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    Method of producing 2, 3-butanediol using recombinant yeast
    2.
    发明授权
    Method of producing 2, 3-butanediol using recombinant yeast 有权
    使用重组酵母生产2,3-丁二醇的方法

    公开(公告)号：US09328358B2

    公开(公告)日：2016-05-03

    申请号：US14293711

    申请日：2014-06-02

    申请人： SNU R&DB Foundation

    发明人： Jin Ho Seo ,  Soo Jung Kim ,  Seung Oh Seo

    IPC分类号： C12P7/18

    CPC分类号： C12P7/18 ,  C12Y101/01004 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y202/01006 ,  C12Y401/01005 ,  Y02P20/52

    摘要： Disclosed is a method of producing 2,3-butanediol using a recombinant yeast having a 2,3-butanediol biosynthesis pathway controlled in a metabolic engineering manner. The method enables production of 2,3-butanediol at high production efficiency from glucose or xylose using a recombinant yeast wherein enzymatic activity of pyruvate decarboxylase is inhibited and exotic genes associated with 2,3-butanediol biosynthesis are introduced.

    摘要翻译： 公开了以代谢工程方式控制的具有2,3-丁二醇生物合成途径的重组酵母生产2,3-丁二醇的方法。 该方法可以使用其中引入丙酮酸脱羧酶的酶活性并引入与2,3-丁二醇生物合成相关的外来基因的重组酵母从葡萄糖或木糖生产高生产效率的2,3-丁二醇。

    Strains of Saccharomyces cerevisiae
    5.
    发明授权
    Strains of Saccharomyces cerevisiae 有权
    酿酒酵母菌株

    公开(公告)号：US08962289B2

    公开(公告)日：2015-02-24

    申请号：US13885037

    申请日：2011-11-15

    申请人： Eva Albers ,  Lisbeth Olsson ,  Rakesh Koppram

    发明人： Eva Albers ,  Lisbeth Olsson ,  Rakesh Koppram

    IPC分类号： C12N1/12 ,  C12P7/06 ,  C12P7/10 ,  C12R1/865 ,  C12N9/04 ,  C12N15/01 ,  C12N15/81 ,  C12Q1/04 ,  C12N9/12

    CPC分类号： C12P7/06 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/01 ,  C12N15/81 ,  C12P7/10 ,  C12Q1/04 ,  C12R1/865 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

    摘要翻译： 描述了引入了编码木糖还原酶，木糖醇脱氢酶和木酮糖激酶的基因并且改进的乙醇生产，改善的木糖转化，降低的木糖醇产生和改进的抑制剂耐受性的酿酒酵母菌株的方法。 该方法包括在25-38℃，优选30-35℃的温度和0.040-0.055vvm的气流下以基本上仅含有木糖作为碳源的培养基以连续模式培养酿酒酵母菌株 ，并且增加稀释率以维持恒定的细胞水平，所述细胞水平在通过光密度或等效分析方法测定的1.5-3.0的范围内，并且向细胞中加入至少一种抑制剂并逐渐增加所述抑制剂的添加 。 此外，描述了通过根据本发明的方法获得的酿酒酵母菌株。

    Engineering fungi for the utilisation of l-arabinose
    6.
    发明申请
    Engineering fungi for the utilisation of l-arabinose 失效
    工程真菌用于利用阿拉伯糖

    公开(公告)号：US20030186402A1

    公开(公告)日：2003-10-02

    申请号：US10257821

    申请日：2003-03-10

    发明人： John Londesborough ,  Merja Penttilnull ,  Peter Richard

    IPC分类号： C12P007/06 ,  C12N001/18 ,  C12N001/16 ,  C12N015/74

    CPC分类号： C12N9/0006 ,  C12Y101/0101 ,  C12Y101/01012 ,  Y02E50/17

    摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

    摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。

    COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS
    8.
    发明申请
    COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS 审中-公开
    高效率异常路径的组合设计

    公开(公告)号：US20130295631A1

    公开(公告)日：2013-11-07

    申请号：US13877144

    申请日：2011-10-03

    申请人： Huimin Zhao ,  Byoungjin Kim ,  Jing Du ,  Yongbo Yuan ,  Dawn Eriksen ,  Tong Si

    发明人： Huimin Zhao ,  Byoungjin Kim ,  Jing Du ,  Yongbo Yuan ,  Dawn Eriksen ,  Tong Si

    IPC分类号： C12P7/06

    CPC分类号： C12P7/06 ,  C07K14/39 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/2445 ,  C12N15/1093 ,  C12N15/52 ,  C12N15/81 ,  C12P7/10 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  C12Y302/01021 ,  Y02E50/16 ,  Y02E50/17

    摘要： The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars.

