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    METHOD FOR THE DETERMINATION OF BOTULINUM NEUROTOXIN BIOLOGICAL ACTIVITY
    2.
    发明申请
    METHOD FOR THE DETERMINATION OF BOTULINUM NEUROTOXIN BIOLOGICAL ACTIVITY 有权
    测定嗜碱性粒细胞生物活性的方法

    公开(公告)号：US20150284702A1

    公开(公告)日：2015-10-08

    申请号：US14747139

    申请日：2015-06-23

    申请人： MERZ PHARMA GmbH & CO. KGaA

    发明人： Klaus FINK ,  Martin Vey

    IPC分类号： C12N9/64

    CPC分类号： C12N9/6472 ,  C12N9/6475 ,  C12Q1/37 ,  C12Y304/22055 ,  C12Y304/22056 ,  C12Y304/22059 ,  C12Y304/2206 ,  C12Y304/22061 ,  C12Y304/22062 ,  C12Y304/22063 ,  G01N33/5014 ,  G01N2333/33 ,  G01N2510/00

    摘要： The present invention relates to means and methods for determining neurotoxin activity. Specifically, it relates to a polypeptide having caspase activity comprising a large subunit and a small subunit wherein the caspase further comprises a neurotoxin cleavage site which upon cleavage activates the caspase activity. Also encompassed are polynucleotides encoding the polypeptides as well as vectors or host cells comprising the polynucleotides. The present invention further relates to a method for determining neurotoxin activity in a sample based on the polypeptide of the invention as well as the use of the polypeptide for determining neurotoxin activity in a sample, in general.

    摘要翻译： 本发明涉及测定神经毒素活性的方法和方法。 具体地说，本发明涉及一种具有半胱天冬酶活性的多肽，其包含大亚基和小亚基，其中半胱天冬酶还包含神经毒素切割位点，其在切割时激活半胱天冬酶活性。 还包括编码多肽的多核苷酸以及包含多核苷酸的载体或宿主细胞。 本发明还涉及基于本发明的多肽测定样品中的神经毒素活性的方法，以及通常用于测定样品中的神经毒素活性的多肽的用途。

    METHOD FOR THE DETERMINATION OF BOTULINUM NEUROTOXIN BIOLOGICAL ACTIVITY
    3.
    发明申请
    METHOD FOR THE DETERMINATION OF BOTULINUM NEUROTOXIN BIOLOGICAL ACTIVITY 有权
    测定嗜碱性粒细胞生物活性的方法

    公开(公告)号：US20140004541A1

    公开(公告)日：2014-01-02

    申请号：US14004259

    申请日：2012-03-09

    申请人： Klaus Fink ,  Martin Vey

    发明人： Klaus Fink ,  Martin Vey

    IPC分类号： C12Q1/37 ,  C12N9/64

    CPC分类号： C12N9/6472 ,  C12N9/6475 ,  C12Q1/37 ,  C12Y304/22055 ,  C12Y304/22056 ,  C12Y304/22059 ,  C12Y304/2206 ,  C12Y304/22061 ,  C12Y304/22062 ,  C12Y304/22063 ,  G01N33/5014 ,  G01N2333/33 ,  G01N2510/00

    摘要： The present invention relates to means and methods for determining neurotoxin activity. Specifically, it relates to a polypeptide having caspase activity comprising a large subunit and a small subunit wherein the caspase further comprises a neurotoxin cleavage site which upon cleavage activates the caspase activity. Also encompassed are polynucleotides encoding the polypeptides as well as vectors or host cells comprising the polynucleotides. The present invention further relates to a method for determining neurotoxin activity in a sample based on the polypeptide of the invention as well as the use of the polypeptide for determining neurotoxin activity in a sample, in general.

    摘要翻译： 本发明涉及测定神经毒素活性的方法和方法。 具体地说，本发明涉及一种具有半胱天冬酶活性的多肽，其包含大亚基和小亚基，其中半胱天冬酶还包含神经毒素切割位点，其在切割时激活半胱天冬酶活性。 还包括编码多肽的多核苷酸以及包含多核苷酸的载体或宿主细胞。 本发明还涉及基于本发明的多肽测定样品中的神经毒素活性的方法，以及通常用于测定样品中的神经毒素活性的多肽的用途。

    Molecular vaccines employing nucleic acid encoding anti-apoptotic proteins
    4.
    发明申请
    Molecular vaccines employing nucleic acid encoding anti-apoptotic proteins 审中-公开
    使用编码抗凋亡蛋白的核酸的分子疫苗

    公开(公告)号：US20070026076A1

    公开(公告)日：2007-02-01

    申请号：US10546810

    申请日：2004-02-24

    申请人： Tzyy-Choou Wu ,  Chien Hung ,  Tae-Woo Kim

    发明人： Tzyy-Choou Wu ,  Chien Hung ,  Tae-Woo Kim

    IPC分类号： A61K48/00 ,  C12N15/09 ,  C12N15/87 ,  A61K9/14

    CPC分类号： A61K39/12 ,  A61K9/0019 ,  A61K38/4873 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55516 ,  A61K2039/585 ,  A61K2039/6006 ,  A61K2039/6031 ,  A61K2039/6043 ,  C07K14/005 ,  C07K14/4747 ,  C07K2319/01 ,  C07K2319/02 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20034 ,  C12N2800/107 ,  C12Y304/22036 ,  C12Y304/22055 ,  C12Y304/22056 ,  C12Y304/22057 ,  C12Y304/22058 ,  C12Y304/22059 ,  C12Y304/2206 ,  C12Y304/22061 ,  C12Y304/22062 ,  C12Y304/22063 ,  C12Y304/22064

