SYSTEM AND METHOD FOR IMPROVED ANAEROBIC DIGESTION

    公开(公告)号:US20230118918A1

    公开(公告)日:2023-04-20

    申请号:US18053298

    申请日:2022-11-07

    摘要: A method for improved anaerobic digestion is presented. The method includes mixing a volume of waste material with water to form a feedstock mixture. The volume of waste material includes an initial amount of biomass and the feedstock mixture includes methanogenic bacteria either naturally present in the waste material or introduced artificially. The method also includes introducing one or more promoter substances to the feedstock mixture. The one or more promoter substances are capable of modifying the methanogenic bacteria. Modifying includes stimulating novel enzyme production in the methanogenic bacteria.

    ANAEROBIC IMMOBILIZED BACTERIAL AGENT, PREPARATION METHOD FOR SAME, AND APPLICATIONS THEREOF

    公开(公告)号:US20230063738A1

    公开(公告)日:2023-03-02

    申请号:US17794996

    申请日:2021-04-15

    申请人: TONGJI UNIVERSITY

    IPC分类号: C12P5/02 C12N11/084 C12N1/20

    摘要: Provided are an anaerobic immobilized bacterial agent, a preparation method for same, and applications thereof. The preparation method for the bacterial agent is: selecting four different anaerobic functional bacterial strains, utilizing a pure bacteria culturing technique to produce corresponding culture broths, then mixing the four culture broths according to a certain volume ratio to acquire a compound functional broth, subsequently concentrating into a functional flora precipitation, then dissolving the functional flora precipitation into a polyvinyl alcohol aqueous solution, dripping the solution into a first buffer solution to produce polyvinyl alcohol gel beads, and placing the gel beads produced into a second sulfate-containing buffer solution to produce sulfate-modified polyvinyl alcohol gel beads, that is, the anaerobic immobilized bacterial agent.

    Bioreactor insert and biofilm support, related apparatus and related methods

    公开(公告)号:US11591559B2

    公开(公告)日:2023-02-28

    申请号:US17277910

    申请日:2019-09-30

    摘要: The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to the reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

    METHODS AND COMPOSITIONS FOR PRODUCING ETHYLENE FROM RECOMBINANT MICROORGANISMS

    公开(公告)号:US20220411829A1

    公开(公告)日:2022-12-29

    申请号:US17756400

    申请日:2020-12-02

    IPC分类号: C12P5/02 C12N1/20 C12N15/52

    摘要: The present disclosure relates to recombinant microorganisms having an improved ethylene producing ability, methods of producing the same, and methods of producing ethylene. A benefit of the recombinant microorganisms and the methods disclosed herein can include increased production of ethylene from microbial cultures. An additional benefit can be the use of carbon dioxide to produce bio-ethylene useful as a feedstock for the production of plastics, textiles, and chemical materials, and for use in other applications. Another benefit of the methods and systems disclosed herein can include reduction of excess carbon dioxide from the environment.

    Devices and methods for generating electrical current from methane

    公开(公告)号:US11539066B2

    公开(公告)日:2022-12-27

    申请号:US16462701

    申请日:2017-11-22

    摘要: Methods, microbial fuel cells and microbial consortia for generating electrical current are provided according to the present invention which include providing a microbial consortium to an anode chamber of a microbial fuel cell, wherein the microbial consortium includes: 1) an engineered methanogen that contains a heterologous nucleic acid sequence encoding methyl-coenzyme M reductase derived from an anaerobic methane oxidizer, 2) an exoelectrogen microbe that produces electrically-conductive appendages and/or one or more types of electron carrier, and 3) a sludge, methane-acclimated sludge, a sludge isolate component, a methane-acclimated sludge isolate component chosen from Paracoccus spp., Geotoga spp., Geobacter spp., Methanosarcina spp., Garciella spp., humic acids; or a combination of any two or more thereof.

    Solid state fermentation reactor equipped with active support material

    公开(公告)号:US11512272B2

    公开(公告)日:2022-11-29

    申请号:US16312238

    申请日:2017-06-21

    申请人: Qvidja Kraft AB

    摘要: An active solid state fermentation bioreactor for producing gases, liquid(s) or solids from gaseous or gaseous and liquid starting materials and a fermentation process using the reactor are disclosed, The bioreactor includes three major phases; a solid phase including the porous solid support, a liquid phase comprising liquid, and a gaseous phase. The solid phase includes a porous solid support, in which at least 20% of the pore volumes have a size resulting in a liquid suction of about 0.01 to about 0.1 bars if these pores are filled with liquid, the porous solid support is inoculated with desired micro-organisms, the volume of the gaseous phase is 20% to 60% of the volume of the bioreactor, and the liquid phase is at least 20% of the reactor volume, The unsaturated capillary conductivity of filling/packing solid material of the bioreactor is at least 0.1 cm/ h. The solid state fermentation bioreactor enables a large gas-liquid interface, in which the filling material has a good capillary conductivity despite the unsaturated state.