公开(公告)号：US06682915B2

公开(公告)日：2004-01-27

申请号：US10052388

申请日：2002-01-23

申请人： Thomas Hermann ,  Achim Marx ,  Walter Pfefferle ,  Mechthild Rieping

发明人： Thomas Hermann ,  Achim Marx ,  Walter Pfefferle ,  Mechthild Rieping

IPC分类号： C12P746

CPC分类号： F04B53/143 ,  A23K20/174 ,  B60T8/4031 ,  C12N15/52 ,  C12P13/02 ,  F04B1/0408

摘要： A method for the fermentative preparation of D-pantothenic acid and/or its salts or feedstuff additives containing these. Said method comprising, fermentation of Escherichia coli microorganisms wherein the nucleotide sequence(s) coding the glyA gene is overexpressed.

摘要翻译： 用于发酵制备含有它们的D-泛酸和/或其盐或饲料添加剂的方法。 所述方法包括大肠杆菌微生物的发酵，其中编码glyA基因的核苷酸序列被过表达。

公开(公告)号：US06265190B1

公开(公告)日：2001-07-24

申请号：US09389641

申请日：1999-09-02

申请人： Sanjay Yedur ,  Kris A. Berglund ,  Dilum D. Dunuwila

发明人： Sanjay Yedur ,  Kris A. Berglund ,  Dilum D. Dunuwila

IPC分类号： C12P746

CPC分类号： C12P7/46 ,  Y10S435/822

摘要： A highly efficient process for the production and recovery of pure succinic acid from a succinate salt that involves minimal use of additional reagents, and produces virtually no waste by-products, and permits internal recycle of the base and acid values, is provided. The method involves the formation of diammonium succinate, either by using an ammonium ion based material to maintain neutral8 pH in the fermenter or by substituting the ammonium cation for the cation of the succinate salt created in the fermenter. The diammonium succinate can then be reacted with a sulfate ion, such as by combining the diammonium succinate with ammonium bisulfate and/or sulfuric acid at sufficiently low pH to yield succinic acid and ammonium sulfate. The ammonium sulfate is advantageously cracked thermally into ammonia and ammonium bisulfate. The succinic acid can be purified with a methanol dissolution step. Various filtration, reflux and reutilization steps can also be employed.

摘要翻译： 提供了从琥珀酸盐生产和回收纯琥珀酸的高效方法，其涉及最少使用另外的试剂，并且几乎不产生废物副产物，并且允许碱的内部循环和酸值。 该方法包括通过使用铵离子基材料在发酵罐中保持中性8 pH或通过用铵阳离子代替在发酵罐中产生的琥珀酸盐的阳离子来形成琥珀酸二铵。 然后可以将琥珀酸二铵与硫酸根离子反应，例如通过在足够低的pH下将琥珀酸二铵与硫酸氢铵和/或硫酸组合以产生琥珀酸和硫酸铵。 硫酸铵有利地热裂解成氨和硫酸氢铵。 琥珀酸可以用甲醇溶解步骤纯化。 也可以使用各种过滤，回流和再利用步骤。

公开(公告)号：US06689591B2

公开(公告)日：2004-02-10

申请号：US10041968

申请日：2002-01-07

申请人： Michael Müller ,  Michael Wolberg ,  Werner Hummel ,  Christian Wandrey

发明人： Michael Müller ,  Michael Wolberg ,  Werner Hummel ,  Christian Wandrey

IPC分类号： C12P746

CPC分类号： C12P17/04 ,  C12P7/62 ,  C12P41/002

摘要： In a method of reducing diketocarboxylic acids or hydroxyketocarboxylic acids or their esters, at least one keto group is converted to a hydroxyl group in the presence of lactobacillus species.

摘要翻译： 在还原二酮羧酸或羟基酮羧酸或其酯的方法中，在乳杆菌物种存在下，至少一个酮基转化为羟基。

公开(公告)号：US06686183B2

公开(公告)日：2004-02-03

申请号：US10052269

申请日：2002-01-23

申请人： Mechthild Rieping ,  Thomas Hermann

发明人： Mechthild Rieping ,  Thomas Hermann

IPC分类号： C12P746

CPC分类号： C12N15/52 ,  A23K20/105 ,  A23K20/158 ,  A23K20/174 ,  C12P13/02

摘要： A method br the fermentative preparation of D-pantothenic acid and/or its salts or feedstuff additives containing these. Said method comprising fermentation of Escherichia coli microorganisms wherein at least one of the nucleotide sequence(s) coding for the gcvT, gcvH, and gcvP gene is overexpressed.