    摘要翻译： 本公开涉及通过鉴定有利的酶和/或启动子组合在宿主细胞中产生高效的异源途径。 特别地，本公开提供了从真菌酶文库中组装和选择多步木糖和阿拉伯糖/木糖利用途径的方法。 本公开进一步提供含有有利的酶组合的组合物，以及表达这种组合的重组酵母以及用于戊糖的生物转化的方法。 还提供了包括通过利用不同强度的启动子的组合鉴定的有利的表达模式的组合物和方法。 本文提供了用于从不同强度的启动子文库中组装和选择多步木糖，阿拉伯糖/木糖和纤维二糖利用途径的方法。 本公开进一步提供了含有有利启动子控制的异源酶编码多核苷酸的组合物，以及表达这些酶的重组酵母，以及它们用于戊糖和/或己糖的生物转化的方法。

    SACCHAROMYCES CEREVISIAE STRAINS
    9.
    发明申请
    SACCHAROMYCES CEREVISIAE STRAINS 有权
    SACCHAROMYCES CEREISIAE STRAINS

    公开(公告)号：US20130295620A1

    公开(公告)日：2013-11-07

    申请号：US13885031

    申请日：2011-11-15

    申请人： Eva Albers ,  Lisbeth Olsson ,  Rakesh Koppram

    发明人： Eva Albers ,  Lisbeth Olsson ,  Rakesh Koppram

    IPC分类号： C12P7/06

    CPC分类号： C12P7/06 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/01 ,  C12N15/81 ,  C12P7/10 ,  C12Q1/04 ,  C12R1/865 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28-32° C., and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32° C. to obtain an increased selection pressure for improved xylose fermentation,improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

    摘要翻译： 描述了引入了编码木糖还原酶，木糖醇脱氢酶和木酮糖激酶的基因并且改进的乙醇生产，木糖转化和木糖醇生产降低的酿酒酵母菌株的制备方法。 该方法包括以约15-25g / l的木糖浓度和约28-32℃的温度在培养基中以重复批次序列培养细胞，然后在至少一个步骤中降低木糖浓度 在约28-32℃的温度下，以获得用于改进的木糖发酵，改进的乙醇生产和减少木糖醇生产的增加的选择压力，并且继续在所述重复批次系列中培养细胞。 此外，描述了通过根据本发明的方法获得的酿酒酵母菌株。

    STRAINS OF SACCHAROMYCES CEREVISIAE
    10.
    发明申请
    STRAINS OF SACCHAROMYCES CEREVISIAE 有权
    细菌的菌株

    公开(公告)号：US20130244247A1

    公开(公告)日：2013-09-19

    申请号：US13885037

    申请日：2011-11-15

    申请人： Eva Albers ,  Lisbeth Olsson ,  Rakesh Koppram

    发明人： Eva Albers ,  Lisbeth Olsson ,  Rakesh Koppram

    IPC分类号： C12N15/81 ,  C12Q1/04

    CPC分类号： C12P7/06 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/01 ,  C12N15/81 ,  C12P7/10 ,  C12Q1/04 ,  C12R1/865 ,  C12Y101/01009 ,  C12Y101/0101 ,  C12Y101/01307 ,  C12Y207/01017 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

    摘要翻译： 描述了引入了编码木糖还原酶，木糖醇脱氢酶和木酮糖激酶的基因并且改进的乙醇生产，改善的木糖转化，降低的木糖醇产生和改进的抑制剂耐受性的酿酒酵母菌株的方法。 该方法包括在25-38℃，优选30-35℃的温度和0.040-0.055vvm的气流下以基本上仅含有木糖作为碳源的培养基以连续模式培养酿酒酵母菌株 ，并且增加稀释率以维持恒定的细胞水平，所述细胞水平在通过光密度或等效分析方法测定的1.5-3.0的范围内，并且向细胞中加入至少一种抑制剂并逐渐增加所述抑制剂的添加 。 此外，描述了通过根据本发明的方法获得的酿酒酵母菌株。