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公开(公告)号：US07687611B2
公开(公告)日：2010-03-30
申请号：US11119631
申请日：2005-05-02
IPC分类号： A23J1/00 , A61K39/205 , A61K39/38 , A61K39/145
CPC分类号： C07K14/005 , A61K39/00 , C12N7/00 , C12N2760/16122 , C12N2760/16151 , C12N2760/16222 , C12N2760/16251 , C12N2760/16322 , C12N2760/16351 , G01N33/68 , G01N33/6842 , G01N2333/11 , G01N2333/12
摘要： The present invention relates to novel methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
摘要翻译： 本发明涉及用于分离血凝素（HA）抗原的新方法，其包括以下步骤：在反相高效液相色谱柱上将包含溶解的HA抗原和洗涤剂的还原和衍生抗原制剂施用于pH控制溶液中 ; 并用离子对试剂在有机流动相中从柱中洗脱HA抗原。 本发明还涉及使用分离抗原的方法的定量方法，其中进一步的步骤是在洗脱步骤得到的色谱图中测定洗脱抗原的峰面积。
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2.发明申请公开(公告)号：US20060051742A1
公开(公告)日：2006-03-09
申请号：US11119631
申请日：2005-05-02
申请人： Johan Kapteyn , Fija Lagerwerf
发明人： Johan Kapteyn , Fija Lagerwerf
CPC分类号： C07K14/005 , A61K39/00 , C12N7/00 , C12N2760/16122 , C12N2760/16151 , C12N2760/16222 , C12N2760/16251 , C12N2760/16322 , C12N2760/16351 , G01N33/68 , G01N33/6842 , G01N2333/11 , G01N2333/12
摘要： The present invention relates to novel methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
摘要翻译： 本发明涉及用于分离血凝素（HA）抗原的新方法，其包括以下步骤：在反相高效液相色谱柱上将包含溶解的HA抗原和洗涤剂的还原和衍生抗原制剂施用于pH控制溶液中 ; 并用离子对试剂在有机流动相中从柱中洗脱HA抗原。 本发明还涉及使用分离抗原的方法的定量方法，其中进一步的步骤是在洗脱步骤得到的色谱图中测定洗脱抗原的峰面积。
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3.发明申请公开(公告)号：US20110097785A1
公开(公告)日：2011-04-28
申请号：US12999105
申请日：2009-06-09
申请人： Monty R. Warthen , William Skotta
发明人： Monty R. Warthen , William Skotta
CPC分类号： C12N7/00 , C12N2760/16151 , C12N2760/16152 , C12N2760/16251 , C12N2760/16351
摘要： Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process.
摘要翻译： 描述了可以被病毒感染并且适于在不含动物衍生组分的培养基（例如无血清培养基）中在悬浮液中生长的动物细胞。 此外还描述了使用这些细胞在细胞培养中复制病毒的方法，以及含有可通过该方法获得的病毒或其抗原部分的疫苗。
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公开(公告)号：US4117113A
公开(公告)日：1978-09-26
申请号：US826409
申请日：1977-08-22
IPC分类号： A61K9/127 , A61K39/12 , A61K39/145 , A61K39/39 , A61K39/18
CPC分类号： A61K39/145 , A61K39/12 , A61K39/39 , A61K9/127 , C12N7/00 , A61K2039/55555 , A61K2039/55572 , C12N2760/16134 , C12N2760/16151 , C12N2760/16251 , C12N2760/16351 , Y10S424/812
摘要： Immunological preparations are described in which liposomes having negative charge are used as adjuvants for the purposes of human and veterinary vaccines containing viral antigens. The formulation of influenza antigen in liposomes formed with egg lecithin is described and the adjuvant effect demonstrated.
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公开(公告)号：US20180339036A1
公开(公告)日：2018-11-29
申请号：US15031329
申请日：2014-10-24
申请人： LEUKOCARE AG
CPC分类号： A61K39/145 , A61K9/08 , A61K39/12 , A61K47/183 , A61K47/26 , A61K47/36 , A61P31/16 , C12N2760/16134 , C12N2760/16151 , C12N2760/16251 , C12N2760/16351
摘要： The present invention relates to a method for producing stabilised vaccines, the method comprising: (a) mixing antigens with a solution comprising: (i) chitosan; (ii) at least three different amino acids and/or at least one dipeptide or tripeptide; and (iii) a sugar; and (b) drying the mixture obtained in (a).