摘要翻译： D-泛酸的发酵制剂和/或含有它们的盐或饲料添加剂的方法。 所述方法包括大肠杆菌微生物的发酵，其中编码gcvT，gcvH和gcvP基因的核苷酸序列中的至少一个被过表达。

公开(公告)号：US06777213B2

公开(公告)日：2004-08-17

申请号：US10640239

申请日：2003-08-13

申请人： Michael D. Staley

发明人： Michael D. Staley

IPC分类号： C12P746

CPC分类号： C12P7/6427 ,  C07C51/36 ,  C07C51/48 ,  C12P7/6409 ,  C07C55/02

摘要： A process for recovering carboxylic acids from an aqueous mixture such as a fermentation broth using a solvent containing at least one olefin without the need for first removing the spent microorganism cells is provided. A co-solvent which increases the partition coefficient of the solvent relative to the carboxylic acid may optionally be included. The resulting carboxylic acid is hydrogenated to produce a saturated carboxylic acid.

摘要翻译： 提供了一种使用含有至少一种烯烃的溶剂从水性混合物如发酵液中回收羧酸的方法，而不需要首先除去废的微生物细胞。 可以任选地包括增加溶剂相对于羧酸的分配系数的共溶剂。 将所得羧酸氢化以产生饱和羧酸。

公开(公告)号：US06743610B2

公开(公告)日：2004-06-01

申请号：US09823949

申请日：2001-03-30

申请人： Mark I. Donnelly ,  Cynthia Y. Sanville-Millard ,  Nhuan Phu Nghiem

发明人： Mark I. Donnelly ,  Cynthia Y. Sanville-Millard ,  Nhuan Phu Nghiem

IPC分类号： C12P746

CPC分类号： C12P7/46 ,  C12R1/19

摘要： A method for producing succinic acid from industrial-grade hydrolysates is provided, comprising supplying an organism that contains mutations for the genes ptsG, pflB, and ldhA, allowing said organism to accumulate biomass, and allowing said organism to metabolize the hydrolysate. Also provided is a bacteria mutant characterized in that it produces succinic acid from substrate contained in industrial-grade hydrolysate in a ratio of between 0.6:1 and 1.3:1 succinic acid to substrate.

摘要翻译： 提供了一种从工业级水解产物生产琥珀酸的方法，包括提供含有ptsG，pflB和ldhA基因突变的生物体，允许所述生物体积累生物质，并允许所述生物体代谢水解产物。 还提供了一种细菌突变体，其特征在于其以0.6:1至1.3:1琥珀酸与底物的比例从工业级水解产物中所含的底物产生琥珀酸。

公开(公告)号：US06331420B1

公开(公告)日：2001-12-18

申请号：US09302620

申请日：1999-04-30

申请人： C. Ron Wilson ,  David L. Craft ,  L. Dudley Eirich ,  Mark Eshoo ,  Krishna M. Madduri ,  Cathy A. Cornett ,  Alfred A. Brenner ,  Maria Tang ,  John C. Loper ,  Martin Gleeson

发明人： C. Ron Wilson ,  David L. Craft ,  L. Dudley Eirich ,  Mark Eshoo ,  Krishna M. Madduri ,  Cathy A. Cornett ,  Alfred A. Brenner ,  Maria Tang ,  John C. Loper ,  Martin Gleeson

IPC分类号： C12P746

CPC分类号： C12N9/0042 ,  C07K14/40 ,  C12N9/0071 ,  C12N9/0077 ,  C12P7/44

摘要： Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &ohgr;-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

摘要翻译： 已经分离出编码热带假丝酵母20336的ω-羟化酶复合物的细胞色素P450和NADPH还原酶的新型基因。提供了包括这些基因，转染的宿主细胞和转化的宿主细胞的载体。 还提供了生产细胞色素P450和NADPH还原酶的方法，其涉及用编码这些酶的基因转化宿主细胞并培养细胞。 还提供了增加二羧酸生产的方法和增加上述酶的生产的方法，其涉及在宿主细胞中增加编码这些酶的基因的数量。 还提供了通过定量基因表达来区分基因家族成员的方法。