PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING LECTIN PROTEIN KINASE (LPK) POLYPEPTIDES AND HOMOLOGS THEREOF
    91.
    发明申请
    PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING LECTIN PROTEIN KINASE (LPK) POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开
    具有改变的根构架的植物,涉及编码赖氨酸蛋白激酶(LPK)多肽的基因的相关构建体和方法及其同源物

    公开(公告)号:WO2011097215A2

    公开(公告)日:2011-08-11

    申请号:PCT/US2011/023319

    申请日:2011-02-01

    CPC classification number: C12N15/8241

    Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.

    Abstract translation: 分离的多核苷酸和多肽以及特别用于改变植物根部结构的重组DNA构建体,包含这些重组DNA构建体的组合物(如植物或种子),以及利用这些重组DNA构建体的方法。 重组DNA构建体包含与在植物中有功能的启动子可操作连接的多核苷酸,其中所述多核苷酸编码可用于改变植物根结构的多肽。

    A METHOD FOR THE TRANSIENT EXPRESSION OF NUCLEIC ACIDS IN PLANTS
    93.
    发明申请
    A METHOD FOR THE TRANSIENT EXPRESSION OF NUCLEIC ACIDS IN PLANTS 审中-公开
    一种植物中核酸瞬时表达的方法

    公开(公告)号:WO2010144775A1

    公开(公告)日:2010-12-16

    申请号:PCT/US2010/038271

    申请日:2010-06-11

    CPC classification number: C12N15/8241 C12N15/8216

    Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.

    Abstract translation: 提供了在植物中瞬时表达蛋白质的组合物和方法。 组合物包含植物,种子,植物组织和表达蛋白质的植物部分,其中蛋白质被瞬时表达,并且蛋白质的瞬时表达可以用作如何在稳定的转基因植物中表达所述蛋白质的预测模型 定性和定量数据。 可以使用预测模型,但不限于:启动子评估,用于最佳性能的表达盒构建的评价(例如增加增强子或基因沉默抑制子的添加),表达异源基因的最佳方式的评估(例如点突变,靶向), 内源基因敲除的快速评价,蛋白质表达水平的评价,细胞靶向,组织靶向,转录增强子,翻译增强子蛋白毒性和代谢分析。 进一步提供使用的方法。

    METHODS OF AFFECTING PLANT GROWTH WITH MICRORNA
    100.
    发明申请
    METHODS OF AFFECTING PLANT GROWTH WITH MICRORNA 审中-公开
    用微生物影响植物生长的方法

    公开(公告)号:WO2008115487A1

    公开(公告)日:2008-09-25

    申请号:PCT/US2008/003526

    申请日:2008-03-17

    Abstract: Provided herein are compositions and methods for producing transgenic plants. In specific embodiments, transgenic plants comprise a construct comprising a polynucleotide encoding microRNA167 (miR167), or precursor thereof, operably linked to a plant pericycle- specific promote, wherein the miR167 is ectopically overexpressed in the transgenic plants, and wherein the promoter is optionally a constitutive or inducible promoter. In some embodiments, the transgenic plant has an improved agronomic or nutritional characteristic when cultivated in nitrogen-rich conditions as compared to a wild type plant cultivated in the same conditions. Also provided herein are commercial products (e.g., pulp, paper, paper products, or lumber) derived from the transgenic plants (e.g., transgenic trees) produced using the methods provided herein.

    Abstract translation: 本文提供了用于产生转基因植物的组合物和方法。 在具体实施方案中,转基因植物包含编码microRNA167(miR167)或其前体的多核苷酸的构建体,其可操作地连接到植物周期特异性促进剂,其中所述miR167在转基因植物中异位表达,并且其中所述启动子任选为 组成型或诱导型启动子。 在一些实施方案中,与在相同条件下培养的野生型植物相比,当在富氮条件下培养时,转基因植物具有改善的农学或营养特性。 本文还提供了使用本文提供的方法产生的转基因植物(例如,转基因树)的商业产品(例如纸浆,纸,纸制品或木材)。

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