公开(公告)号：WO2008005470A2

公开(公告)日：2008-01-10

申请号：PCT/US2007015425

申请日：2007-06-29

Applicant: UNIV PENNSYLVANIA ,  SLUSKY JOANNA S ,  YIN HANG ,  DEGRADO WILLIAM F

Inventor： SLUSKY JOANNA S ,  YIN HANG ,  DEGRADO WILLIAM F

IPC: A61K38/16

CPC classification number: C07K14/001 ,  A61K38/00 ,  C07K16/2848 ,  C07K2317/34 ,  C07K2317/76 ,  G01N33/6845 ,  G01N33/6872 ,  G01N2500/04

Abstract: Polypeptides which bind to the helical transmembrane region of membrane proteins are disclosed, as are methods for the design of polypeptides that bind to the transmembrane region of membrane proteins. Also provided are methods for the use of the disclosed polypeptides in various applications, as well as products made through the practice of the instant methods.

Abstract translation: 公开了与膜蛋白的螺旋跨膜区结合的多肽，以及用于设计与膜蛋白的跨膜区结合的多肽的方法。 还提供了在各种应用中使用所公开的多肽的方法，以及通过实施本方法制备的产品。

公开(公告)号：WO2007146229A2

公开(公告)日：2007-12-21

申请号：PCT/US2007/013688

申请日：2007-06-07

Applicant: TETHYS BIOSCIENCE, INC. ,  URDEA, Mikey ,  MCKENNA, Michael ,  ARENSDORF, Pat

Inventor： URDEA, Mikey ,  MCKENNA, Michael ,  ARENSDORF, Pat

IPC: G01N33/68 ,  G01N33/74

CPC classification number: G01N33/6893 ,  G01N33/6845 ,  G01N33/74 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/325 ,  G01N2800/50 ,  G01N2800/52 ,  G06F19/00 ,  G16H50/30 ,  Y02A90/24 ,  Y02A90/26

Abstract: Disclosed are methods of identifying subjects with arteriovascular disease, subjects at risk for developing arteriovascular disease, methods of differentially diagnosing diseases associated with arteriovascular disease from other diseases or withiin sub-classifications of arteriovascular disease, methods of evaluating the risk of arteriovascular events in patients with arteriovascular disease, methods of evaluatinig the effectiveness of treatments in subjects with arteriovascular disease, and methods of selecting therapies for treating arteriovascular disease.

Abstract translation: 公开了确定具有动脉血管疾病的受试者，处于发展中的动脉血管疾病风险的受试者的方法，与其他疾病相关的血管疾病相关疾病或血管疾病亚型分类的方法，评估患者的血管事件风险的方法 动脉血管疾病，评估治疗在动脉血管疾病患者中的有效性的方法，以及选择用于治疗动脉血管疾病的疗法的方法。

公开(公告)号：WO2007106456A2

公开(公告)日：2007-09-20

申请号：PCT/US2007/006256

申请日：2007-03-12

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  WEHRMAN, Tom ,  BLAU, Helen M.

Inventor： WEHRMAN, Tom ,  BLAU, Helen M.

IPC: C12Q1/68

CPC classification number: C12Q1/34 ,  C12N15/1055 ,  G01N33/6845

Abstract: Methods and compositions for detecting molecular interactions are provided. Aspects of the invention include the use of a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods.

Abstract translation: 提供了检测分子相互作用的方法和组合物。 本发明的方面包括使用还原亲和力酶互补报告体系。 还提供了用于实施方法实施例的系统和试剂盒。

公开(公告)号：WO2007076339A2

公开(公告)日：2007-07-05

申请号：PCT/US2006/062276

申请日：2006-12-19

Applicant: GENERAL ELECTRIC COMPANY ,  WOOD, Nichole Lea ,  AMARATUNGA, Mohan Mark ,  GRADE, Hans ,  SYUD, Faisal Ahmed ,  SOOD, Anup ,  SMITH, Reginald Donovan ,  DINC, Ayse Betul ,  WILLIAMS, Amy Casey ,  MURRAY, Anthony John ,  GODDARD, Gregory Daryll

Inventor： WOOD, Nichole Lea ,  AMARATUNGA, Mohan Mark ,  GRADE, Hans ,  SYUD, Faisal Ahmed ,  SOOD, Anup ,  SMITH, Reginald Donovan ,  DINC, Ayse Betul ,  WILLIAMS, Amy Casey ,  MURRAY, Anthony John ,  GODDARD, Gregory Daryll

IPC: G01N33/68

CPC classification number: G01N33/6845 ,  G01N33/6848

Abstract: Agents and methods for qualitative and quantitative analysis a protein complex or protein complexes using isotope-labeled symmetrical bifunctional crosslinkers and mass spectrometry are provided. Targeting moieties, cell permeability moieties, or affinity moieties, may be appended to the bifunctional crosslinkers. The isotope-labeled symmetrical bifunctional crosslinkers may be used in a kit or as a library.

