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    HYBRIDIZATION-BASED BIOSENSOR CONTAINING HAIRPIN PROBES AND USE THEREOF
    11.
    发明申请
    HYBRIDIZATION-BASED BIOSENSOR CONTAINING HAIRPIN PROBES AND USE THEREOF 审中-公开
    基于混合的生物传感器包含毛发探针及其用途

    公开(公告)号：WO2004061127A2

    公开(公告)日：2004-07-22

    申请号：PCT/US2004/000093

    申请日：2004-01-02

    Applicant: UNIVERSITY OF ROCHESTER ,  MILLER, Benjamin, L. ,  KRAUSS, Todd, D. ,  DU, Hui ,  CRNKOVICH, Nicole ,  STROHSAHL, Christopher, M.

    Inventor： MILLER, Benjamin, L. ,  KRAUSS, Todd, D. ,  DU, Hui ,  CRNKOVICH, Nicole ,  STROHSAHL, Christopher, M.

    IPC: C12Q

    CPC classification number: G01N21/6486 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/689 ,  C12Q1/6893 ,  C12Q1/70 ,  Y02A50/55 ,  Y02A50/60 ,  C12Q2525/301 ,  C12Q2565/107 ,  C12Q2565/101 ,  C12Q2565/607

    Abstract: A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, a first region, and a second region complementary to the first region, the nucleic acid probe having, under appropriate conditions, either a hairpin conformation with the first and second regions hybridized together or a non-haipin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in the non-harpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their mehtods of use are also disclosed.

    Abstract translation: 一种传感器芯片，包括：荧光淬火表面; 核酸探针，其含有具有与荧光猝灭表面结合的第一末端的第一和第二末端，第一区域和与第一区域互补的第二区域，核酸探针在适当条件下具有与 第一和第二区域一起杂交或非海波素构象; 和与第一核酸分子的第二末端结合的第一荧光团。 当第一核酸分子处于发夹构象时，荧光淬灭表面基本上淬灭第一荧光团的荧光发射; 并且当第一核酸分子处于非harpin构象时，荧光团的荧光发射基本上不被荧光淬灭表面骤冷。 还公开了各种核酸探针，制造传感器芯片的方法，包含传感器芯片的生物传感器装置及其使用方法。

    METHOD FOR IN VITRO CULTURE OF VIRUSES OF THE TOGAVIRIDAE AND FLAVIVIRIDAE FAMILY AND USES
    14.
    发明申请
    METHOD FOR IN VITRO CULTURE OF VIRUSES OF THE TOGAVIRIDAE AND FLAVIVIRIDAE FAMILY AND USES 审中-公开
    花粉虱病毒和禽流感病毒及其用途的病毒体外培养方法

    公开(公告)号：WO01009289A1

    公开(公告)日：2001-02-08

    申请号：PCT/FR2000/002202

    申请日：2000-07-31

    IPC: A61K39/12 ,  A61P31/14 ,  C07K16/08 ,  C12N5/02 ,  C12N7/00 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/70 ,  C12R1/93 ,  G01N33/569 ,  C12N5/10 ,  C12N5/06

    CPC classification number: G01N33/56983 ,  C12N7/00 ,  C12N2770/24251 ,  G01N2333/18 ,  Y02A50/60

    Abstract: The invention concerns a method of culturing, propagating and replicating in vitro viruses of the Togaviridae and Flaviviridae families which consists in: providing at least a fraction of LVP's obtained from serum or plasma of a patient infected with at least a virus of the Togaviridae and Flaviviridae families; contacting said fraction, for a predetermined time interval in an appropriate culture medium, with permissive cells having an endocytosis path relayed by at least one receptor of the lipoproteins and modulated inter alia by an activating agent selected among an unsaturated fatty acid or an unsaturated fatty acid derivative comprising 16 to 20 carbon atoms or their mixture. The invention also concerns the uses of viral particles and polypeptides obtained by said method.

    Abstract translation: 本发明涉及一种培养，繁殖和复制本发明的披膜病毒科和黄病毒科的病毒的方法，其中包括：提供从血清或血浆中获得的至少一部分LVP 感染至少病毒的披头丝菌科和黄病毒科家族的病人; 在合适的培养基中使所述级分接触预定的时间间隔，其中容许细胞具有由至少一种脂蛋白受体中和的内吞途径，并特别通过选自不饱和脂肪酸或不饱和脂肪酸的活化剂调节 包含16-20个碳原子的衍生物或它们的混合物。 本发明还涉及通过所述方法获得的病毒颗粒和多肽的用途。

    微生物検出法及び微生物検出キット
    15.
    发明申请
    微生物検出法及び微生物検出キット 审中-公开
    微生物检测方法和微生物检测试剂盒

    公开(公告)号：WO2017010001A1

    公开(公告)日：2017-01-19

    申请号：PCT/JP2015/070410

    申请日：2015-07-16

    Applicant: 森永乳業株式会社

    Inventor： 副島　隆志

    IPC: C12Q1/02 ,  C12N15/00 ,  C12Q1/68

    CPC classification number: C12N15/00 ,  C12Q1/02 ,  C12Q1/68 ,  Y02A50/451 ,  Y02A50/60

    Abstract: 下記工程により、被検試料中の微生物の生細胞を、死細胞及び／又は損傷細胞と識別して検出する： ａ）前記被検試料に、微生物の核酸の核酸増幅法による増幅を、死細胞に選択的に阻害する薬剤を添加する工程、 ｂ）微生物の細胞の透過性を高める処理を行う工程、 ｃ）被検試料中の微生物の核酸のターゲット領域を、細胞からの核酸の抽出を行わずに、鎖置換型核酸伸長酵素を用いた等温核酸増幅法により増幅する工程、及び ｄ）増幅産物を解析する工程。

