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    A METHOD FOR IDENTIFYING EFFECTOR MOLECULES FOR GENE NETWORK INTEGRATION
    21.
    发明申请
    A METHOD FOR IDENTIFYING EFFECTOR MOLECULES FOR GENE NETWORK INTEGRATION 审中-公开
    用于鉴定用于基因网络整合的效应分子的方法

    公开(公告)号：WO2003025229A1

    公开(公告)日：2003-03-27

    申请号：PCT/AU2002/001286

    申请日：2002-09-19

    Applicant: THE UNIVERSITY OF QUEENSLAND ,  MATTICK, John ,  GAGEN, Michael ,  STANLEY, Stefan

    Inventor： MATTICK, John ,  GAGEN, Michael ,  STANLEY, Stefan

    IPC: C12Q001/68

    CPC classification number: C12N15/1089 ,  C12N15/10 ,  C12N15/1034 ,  C12N15/63 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N33/5005 ,  G01N33/5308 ,  G01N2500/04 ,  G06F19/12 ,  G06F19/18 ,  G06F19/22

    Abstract: The present invention relates generally to the field of bioinformatics and its applications to functional genomics and advanced genetic engineering. More particularly, the present invention contemplates a method for identifying effector molecules capable of modulating gene network integration and which facilitate genetic multi-tasking and the regulation of complex suites of programmed responses within, on and between eukaryotic cells. The present invention permits, therefore, the identification of a new generation of proteome and nucleome modulators useful in a range of therapeutic and trait-modifying protocols. The ability to manipulate genetic networks within a cell and within whole organisms also provides a sophisticated genetic engineering approach of introducing new traits and to influencing the genetic architecture and, hence, to enable cell and organismal programming or re-programming. The identification of effector molecules and their target or receiver sites, further enables the development of diagnostic protocols for a range of conditions or physiological or genetic states of an organism useful, for example, in modulating stem cell differentiation, quantitative traits, aging or the development of pathological conditions.

    Abstract translation: 本发明一般涉及生物信息学领域及其在功能基因组学和先进基因工程中的应用。 更具体地，本发明考虑了一种用于鉴定能够调节基因网络整合的效应分子的方法，并且促进遗传多任务和调控真核细胞内，真核细胞之间和之间的编程应答的复杂套件。 因此，本发明允许鉴定在一系列治疗和性状修饰方案中有用的新一代蛋白质组和核糖调节剂。 在细胞内和整个生物体内操纵遗传网络的能力还提供了一种复杂的遗传工程方法，引入新的特征和影响遗传结构，从而实现细胞和生物编程或重新编程。 识别效应分子及其靶点或接收部位进一步使得可以开发用于一系列生物或生物或遗传状态的诊断方案，所述生物或生物或遗传状态可用于例如调节干细胞分化，数量性状，衰老或发育 的病理状况。

    AUTOMATED METHODS OF DETECTING RECEPTOR ACTIVITY
    25.
    发明申请
    AUTOMATED METHODS OF DETECTING RECEPTOR ACTIVITY 审中-公开
    自动检测受体活性的方法

    公开(公告)号：WO2002073199A1

    公开(公告)日：2002-09-19

    申请号：PCT/US2002/007434

    申请日：2002-03-13

    Applicant: DUKE UNIVERSITY ,  BARAK, Larry, S. ,  OAKLEY, Robert, H.

    Inventor： BARAK, Larry, S. ,  OAKLEY, Robert, H.

    IPC: G01N33/53

    CPC classification number: C12N15/1089 ,  C12N2503/00 ,  G01N33/5076 ,  G01N33/566 ,  G01N2333/726 ,  G01N2500/04 ,  G01N2500/10

    Abstract: Methods of detecting G protein-coupled receptor (GPCR) activity in vitro and in vivo are provided. In one embodiment, the method includes providing at least one cell that expresses a GPCR and a plurality of conjugated proteins. Each of the plurality of conjugated proteins is formed by conjugating an arrestin protein and a detectable molecule. The plurality of conjugated proteins are substantially evenly distributed in the cytoplasm of the at least one cell. A first image of the at least one cell is obtained by detecting an amount of energy emitted from the detectable molecules and storing a value relative to the amount of energy. The at least one cell is treated with an agonist. A second image of the at least one cell is obtained. The first image and the second image are compared to detect the localization of at least some of the plurality of conjugated proteins at endocytic vesicles and/or endosomes.

