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公开(公告)号：WO2013019411A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/047202
申请日：2012-07-18
Applicant: E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL , CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
Inventor： CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
CPC classification number: C12N15/8213
Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
Abstract translation: 提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。 方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。 一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。
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公开(公告)号：WO2012129373A2
公开(公告)日：2012-09-27
申请号：PCT/US2012/030061
申请日：2012-03-22
Applicant: PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , LASSNER, Michael , JANTZ, Derek , SMITH, James, Jefferson , CIGAN, Mark , FALCO, Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-bin , SVITASHEV, Sergei
Inventor： LASSNER, Michael , JANTZ, Derek , SMITH, James, Jefferson , CIGAN, Mark , FALCO, Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-bin , SVITASHEV, Sergei
CPC classification number: C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
Abstract translation: 公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。 所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。 还公开了植物中的复杂的转基因性状基因座。 复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。 还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。
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公开(公告)号：WO2013066423A3
公开(公告)日：2013-09-12
申请号：PCT/US2012043082
申请日：2012-06-19
Applicant: PIONEER HI BRED INT , DU PONT , BIDNEY DENNIS L , CIGAN MARK , FALCO CARL , GAO HUIRONG , JANTZ DEREK , LASSNER MIKE , LOWE KEITH , LYZNIK LESZEK ALEKSANDER , SMITH JAMES JEFFERSON
Inventor： BIDNEY DENNIS L , CIGAN MARK , FALCO CARL , GAO HUIRONG , JANTZ DEREK , LASSNER MIKE , LOWE KEITH , LYZNIK LESZEK ALEKSANDER , SMITH JAMES JEFFERSON
IPC: C12N15/82
CPC classification number: C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand- break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
Abstract translation: 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一种靶向修饰的雄性育性基因的核酸分子，编码作为工程化双链断裂诱导剂和表达盒的核酸内切酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：WO2013066423A2
公开(公告)日：2013-05-10
申请号：PCT/US2012/043082
申请日：2012-06-19
Applicant: PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , BIDNEY, Dennis, L. , CIGAN, Mark , FALCO, Carl , GAO, Huirong , JANTZ, Derek , LASSNER, Mike , LOWE, Keith , LYZNIK, Leszek, Aleksander , SMITH, James, Jefferson
Inventor： BIDNEY, Dennis, L. , CIGAN, Mark , FALCO, Carl , GAO, Huirong , JANTZ, Derek , LASSNER, Mike , LOWE, Keith , LYZNIK, Leszek, Aleksander , SMITH, James, Jefferson
IPC: C12N15/82
CPC classification number: C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a eel! comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand- break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
Abstract translation: 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定鳗鱼！ 包括目标序列的改变。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一种靶向修饰的雄性育性基因的核酸分子，编码作为工程化双链断裂诱导剂和表达盒的核酸内切酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：WO2005037863A9
公开(公告)日：2005-06-23
申请号：PCT/US2004031241
申请日：2004-09-23
Applicant: PIONEER HI BRED INT , LYZNIK L ALEXANDER , GORDON-KAMM WILLIAM J , GAO HUIRONG
Inventor： LYZNIK L ALEXANDER , GORDON-KAMM WILLIAM J , GAO HUIRONG
IPC: A01H1/00 , A01H5/00 , C07K14/415 , C12N5/04 , C12N15/82
CPC classification number: C12N15/827 , C07K14/415 , C12N15/8201 , C12N15/8209 , C12N15/8216 , C12N15/8245 , C12N15/8261 , C12N15/8267 , C12N15/8271 , C12N15/8291 , Y02A40/146
Abstract: The invention provides isolated polynucleotides and their encoded proteins that are involved in splicing or modulating splicing activity. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering splicing protein content and/or composition of plants.
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Patent Agency Ranking
公开(公告)号：WO2009006297A3
公开(公告)日：2009-02-19
申请号：PCT/US2008068570
申请日：2008-06-27
Applicant: PIONEER HI BRED INT , LYZNIK L ALEKSANDER , TAO YUMIN , GAO HUIRONG
Inventor： LYZNIK L ALEKSANDER , TAO YUMIN , GAO HUIRONG
CPC classification number: C12N15/8201 , A01H1/02 , C12N9/22 , C12N15/8213 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
Abstract: Methods and compositions for altering the genome of a monocot plant cell, and a monocot plant are disclosed. The methods and compositions use a double-strand break inducing agent to alter a monocot plant or plant cell genomic sequence comprising a recognition sequence for the double-strand break inducing agent.
