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    Engineering fungi for the utilisation of L-arabinose
    2.
    发明授权
    Engineering fungi for the utilisation of L-arabinose 失效
    工程真菌利用L-阿拉伯糖

    公开(公告)号：US07527951B2

    公开(公告)日：2009-05-05

    申请号：US10257821

    申请日：2002-02-15

    申请人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    发明人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    IPC分类号： C12P7/06 ,  C12N1/18 ,  C12N1/16 ,  C12N15/74

    CPC分类号： C12N9/0006 ,  C12Y101/0101 ,  C12Y101/01012 ,  Y02E50/17

    摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

    摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。

    Engineering Fungi for the Utilisation of L-Arabinose
    3.
    发明申请
    Engineering Fungi for the Utilisation of L-Arabinose 审中-公开
    工程真菌利用L-阿拉伯糖

    公开(公告)号：US20090209016A1

    公开(公告)日：2009-08-20

    申请号：US12368509

    申请日：2009-02-10

    申请人： John Londesborough ,  Merja Penttila ,  Peter Richard

    发明人： John Londesborough ,  Merja Penttila ,  Peter Richard

    IPC分类号： C12P7/56 ,  C12P7/06 ,  C12P7/18 ,  C12P1/02

    CPC分类号： C12N9/0006 ,  C12Y101/0101 ,  C12Y101/01012 ,  Y02E50/17

    摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

    摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。

    Fungal micro-organism having an increased ability to carry out biotechnological process(es)
    5.
    发明申请
    Fungal micro-organism having an increased ability to carry out biotechnological process(es) 审中-公开
    具有提高生物技术过程能力的真菌微生物

    公开(公告)号：US20050106734A1

    公开(公告)日：2005-05-19

    申请号：US10494030

    申请日：2002-10-29

    申请人： Peter Richard ,  John Londesborough ,  Merja Penttila ,  Ritva Verho

    发明人： Peter Richard ,  John Londesborough ,  Merja Penttila ,  Ritva Verho

    IPC分类号： C12N15/09 ,  C12N1/14 ,  C12N1/19 ,  C12N9/02 ,  C12P7/06 ,  C12P7/62 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N9/0008 ,  Y02E50/17

    摘要： The present invention relates to fungal microorganism having an increased ability to carry out biotechnological process(es). In particular, the invention relates to improving the regeneration of redox cofactors in biotechnological processes where useful products are produced from biomass containing pentoses. According to the invention, the microorganism is transformed with a DNA sequence encoding an NADP linked glyceraldehyde 3-phosphate dehydrogenase. The invention can be used to provide useful products for mankind from biological materials, including e.g. agricultural and forestry products, municipal waste. Examples of such useful products are ethanol, lactic acid, polyhydroxyalkanoates, amino acids, fats, vitamins, nucleotides and a wide variety of enzymes and pharmaceuticals.

    摘要翻译： 本发明涉及具有提高生物技术过程能力的真菌微生物。 特别地，本发明涉及在生物技术过程中改进氧化还原辅因子的再生，其中有用产物由含有戊糖的生物质产生。 根据本发明，用编码NADP连接的甘油醛-3-磷酸脱氢酶的DNA序列转化微生物。 本发明可用于从生物材料为人类提供有用的产品，包括例如， 农林产品，城市垃圾。 这些有用产品的实例是乙醇，乳酸，聚羟基链烷酸酯，氨基酸，脂肪，维生素，核苷酸和多种酶和药物。

    Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
    9.
    发明授权
    Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase 失效
    通过用海藻糖合成酶的结构基因的组合转化生物体来增加生物体的海藻糖含量

    公开(公告)号：US06323001B1

    公开(公告)日：2001-11-27

    申请号：US09013598

    申请日：1998-01-26

    申请人： John Londesborough ,  Outi Tunnela ,  Tapio Palva ,  Kjell-Ove Holmström ,  Björn Welin ,  Abul Mandal

    发明人： John Londesborough ,  Outi Tunnela ,  Tapio Palva ,  Kjell-Ove Holmström ,  Björn Welin ,  Abul Mandal

    IPC分类号： C12N1500

    CPC分类号： C07K14/395 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/92 ,  C12N15/8245

    摘要： Two nucleotide sequences encoding two different polypeptides found in yeast trehalose synthase have been isolated and cloned. A third polypeptide has been isolated from the enzyme and characterized, and a method is provided to isolate and clone the nucleotide sequence encoding this polypeptide. The coding sequences can be inserted into suitable vectors and used to transform host cells. The transformed cells will produce increased amounts of trehalose compared to the untransformed wild types and have increased tolerance to a variety of stresses, in particular to decreased availability of water. The invention may be used to improve the stress tolerance of organisms, to increase the storage life of foodstuffs and to produce trehalose economically on an industrial scale in an organism (e.g. baker's yeast) that is a traditional and safe foodstuff.

    摘要翻译： 已经分离并克隆了在酵母海藻糖合成酶中发现的编码两种不同多肽的两个核苷酸序列。 从酶中分离出第三种多肽，并进行表征，并提供了分离和克隆编码该多肽的核苷酸序列的方法。 编码序列可以插入合适的载体并用于转化宿主细胞。 与未转化的野生型相比，转化的细胞将产生增加量的海藻糖，并且具有增加的对各种胁迫的耐受性，特别是降低水的可利用性。 本发明可用于改善生物体的胁迫耐受性，增加食品的储存寿命，并在工业规模上经济地生产海藻糖，其为传统和安全食品的生物（例如面包酵母）。

    Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase
    10.
    发明授权
    Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase 失效
    通过用酵母海藻糖合成酶的短链和长链的结构基因转化生物体来增加生物体的海藻糖含量的方法

    公开(公告)号：US5422254A

    公开(公告)日：1995-06-06

    申请号：US841997

    申请日：1992-02-28

    申请人： John Londesborough ,  Outi Vuorio

    发明人： John Londesborough ,  Outi Vuorio

    IPC分类号： C12N15/09 ,  C07K14/395 ,  C12N9/10 ,  C12N9/14 ,  C12N9/16 ,  C12N9/92 ,  C12N15/82 ,  C12P19/12 ,  C12R1/865 ,  C12P19/18

    CPC分类号： C12N9/16 ,  C07K14/395 ,  C12N15/8245 ,  C12N9/1051 ,  C12N9/92 ,  Y10S435/942

    摘要： Two nucleotide sequences encoding two different polypeptides found in yeast trehalose synthase have been isolated and cloned. The coding sequences can be inserted into suitable vectors and used to transform host cells. The transformed cells will produce increased amounts of trehalose compared to the untransformed wild types and have increased tolerance to a variety of stresses, in particular to decreased availability of water. The invention may be used to improve the stress tolerance of organisms, to increase the storage life of foodstuffs and to produce trehalose economically on an industrial scale in an organism (e.g, baker's yeast) that is a traditional and safe foodstuff.

    摘要翻译： 已经分离并克隆了在酵母海藻糖合成酶中发现的编码两种不同多肽的两个核苷酸序列。 编码序列可以插入合适的载体并用于转化宿主细胞。 与未转化的野生型相比，转化的细胞将产生增加量的海藻糖，并且具有增加的对各种胁迫的耐受性，特别是降低水的可用性。 本发明可用于改善生物体的胁迫耐受性，增加食品的储存寿命并且在工业规模上经济地生产作为传统和安全食品的生物体（例如面包酵母）中的海藻糖。