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    Method for production of C30-aldehyde carotenoids
    1.
    发明申请
    Method for production of C30-aldehyde carotenoids 失效
    生产C30-醛类胡萝卜素的方法

    公开(公告)号：US20040268436A1

    公开(公告)日：2004-12-30

    申请号：US10860291

    申请日：2004-06-03

    Inventor： Qiong Cheng ,  Luan Tao

    IPC: C12N015/82 ,  C12N015/87 ,  C12N015/74 ,  A01H005/00

    CPC classification number: C12N9/0083 ,  C12N9/1085 ,  C12N15/52 ,  C12P23/00

    Abstract: The present invention provides methods to engineer microorganisms for the production of C30-aldehyde carotenoids. Specifically, various combinations of crtM, sqs, crtN and crtN2 genes from Staphylococcus aureus and Methylomonas sp. 16a can be co-expressed in transformant hosts, leading to the production of diaponeurosporene monoaldehyde, diapocarotene monoaldehyde, and/or diapocarotene dialdehyde. In a preferred embodiment, the genetically engineered pathway is introduced into a strain of Escherichia coli that has been engineered for the expression of carotenoids, and the C30-carotenoid product is diapocarotene dialdehyde.

    Abstract translation: 本发明提供了用于生产C30-醛类胡萝卜素的微生物工程的方法。 具体来说，来自金黄色葡萄球菌和Methylomonas sp。的crtM，sqs，crtN和crtN2基因的各种组合。 16a可以在转化体宿主中共表达，导致产生二头孢噻吩一醛，二卡非罗芬单醛和/或二卡必康二醛。 在优选的实施方案中，将经遗传工程化的途径引入已被工程化用于表达类胡萝卜素的大肠杆菌菌株中，C30-类胡萝卜素产物是二卡必康二醛。

    Beta 1,4-N-acetylgalactosaminyltransferases form C. jejuni
    2.
    发明申请
    Beta 1,4-N-acetylgalactosaminyltransferases form C. jejuni 有权
    β-1,4- -N-乙酰半乳糖胺基转移酶形成空肠弯曲杆菌

    公开(公告)号：US20040265875A1

    公开(公告)日：2004-12-30

    申请号：US10830825

    申请日：2004-04-24

    Applicant: National Research Council of Canada

    Inventor： Michel Gilbert ,  Warren W. Wakarchuk

    IPC: C12Q001/68 ,  C07H021/04 ,  C12N009/10 ,  C08B037/00 ,  C12N015/74

    CPC classification number: C12N9/1241 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18

    Abstract: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an null2,3- and an null2,8-activity. A null1,4-GalNAc transferase and a null1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

    Abstract translation: 本发明提供了原核糖基转移酶，包括具有α2,3-和α2,8-活性的双官能唾液酸转移酶。 本发明还提供β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶。 糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。 在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

    Transformation system based on the integrase gene and attachment site for Myxococcus xanthus bacteriophage Mx9
    4.
    发明申请
    Transformation system based on the integrase gene and attachment site for Myxococcus xanthus bacteriophage Mx9 审中-公开
    基于整合酶基因和黄连球菌噬菌体Mx9的附着位点的转化体系

    公开(公告)号：US20040247620A1

    公开(公告)日：2004-12-09

    申请号：US10645818

    申请日：2003-08-20

    Applicant: Kosan Biosciences, Inc.

    Inventor： Bryan Julien

    IPC: A61K039/02 ,  C12N015/74 ,  C12N001/21

    CPC classification number: C12N15/74 ,  C12N15/102 ,  C12N15/90

    Abstract: The invention provides a transformation system based on bacteriophage Mx9, a temperate phage that infects Myxococcus xanthus. Vectors containing an integrase-encoding gene and a phage attachment site (attP) integrate into a chromosomal attB site and can be used to alter or introduce genes into a variety of host cells.

    Abstract translation: 本发明提供了一种基于噬菌体Mx9的转化系统，Mx9是一种感染黄色索氏杆菌的温带噬菌体。 含有整合酶编码基因和噬菌体附着位点（attP）的载体整合到染色体attB位点，可用于将基因改变或引入多种宿主细胞。

    Protein Hydrolysates Enriched In Peptides Having A Carboxy Terminal Proline Residue
    5.
    发明申请
    Protein Hydrolysates Enriched In Peptides Having A Carboxy Terminal Proline Residue 有权
    具有羧基末端脯氨酸残基的肽中丰富的蛋白质水解产物

    公开(公告)号：US20040241664A1

    公开(公告)日：2004-12-02

    申请号：US10450185

    申请日：2003-11-10

    Inventor： Petrus Jacobus Theodorus Dekker ,  Luppo Edens ,  Robertus Antonius Mijndert Van Der Hoeven ,  Linda De Lange

    IPC: C12Q001/68 ,  C07H021/04 ,  C12N009/50 ,  C12N001/16 ,  C12N015/74

    CPC classification number: G01N33/573 ,  A23J3/16 ,  A23J3/343 ,  A23J3/344 ,  A23J3/346 ,  A23L2/02 ,  A23L2/84 ,  A23L33/18 ,  A23L33/40 ,  C12H1/003 ,  C12Q1/37 ,  G01N33/56961 ,  G01N2333/38

    Abstract: The present invention provides An isolated polypeptide which has proline specific endoprotease activity, selected from the group consisting of:(a) a polypeptide which has an amino acid sequence which has at least 40% amino acid sequence identity with amino acids 1 to 526 of SEQ ID NO:2 or a fragment thereof;(b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO:1 or a fragment thereof which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO:1.