Abstract translation: 公开了用于改变单子叶植物细胞和单子叶植物的基因组的方法和组合物。 所述方法和组合物使用双链断裂诱导剂来改变包含双链断裂诱导剂的识别序列的单子叶植物或植物细胞基因组序列。
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公开(公告)号：WO2009006297A2
公开(公告)日：2009-01-08
申请号：PCT/US2008/068570
申请日：2008-06-27
Applicant: PIONEER HI-BRED INTERNATIONAL, INC. , LYZNIK, L., Aleksander , TAO, Yumin , GAO, Huirong
Inventor： LYZNIK, L., Aleksander , TAO, Yumin , GAO, Huirong
CPC classification number: C12N15/8201 , A01H1/02 , C12N9/22 , C12N15/8213 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
Abstract: Methods and compositions for altering the genome of a monocot plant cell, and a monocot plant are disclosed. The methods and compositions use a double-strand break inducing agent to alter a monocot plant or plant cell genomic sequence comprising a recognition sequence for the double-strand break inducing agent.
Abstract translation: 公开了用于改变单子叶植物细胞和单子叶植物基因组的基因组的方法和组合物。 该方法和组合物使用双链断裂诱导剂来改变包含双链断裂诱导剂的识别序列的单子叶植物或植物细胞基因组序列。
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8.发明申请ALTERNATIVE SPLICING FACTORS POLYNUCLEOTIDES, POLYPEPTIDES AND USES THEREOF 审中-公开选择性刺入因子多核苷酸，多肽及其用途
公开(公告)号：WO2005037863A3
公开(公告)日：2005-08-04
申请号：PCT/US2004031241
申请日：2004-09-23
Applicant: PIONEER HI BRED INT , LYZNIK L ALEXANDER , GORDON-KAMM WILLIAM J , GAO HUIRONG
Inventor： LYZNIK L ALEXANDER , GORDON-KAMM WILLIAM J , GAO HUIRONG
IPC: A01H1/00 , A01H5/00 , C07K14/415 , C12N5/04 , C12N15/82
CPC classification number: C12N15/827 , C07K14/415 , C12N15/8201 , C12N15/8209 , C12N15/8216 , C12N15/8245 , C12N15/8261 , C12N15/8267 , C12N15/8271 , C12N15/8291 , Y02A40/146
Abstract: The invention provides isolated polynucleotides and their encoded proteins that are involved in splicing or modulating splicing activity. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering splicing protein content and/or composition of plants.
Abstract translation: 本发明提供了分离的多核苷酸及其编码的参与剪接或调节剪接活性的蛋白质。 本发明进一步提供重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了涉及改变剪接蛋白含量和/或植物组成的方法和组合物。
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公开(公告)号：WO2013066423A8
公开(公告)日：2014-07-10
申请号：PCT/US2012043082
申请日：2012-06-19
Applicant: PIONEER HI BRED INT , DU PONT , BIDNEY DENNIS L , CIGAN MARK , FALCO CARL , GAO HUIRONG , JANTZ DEREK , LASSNER MIKE , LOWE KEITH , LYZNIK LESZEK ALEKSANDER , SMITH JAMES JEFFERSON
Inventor： BIDNEY DENNIS L , CIGAN MARK , FALCO CARL , GAO HUIRONG , JANTZ DEREK , LASSNER MIKE , LOWE KEITH , LYZNIK LESZEK ALEKSANDER , SMITH JAMES JEFFERSON
IPC: C12N15/82
CPC classification number: C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand- break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
Abstract translation: 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一种靶向修饰的雄性育性基因的核酸分子，编码作为工程化双链断裂诱导剂和表达盒的核酸内切酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：WO2012129373A3
公开(公告)日：2013-03-14
申请号：PCT/US2012030061
申请日：2012-03-22
Applicant: PIONEER HI BRED INT , DU PONT , LASSNER MICHAEL , JANTZ DEREK , SMITH JAMES JEFFERSON , CIGAN MARK , FALCO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , SVITASHEV SERGEI
Inventor： LASSNER MICHAEL , JANTZ DEREK , SMITH JAMES JEFFERSON , CIGAN MARK , FALCO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , SVITASHEV SERGEI
CPC classification number: C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
Abstract translation: 公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。 所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。 还公开了植物中的复杂的转基因性状基因座。 复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。 还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。
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