Abstract translation: 提供了使用同位素标记的对称双功能交联剂和质谱定性和定量分析蛋白质复合物或蛋白质复合物的试剂和方法。 靶向部分，细胞渗透性部分或亲和部分可附加到双功能交联剂上。 同位素标记的对称双功能交联剂可用于试剂盒或文库。

公开(公告)号：WO2007009457A3

公开(公告)日：2007-05-24

申请号：PCT/DK2006000410

申请日：2006-07-12

Applicant: 2CUREX APS ,  THASTRUP OLE ,  HAGEL GRITH ,  ST HILAIRE PHAEDRIA MARIE ,  NORRILD JENS CHR

Inventor： THASTRUP OLE ,  HAGEL GRITH ,  ST HILAIRE PHAEDRIA MARIE ,  NORRILD JENS CHR

IPC: G01N33/68 ,  G01N33/543

CPC classification number: G01N33/6842 ,  G01N33/6845 ,  G01N2500/00

Abstract: The present invention relates to a method for identifying protein ligands capable of modulating a cellular response. The method furthermore enables identification of the protein(s) binding to the identified ligand. The method may in particular be useful in drug screening processes and allows screening for biological activity and for drug targets in a high through-put screening format.

Abstract translation: 本发明涉及鉴定能调节细胞反应的蛋白质配体的方法。 该方法还能够鉴定与所鉴定的配体结合的蛋白质。 该方法特别可用于药物筛选过程，并允许以高通量筛选形式筛选生物活性和药物靶标。

公开(公告)号：WO2006036688A9

公开(公告)日：2007-05-03

申请号：PCT/US2005033795

申请日：2005-09-21

Applicant: ARENA PHARM INC ,  LEONARD JAMES N ,  HAKAK YARON

Inventor： LEONARD JAMES N ,  HAKAK YARON

IPC: G01N33/68

CPC classification number: G01N33/566 ,  G01N33/6845 ,  G01N2333/726 ,  G01N2500/00

Abstract: The present invention relates to a method for identifying a metabolic stabilizing compound by: a) contacting a candidate compound with GPR43, and b) determining whether GPR43 functionality is modulated, where a modulation in GPR43 functionality is indicative of the candidate compound being a metabolic stabilizing compound. In addition, the invention relates to a method for identifying a metabolic stabilizing compound, comprising: a) contacting a candidate compound with GPR43, and b) determining whether GPR43 functionality is increased, wherein an increase in GPR43 functionality is indicative of the candidate compound being a metabolic stabilizing compound. Further, the invention relates to a method for identifying a metabolic stabilizing compound, comprising: a) contacting a candidate compound with GPR43, and b) determining whether GPR43 functionality is decreased, wherein a decrease in GPR43 functionality is indicative of the candidate compound being a metabolic stabilizing compound.

Abstract translation: 本发明涉及通过以下方式鉴定代谢稳定化合物的方法：a）使候选化合物与GPR43接触，和b）确定是否调节GPR43功能性，其中GPR43功能性的调节指示候选化合物是代谢稳定剂 复合。 此外，本发明涉及用于鉴定代谢稳定化合物的方法，其包括：a）使候选化合物与GPR43接触，和b）确定GPR43的功能是否增加，其中GPR43功能的增加指示候选化合物为 代谢稳定化合物。 此外，本发明涉及用于鉴定代谢稳定化合物的方法，包括：a）使候选化合物与GPR43接触，以及b）确定GPR43的功能性是否降低，其中GPR43功能的降低指示候选化合物为 代谢稳定化合物。

公开(公告)号：WO2004015061A3

公开(公告)日：2007-04-26

申请号：PCT/US0314743

申请日：2003-05-13

Applicant: AUTOMATED CELL INC ,  SACHSENMEIER KRIS F ,  KOEBLER DOUGLAS ,  SABOL DARRIN ,  BAHNSON ALFRED B ,  HOUCK RAMOND K

Inventor： SACHSENMEIER KRIS F ,  KOEBLER DOUGLAS ,  SABOL DARRIN ,  BAHNSON ALFRED B ,  HOUCK RAMOND K

IPC: G01N33/48 ,  G01N33/50 ,  G01N33/68

CPC classification number: G01N33/6845 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/5047 ,  G01N33/68 ,  G01N2500/10

Abstract: For purposes of determining the function of a protein, an automated system captures images of cells, each cell located in a predetermined well. After a given cell is exposed to a protein of interest, the system measures the responses of the cell over time, evaluating a variety of cellular parameters. Analytical software within the system evaluates data generated by these measurements, at single-cell resolution. By comparing with various controls the data thus obtained, the system illuminates the function of a protein with respect to one or more disease models, independent of information regarding the structure, chemistry or underlying genomics of the protein.

