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公开(公告)号：US5667654A
公开(公告)日：1997-09-16
申请号：US606454
申请日：1996-02-23
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44773 , G01N27/44726
摘要： Methods are provided for assaying electrophoretically separated components of sample in a gel. Following an initial separation phase of the sample components into distinct bands within separate zones of the gel, the separated components are held stationary on the gel in a hold mode. During the hold mode, reagent required for an intended assay is applied to the zone of the gel comprising the component to be assayed. The assay is conducted during the hold-mode. A wide range of assays can be conducted on the components in the gel, without the need for a blotting step. Following completion of the assay, the hold mode is removed and, if desired, the sample components are further separated in the gel.
摘要翻译： 提供了用于测定凝胶中样品的电泳分离组分的方法。 在将样品组分初始分离到凝胶的不同区域内的不同条带之前，分离的组分在保持模式下保持在凝胶上。 在保持模式期间，将期望测定所需的试剂施加到包含要测定的组分的凝胶区域。 在保持模式期间进行测定。 可以对凝胶中的组分进行广泛的测定，而不需要印迹步骤。 完成测定后，除去保持模式，如果需要，样品组分在凝胶中进一步分离。
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公开(公告)号：US5104512A
公开(公告)日：1992-04-14
申请号：US522325
申请日：1990-05-14
IPC分类号： G01N27/447
CPC分类号： G01N27/44721 , G01N27/44704 , G01N27/44782
摘要： An electrophoretic system is provided which allows for carrying out electrophoresis while monitoring and regulating the temperature and the electrical field gradient in the gel. In addition, photometric monitoring is provided so as to monitor the progress of the electrophoretic separation and vary conditions to change the progress as desired. A computer is employed which receives the signals from the electrophoretic and photometric apparatuses and regulates temperature and voltage to either maintain conditions, or change the conditions to vary the progress of the electrophoresis. Gel molds are provided for forming the lanes in a gel plate, as well as a light module, for reading the bands present in the gel lanes with the photometer.
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公开(公告)号：US5410412A
公开(公告)日：1995-04-25
申请号：US178035
申请日：1994-01-06
IPC分类号： G01N27/447 , G01N21/25
CPC分类号： G01N27/44721 , G01N27/44704 , G01N27/44782
摘要： An electrophoretic system is provided which allows for carrying out electrophoresis while monitoring and regulating the temperature and the electrical field gradient in the gel. In addition, photometric monitoring is provided so as to monitor the progress of the electrophoretic separation and vary conditions to change the progress as desired. A computer is employed which receives the signals from the electrophoretic and photometric apparatuses and regulates temperature and voltage to either maintain conditions, or change the conditions to vary the progress of the electrophoresis. Gel molds are provided for forming the lanes in a gel plate, as well as a light module, for reading the bands present in the gel lanes with the photometer.
摘要翻译： 提供了一种电泳系统，其允许进行电泳，同时监测和调节凝胶中的温度和电场梯度。 此外，提供光度监测以监测电泳分离的进展并改变条件以根据需要改变进展。 使用计算机，其接收来自电泳和光度测量装置的信号并调节温度和电压以维持条件，或改变条件以改变电泳进程。 提供凝胶模具用于在凝胶板中形成通道以及光模块，用于通过光度计读取凝胶通道中存在的条带。
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公开(公告)号：US5472427A
公开(公告)日：1995-12-05
申请号：US142192
申请日：1993-10-22
申请人： David H. Rammler
发明人： David H. Rammler
CPC分类号： A61B17/0281 , A61B17/3403 , A61B17/3476 , A61B17/3494 , A61B17/085 , A61B17/3468 , A61B2017/00022 , A61B2017/00106 , A61B2017/00473 , A61B2017/00539 , A61B2017/347 , A61B2017/3492 , A61B2046/205 , A61B2090/034 , A61B2090/062 , A61B2090/08021 , A61B2090/378 , A61B2090/3788 , A61B90/03
摘要： A trocar device is disclosed which is adapted for insertion into a body cavity, such as the abdominal cavity, without the need for cavity insufflation. The trocar device comprises applicator and trocar components. The trocar components comprises a cylindrical trocar tube and detachable obdurator. The applicator comprises a housing into which the trocar and obdurator are loaded prior to insertion, and means for applying a force, such as a spring or electric motor, to drive the obdurator and trocar through the body wall into the cavity. Various electrical and mechanical means are provided for limiting the penetration depth of the obdurator. In one embodiment, sonic transducer means are provided for detecting a phase change in reflected sonic waves at the internal body wall boundary to stop trocar penetration. Several alternative embodiments of the apparatus and method of use are disclosed.
