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12 条记录 (耗时 0.016 秒)

    Purification of a pertussis outer membrane protein
    2.
    发明授权
    Purification of a pertussis outer membrane protein 有权

    公开(公告)号：US06444211B1

    公开(公告)日：2002-09-03

    申请号：US09327527

    申请日：1999-06-08

    申请人： Gail Jackson ,  Raafat Fahim ,  Larry Tan ,  Pele Chong ,  John Vose ,  Michel Klein

    发明人： Gail Jackson ,  Raafat Fahim ,  Larry Tan ,  Pele Chong ,  John Vose ,  Michel Klein

    IPC分类号： A61K3910

    摘要： Pertactin (formerly 69 kDa protein) is recovered in stable biologically pure form having no detectable adenylate cyclase activity from fermentation broth from the fermentation of Bordetella pertussis as well as from the cells. The broth is processed to selectively remove pertussis toxin (PT) and filamentous haemagglutinin (FHA), the pertactin is precipitated by ammonium sulphate and the precipitate is dissolved in buffer at pH 6.0 to 8.5, the solution then is passed through hydroxyapatite and ion-exchange chromatograph columns before final ultrafiltration. Cells are extracted with urea and the extract ultrafiltered and diafiltered. The pertactin is precipitated from the extract and the precipitate processed as above. In a variation, the broth is contacted with ammonium sulphate to precipitate pertactin, PT and FHA, the precipitate is dissolved and the PT and FHA selectively removed, before the solution is passed to the chromatograph columns.

    Methods for prevention and/or treatment of thrombocytopenia
    3.
    发明授权
    Methods for prevention and/or treatment of thrombocytopenia 失效
    预防和/或治疗血小板减少症的方法

    公开(公告)号：US06183756B2

    公开(公告)日：2001-02-06

    申请号：US08846461

    申请日：1997-05-01

    申请人： Nicolae Istrate ,  Gita Muni ,  Edgard Brauner ,  Fazal Raheman

    发明人： Nicolae Istrate ,  Gita Muni ,  Edgard Brauner ,  Fazal Raheman

    IPC分类号： A61K3910

    CPC分类号： A61K35/74

    摘要： The present invention is directed to a method of treating subjects exhibiting thrombocytopenia or at risk of developing thrombocytopenia. The method includes the step of administering to the subject exhibiting thrombocytopenia or at risk of developing thrombocytopenia, an effective amount of a Pseudomonas solvent extract or active fraction thereof. The Pseudomonas solvent extract is administered in an amount effective to increase platelet levels in the subject.

    摘要翻译： 本发明涉及治疗表现出血小板减少症或有发生血小板减少症风险的受试者的方法。 该方法包括给予呈现血小板减少症或存在发生血小板减少症风险的受试者有效量的假单胞菌属溶剂提取物或其活性组分的步骤。 以有效提高受试者血小板水平的量施用假单胞菌溶剂提取物。

    Non-pyrogenic bacterial strains and use of the same
    4.
    发明授权
    Non-pyrogenic bacterial strains and use of the same 失效
    非热原菌菌株和使用相同

    公开(公告)号：US06548287B1

    公开(公告)日：2003-04-15

    申请号：US09339180

    申请日：1999-06-24

    申请人： Robert J. Powell ,  David M. Hone

    发明人： Robert J. Powell ,  David M. Hone

    IPC分类号： A61K3910

    CPC分类号： C12R1/01 ,  A61K48/00 ,  C12N15/74 ,  C12P19/04

    摘要： The present invention provides gram-negative bacterial strains that produce substantially pure non-pyrogenic lipopolysaccharide or lipid A. The present invention also relates to a use of said strains for the preparation of non-pyrogenic DNA and use of the same for introducing endogenous or foreign genes into animal cells or animal tissue. Further, the present invention relates to a use of said strains for the preparation of non-pyrogenic recombinant mammalian, protozoan and viral proteins. Furthermore, the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial vaccines and vaccine vectors. Yet a further use of the present invention relates to a use of said strains for the preparation of non-pyrogenic bacterial proteins and polysaccharides antigens for use as vaccines.

