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公开(公告)号:US20230408491A1
公开(公告)日:2023-12-21
申请号:US18083201
申请日:2022-12-16
Inventor: Shaun David MCCULLOUGH , Alysha SIMMONS
CPC classification number: G01N33/5044 , C12N5/0688 , G01N33/582 , G01N21/6428 , G01N2021/6439
Abstract: A method of testing a test substance in an in vitro model of a human tracheobronchial respiratory tract, includes: providing the in vitro model including a cell culture including airway epithelial cells (AECs), a basolateral compartment below the AECs and an apical compartment above the AECs, wherein the AECs form a barrier between the basolateral AND apical compartments; adding the test substance to the apical and/or basolateral compartment; adding a tracer to the basolateral compartment, which is fluorescent, has a molecular weight within 5 kDa of human albumin and is added before, during or after adding the test substance; incubating the cell culture system in a presence of the tracer; collecting at least one sample from the apical compartment; and measuring a fluorescence thereof to determine an effect of the test substance on the AECs. A kit is also disclosed.
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公开(公告)号:US11787706B2
公开(公告)日:2023-10-17
申请号:US17386228
申请日:2021-07-27
Applicant: U.S. Environmental Protection Agency
Inventor: Hodon Ryu , Hyoungmin Woo
CPC classification number: C02F1/325 , A61L2/088 , A61L2/10 , A61L2/26 , C02F1/725 , A61L2202/11 , A61L2202/122 , C02F2201/3222 , C02F2201/3227 , C02F2201/3228 , C02F2303/04 , C02F2305/10
Abstract: A point-of-use (POU) water filtration device has a container. A plurality of channels is formed within the container, water entering the container flowing through the plurality of channels. A plurality of Ultraviolet (UV) Light Emitting Diodes (LEDs) is provided. Each of the plurality of UV LEDs illuminating UV light down an associated channel of the plurality of channels.
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公开(公告)号:US11320411B2
公开(公告)日:2022-05-03
申请号:US16288925
申请日:2019-02-28
Inventor: Paul A. Solomon , Thomas Kirchstetter
Abstract: The invention relates to a device for measuring, in near-real-time, the level of black carbon, brown carbon, organic carbon, total carbon and CO2 in air. The device also provides for a direct calculation of aerosol angstrom coefficient as well as estimation of emissions rates of black carbon or brown carbon from nearby combustion sources.
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公开(公告)号:US11255783B2
公开(公告)日:2022-02-22
申请号:US16556726
申请日:2019-08-30
Applicant: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS , GARVER, LLC , THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE ADMINISTRATOR OF U.S. ENVIRONMENTAL PROTECTION AGENCY
Inventor: Julian Fairey , Thien Duc Do , Ashley Pifer , David Wahman
Abstract: This invention relates generally to a system and process for early detection of biological ammonia oxidation in water utilizing a fluorescence-based sensor and process. Various embodiments are configured to read increases in a fluorescence excitation-emission wavelength pair that is responsive to a period of time (days to weeks or even longer) prior to the onset of biological ammonia oxidation, which is considered to be a nitrification event. Fluorescence excitation/emission pairs that have proven to be reliable include a fluorescence excitation wavelength of about 230 nm and an emission wavelength of about 345 nm and an excitation wavelength of 325 and an emission wavelength of 470. The system and process enable drinking water utilities to improve management of its distribution systems and facilitate earlier corrective actions, resulting is less loss of treated water through flushing and other tangible benefits.
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公开(公告)号:US20210371304A1
公开(公告)日:2021-12-02
申请号:US17386228
申请日:2021-07-27
Applicant: U.S. Environmental Protection Agency
Inventor: HODON RYU , HYOUNGMIN WOO
Abstract: A point-of-use (POU) water filtration device has a container. A plurality of channels is formed within the container, water entering the container flowing through the plurality of channels. A plurality of Ultraviolet (UV) Light Emitting Diodes (LEDs) is provided. Each of the plurality of UV LEDs illuminating UV light down an associated channel of the plurality of channels.
