公开(公告)号：US20100041022A1

公开(公告)日：2010-02-18

申请号：US12587136

申请日：2009-10-02

申请人： Johan Christiaan Kapteyn ,  Fija Maria Lagerwerf

发明人： Johan Christiaan Kapteyn ,  Fija Maria Lagerwerf

IPC分类号： C12Q1/70

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N2760/16122 ,  C12N2760/16151 ,  C12N2760/16222 ,  C12N2760/16251 ,  C12N2760/16322 ,  C12N2760/16351 ,  G01N33/68 ,  G01N33/6842 ,  G01N2333/11 ,  G01N2333/12

摘要： Described are methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.

摘要翻译： 描述了分离血凝素（HA）抗原的方法，包括以下步骤：在反相高效液相色谱（RP-HPLC）上，将包含溶解的HA抗原和洗涤剂的还原的和衍生的抗原制剂施用于pH控制的溶液中， 柱; 并用离子对试剂在有机流动相中从柱中洗脱HA抗原。 本发明还涉及使用分离抗原的方法的定量方法，其中进一步的步骤是在洗脱步骤得到的色谱图中测定洗脱抗原的峰面积。

公开(公告)号：US07638608B2

公开(公告)日：2009-12-29

申请号：US10592743

申请日：2005-03-03

申请人： Johan Christiaan Kapteyn ,  Fija Maria Lagerwerf

发明人： Johan Christiaan Kapteyn ,  Fija Maria Lagerwerf

IPC分类号： A23J1/00 ,  C07K16/00 ,  A61K39/38 ,  A61K39/145 ,  A61K39/205

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N2760/16122 ,  C12N2760/16151 ,  C12N2760/16222 ,  C12N2760/16251 ,  C12N2760/16322 ,  C12N2760/16351 ,  G01N33/68 ,  G01N33/6842 ,  G01N2333/11 ,  G01N2333/12

摘要： The present invention relates to novel methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.

摘要翻译： 本发明涉及用于分离血凝素（HA）抗原的新方法，包括以下步骤：将反应型高效液相色谱法（pH值控制溶液中的溶解的HA抗原和洗涤剂）的还原和衍生抗原制剂 RP-HPLC）柱; 并用离子对试剂在有机流动相中从柱中洗脱HA抗原。 本发明还涉及使用分离抗原的方法的定量方法，其中进一步的步骤是在洗脱步骤得到的色谱图中测定洗脱抗原的峰面积。

公开(公告)号：US07304031B2

公开(公告)日：2007-12-04

申请号：US10494140

申请日：2002-10-29

申请人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

发明人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

IPC分类号： A61K38/00 ,  C07K14/505

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/505 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12P21/005

摘要： Described are methods for identifying, selecting, and obtaining mammalian cells capable of producing proteinaceous molecules having predetermined post-translational modifications, wherein the post-translational modifications are brought about by the mammalian cell in which the proteinaceous molecule is expressed. Preferably, the predetermined post-translational modifications include glycosylation. Also described are methods for obtaining and producing proteinaceous molecules, using mammalian cells obtainable by a method of the present invention. Preferably, the proteinaceous molecules includes erythropoietin (EPO), since EPO's effect depends heavily on its glycosylation pattern. Mammalian cells that have been obtained on the basis of their ability to produce proteins and/or post-translational modifications that are indicative for a predetermined post-translational modification that is desired are also provided. Preferably, the mammalian cells have neural characteristics and properties such that significant amounts of recombinant proteins can be produced that harbor ‘neural- or brain-type’ properties.

