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公开(公告)号：US5515169A
公开(公告)日：1996-05-07
申请号：US136212
申请日：1993-10-13
CPC分类号： G01N21/255 , G01J3/26 , G01J9/0246 , G01J3/0294
摘要： A spectral wavelength discrimination system and method for using are provided which allows the wavelength of a beam of radiation to be accurately determined using compact inexpensive optics and electronics. The system is particularly useful for identifying the emission wavelength of a multi-component marker system which includes a plurality of components having different wavelength ranges. The system comprises a wavelength selective beamsplitter, termed a Linear Wavelength Filter, that directs predetermined fractions of the beam at each wavelength into each of two output beams. The intensities of these output beams are measured. The measurements and selected system parameters, including the beamsplitter spectral characteristics and the detector sensitivity characteristics are used in a special algorithm for performing Fourier based wavelength-dispersive analysis. The unique solution of the Fourier based analysis is the wavelength of the beam of radiation. The system employs various optical components and structures to achieve the desired spatial resolution and sensitivity. Multiple channel devices and other special configurations are also described.
摘要翻译： 提供了一种使用光谱波长鉴别系统和方法，其允许使用紧凑廉价的光学和电子学来精确地确定辐射束的波长。 该系统对于识别包括具有不同波长范围的多个分量的多分量标记系统的发射波长特别有用。 该系统包括称为线性波长滤波器的波长选择性分束器，其将每个波长处的波束的预定分数引导到两个输出波束中的每一个。 测量这些输出光束的强度。 用于执行基于傅立叶的波长色散分析的特殊算法中使用的测量和选定的系统参数，包括分光器光谱特性和检测器灵敏度特性。 傅里叶分析的独特解决方案是辐射束的波长。 该系统采用各种光学部件和结构来实现所需的空间分辨率和灵敏度。 还描述了多信道设备和其他特殊配置。
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公开(公告)号：US5104512A
公开(公告)日：1992-04-14
申请号：US522325
申请日：1990-05-14
IPC分类号： G01N27/447
CPC分类号： G01N27/44721 , G01N27/44704 , G01N27/44782
摘要： An electrophoretic system is provided which allows for carrying out electrophoresis while monitoring and regulating the temperature and the electrical field gradient in the gel. In addition, photometric monitoring is provided so as to monitor the progress of the electrophoretic separation and vary conditions to change the progress as desired. A computer is employed which receives the signals from the electrophoretic and photometric apparatuses and regulates temperature and voltage to either maintain conditions, or change the conditions to vary the progress of the electrophoresis. Gel molds are provided for forming the lanes in a gel plate, as well as a light module, for reading the bands present in the gel lanes with the photometer.
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公开(公告)号：US5667654A
公开(公告)日：1997-09-16
申请号：US606454
申请日：1996-02-23
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44773 , G01N27/44726
摘要： Methods are provided for assaying electrophoretically separated components of sample in a gel. Following an initial separation phase of the sample components into distinct bands within separate zones of the gel, the separated components are held stationary on the gel in a hold mode. During the hold mode, reagent required for an intended assay is applied to the zone of the gel comprising the component to be assayed. The assay is conducted during the hold-mode. A wide range of assays can be conducted on the components in the gel, without the need for a blotting step. Following completion of the assay, the hold mode is removed and, if desired, the sample components are further separated in the gel.
摘要翻译： 提供了用于测定凝胶中样品的电泳分离组分的方法。 在将样品组分初始分离到凝胶的不同区域内的不同条带之前，分离的组分在保持模式下保持在凝胶上。 在保持模式期间，将期望测定所需的试剂施加到包含要测定的组分的凝胶区域。 在保持模式期间进行测定。 可以对凝胶中的组分进行广泛的测定，而不需要印迹步骤。 完成测定后，除去保持模式，如果需要，样品组分在凝胶中进一步分离。
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4.发明授权Gel electrophoresis system including optical stage, sample applicator and sample retriever 失效凝胶电泳系统，包括光学平台，样品涂布器和样品回收器
公开(公告)号：US5275710A
公开(公告)日：1994-01-04
申请号：US837067
申请日：1992-02-14
申请人： Erich A. Gombocz , Alex T. Roth
发明人： Erich A. Gombocz , Alex T. Roth
IPC分类号： G01N27/447 , G01N27/26 , B01D57/02
CPC分类号： G01N27/44704 , G01N27/44721 , G01N27/44782
摘要： An electrophoretic system is provided which allows for carrying out electrophoresis while monitoring and regulating the temperature and the electrical field gradient in the gel. In addition, photometric monitoring, including use of an optical stage, is provided so as to monitor the progress of the electrophoretic separation and vary conditions to change the progress as desired. A computer is employed which receives the signals from the electrophoretic and photometric apparatuses and regulates temperature and voltage to either maintain conditions, or change the conditions to vary the progress of the electrophoresis and to store the separation results. A sample applicator and a sample retriever are also provided, to provide even and consistent application of sample to the gel and to allow recovery of portions of the sample without destroying or removing pieces from the gel. Gel molds are provided for forming the lanes in a gel plate, as well as a light module, for reading the bands present in the gel lanes with the photometer.
摘要翻译： 提供了一种电泳系统，其允许进行电泳，同时监测和调节凝胶中的温度和电场梯度。 此外，提供包括使用光学级的光度监测，以监视电泳分离的进展并改变条件以根据需要改变进展。 接收来自电泳和测光装置的信号并调节温度和电压以维持条件或改变条件以改变电泳进程并存储分离结果的计算机。 还提供样品施加器和样品回收器，以将样品均匀且一致地应用于凝胶并允许回收部分样品而不破坏或从凝胶中除去碎片。 提供凝胶模具用于在凝胶板中形成通道以及光模块，用于通过光度计读取凝胶通道中存在的条带。
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公开(公告)号：US5217591A
公开(公告)日：1993-06-08
申请号：US954911
申请日：1992-09-30
申请人： Erich Gombocz , Mark Dwight , David H. Rammler
发明人： Erich Gombocz , Mark Dwight , David H. Rammler
IPC分类号： G01N27/447
CPC分类号： G01N27/44704 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： A sample applicator/retriever for gel electrophoresis is provided, which allows easy and reproducible application or retrieval of samples. The sample nozzle is designed to load samples uniformly through a gel. The applicator/retriever may be in several different forms, including a a multiwell device, individual wells and mounting bar, a single line well, or a concentrator applicator.
摘要翻译： 提供了用于凝胶电泳的样品施加器/回收器，其允许容易且可再现的样品的应用或检索。 样品喷嘴设计为通过凝胶均匀地加载样品。 施放器/回收器可以是几种不同的形式，包括多孔装置，单独的井和安装杆，单线井或集中器施加器。
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