中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

10 records (took 0.024 seconds)

    COMPOSITIONS, SYSTEMS, AND METHODS FOR DETECTING THE PRESENCE OF POLYMER SUBUNITS USING CHEMILUMINESCENCE
    1.
    发明申请
    COMPOSITIONS, SYSTEMS, AND METHODS FOR DETECTING THE PRESENCE OF POLYMER SUBUNITS USING CHEMILUMINESCENCE 审中-公开
    用于检测聚合物亚胺存在的组成，系统和方法

    公开(公告)号：WO2016040607A1

    公开(公告)日：2016-03-17

    申请号：PCT/US2015/049393

    申请日：2015-09-10

    Applicant: ILLUMINA, INC.

    Inventor： BOYANOV, Boyan ,  QIANG, Liangliang ,  GUNDERSON, Kevin, L. ,  KLAUSING, Kay ,  PICKERING, Lea ,  DE LATTRE, Cyril ,  KHURANA, Tarun

    IPC: C12Q1/68 ,  G01N33/53

    CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2535/113 ,  C12Q2535/122 ,  C12Q2563/103 ,  C12Q2563/125

    Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

    Abstract translation: 在一个方面，组合物包括底物; 耦合到所述基底的第一多核苷酸; 与第一多核苷酸杂交的第二个多核苷酸; 和与第二多核苷酸的第一个核苷酸偶联的催化剂，该催化剂可操作以使化学发光分子发射光子。 在另一方面，一种方法包括提供可操作以使第一化学发光分子发射光子的催化剂; 提供衬底; 提供耦合到所述基底的第一多核苷酸; 将第二多核苷酸与第一多核苷酸杂交; 将第一猝灭剂偶联至第二多核苷酸的第一个核苷酸; 并且通过第一猝灭剂抑制第一化学发光分子的光子发射。

    COMPOSITIONS, SYSTEMS, AND METHODS FOR DETECTING EVENTS USING TETHERS ANCHORED TO OR ADJACENT TO NANOPORES
    5.
    发明申请
    COMPOSITIONS, SYSTEMS, AND METHODS FOR DETECTING EVENTS USING TETHERS ANCHORED TO OR ADJACENT TO NANOPORES 审中-公开
    用于检测活动的组合物，系统和方法，使用锚固或邻近纳米比亚

    公开(公告)号：WO2015187670A2

    公开(公告)日：2015-12-10

    申请号：PCT/US2015/033749

    申请日：2015-06-02

    Applicant: ILLUMINA, INC.

    Inventor： MANDELL, Jeffrey, G. ,  GUNDERSON, Kevin, L. ,  GUNDLACH, Jens, H.

    IPC: C12Q1/00

    CPC classification number: C12Q1/6869 ,  G01N27/44743 ,  G01N27/44791 ,  C12Q2525/197 ,  C12Q2565/607 ,  C12Q2565/631

    Abstract: Compositions, systems, and methods for detecting events are provided. A composition can include a nanopore including a first side, a second side, and an aperture extending through the first and second sides; and a permanent tether including head and tail regions and an elongated body disposed therebetween. The head region can be anchored to or adjacent to the first or second side of the nanopore. The elongated body including a reporter region can be movable within the aperture responsive to a first event occurring adjacent to the first side of the nanopore. For example, the reporter region is translationally movable toward the first side responsive to the first event, then toward the second side, then toward the first side responsive to a second event. The first event can include adding a first nucleotide to a polynucleotide. The second event can include adding a second nucleotide to the polynucleotide.

    Abstract translation: 提供了用于检测事件的组合，系统和方法。 组合物可以包括纳米孔，其包括第一侧，第二侧和延伸穿过第一和第二侧的孔; 以及包括头部和尾部区域的永久系绳以及设置在它们之间的细长体。 头部区域可以锚固到或邻近纳米孔的第一或第二侧。 包括报告器区域的细长体可响应于邻近纳米孔第一侧发生的第一事件而在孔内可移动。 例如，响应于第一事件，然后朝向第二侧，然后响应于第二事件朝向第一侧，报告器区域可向第一侧平移移动。 第一个事件可以包括向多核苷酸添加第一个核苷酸。 第二个事件可以包括向多核苷酸添加第二个核苷酸。

    COMPOSITIONS, SYSTEMS, AND METHODS FOR SEQUENCING POLYNUCLEOTIDES USING TETHERS ANCHORED TO POLYMERASES ADJACENT TO NANOPORES
    7.
    发明申请
    COMPOSITIONS, SYSTEMS, AND METHODS FOR SEQUENCING POLYNUCLEOTIDES USING TETHERS ANCHORED TO POLYMERASES ADJACENT TO NANOPORES 审中-公开
    组合物，系统和方法，用于使用粘附到与纳米多面相似的聚合物的四聚体来测序多核苷酸

    公开(公告)号：WO2016196755A1

    公开(公告)日：2016-12-08

    申请号：PCT/US2016/035457

    申请日：2016-06-02

    Applicant: ILLUMINA, INC.

    Inventor： GUNDERSON, Kevin, L. ,  MANDELL, Jeffrey, G.

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  G01N33/48721 ,  C12Q2521/543 ,  C12Q2525/197 ,  C12Q2565/607 ,  C12Q2565/631

    Abstract: A composition includes a nanopore including first and second sides and an aperture, nucleotides each including an elongated tag, and a first polynucleotide that is complementary to a second polynucleotide. A polymerase can be disposed adjacent to the first side of the nanopore and configured to add nucleotides to the first polynucleotide based on a sequence of the second polynucleotide. A permanent tether can include a head region anchored to the polymerase, a tail region, and an elongated body disposed therebetween that occurs in the aperture of the nanopore. A first moiety can be disposed on the elongated body that binds to the elongated tag of a first nucleotide upon which the polymerase is acting. A reporter region can be disposed on the elongated body that indicates when the first nucleotide is complementary or is not complementary to a next nucleotide in the sequence of the second polynucleotide.

    Abstract translation: 组合物包括包含第一和第二侧面的纳米孔和一个孔，各自包含细长标签的核苷酸和与第二多核苷酸互补的第一多核苷酸。 可以将聚合酶设置在与纳米孔的第一侧相邻的位置，并配置为基于第二多核苷酸的序列向第一多核苷酸添加核苷酸。 永久系绳可以包括锚定到聚合酶的头部区域，尾部区域和设置在其间的细长体，其发生在纳米孔的孔中。 第一部分可以设置在细长体上，其结合聚合酶作用的第一核苷酸的细长标签。 报告区可以放置在细长体上，其指示第一个核苷酸何时互补或不与第二个多核苷酸的序列中的下一个核苷酸互补。

Patent Agency Ranking