Abstract translation: 为了确定蛋白质的功能，自动化系统捕获细胞的图像，每个细胞位于预定的孔中。 在给定细胞暴露于感兴趣的蛋白质之后，系统测量细胞随时间的响应，评估各种细胞参数。 系统内的分析软件以单细胞分辨率评估由这些测量产生的数据。 通过与各种对照相比较获得的数据，该系统相对于一种或多种疾病模型照射蛋白质的功能，与关于蛋白质的结构，化学或潜在基因组学的信息无关。

公开(公告)号：WO2006085984A3

公开(公告)日：2007-02-22

申请号：PCT/US2005024491

申请日：2005-07-11

Applicant: AMAOX INC ,  SMITH MILTON G ,  CRAWFORD KRITH D

Inventor： SMITH MILTON G ,  CRAWFORD KRITH D

IPC: G01N33/68 ,  C07K1/04 ,  G01N33/53 ,  G06F19/00

CPC classification number: G01N33/6842 ,  G01N33/5091 ,  G01N33/574 ,  G01N33/6845 ,  G01N33/6893 ,  Y02A90/24 ,  Y02A90/26

Abstract: The present invention relates to immunological cells that are useful in detecting changes in physiological states, which provide for methods of diagnosing diseases or monitoring the course of patient therapy. Also provided are arrays of antigen presenting cell-specific markers for detecting changes in physiological states, and methods of detecting such changes.

Abstract translation: 本发明涉及可用于检测生理状态变化的免疫细胞，其提供诊断疾病或监测患者治疗过程的方法。 还提供了用于检测生理状态变化的抗原呈递细胞特异性标志物的阵列，以及检测这些变化的方法。

公开(公告)号：WO2006062882A3

公开(公告)日：2006-11-02

申请号：PCT/US2005043874

申请日：2005-12-03

Applicant: UNIV CALIFORNIA LOS ALAMOS NAT

Inventor： WALDO GEOFFREY S ,  CABANTOUS STEPHANIE

IPC: C12Q1/66 ,  C12N15/63

CPC classification number: C40B30/04 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6845

Abstract: The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (ß-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (ß-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GPF amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

Abstract translation: 本发明提供基于荧光和发色蛋白的工程化片段的蛋白质标记和相互作用检测系统，其需要融合的相互作用多肽来驱动片段的缔合，并且进一步是可溶的和稳定的，并且不改变它们所针对的多肽的溶解度 融合。 在一个实施方案中，测试蛋白X与GFP的十六个氨基酸片段（β-链10，氨基酸198-214）融合，工程改造为不干扰融合蛋白溶解性。 第二种测试蛋白Y与GFP的16个氨基酸片段（β-链11，氨基酸215-230）融合，工程改造为不干扰融合蛋白的溶解性。 当X和Y相互作用时，它们使GFP链接近，并通过与由GPF氨基酸1-198（链1-9）组成的第三个GFP片段互补来检测。 当GFP链10和11通过蛋白X和Y的相互作用结合在一起时，它们与GFP链1-9自发缔合，导致结构互补，折叠和伴随的GFP荧光。

公开(公告)号：WO2006099747A1

公开(公告)日：2006-09-28

申请号：PCT/CA2006/000451

申请日：2006-03-24

Applicant: NATIONAL RESEARCH COUNCIL OF CANADA ,  TANHA, Jamshid

Inventor： TANHA, Jamshid

IPC: C12N15/13 ,  C07K1/00 ,  A61K39/395 ,  C07K14/52 ,  C07K14/705 ,  C07K14/725 ,  C07K16/00 ,  C12N1/11 ,  C12N15/70 ,  C12Q1/70 ,  C40B30/00 ,  C40B40/02 ,  C40B40/10 ,  G01N33/50

CPC classification number: C07K16/005 ,  C07K16/1271 ,  C07K16/1282 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/92 ,  C07K2317/94 ,  C12N15/1037 ,  G01N33/6845

Abstract: Polypeptides with desirable biophysical properties such as solubility, stability, high expression, monomericity, binding specificity or non-aggregation, including monomeric human VHs and VLs, are identified using a high throughput method for screening polypeptides, comprising the steps of obtaining a phage display library, allowing infection of a bacterial lawn by the library phage, and identifying phage which form larger than average plaques on the bacterial lawn. Sequences of monomeric human VHs and VLs are identified, which may be useful for immunotherapy or as diagnostic agents. Multimer complexes of human VHs and VLs are also identified. The VHs and VLs identified may be used to create further libraries for identifying additional polypeptides. Further, the VHs and VLs may be subjected to DNA shuffling to select for improved biophysical properties.

Abstract translation: 使用高通量方法鉴定多肽，其具有所需的生物物理性质如溶解度，稳定性，高表达，单体性，结合特异性或非聚集，包括单体人VH和VL，其包括以下步骤：获得噬菌体展示文库 ，允许文库噬菌体感染细菌草坪，并鉴定形成比细菌草坪上的平均斑块大的噬菌体。 确定单体人VH和VL的序列，其可用于免疫治疗或用作诊断剂。 还鉴定了人VH和VL的多聚体复合物。 鉴定的VH和VL可用于产生用于鉴定另外的多肽的另外的文库。 此外，可以对VH和VL进行DNA改组以选择改善的生物物理性质。