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    Method for nucleic acid amplification that results in low amplification bias
    1.
    发明授权
    Method for nucleic acid amplification that results in low amplification bias 有权
    导致低扩增偏倚的核酸扩增方法

    公开(公告)号：US07297485B2

    公开(公告)日：2007-11-20

    申请号：US10429229

    申请日：2003-05-02

    申请人： Carole Bornarth ,  Michele Wisniewski ,  Seiyu Hosono ,  Arumugham Raghunathan ,  Roger S. Lasken

    发明人： Carole Bornarth ,  Michele Wisniewski ,  Seiyu Hosono ,  Arumugham Raghunathan ,  Roger S. Lasken

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6844 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2531/119 ,  C12Q2523/119 ,  C12Q2523/113 ,  C12Q2527/137 ,  C12Q2527/119 ,  C12Q2537/143 ,  C12Q2525/125

    摘要： Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.

    摘要翻译： 公开了用于扩增感兴趣的核酸序列的组合物和方法。 已经发现，如果对阈值量以上或超过阈值量的核酸量进行扩增反应和/或在阈值浓度以下或阈值浓度的核酸上，扩增反应可以产生高质量的扩增产物，例如低扩增偏差。 阈值和浓度可以根据待扩增的核酸的性质和来源以及所采用的扩增反应的类型而变化。 公开了一种确定可以与感兴趣的扩增反应中感兴趣的核酸样品一起使用的核酸的阈值量和/或阈值浓度的方法。 因为扩增反应可以产生高质量的扩增产物，例如低偏差扩增产物，低于阈值量和/或核酸浓度，这种低于阈值的量和/或浓度可用于扩增反应。

    Method
    3.
    发明申请
    Method 审中-公开
    方法

    公开(公告)号：US20050244827A1

    公开(公告)日：2005-11-03

    申请号：US10510107

    申请日：2003-04-04

    申请人： Charlotta Olsson ,  Nigel Tooke

    发明人： Charlotta Olsson ,  Nigel Tooke

    IPC分类号： G01N21/78 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2563/107 ,  C12Q2527/137 ,  C12Q2523/107 ,  C12Q2533/101 ,  C12Q2523/119

    摘要： A method for determining the sequence of a nucleic acid molecule includes the steps of: a) providing a single-stranded form of the nucleic acid molecule; b) hybridizing a primer to the single stranded form of the nucleic acid molecule to form a template/primer complex; c) enzymatically extending the primer by the addition of a polymerase and a mixture of at least one nucleotide and at least one labeled derivative of the at least one nucleotide, wherein the at least one labeled derivative of the at least one nucleotide includes a label linked to the nucleotide via a cleavable link and wherein the amount of labeled derivative of the at least one nucleotide in the mixture of the at least one nucleotide and the labeled derivative of the at least one nucleotide is within the range of 1-50 mole-%, 1-40 mole-%, 1-30 mole-%, or 1-20 mole-%. d) determining the type of nucleotide added to the primer; and e) repeating steps c) to d) at least once.

    摘要翻译： 确定核酸分子序列的方法包括以下步骤：a）提供核酸分子的单链形式; b）将引物与核酸分子的单链形式杂交以形成模板/引物复合物; c）通过加入聚合酶和所述至少一个核苷酸的至少一个核苷酸和至少一个标记的衍生物的混合物酶促延伸引物，其中至少一个核苷酸的至少一个标记的衍生物包括连接的标记 通过可切割链接与核苷酸反应，并且其中至少一个核苷酸和至少一个核苷酸的标记衍生物的混合物中的至少一个核苷酸的标记的衍生物的量在1-50摩尔％ ，1-40摩尔％，1-30摩尔％，或1-20摩尔％。 d）确定添加到引物中的核苷酸类型; 和e）重复步骤c）至d）至少一次。

    NUCLEIC ACID AMPLIFICATION
    6.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开
    核酸放大

    公开(公告)号：US20080128298A1

    公开(公告)日：2008-06-05

    申请号：US11871707

    申请日：2007-10-12

    申请人： Carole Bornarth ,  Michele Wisniewski ,  Seiyu Hosono ,  Arumugham Raghunathan ,  Roger S. Lasken

    发明人： Carole Bornarth ,  Michele Wisniewski ,  Seiyu Hosono ,  Arumugham Raghunathan ,  Roger S. Lasken

    IPC分类号： B65D69/00 ,  C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2531/119 ,  C12Q2523/119 ,  C12Q2523/113 ,  C12Q2527/137 ,  C12Q2527/119 ,  C12Q2537/143 ,  C12Q2525/125

    摘要： Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.

    摘要翻译： 公开了用于扩增感兴趣的核酸序列的组合物和方法。 已经发现，如果对阈值量以上或超过阈值量的核酸量进行扩增反应，和/或在阈值浓度或低于阈值浓度的核酸上，扩增反应可以产生高质量的扩增产物，例如低扩增偏差。 阈值和浓度可以根据待扩增的核酸的性质和来源以及所采用的扩增反应的类型而变化。 公开了一种确定可以与感兴趣的扩增反应中感兴趣的核酸样品一起使用的核酸的阈值量和/或阈值浓度的方法。 因为扩增反应可以产生高质量的扩增产物，例如低偏差扩增产物，低于阈值量和/或核酸浓度，这种低于阈值的量和/或浓度可用于扩增反应。

    Nucleic acid amplification
    10.
    发明申请
    Nucleic acid amplification 有权
    核酸扩增

    公开(公告)号：US20030228613A1

    公开(公告)日：2003-12-11

    申请号：US10429229

    申请日：2003-05-02

    发明人： Carole Bornarth ,  Michele Wisniewski ,  Seiyu Hosono ,  Arumugham Raghunathan ,  Roger S. Lasken

    IPC分类号： C12Q001/68 ,  C12P019/34

    CPC分类号： C12Q1/6844 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2531/119 ,  C12Q2523/119 ,  C12Q2523/113 ,  C12Q2527/137 ,  C12Q2527/119 ,  C12Q2537/143 ,  C12Q2525/125

    摘要： Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.

    摘要翻译： 公开了用于扩增感兴趣的核酸序列的组合物和方法。 已经发现，如果对阈值量以上或超过阈值量的核酸量进行扩增反应和/或在阈值浓度以下或阈值浓度的核酸上，扩增反应可以产生高质量的扩增产物，例如低扩增偏差。 阈值和浓度可以根据待扩增的核酸的性质和来源以及所采用的扩增反应的类型而变化。 公开了一种确定可以与感兴趣的扩增反应中感兴趣的核酸样品一起使用的核酸的阈值量和/或阈值浓度的方法。 因为扩增反应可以产生高质量的扩增产物，例如低偏差扩增产物，低于阈值量和/或核酸浓度，这种低于阈值的量和/或浓度可用于扩增反应。