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公开(公告)号:US11060896B2
公开(公告)日:2021-07-13
申请号:US16690475
申请日:2019-11-21
Applicant: GOVERNMENT OF THE UNITED STATES AS REPRESENTED BY THE ADMINISTRATOR OF THE U.S. ENVIRONMENTAL PROTECTION AGENCY , URBANALTA CORP
Inventor: Ruben Kertesz , Daniel J. Murray, Jr. , Michael Bolan , Ryan Anderson , Chunsheng Fang
Abstract: A flow imaging and monitoring system for synchronized management of wide area drainage that includes an interposer for supporting monitoring and management equipment in a manhole, a module for illuminating water flowing in pipes at the base of the manhole, a module for monitoring responses to reflected light, a sealed and rechargeable battery pack, and a data analysis and management system to interpret data streams in real time. The interposer can be adjusted to fit the diameter of the manhole and can be adjusted to be placed under the manhole cover. The module for illuminating the flowing water can be adjusted to generate various frequencies. The support structures for the modules can be adjusted for varying pitch, roll and yaw with respect to the manhole. The data analysis and management system is supported by cloud computing.
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公开(公告)号:US20200094201A1
公开(公告)日:2020-03-26
申请号:US15379905
申请日:2016-12-15
Applicant: U.S. Environmental Protection Agency
Inventor: Christopher Ruybal , Dominic DiGiulio
Abstract: A rapid and continuous separator or equilibrator to separate a gas from a liquid includes a venturi and injector, a mixer and a free overfall stream to separate a gas from a liquid. The injector introduces a carrier medium into the liquid which provides a reservoir for the gas to diffuse into as the liquid and carrier make a single transit through the apparatus. The separator was developed to enable real-time estimation of methane concentrations in ground water during purging. Real-time monitoring allows evaluation of trends during water well purging, spatial trends between water wells, and temporal comparisons between sampling events. These trends may be a result of removal of stored casing water, pre-purge ambient borehole flow, formation physical and chemical heterogeneity, or vertical flow outside of well casing due to poor bentonite or cement seals. Real-time information in the field can help focus an investigation, aid in determining when to collect a sample, save money by limiting costs (e.g. analytical, sample transport and storage), and provide an immediate assessment of local methane concentrations, Four domestic water wells, one municipal water well, and one agricultural water well were sampled for traditional laboratory analysis and compared to the field separator or equilibrator results. Applying a paired t-test comparing the new separator or equilibrator method and traditional laboratory analysis yielded a p-value 0.383, suggesting no significant difference between the two methods for the current study. Additional field and laboratory-based experimentation and potential modification of this device are necessary to justify use beyond screening at this time. However, early separator or equilibrator use suggests promising results and applications.
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公开(公告)号:US20200017375A1
公开(公告)日:2020-01-16
申请号:US16520880
申请日:2019-07-24
Applicant: U.S. Environmental Protection Agency
Inventor: HODON RYU , HYOUNGMIN WOO
Abstract: A point-of-use (POU) water filtration device has a container. A plurality of channels is formed within the container, water entering the container flowing through the plurality of channels. A plurality of Ultraviolet (UV) Light Emitting Diodes (LEDs) is provided. Each of the plurality of UV LEDs illuminating UV light down an associated channel of the plurality of channels.
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公开(公告)号:US10290089B2
公开(公告)日:2019-05-14
申请号:US14545574
申请日:2015-05-22
Inventor: Blake Allen Schaeffer , Robyn Nicole Conmy , Darryl Joel Keith , Ross S. Lunetta , Mark S. Murphy , Torrin Hultgren
IPC: G06K9/00 , G06T7/00 , H04W4/02 , G06F16/9537 , H04L29/08
Abstract: Systems and methods are used to determine the location and severity of harmful algal blooms or other water quality parameters. GPS location information is transmitted from a mobile device and recent Satellite image data and water quality parameters are provided to the mobile device. Data regarding other locations, historical water quality parameters and algorithm based predictive results are provided for the end user. This provides for near-real time information allowing users to make decisions regarding fishing, beach closures, municipal water intake, etc. so as to avoid toxic effects of a harmful algal bloom.
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公开(公告)号:US10245558B2
公开(公告)日:2019-04-02
申请号:US15183115
申请日:2016-06-15
Inventor: Dibakar Bhattacharyya , Vasile Smuleac , Rajender S. Varma , Subhas K. Sikdar
IPC: B01D67/00 , B01D69/14 , B01D71/02 , B01D71/34 , C02F1/44 , B01D69/12 , B01D69/10 , B01D71/40 , C02F1/70 , C02F101/36 , C02F103/06 , B01D71/44 , B82Y30/00 , B82Y40/00 , C02F1/00
Abstract: A nanocomposite membrane includes a macroporous polymer membrane having a plurality of pores. A plurality of metal nanoparticles are synthesized and immobilized within those plurality of pores. The nanoparticles are reduced and capped with a green reducing and capping agent such as green tea extract.
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