    摘要： T cell immune responses are enhanced by presentation of antigen to CD8+ T cells using a chimeric nucleic acid immunogen or vaccine that links DNA encoding an antigen with DNA encoding a polypeptide that targets or translocates the antigenic polypeptide to which it is fused (immunogenicity-potentiating polypeptides or “IPP”). By inhibiting apoptosis in the vicinity of a T cell responses to such a nucleic acid immunogen, even more potent immune responses are attained. The present strategy prolongs the survival of DNA-transduced cells, including dendritic cells (DCs), thereby enhancing the priming of antigen-specific T cells and increase potency. Co-delivery of DNA encoding an inhibitor of apoptosis, including (a) BCL-xL, (b) BCL-2, (c) XIAP, (d) dominant negative caspase-9, or (e) dominant negative caspase-8, or (f) serine protease inhibitor 6 (SPI-6) which inhibits granzyme B, with DNA encoding an antigen, prolongs the survival of transduced DCs and results in significant enhancement of antigenspecific T cell immune responses that provide potent antitumor effects. Thus, co-administration of a DNA vaccine encoding antigen linked to an IPP along with one or more DNA constructs encoding an anti-apoptotic protein provides a novel way to enhance vaccine potency.

    摘要翻译： 使用将编码抗原的编码抗原的DNA与编码靶向或转位抗原多肽的多肽连接的嵌合核酸免疫原或疫苗将CD8 + T细胞呈递抗原来增强T细胞免疫应答， 它是融合的（免疫原性增强多肽或“IPP”）。 通过抑制对这种核酸免疫原的T细胞应答附近的细胞凋亡，获得更强大的免疫应答。 目前的策略延长了DNA转导细胞（包括树突状细胞（DCs））的存活，从而增强了抗原特异性T细胞的引发并提高了效力。 包括（a）BCL-xL，（b）BCL-2，（c）XIAP，（d）显性负性胱天蛋白酶-9或（e）显性负性胱天蛋白酶-8的DNA的编码凋亡抑制剂的DNA共转染， 或（f）用编码抗原的DNA抑制粒酶B的丝氨酸蛋白酶抑制剂6（SPI-6）延长了转导的DC的存活并且导致提供有效的抗肿瘤作用的抗原特异性T细胞免疫应答的显着增强。 因此，编码与IPP连接的抗原的DNA疫苗与编码抗凋亡蛋白的一种或多种DNA构建体的共同给药提供了增强疫苗效力的新方法。

    Antisense compounds which prevent cell death and uses thereof
    9.
    发明申请
    Antisense compounds which prevent cell death and uses thereof 有权
    防止细胞死亡的反义化合物及其用途

    公开(公告)号：US20030092659A1

    公开(公告)日：2003-05-15

    申请号：US10185084

    申请日：2002-06-28

    发明人： Carol M. Troy ,  Michael L. Shelanski

    IPC分类号： A61K048/00 ,  A61K038/10 ,  C07H021/04 ,  C07K007/08

    CPC分类号： C12N15/1137 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/3513 ,  C12Y304/22055

    摘要： The present invention provides for an antisense oligonucleotide having the sequence 5nullGCTCGGCGCCGCCATTTCCAG3null. The invention also provides for an antisense oligonucleotide having the sequence 5nullGTCAGCGGCCATCAGCTT3null. The present invention further provides for a method for treating a neurodegenerative disorder in a subject which comprises administering to the subject a compound in an amount effective to inhibit neuronal cell death and thus treat the neurodegenerative disorder in the subject, which compound comprises the oligonucleotide 5nullGCTCGGCGCCGCCATTTCCAG3null and a delivery agent. The present invention provides for a method of inhibiting trophic factor withdrawal mediated death of a cell which comprises contacting the cell with an amount of the oligonucleotide 5nullGCTCGGCGCCGCCATTTCCAG3null effective to inhibit death of the cell.

    摘要翻译： 本发明提供具有5'GCTCGGCGCCGCCATTTCCAG3'序列的反义寡核苷酸。 本发明还提供具有5'GTCAGCGGCCATCAGCTT3'序列的反义寡核苷酸。 本发明进一步提供一种治疗受试者神经变性疾病的方法，其包括以有效抑制神经元细胞死亡的量向受试者施用化合物，从而治疗受试者的神经变性疾病，该化合物包含寡核苷酸5' GCTCGGCGCCGCCATTTCCAG3'和递送剂。 本发明提供了抑制营养因子撤除介导的细胞死亡的方法，其包括使细胞与一定量的有效抑制细胞死亡的寡核苷酸5'GCTCGGCGCCGCCATTTCCAG3'接触。