摘要翻译： 公开了一种套管针装置，其适于插入诸如腹腔的体腔中，而不需要空腔吹入。 针灸针装置包括敷抹器和套管针组件。 套管针组件包括圆柱形套管针管和可拆卸的牙膏。 施用器包括在插入之前套管针和套筒装载的壳体，以及用于施加力的装置，例如弹簧或电动马达，以将吸引器和套针穿过体壁进入空腔。 提供了各种电气和机械装置来限制胶囊的穿透深度。 在一个实施例中，提供了用于检测内部体壁边界处反射的声波的相位变化的声音换能器装置，以停止套管针穿透。 公开了该设备和使用方法的几个替代实施例。
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公开(公告)号：US5769820A
公开(公告)日：1998-06-23
申请号：US518584
申请日：1995-08-23
申请人： David H. Rammler
发明人： David H. Rammler
CPC分类号： A61B17/0281 , A61B17/3403 , A61B17/3476 , A61B17/3494 , A61B17/085 , A61B17/3468 , A61B2017/00022 , A61B2017/00106 , A61B2017/00473 , A61B2017/00539 , A61B2017/347 , A61B2017/3492 , A61B2046/205 , A61B2090/034 , A61B2090/062 , A61B2090/08021 , A61B2090/378 , A61B2090/3788 , A61B90/03
摘要： A trocar system comprising a rack apparatus and a trocar device are disclosed. The trocar device is adapted for insertion into a body cavity, such as the abdominal cavity, using the rack apparatus which eliminates the need for cavity insufflation. The rack apparatus comprises a movable member which attaches adhesively to the external skin surface of the body wall overlying the body cavity. Means are provided moving the member attached to the skin away from the body cavity in order to lift the abdominal region and create a separation between the body wall and the underlying internal organs. This separation permits the obdurator portion of a trocar device to be inserted with a minimum risk of cutting any internal organs. The trocar device comprises applicator and trocar components. The trocar components comprises a cylindrical trocar tube and detachable obdurator. The applicator comprises a housing into which the trocar and obdurator are loaded prior to insertion, and means for applying a force, such as a spring or electric motor, to drive the obdurator and trocar through the body wall into the cavity. Various electrical and mechanical means are provided for limiting the penetration depth of the obdurator.
摘要翻译： 公开了一种包括齿条装置和套针针装置的套管针系统。 套针针装置适于使用不需要空腔吹入的齿条装置插入诸如腹腔的体腔内。 机架装置包括可移动部件，该可移动部件粘附到与体腔重叠的体壁的外表皮表面。 提供了使附接到皮肤的构件远离体腔移动的装置，以便抬起腹部区域并在体壁和下面的内部器官之间产生分离。 这种分离允许套管针装置的脏物部分以最小的切割任何内部器官的风险被插入。 针灸针装置包括敷抹器和套管针组件。 套管针组件包括圆柱形套管针管和可拆卸的牙膏。 施用器包括在插入之前套管针和套筒装载的壳体，以及用于施加力的装置，例如弹簧或电动马达，以驱动吸引器和套针穿过体壁进入空腔。 提供了各种电气和机械装置来限制胶囊的穿透深度。
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公开(公告)号：US5688243A
公开(公告)日：1997-11-18
申请号：US518561
申请日：1995-08-23
申请人： David H. Rammler
发明人： David H. Rammler
CPC分类号： A61B17/0281 , A61B17/3403 , A61B17/3476 , A61B17/3494 , A61B17/085 , A61B17/3468 , A61B2017/00022 , A61B2017/00106 , A61B2017/00473 , A61B2017/00539 , A61B2017/347 , A61B2017/3492 , A61B2046/205 , A61B2090/034 , A61B2090/062 , A61B2090/08021 , A61B2090/378 , A61B2090/3788 , A61B90/03
摘要： A surgical method involving a trocar system comprising a rack apparatus and a trocar device is disclosed. The trocar device is adapted for insertion into a body cavity, such as the abdominal cavity, using the rack apparatus which eliminates the need for cavity insufflation. The rack apparatus comprises a movable member which attaches adhesively to the external skin surface of the body wall overlying the body cavity. Means are provided moving the member attached to the skin away from the body cavity in order to lift the abdominal region and create a separation between the body wall and the underlying internal organs. This separation permits the obdurator portion of a trocar device to be inserted with a minimum risk of cutting any internal organs. The trocar device comprises applicator and trocar components. The trocar components comprises a cylindrical trocar tube and detachable obdurator. The applicator comprises a housing into which the trocar and obdurator are loaded prior to insertion, and means for applying a force, such as a spring or electric motor, to drive the obdurator and trocar through the body wall into the cavity. Various electrical and mechanical means are provided for limiting the penetration depth of the obdurator.