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公开(公告)号：US20150322411A1
公开(公告)日：2015-11-12
申请号：US14802087
申请日：2015-07-17
申请人： Intervet Inc.
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N2760/16151 , C12N2760/16152 , C12N2760/16251 , C12N2760/16351
摘要： Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process.
摘要翻译： 描述了可以被病毒感染并且适于在不含动物衍生组分的培养基（例如无血清培养基）中在悬浮液中生长的动物细胞。 此外还描述了使用这些细胞在细胞培养中复制病毒的方法，以及含有可通过该方法获得的病毒或其抗原部分的疫苗。
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7.发明申请公开(公告)号：US20100041022A1
公开(公告)日：2010-02-18
申请号：US12587136
申请日：2009-10-02
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , A61K39/00 , C12N7/00 , C12N2760/16122 , C12N2760/16151 , C12N2760/16222 , C12N2760/16251 , C12N2760/16322 , C12N2760/16351 , G01N33/68 , G01N33/6842 , G01N2333/11 , G01N2333/12
摘要： Described are methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
摘要翻译： 描述了分离血凝素（HA）抗原的方法，包括以下步骤：在反相高效液相色谱（RP-HPLC）上，将包含溶解的HA抗原和洗涤剂的还原的和衍生的抗原制剂施用于pH控制的溶液中， 柱; 并用离子对试剂在有机流动相中从柱中洗脱HA抗原。 本发明还涉及使用分离抗原的方法的定量方法，其中进一步的步骤是在洗脱步骤得到的色谱图中测定洗脱抗原的峰面积。
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8.发明申请公开(公告)号：US20070196387A1
公开(公告)日：2007-08-23
申请号：US10592743
申请日：2005-03-03
申请人： Johan Kapteyn , Fija Lagerwerf
发明人： Johan Kapteyn , Fija Lagerwerf
IPC分类号： A61K39/145 , C07K14/11
CPC分类号： C07K14/005 , A61K39/00 , C12N7/00 , C12N2760/16122 , C12N2760/16151 , C12N2760/16222 , C12N2760/16251 , C12N2760/16322 , C12N2760/16351 , G01N33/68 , G01N33/6842 , G01N2333/11 , G01N2333/12
摘要： The present invention relates to novel methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
摘要翻译： 本发明涉及用于分离血凝素（HA）抗原的新方法，包括以下步骤：将反应型高效液相色谱法（pH值控制溶液中的溶解的HA抗原和洗涤剂）的还原和衍生抗原制剂 RP-HPLC）柱; 并用离子对试剂在有机流动相中从柱中洗脱HA抗原。 本发明还涉及使用分离抗原的方法的定量方法，其中进一步的步骤是在洗脱步骤得到的色谱图中测定洗脱抗原的峰面积。
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9.发明授权公开(公告)号：US09441207B2
公开(公告)日：2016-09-13
申请号：US14802087
申请日：2015-07-17
申请人： Intervet Inc.
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N2760/16151 , C12N2760/16152 , C12N2760/16251 , C12N2760/16351
摘要： Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process.
摘要翻译： 描述了可以被病毒感染并且适于在不含动物衍生组分的培养基（例如无血清培养基）中在悬浮液中生长的动物细胞。 此外还描述了使用这些细胞在细胞培养中复制病毒的方法，以及含有可通过该方法获得的病毒或其抗原部分的疫苗。
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公开(公告)号：US08283452B2
公开(公告)日：2012-10-09
申请号：US12587136
申请日：2009-10-02
IPC分类号： A23J1/00 , A61K39/205
CPC分类号： C07K14/005 , A61K39/00 , C12N7/00 , C12N2760/16122 , C12N2760/16151 , C12N2760/16222 , C12N2760/16251 , C12N2760/16322 , C12N2760/16351 , G01N33/68 , G01N33/6842 , G01N2333/11 , G01N2333/12
摘要： Described are methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
摘要翻译： 描述了分离血凝素（HA）抗原的方法，包括以下步骤：在反相高效液相色谱（RP-HPLC）上，将包含溶解的HA抗原和洗涤剂的还原的和衍生的抗原制剂施用于pH控制的溶液中， 柱; 并用离子对试剂在有机流动相中从柱中洗脱HA抗原。 本发明还涉及使用分离抗原的方法的定量方法，其中进一步的步骤是在洗脱步骤得到的色谱图中测定洗脱抗原的峰面积。
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