Abstract:
Die vorliegende Erfindung betrifft eine Vorrichtung zum Trennen und/oder Isolieren von Substanzen in bzw. aus einem Gemisch mit verbesserter Ausnutzung der Kapazität chromatographischer Medien, wobei die Vorrichtung ein erstes Chromatographiesystem, eine dem ersten Chromatographiesystem nachgeschaltete zweite Chromatographiematrix und einen Sensor zur Detektion der im Fluid enthaltenden Substanzen umfasst. Darüber hinaus betrifft die vorliegende Erfindung sowohl die Verwendung dieser Vorrichtung als auch ein Verfahren zum Trennen und/oder Isolieren von Substanzen in bzw. aus einem Gemisch in einem Fluid.
Abstract:
A sample separation apparatus (100, 200) for separating a fluidic sample (104), the sample separation apparatus (100, 200) comprising: a first separation unit (102) for separating the fluidic sample (104); a first fluid drive (106) configured for generating a fluid flow for conducting the fluidic sample (104) to be separated through the first separation unit (102); a second separation unit (110), arranged downstream of the first separation unit (102), for further separating the fluidic sample (104) after treatment by the first separation unit (102); a second fluid drive (112) configured for generating a fluid flow for conducting the fluidic sample (104) or at least parts thereof, after treatment by the first separation unit (102), through the second separation unit (110); a fluidic valve (114) having a first inlet (116) fluidically coupled to one of the first fluid drive (106) and the second fluid drive (112); the fluidic valve (114) having a second inlet (118) fluidically coupled to the other of the first fluid drive (106) and the second fluid drive (112); the fluidic valve (114) comprising at least two different sets (131, 133) of storage paths, wherein each set of storage paths comprises a first storage path (128); and wherein the first storage path (128) of a first set (131) of said at least two sets of storage paths has a first volume and the first storage path (132) of a second set (133) of said at least two sets of storage paths has a second volume different from the first volume; the fluidic valve (114) being configured for selectively switching one set of said least two sets of storage paths to the first inlet (116) and the second inlet (118).
Abstract:
An apparatus for coupling a liquid chromatography column comprising an end fitting to a tubing comprises: at least one body member comprising at least one chamber; a first spring within the at least one chamber, the first spring configured so as to apply a first force to the tubing towards the column end fitting; a second spring within the at least one chamber, the second spring configured to apply a second force to a deformable sealing member towards the column end fitting; and a pushing and latching mechanism configured to push the at least one body member and the first and second springs towards the column end fitting.
Abstract:
A chromatography system with a main column (1) comprising a chromatography resin, a first guard column (2) and a second guard column (3), wherein the first guard column (2) is connected to a first end of the main column (4), the second guard column (3) is connected to a second end (5) of the main column and the bed volumes of said first and second guard columns are each less than about 50% of the bed volume of the main column.
Abstract:
A sample plate, portable analysis apparatus and method of analysing sulphur and/or nitrogen compounds in a sample fluid, the method comprising feeding a sample fluid to a sample plate having a sample inlet, a reaction zone, an analysis zone, and at least one separation zone, the sample plate being adapted to allow; (a) a sample fluid to be fed to the sample plate through the inlet to the reaction zone or optionally to a separation zone, which separation zone separates the sample fluid into two or more fractions at least one of which is fed to the reaction zone; (b) a reactant to be fed to the reaction zone; (c) the reaction zone to be maintained under conditions that enable reaction to occur between the reactant and the sample fluid or fraction thereof to produce a product fluid; and (d) transfer of the product fluid to the analysis zone or optionally to a separation zone in which the product fluid is separated into two or more fractions, at least one of which is transferred to the analysis zone.
Abstract:
The present invention relates to methods and compositions for the identification of cancer markers. In particular, the present invention provides methods and compositions for the identification of glycosylated proteins and protein glycosylation patterns. The present invention further provides cancer markers identified using the described methods.
Abstract:
A process for obtaining one or more than one salt of an organic acid(s), or organic acid(s), from an aqueous sugar stream comprising one or more than one mineral acid and the organic acid(s) is provided. The process comprises introducing the aqueous sugar stream to a separation system comprising one or more beds of anion exchange resin and obtaining a stream therefrom comprising the sugar. The one or more beds of anion exchange resin are then regenerated in one or more stages to produce at least one product stream comprising the organic acid, a salt of the organic acid, or a combination thereof, and a separate outlet stream comprising the mineral acid, a salt of the mineral acid, or a combination thereof. The product stream is then recovered. The separation may be conducted with two separation units, or using a single anion exchange unit.
Abstract:
The present invention relates to methods and compositions for the identification of cancer markers. In particular, the present invention provides methods and compositions for the identification of glycosylated proteins and protein glycosylation patterns. The present invention further provides cancer markers identified using the described methods.