公开(公告)号：US11999928B2

公开(公告)日：2024-06-04

申请号：US17684693

申请日：2022-03-02

申请人： Novozymes A/S

发明人： Anne Mette Bhatia Frederiksen ,  Lars Beier ,  Stefan Kreisz

IPC分类号： C12C5/00 ,  C12C7/047 ,  C12N9/28 ,  C12N9/44

CPC分类号： C12C5/004 ,  C12C7/047 ,  C12N9/2417 ,  C12N9/2457 ,  C12Y302/01041

摘要： A method of mashing comprising providing a grist comprising malt and adjunct; and contacting the grist with a pullulanase; an alpha amylase; and a maltogenic alpha amylase and/or a beta amylase to make a wort. An enzyme composition comprising a pullulanase; an alpha amylase; and a maltogenic alpha amylase and/or a beta amylase and the use of these enzymes in brewing is disclosed.

公开(公告)号：US20230242680A1

公开(公告)日：2023-08-03

申请号：US18299303

申请日：2023-04-12

申请人： CORN PRODUCTS DEVELOPMENT, INC.

发明人： Laercio ARNONI ,  Jose BERTOLI ,  Walter YAMAMOTO

IPC分类号： C08B30/18 ,  C12N9/26 ,  C12N9/44 ,  C12P19/04 ,  C12P19/16 ,  C12P19/22

CPC分类号： C08B30/18 ,  C12N9/2414 ,  C12N9/2457 ,  C12P19/04 ,  C12P19/16 ,  C12P19/22

摘要： This specification discloses process for obtaining maltodextrin having DE between 17 and 19.9 and the maltodextrins obtained from the process. The disclosed maltodextrins can be provided as a powder or in shelf stable liquid form. The disclose maltodextrins have a polysaccharide profile similar to those observed for prior art maltodextrins, but make maltodextrin solutions having a high solids content, but reduced viscosity compared to prior art maltodextrins, on equivalent solids-in-solution basis. The process combines adds an alpha-amylase and a pullulanase enzyme to a polysaccharide mixture during a saccharification step. The disclosed maltodextrins make solutions at 50° C. and greater than 65% on a solids dry solids basis having a viscosity between 5,000 and 12,000 cP and having a water activity of less than 0.80.

公开(公告)号：US11674127B2

公开(公告)日：2023-06-13

申请号：US17306001

申请日：2021-05-03

申请人： NOVOZYMES A/S

发明人： Joseph Jump ,  Nathaniel Edward Kreel ,  Bernardo Vidal, Jr. ,  Chee-Leong Soong ,  Madison Roberts ,  Melissa Carrie Hooss ,  Xinyu Shen

IPC分类号： C12N9/14 ,  C12N9/16 ,  C12N9/20 ,  C12N9/26 ,  C12N9/28 ,  C12N9/34 ,  C12N9/42 ,  C12N9/44 ,  C12N9/50 ,  C12P7/06 ,  C12P19/14 ,  C12P7/64

CPC分类号： C12N9/14 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/2457 ,  C12N9/50 ,  C12P7/06 ,  C12P19/14 ,  C12Y301/03008 ,  C12Y301/04 ,  C12Y301/04011 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01041 ,  C12Y304/21062 ,  C12Y304/23023 ,  C12Y304/24039 ,  C12P7/64 ,  Y02E50/10

摘要： A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c). Use of a protease and a phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.

公开(公告)号：US11667938B2

公开(公告)日：2023-06-06

申请号：US17465098

申请日：2021-09-02

申请人： Novozymes A/S

发明人： Shiro Fukuyama ,  Keiichi Ayabe

IPC分类号： C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

CPC分类号： C12P19/14 ,  A23L29/35 ,  C12N9/2402 ,  C12N9/2411 ,  C12N9/2428 ,  C12N9/2457 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01041 ,  C13K1/06

摘要： The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US11655311B2

公开(公告)日：2023-05-23

申请号：US16639425

申请日：2018-08-14

申请人： Corn Products Development, Inc.