    Abstract translation: 根据本发明，使用以下步骤检测测试样品中微生物的活细胞，并将其与死细胞和/或受损细胞区分开：a）向测试样品中加入选择性抑制 死细胞，通过核酸扩增方法扩增微生物的核酸，b）进行加工以提高微生物细胞的通透性的步骤，c）通过 使用链置换核​​酸延伸酶的链段等温核酸扩增方法，不从细胞中提取核酸的测试样品中的微生物的核酸靶区，以及d）分析扩增产物的步骤。

    DNAZYME-NANOPARTICLE CONJUGATES AND METHODS OF USE THEREOF
    16.
    发明申请
    DNAZYME-NANOPARTICLE CONJUGATES AND METHODS OF USE THEREOF 审中-公开
    DNAZYME-NANOPARTICLE CONJUGATES及其使用方法

    公开(公告)号：WO2014201454A2

    公开(公告)日：2014-12-18

    申请号：PCT/US2014/042480

    申请日：2014-06-16

    Applicant: UNIVERSITY OF NOTRE DAME ,  CARTER, James R. ,  FRASER, Malcolm J.

    Inventor： CARTER, James R. ,  FRASER, Malcolm J.

    IPC: A61K39/12

    CPC classification number: C12N15/11 ,  C12N15/1131 ,  C12N2310/127 ,  C12N2310/351 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2770/24122 ,  C12Q1/6816 ,  C12Q1/701 ,  G01N33/5308 ,  G01N33/56983 ,  G01N33/588 ,  G01N2333/181 ,  G01N2333/185 ,  Y02A50/51 ,  Y02A50/53 ,  Y02A50/60 ,  C12Q2521/345 ,  C12Q2565/113

    Abstract: The present invention relates to DNAzymes (also known as deoxyribozymes, DNA enzymes, catalytic DNA, or DZ), which are conjugated to nanoparticles (NP) to facilitate the detection of nucleic acids. One aspect of the invention relates to compounds comprising DNAzymes conjugated to nanoparticles (DZ-NP), such as metallic or gold nanoparticles, and methods for their synthesis. Another aspect of the invention relates to methods of using the conjugated compounds to detect nucleic acids, such as genomic material or transcripts of infectious agents, such as viruses, exemplified by applications demonstrating visual detection of Flavivirus RNA molecules, such as dengue virus, or Alphavirus RNA molecules, such as chikungunya virus, in short time periods, using compositions comprising stable components.

    Abstract translation: 本发明涉及与纳米颗粒（NP）缀合以促进核酸检测的DNA酶（也称为脱氧核酶，DNA酶，催化DNA或DZ）。 本发明的一个方面涉及包含与纳米颗粒（DZ-NP）缀合的DNA酶的化合物，例如金属或金纳米颗粒，以及它们的合成方法。 本发明的另一方面涉及使用共轭化合物来检测核酸的方法，所述核酸例如基因组材料或感染因子的转录物，例如病毒，例如用于证实黄病毒RNA分子（例如登革热病毒）或甲病毒属（Alphavirus）的视觉检测 RNA分子，如基孔肯雅病毒，在短时间内使用包含稳定组分的组合物。

    MULTIPLEX IMMUNO SCREENING ASSAY
    17.
    发明申请
    MULTIPLEX IMMUNO SCREENING ASSAY 审中-公开
    多重免疫筛选测定

    公开(公告)号：WO2013164476A1

    公开(公告)日：2013-11-07

    申请号：PCT/EP2013/059312

    申请日：2013-05-03

    Applicant: INSTITUT PASTEUR

    Inventor： MANUGUERRA, Jean-Claude ,  VANHOMWEGEN, Jessica ,  DESPRES, Philippe ,  PAULOUS, Sylvie

    IPC: C12N9/10 ,  C12N15/62 ,  G01N33/543 ,  G01N33/564

    CPC classification number: G01N33/54306 ,  G01N33/54313 ,  G01N33/54353 ,  G01N33/564 ,  G01N33/56983 ,  G01N33/6845 ,  G01N2333/18 ,  G01N2333/185 ,  G01N2469/20 ,  Y02A50/51 ,  Y02A50/53 ,  Y02A50/56 ,  Y02A50/58 ,  Y02A50/60

    Abstract: The present invention provides kits and assay methods for the early detection of pathogens, precise identification of the etiologic agent, and improved disease surveillance. More specifically, the present invention discloses an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres), said support being previously coated with an AGT substrate. This coupling is mediated by the irreversible reaction of the AGT enzyme on its substrate. The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures. The methods of the invention possess the ability to multiplex, minimize the amount of biological sample, and have enhanced sensitivity and specificity toward target antibodies as compared with classical ELISA or Radio-Immunoprecipitation assays.

    Abstract translation: 本发明提供了用于病原体的早期检测，病原体的精确鉴定和改善的疾病监测的试剂盒和分析方法。 更具体地说，本发明公开了导致快速和同时检测抗感染病人的生物体液中的各种感染性病原体的抗体的免疫测定。 该免疫测定涉及包含AGT酶和病毒抗原的融合蛋白在可识别的固体载体（例如荧光微球）上的共价和定向偶联，所述载体预先用AGT底物包被。 该偶联由AGT酶在其底物上的不可逆反应介导。 与由标准胺偶联程序产生的抗原偶联微球体相比，由此获得的抗原偶联微球体显示出增强的特异性抗体捕获。 与传统的ELISA或放射性免疫沉淀测定相比，本发明的方法具有复合，最小化生物样品量的能力，并且对靶抗体具有增强的敏感性和特异性。

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