摘要翻译： 公开了一种涉及包括齿条装置和套针针装置的套管针系统的手术方法。 套针针装置适于使用不需要空腔吹入的齿条装置插入诸如腹腔的体腔内。 机架装置包括可移动部件，该可移动部件粘附到与体腔重叠的体壁的外表皮表面。 提供了使附接到皮肤的构件远离体腔移动的装置，以便抬起腹部区域并在体壁和下面的内部器官之间产生分离。 这种分离允许套管针装置的脏物部分以最小的切割任何内部器官的风险被插入。 针灸针装置包括敷抹器和套管针组件。 套管针组件包括圆柱形套管针管和可拆卸的牙膏。 施用器包括在插入之前套管针和套筒装载的壳体，以及用于施加力的装置，例如弹簧或电动马达，以驱动吸引器和套针穿过体壁进入空腔。 提供了各种电气和机械装置来限制胶囊的穿透深度。
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公开(公告)号：US5281532A
公开(公告)日：1994-01-25
申请号：US980129
申请日：1992-11-23
IPC分类号： A01N63/00 , A01N63/04 , C07K14/325 , C12P1/04 , C12N1/21
CPC分类号： C07K14/325 , A01N63/00 , A01N63/04 , C12P1/04
摘要： Novel biological pesticides are prepared by introducing into a microorganism the genetic capability to produce a heterologous pesticide, wherein the microorganism is capable of proliferating in the rhizosphere or phylloplane in competition with wild-type microorganisms. A gene capable of expressing a polypeptide is introduced into the microorganism under conditions which allow for stable maintenance and expression of the gene, without significantly diminishing the ability of the microorganism to compete in the environment. Preferred microorganisms provide for the maintenance and protection from degradation of the polypeptide pesticide.
摘要翻译： 通过向微生物中引入产生异源农药的遗传能力来制备新型生物农药，其中微生物能够在与野生型微生物竞争的根际或叶面中增殖。 能够表达多肽的基因在允许基因的稳定维持和表达的条件下引入微生物，而不显着降低微生物在环境中竞争的能力。 优选的微生物提供维持和保护以免多肽农药的降解。
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公开(公告)号：US5333613A
公开(公告)日：1994-08-02
申请号：US035466
申请日：1993-03-23
CPC分类号： A61K49/223
摘要： Ultrasonic contrast media are provided comprising small particles with entrapped gas, where the compositions can be selected for rapid dissolution with production of microbubbles or maintaining the gas in microtubules, microchambers or microcracks, providing for echogenicity. The particles are readily formed by precipitation from a liquid, either solution or melt, of a monomeric material, followed by drying and mechanically reducing the size of the particles.
摘要翻译： 提供超声波造影剂，其包含具有截留气体的小颗粒，其中可以选择组合物用于快速溶解以产生微泡或将气体保持在微管，微泡或微裂纹中，从而提供回声性。 通过从单体材料的液体，溶液或熔体中沉淀，然后干燥并机械地减小颗粒的尺寸，容易形成颗粒。
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公开(公告)号：US5327891A
公开(公告)日：1994-07-12
申请号：US922617
申请日：1992-07-30
申请人： David H. Rammler
发明人： David H. Rammler
CPC分类号： A61B8/0833 , A61B8/0841 , A61B8/481 , A61B2090/3925 , A61M2025/0034 , A61M2025/0037 , A61M2025/0039
摘要： Catheters are provided having a plurality of vanes external to a central core serving as channels for microbubbles. A track may be provided having grooves in which one of the vanes passes, which track provides additional microbubbles, so as to allow for following the movement of the catheter and track by ultrasonic imaging. The catheter provides for reduced abrasion, as well as ease of following the movement of the catheter through the patient's vessels.
摘要翻译： 提供导管，其具有作为用于微泡的通道的中心核心外部的多个叶片。 可以设置具有凹槽的轨道，其中一个叶片通过，该轨道提供额外的微泡，以允许通过超声成像跟随导管和轨迹的运动。 导管提供减少的磨损，以及易于跟随导管穿过患者血管的运动。
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公开(公告)号：US5217591A
公开(公告)日：1993-06-08
申请号：US954911
申请日：1992-09-30
申请人： Erich Gombocz , Mark Dwight , David H. Rammler
发明人： Erich Gombocz , Mark Dwight , David H. Rammler
IPC分类号： G01N27/447
CPC分类号： G01N27/44704 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： A sample applicator/retriever for gel electrophoresis is provided, which allows easy and reproducible application or retrieval of samples. The sample nozzle is designed to load samples uniformly through a gel. The applicator/retriever may be in several different forms, including a a multiwell device, individual wells and mounting bar, a single line well, or a concentrator applicator.
摘要翻译： 提供了用于凝胶电泳的样品施加器/回收器，其允许容易且可再现的样品的应用或检索。 样品喷嘴设计为通过凝胶均匀地加载样品。 施放器/回收器可以是几种不同的形式，包括多孔装置，单独的井和安装杆，单线井或集中器施加器。
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