ,  Yuriy Alexandrov ,  Jurich Cybuch ,  Paul Donders ,  Carlos Zarate ,  Bohdan Soltys

发明人： Peter Ramm ,  Yuriy Alexandrov ,  Jurich Cybuch ,  Paul Donders ,  Carlos Zarate ,  Bohdan Soltys

IPC分类号： G06K9/00

CPC分类号： G01N21/6458 ,  G01N15/1475 ,  G01N21/6428 ,  G01N33/5026 ,  G01N2015/1497 ,  G01N2333/4603 ,  G06K9/00127 ,  G06T7/0012 ,  G06T7/187 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A system for performing automated cell screening in drug discovery, includes an automated microscope, a fast autofocus device, and a digital imaging system. Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.

摘要翻译： 用于在药物发现中进行自动细胞筛选的系统包括自动显微镜，快速自动对焦装置和数字成像系统。 过程在软件中实现，通过该软件，相关的细胞材料通过最小的用户交互被分段和量化。 以下领域的改进：图像处理的已知方法以实现自动分割的方式实现; 已知测量的集合（像素计数等）被实现为以可靠的方式示出生物学方面的方法; 将多个样本的自动定位，聚焦，成像和处理的组件集成为其中可以安装分割和测量方法的系统; 并且组件和方法被适应成产生更高度自动化和更快速的细胞筛选的系统。

公开(公告)号：US07469056B2

公开(公告)日：2008-12-23

申请号：US10514925

申请日：2003-05-09

申请人： Peter Ramm ,  Yuriy Alexandrov ,  Jurich Cybuch ,  Paul Donders ,  Carlos Zarate ,  Bohdan J. Soltys

发明人： Peter Ramm ,  Yuriy Alexandrov ,  Jurich Cybuch ,  Paul Donders ,  Carlos Zarate ,  Bohdan J. Soltys

IPC分类号： G06K9/00

CPC分类号： G01N21/6458 ,  G01N15/1475 ,  G01N21/6428 ,  G01N33/5026 ,  G01N2015/1497 ,  G01N2333/4603 ,  G06K9/00127 ,  G06T7/0012 ,  G06T7/187 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A system for performing automated cell screening in drug discovery, including an automated microscope, a fast autofocus device and a digital imaging system. Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.

摘要翻译： 一种用于在药物发现中进行自动细胞筛选的系统，包括自动显微镜，快速自动对焦装置和数字成像系统。 过程在软件中实现，通过该软件，相关的细胞材料通过最小的用户交互被分段和量化。 以下领域的改进：图像处理的已知方法以实现自动分割的方式实现; 已知测量的集合（像素计数等）被实现为以可靠的方式示出生物学方面的方法; 将多个样本的自动定位，聚焦，成像和处理的组件集成为其中可以安装分割和测量方法的系统; 并且组件和方法被适应成产生更高度自动化和更快速的细胞筛选的系统。