    Abstract translation: 本发明提供了具有脯氨酸特异性内切蛋白酶活性的分离多肽，其选自：（a）多肽，其具有与SEQ ID NO：1至526的氨基酸至少具有40％氨基酸序列同一性的氨基酸序列 或其片段;（b）由在低严格条件下与（i）SEQ ID NO：1的核酸序列或其片段至少80％的杂交的多核苷酸编码的多肽， 或超过200个核苷酸的90％以上，优选超过100个核苷酸，更优选至少90％相同，或（ii）与SEQ ID NO：1的核酸序列互补的核酸序列。

    Sialyltransferases from C. jejuni
    8.
    发明申请
    Sialyltransferases from C. jejuni 有权
    空肠弯曲杆菌的唾液酸转移酶

    公开(公告)号：US20040229313A1

    公开(公告)日：2004-11-18

    申请号：US10821573

    申请日：2004-04-08

    Applicant: National Research Council of Canada

    Inventor： Michel Gilbert ,  Warren W. Wakarchuk

    IPC: A61K031/739 ,  C07H021/04 ,  C12N009/10 ,  C12N001/21 ,  C12N015/74

    CPC classification number: C12N9/1241 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1081 ,  C12P19/18

    Abstract: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an null2,3- and an null2,8-activity. A null1,4-GalNAc transferase and a null1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

    Abstract translation: 本发明提供了原核糖基转移酶，包括具有α2,3-和α2,8-活性的双官能唾液酸转移酶。 本发明还提供β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶。 糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。 在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

    Methods and compositions for high level expression of a heterologous protein with poor codon usage
    9.
    发明申请
    Methods and compositions for high level expression of a heterologous protein with poor codon usage 审中-公开
    用于高水平表达具有差的密码子使用的异源蛋白质的方法和组合物

    公开(公告)号：US20040214281A1

    公开(公告)日：2004-10-28

    申请号：US10777010

    申请日：2004-02-11

    Inventor： Carsten-Peter Carstens

    IPC: C12P021/02 ,  C12N001/21 ,  C12N015/74

    CPC classification number: C12N15/11 ,  C12N15/70

    Abstract: High level expression of heterologous proteins in host cells is frequently limited by the presence of rarely utilized codons, due to depletion of the internal tRNA pool and stalling of translation. This invention provides expression host cells generated by introduction of a vector for the expression of an array of tRNA genes that are rare in the host cells. The modified host cells are capable of efficiently supporting expression of selected recombinant genes which otherwise would be limited by the presence of rare codons.

    Abstract translation: 宿主细胞中异源蛋白质的高水平表达常常受到很少使用的密码子的存在的限制，这是由于内部tRNA库的消耗和翻译失速。 本发明提供通过引入用于表达宿主细胞中罕见的tRNA基因阵列的载体产生的表达宿主细胞。 修饰的宿主细胞能够有效地支持选择的重组基因的表达，否则将被稀有密码子的存在所限制。

    Method for identifying constitutively active mutants of mitogen activated protein kinase (mapk) and uses thereof
    10.
    发明申请
    Method for identifying constitutively active mutants of mitogen activated protein kinase (mapk) and uses thereof 审中-公开
    用于鉴定丝裂原活化蛋白激酶（mapk）的组成型活性突变体的方法及其用途

    公开(公告)号：US20040214186A1

    公开(公告)日：2004-10-28

    申请号：US10474239

    申请日：2004-05-10

    Inventor： David Engelberg ,  Michal Bell ,  Alexander Levitzki

    IPC: C12Q001/68 ,  C12N001/18 ,  C12N015/74

    CPC classification number: C12N9/1205 ,  C12N15/1034

    Abstract: The present invention relates to a method of screening for constitutively active mutants of a desired eukaryotic MAPK pathway member of a MAPK pathway member, comprising the steps of (a) providing a mutant yeast strain devoid of an upstream kinase; (b) providing a DNA library of different mutants of a mutagenized gene coding for the desired MAPK pathway member; (c) introducing said library into said yeast strain under conditions suitable for activation of the yeast MAPK pathway; (d) detecting an end-point indication for activation of the yeast pathway; and (e) optionally isolating said constitutively activated mutant from selected rescued clones. The invention further relates to isolated constitutively active mutants of the MAPK pathway member, and their various uses particularly, in a method of screening for substances which are inhibitors of a MAPK pathway, and in drug design.

    Abstract translation: 本发明涉及筛选MAPK途径成员的所需真核MAPK通路成员的组成型活性突变体的方法，其包括以下步骤：（a）提供缺乏上游激酶的突变型酵母菌株; （b）提供编码所需MAPK通路成员的诱变基因的不同突变体的DNA文库; （c）在适于活化酵母MAPK途径的条件下将所述文库导入所述酵母菌株; （d）检测用于激活酵母途径的终点指示; 和（e）任选地从所选的拯救的克隆中分离所述组成型激活的突变体。 本发明还涉及MAPK通路成员的分离的组成型活性突变体及其各种用途，特别是在筛选作为MAPK途径的抑制剂的物质的方法和药物设计中。

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