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公开(公告)号：US20030186402A1
公开(公告)日：2003-10-02
申请号：US10257821
申请日：2003-03-10
IPC分类号： C12P007/06 , C12N001/18 , C12N001/16 , C12N015/74
CPC分类号： C12N9/0006 , C12Y101/0101 , C12Y101/01012 , Y02E50/17
摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.
摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。
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2.发明授权Mutant L-arabitol dehydrogenase derived from neurospora crassa with enhanced activity 有权来源于粗糙链孢菌的突变型L-阿拉伯糖醇脱氢酶具有增强的活性公开(公告)号：US09303278B2
公开(公告)日：2016-04-05
申请号：US14364631
申请日：2013-04-18
发明人： Jung Kul Lee , Hee Jung Moon , Manish Kumar Tiwari , Tae Su Kim
CPC分类号： C12P19/02 , C12N9/0006 , C12Y101/01012
摘要： The present invention is to enhance the stability and enzyme activity of an L-arabitol dehydrogenase derived from Neurospora crassa using techniques from quantum mechanics and molecular mechanics and partial mutation techniques. More specifically, the present invention relates to a method for preparing an L-arabitol dehydrogenase in which a residue which affects enzyme stability is found through a screening based on quantum mechanics and molecular mechanics, and enzyme activity is enhanced by mutation of the found residue, nucleic acid molecules encoding the L-arabitol dehydrogenase, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the L-arabitol dehydrogenase and an improved L-arabitol dehydrogenase.
摘要翻译： 本发明是利用来自量子力学和分子力学以及部分突变技术的技术提高源自粗糙脉孢菌的L-阿拉伯糖醇脱氢酶的稳定性和酶活性。 更具体地说，本发明涉及一种制备L-阿拉伯糖醇脱氢酶的方法，其中通过基于量子力学和分子力学的筛选发现影响酶稳定性的残基，并且酶活性通过发现的残基的突变增强， 编码L-阿拉伯糖醇脱氢酶的核酸分子，包含核酸分子的载体，包含载体的转化体，L-阿拉伯糖醇脱氢酶的突变体和改良的L-阿拉伯糖醇脱氢酶。
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公开(公告)号：US20090209016A1
公开(公告)日：2009-08-20
申请号：US12368509
申请日：2009-02-10
CPC分类号： C12N9/0006 , C12Y101/0101 , C12Y101/01012 , Y02E50/17
摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.
摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。
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4.发明申请NOVEL MUTANT L-ARABITOL DEHYDROGENASE DERIVED FROM NEUROSPORA CRASSA WITH ENHANCED ACTIVITY 有权从神经刺激素衍生的新型突变型L-阿拉伯糖脱氢酶与增强活性公开(公告)号：US20140370554A1
公开(公告)日：2014-12-18
申请号：US14364631
申请日：2013-04-18
发明人： Jung Kul Lee , Hee Jung Moon , Manish Kumar Tiwari , Tae Su Kim
CPC分类号： C12P19/02 , C12N9/0006 , C12Y101/01012
摘要： The present invention is to enhance the stability and enzyme activity of an L-arabitol dehydrogenase derived from Neurospora crassa using techniques from quantum mechanics and molecular mechanics and partial mutation techniques. More specifically, the present invention relates to a method for preparing an L-arabitol dehydrogenase in which a residue which affects enzyme stability is found through a screening based on quantum mechanics and molecular mechanics, and enzyme activity is enhanced by mutation of the found residue, nucleic acid molecules encoding the L-arabitol dehydrogenase, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the L-arabitol dehydrogenase and an improved L-arabitol dehydrogenase.
摘要翻译： 本发明是利用来自量子力学和分子力学以及部分突变技术的技术提高源自粗糙脉孢菌的L-阿拉伯糖醇脱氢酶的稳定性和酶活性。 更具体地说，本发明涉及一种制备L-阿拉伯糖醇脱氢酶的方法，其中通过基于量子力学和分子力学的筛选发现影响酶稳定性的残基，并且酶活性通过发现的残基的突变增强， 编码L-阿拉伯糖醇脱氢酶的核酸分子，包含核酸分子的载体，包含载体的转化体，L-阿拉伯糖醇脱氢酶的突变体和改良的L-阿拉伯糖醇脱氢酶。
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公开(公告)号：US07527951B2
公开(公告)日：2009-05-05
申请号：US10257821
申请日：2002-02-15
CPC分类号： C12N9/0006 , C12Y101/0101 , C12Y101/01012 , Y02E50/17
摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.
摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。
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