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    Capture probe-based library normalization
    1.
    发明授权
    Capture probe-based library normalization 有权

    公开(公告)号：US12071654B2

    公开(公告)日：2024-08-27

    申请号：US16837425

    申请日：2020-04-01

    申请人： Sequenom, Inc.

    发明人： Tricia Zwiefelhofer ,  Jason Nathanson

    IPC分类号： C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06

    CPC分类号： C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C12Q2600/158

    摘要： Provided herein are methods of normalizing nucleic acid libraries. The method uses nucleic acid probes with nucleic acid sequences that are complementary to one or more of these adaptor sequences are added to the nucleic acids libraries. The probes can hybridize to the adaptor sequences in the single stranded nucleic acid molecules derived from the libraries to form hybridization complexes. The probes are conjugated to a first binding member, which can interact with a second binding member that is conjugated to solid supports. The solid supports can then be collected and the single stranded nucleic acid molecules can be recovered in a volume of elution buffer to reach a desired concentration. As compared to standard methods, the methods are more efficient and cost-effective.

    NON-INVASIVE DETECTION OF FETAL GENETIC TRAITS
    2.
    发明公开
    NON-INVASIVE DETECTION OF FETAL GENETIC TRAITS 审中-公开

    公开(公告)号：US20240182970A1

    公开(公告)日：2024-06-06

    申请号：US18538488

    申请日：2023-12-13

    申请人： SEQUENOM, INC.

    发明人： Sinuhe HAHN ,  Wolfgang HOLZGREVE ,  Bernhard ZIMMERMANN ,  Ying LI

    IPC分类号： C12Q1/6883 ,  C12Q1/6806

    CPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12Q2600/156

    摘要： Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ≤500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of ≤500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ≤500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.

    Sequencing Adapter Manufacture and Use
    6.
    发明申请
    Sequencing Adapter Manufacture and Use 有权

    公开(公告)号：US20230135846A1

    公开(公告)日：2023-05-04

    申请号：US17751017

    申请日：2022-05-23

    申请人： Sequenom, Inc.

    发明人： Taylor Jensen ,  Christopher Ellison

    IPC分类号： C12Q1/6855 ,  C12N15/10 ,  C12Q1/6869 ,  G16B30/00

    摘要： Technology provided herein relates in part to methods, processes, machines and apparatuses for determining sequences of nucleotides for nucleic acid templates in a nucleic acid sample. The technology provide herein also relates in part to methods, processes, machines and apparatuses for counting nucleic acid templates. Nucleic acid templates of a sample are tagged with nonrandom oligonucleotide adapters that include predetermined non-randomly generated sequences. The use of these nonrandom oligonucleotide adapters provides an efficient method to reduce sequencing errors, and increase the sensitivity of detection of low-frequency single nucleotide alterations.