发明人： Laercio Arnoni ,  Jose Bertoli ,  Walter Yamamoto

IPC分类号： C08B30/18 ,  C12P19/04 ,  C12P19/16 ,  C12P19/22 ,  C12N9/26 ,  C12N9/44

CPC分类号： C08B30/18 ,  C12N9/2414 ,  C12N9/2457 ,  C12P19/04 ,  C12P19/16 ,  C12P19/22

摘要： This specification discloses process for obtaining maltodextrin having DE between 17 and 19.9 and the maltodextrins obtained from the process. The disclosed maltodextrins can be provided as a powder or in shelf stable liquid form. The disclose maltodextrins have a polysaccharide profile similar to those observed for prior art maltodextrins, but make maltodextrin solutions having a high solids content, but reduced viscosity compared to prior art maltodextrins, on equivalent solids-in-solution basis. The process combines adds an alpha-amylase and a pullulanase enzyme to a polysaccharide mixture during a saccharification step. The disclosed maltodextrins make solutions at 50° C. and greater than 65% on a solids dry solids basis having a viscosity between 5,000 and 12,000 cP and having a water activity of less than 0.80.

公开(公告)号：US11377643B2

公开(公告)日：2022-07-05

申请号：US16617431

申请日：2018-05-31

申请人： Ultragenyx Pharmaceutical Inc.

发明人： Kiyoshi Tachikawa ,  Carlos Gustavo Perez-Garcia ,  Padmanabh Chivukula ,  Hari Bhaskaran ,  Christian W. Cobaugh ,  Sean Christopher Daugherty

IPC分类号： C12N9/10 ,  A61P3/00 ,  C12N9/44 ,  C12N15/11 ,  A61K48/00

摘要： This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), or a fragment thereof having AGL activity. The polynucleotide and oligomer molecules are expressible to provide the human AGL or a fragment thereof having AGL activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with reduced activity of amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) in a subject.

公开(公告)号：US20220186266A1

公开(公告)日：2022-06-16

申请号：US17600767

申请日：2020-04-02

申请人： Novozymes A/S

发明人： Nathaniel Edward KREEL

IPC分类号： C12P7/14 ,  C12P19/14 ,  C12N9/26 ,  C12N9/34 ,  C12N9/42 ,  C12N9/44 ,  C12P19/04 ,  C12P19/02 ,  C12P19/12

摘要： The present invention relates to processes of producing a fermentation product from starch containing material comprising (a) forming a slurry comprising the starch-containing material and water; (b) converting the starch-containing material into dextrins with an alpha-amylase; (c) saccharifying the dextrins using a carbohydrate source generating enzyme to form sugars; (d) fermenting sugars using a fermenting organism; (e) recovering the fermentation product to form whole stillage; (f) separating the whole stillage into a liquid fraction thin stillage and solid fraction wet cake; (g) hydrolyzing the thin stillage; (h) recycle a portion of the hydrolyzed thin stillage to steps (a); wherein the thin stillage in step (g) is hydrolyzed using a glucoamylase and/or polygalacturonase.

公开(公告)号：US20210395791A1

公开(公告)日：2021-12-23

申请号：US17465098

申请日：2021-09-02

申请人： Novozymes A/S

发明人： Shiro Fukuyama ,  Keiichi Ayabe

IPC分类号： C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

摘要： The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

公开(公告)号：US20210355461A1

公开(公告)日：2021-11-18

申请号：US16635474

申请日：2018-07-25

申请人： CJ CHEILJEDANG CORPORATION

发明人： Byung-Sam SON ,  Hyun Kug CHO ,  Sung Jae YANG ,  Seong Bo KIM ,  Seung Hwan KIM ,  Hyun June PARK

IPC分类号： C12N9/16 ,  C12P19/02 ,  C12N9/90 ,  C12N9/12 ,  C12N9/10 ,  C12N9/44 ,  C12N9/26 ,  C12N9/34

摘要： The present application relates to a psicose-6-phosphate phosphatase comprising motif A and motif B, a composition for producing D-psicose comprising the enzyme, and a method for producing D-psicose using the enzyme.

公开(公告)号：US11136607B2

公开(公告)日：2021-10-05

申请号：US16403919

申请日：2019-05-06

申请人： Novozymes A/S

发明人： Shiro Fukuyama ,  Keiichi Ayabe

IPC分类号： C12P19/14 ,  A23L29/30 ,  C12N9/24 ,  C12N9/26 ,  C12N9/44 ,  C12P19/02 ,  C13K1/06 ,  C12N9/34

摘要： The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.