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    Process For Producing A Fermentation Product
    2.
    发明申请
    Process For Producing A Fermentation Product 有权

    公开(公告)号：US20220186266A1

    公开(公告)日：2022-06-16

    申请号：US17600767

    申请日：2020-04-02

    申请人： Novozymes A/S

    发明人： Nathaniel Edward KREEL

    IPC分类号： C12P7/14 ,  C12P19/14 ,  C12N9/26 ,  C12N9/34 ,  C12N9/42 ,  C12N9/44 ,  C12P19/04 ,  C12P19/02 ,  C12P19/12

    摘要： The present invention relates to processes of producing a fermentation product from starch containing material comprising (a) forming a slurry comprising the starch-containing material and water; (b) converting the starch-containing material into dextrins with an alpha-amylase; (c) saccharifying the dextrins using a carbohydrate source generating enzyme to form sugars; (d) fermenting sugars using a fermenting organism; (e) recovering the fermentation product to form whole stillage; (f) separating the whole stillage into a liquid fraction thin stillage and solid fraction wet cake; (g) hydrolyzing the thin stillage; (h) recycle a portion of the hydrolyzed thin stillage to steps (a); wherein the thin stillage in step (g) is hydrolyzed using a glucoamylase and/or polygalacturonase.

    Compositions for Producing Glucose Syrups
    3.
    发明申请
    Compositions for Producing Glucose Syrups 有权

    公开(公告)号：US20210395791A1

    公开(公告)日：2021-12-23

    申请号：US17465098

    申请日：2021-09-02

    申请人： Novozymes A/S

    发明人： Shiro Fukuyama ,  Keiichi Ayabe

    IPC分类号： C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

    摘要： The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

    Compositions for producing glucose syrups
    5.
    发明授权
    Compositions for producing glucose syrups 有权

    公开(公告)号：US11136607B2

    公开(公告)日：2021-10-05

    申请号：US16403919

    申请日：2019-05-06

    申请人： Novozymes A/S

    发明人： Shiro Fukuyama ,  Keiichi Ayabe

    IPC分类号： C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

    摘要： The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

    Pullulanase variants and polynucleotides encoding same
    6.
    发明授权
    Pullulanase variants and polynucleotides encoding same 有权

    公开(公告)号：US11136568B2

    公开(公告)日：2021-10-05

    申请号：US16837166

    申请日：2020-04-01

    申请人： Novozymes A/S

    发明人： Tomoko Matsui ,  Akihiko Yamagishi

    IPC分类号： C12N9/44 ,  C12P19/16 ,  C12P19/02 ,  C12P7/06

    摘要： The present invention relates to pullulanase variants comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.