    摘要翻译： 本发明提供了产生基本上纯的非致热性脂多糖或脂质A的革兰氏阴性细菌菌株。本发明还涉及所述菌株用于制备非致热原DNA的用途，并且使用该菌株用于引入内源或外源 基因进入动物细胞或动物组织。 此外，本发明涉及所述菌株用于制备非致热性重组哺乳动物，原生动物和病毒蛋白质的用途。 此外，本发明涉及所述菌株用于制备非致热性细菌疫苗和疫苗载体的用途。 本发明的进一步应用涉及所述菌株用于制备用作疫苗的非致热性细菌蛋白质和多糖抗原的用途。

    Acellular antibordetella vaccine
    5.
    发明授权
    Acellular antibordetella vaccine 失效
    无细胞抗菌肽疫苗

    公开(公告)号：US06387377B1

    公开(公告)日：2002-05-14

    申请号：US08574439

    申请日：1995-12-15

    申请人： Pascale Gueirard ,  Nicole Guiso

    发明人： Pascale Gueirard ,  Nicole Guiso

    IPC分类号： A61K3910

    CPC分类号： C12N9/88 ,  A61K39/00 ,  A61K2039/52 ,  C07K14/235 ,  C12R1/01 ,  Y10S435/814

    摘要： The invention relates to an immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (AC-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from B. Tertussis, B. parapertussis or B. bronchiseptica, and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.

    摘要翻译： 本发明涉及一种免疫原性组合物，其特征在于其包含选自B族百日咳杆菌，百日咳杆菌或百日咳杆菌的博德特氏菌菌株的腺苷酸环化酶 - 溶血素（AC-Hly）蛋白或该AC-Hly的免疫原性部分 支气管炎支气管炎，并且其还包含含有选自百日咳百日咳杆菌，副百日咳杆菌或支气管炎支气管炎的博德特氏菌的vrg基因的表达产物的细菌提取物，或这些表达产物的一部分 其足以在可以施用该提取物的宿主中诱导免疫应答。

    Vaccine compositions for mucosal delivery
    7.
    发明授权
    Vaccine compositions for mucosal delivery 失效
    用于粘膜递送的疫苗组合物

    公开(公告)号：US06562352B1

    公开(公告)日：2003-05-13

    申请号：US08963129

    申请日：1997-10-28

    申请人： Mark Roberts ,  Gordon Dougan

    发明人： Mark Roberts ,  Gordon Dougan

    IPC分类号： A61K3910

    CPC分类号： A61K39/08 ,  A61K39/099 ,  A61K2039/541 ,  A61K2039/70

    摘要： The invention provides the use of an antigen which is a mucosally immunogenically active substance comprising the 50 kD C fragment of tetanus toxin, an immunogenic fragment thereof, or a derivative thereof formed by amino acid deletion, substitution or insertion for the manufacture of a vaccine composition for administration to a mucosal surface to induce an immune response in the mucosal surface against tetanus infection. The Vaccine composition preferably contains the P.69 outer membrane protein of B. pertussis, and B. pertussis filamentous haemaglutiuin. The invention also provides vaccine compositions per se and a method of treating tetanus and optionally whooping cough using the vaccine compositions.

    摘要翻译： 本发明提供了抗原，其是包含破伤风毒素的50kD C片段的免疫原性活性物质，其免疫原性片段或其通过氨基酸缺失，取代或插入形成的衍生物，用于制备疫苗组合物 用于施用于粘膜表面以诱导粘膜表面中针对破伤风感染的免疫应答。 疫苗组合物优选含有百日咳杆菌的P.69外膜蛋白质和百日咳杆菌丝状血凝素。 本发明还提供疫苗组合物本身以及使用疫苗组合物治疗破伤风和任选的百日咳的方法。