摘要翻译： 描述了用于鉴定，选择和获得能够产生具有预定的翻译后修饰的蛋白质分子的哺乳动物细胞的方法，其中翻译后修饰由其中表达蛋白质分子的哺乳动物细胞产生。 优选地，预定的翻译后修饰包括糖基化。 还描述了使用可通过本发明的方法获得的哺乳动物细胞获得和产生蛋白质分子的方法。 优选地，蛋白质分子包括促红细胞生成素（EPO），因为EPO的作用在很大程度上取决于其糖基化模式。 还提供了基于其产生蛋白质的能力获得的哺乳动物细胞和/或指示期望的预定的翻译后修饰的翻译后修饰。 优选地，哺乳动物细胞具有神经特征和性质，使得可以产生含有“神经或脑型”性质的显着量的重组蛋白。

公开(公告)号：US07785833B2

公开(公告)日：2010-08-31

申请号：US11657202

申请日：2007-01-24

申请人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianius Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

发明人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianius Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

IPC分类号： C12N5/00 ,  C12N15/00 ,  G01N33/53 ,  C07K14/475

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/505 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12P21/005

摘要： Described are methods for identifying, selecting, and obtaining mammalian cells capable of producing proteinaceous molecules having predetermined post-translational modifications, wherein the post-translational modifications are brought about by the mammalian cell in which the proteinaceous molecule is expressed. Preferably, the predetermined post-translational modifications include glycosylation. Also described are methods for obtaining and producing proteinaceous molecules, using mammalian cells obtainable by a method of the present invention. Preferably, the proteinaceous molecules include erythropoietin (EPO), since EPO's effect depends heavily on its glycosylation pattern. Mammalian cells that have been obtained on the basis of their ability to produce proteins and/or post-translational modifications that are indicative for a predetermined post-translational modification that is desired are also provided. Preferably, the mammalian cells have neural characteristics and properties such that significant amounts of recombinant proteins can be produced that harbor “neural- or brain-type” properties.

摘要翻译： 描述了用于鉴定，选择和获得能够产生具有预定的翻译后修饰的蛋白质分子的哺乳动物细胞的方法，其中翻译后修饰由其中表达蛋白质分子的哺乳动物细胞产生。 优选地，预定的翻译后修饰包括糖基化。 还描述了使用可通过本发明的方法获得的哺乳动物细胞获得和产生蛋白质分子的方法。 优选地，蛋白质分子包括促红细胞生成素（EPO），因为EPO的作用很大程度上取决于其糖基化模式。 还提供了基于其产生蛋白质的能力获得的哺乳动物细胞和/或指示期望的预定的翻译后修饰的翻译后修饰。 优选地，哺乳动物细胞具有神经特征和性质，使得可以产生具有“神经或脑型”特性的显着量的重组蛋白。

公开(公告)号：US07696157B2

公开(公告)日：2010-04-13

申请号：US11888776

申请日：2007-08-01

申请人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

发明人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

IPC分类号： A61K38/18 ,  C07K14/505

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/505 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12P21/005

摘要： Described are methods for identifying, selecting, and obtaining mammalian cells capable of producing proteinaceous molecules having predetermined post-translational modifications, wherein the post-translational modifications are brought about by the mammalian cell in which the proteinaceous molecule is expressed. Preferably, the predetermined post-translational modifications include glycosylation. Also described are methods for obtaining and producing proteinaceous molecules, using mammalian cells obtainable by a method of the present invention. Preferably, the proteinaceous molecules include erythropoietin (EPO), since EPO's effect depends heavily on its glycosylation pattern. Mammalian cells that have been obtained on the basis of their ability to produce proteins and/or post-translational modifications that are indicative for a predetermined post-translational modification that is desired are also provided. Preferably, the mammalian cells have neural characteristics and properties, such that significant amounts of recombinant proteins can be produced that harbor “neural- or brain-type” properties.

摘要翻译： 描述了用于鉴定，选择和获得能够产生具有预定的翻译后修饰的蛋白质分子的哺乳动物细胞的方法，其中翻译后修饰由其中表达蛋白质分子的哺乳动物细胞产生。 优选地，预定的翻译后修饰包括糖基化。 还描述了使用可通过本发明的方法获得的哺乳动物细胞获得和产生蛋白质分子的方法。 优选地，蛋白质分子包括促红细胞生成素（EPO），因为EPO的作用很大程度上取决于其糖基化模式。 还提供了基于其产生蛋白质的能力获得的哺乳动物细胞和/或指示期望的预定的翻译后修饰的翻译后修饰。 优选地，哺乳动物细胞具有神经特征和性质，使得可以产生含有“神经或脑型”性质的显着量的重组蛋白。