    Abstract translation: 提供了在体外和/或体内检测G蛋白偶联受体（GPCR）活性的方法。 在一个实施方案中，该方法包括提供至少一个表达GPCR和多个缀合蛋白的细胞。 通过将抑制蛋白和可检测分子结合来形成多个共轭蛋白质中的每一个。 多个缀合的蛋白质基本上均匀地分布在至少一个细胞的细胞质中。 通过检测从可检测分子发射的能量的量并且存储相对于能量的值来获得至少一个细胞的第一图像。 用激动剂治疗至少一个细胞。 获得至少一个单元的第二图像。 比较第一图像和第二图像以检测多个缀合蛋白质中的至少一些在胞吞小泡和/或内体上的定位。

    SYNTHETIC GENES FOR ENHANCED EXPRESSION
    26.
    发明申请
    SYNTHETIC GENES FOR ENHANCED EXPRESSION 审中-公开
    合成基因增加表达

    公开(公告)号：WO01055342A3

    公开(公告)日：2002-01-17

    申请号：PCT/US2001/003186

    申请日：2001-01-31

    IPC: C07K14/21 ,  C12N15/00 ,  C07H21/02 ,  G01N31/00

    CPC classification number: C07K14/21 ,  C12N15/1089 ,  C12N15/67

    Abstract: A synthetic nucleic acid sequence is disclosed, comprising a non-naturally occurring polymer of nucleic acids, having a biological function encoded by the sequence and known from a starting nucleic acid sequence, and having a difference in sequence of at least about 5 % between the nucleic acids of the synthetic sequence and the starting sequence. The difference between the nucleic acid sequences results in a different free energy of folding for the synthetic sequence as compared to the starting sequence, such that the synthetic sequence would be expressed better in a selected heterologous host cell than the starting sequence would be if expressed in the same heterologous host cell.

    Abstract translation: 本发明涉及含有核酸的非天然聚合物的合成核酸序列，其具有由该序列编码并且从起始核酸序列已知的生物学功能，并且在 在合成序列的核酸和起始序列之间至少约5％的序列。 与起始序列相比，核酸序列之间的差异导致合成序列的不可重折叠自由能不同，使得合成序列在选择的异源宿主细胞中比在 如果它在相同的异源宿主细胞中表达，则不会。

    METHOD OF SCREENING CANDIDATE FOR ARTIFICIAL PROMOTER
    28.
    发明申请
    METHOD OF SCREENING CANDIDATE FOR ARTIFICIAL PROMOTER 审中-公开
    筛选用于人造促进剂的候选药物的方法

    公开(公告)号：WO01027259A1

    公开(公告)日：2001-04-19

    申请号：PCT/JP2000/007105

    申请日：2000-10-12

    IPC: C12N15/10 ,  C12N15/09

    CPC classification number: C12N15/1089

    Abstract: A method of screening a candidate for an artificial promoter whereby the number of promoters to be subjected to an experiment in practice can be reduced in designing a candidate for an artificial promoter effective on a structural gene. This method comprises: (S1) ligating an arbitrarily designed base sequence to the upstream of a structural gene; (S2, S3) extracting bases from a definite domain containing at least the vicinity of the transcriptional initiation point of the base sequence thus formed by the binding in a definite pattern and determining an index curve from the imaginary amino acid sequence thus formed; and (S4), when the index curve thus determined shows a reversal from positive to negative in the vicinity of the transcriptional initiation point, then selecting the base sequence ligated to the upstream of the structural gene as a candidate for an artificial promoter. By using this method, base sequences with a high possibility of acting as an effective promoter can be screened prior to an experiment. Thus, the number of promoters to be subjected to the experiment in practice can be reduced.

    Abstract translation: 筛选候选人人工促进剂的方法，其中在设计对结构基因有效的人工启动子的候选物时可以减少在实践中经受实验的启动子的数量。 该方法包括：（S1）将任意设计的碱基序列连接到结构基因的上游; （S2，S3）从确定的结构域提取碱基，该定义结构域至少包含通过以确定的图案结合形成的碱基序列的转录起始点附近，并从由此形成的假想氨基酸序列确定指数曲线; 和（S4），当由此确定的指标曲线显示在转录起始点附近从正转为负时，则选择连接到结构基因上游的碱基序列作为人工启动子的候选物。 通过使用该方法，可以在实验前筛选具有高可能性作为有效启动子的碱基序列。 因此，可以减少在实践中进行实验的启动子的数量。

    A SYSTEM FOR CELL-BASED SCREENING
    30.
    发明申请
    A SYSTEM FOR CELL-BASED SCREENING 审中-公开
    基于小区筛选的系统

    公开(公告)号：WO0070342A2

    公开(公告)日：2000-11-23

    申请号：PCT/US0013208

    申请日：2000-05-12

    Applicant: CELLOMICS INC ,  OLSON KEITH ,  LAPETS OLEG

    Inventor： OLSON KEITH ,  LAPETS OLEG

    IPC: C12N15/10 ,  G01N15/14 ,  G01N33/50

    CPC classification number: C12N15/1089 ,  G01N15/1463 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5035 ,  G01N33/5044 ,  G01N33/5067 ,  G01N33/5076

    Abstract: The present invention provides systems, methods, screens, and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of luminescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing luminescent reporter molecules in an array of locations and scanning numerous cells in each location with a luminescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the luminescently labeled reporter molecules in the cells.

    Abstract translation: 本发明提供用于细胞光学系统分析的系统，方法，筛选和试剂盒，以快速确定细胞中发光标记的报告分子的分布，环境或活性，目的是筛选特定影响特定物质的化合物的大量化合物 生物功能。 本发明涉及在位置阵列中提供含有发光报道分子的细胞，并用发光光学系统扫描每个位置中的多个细胞，将光学信息转换为数字数据，并利用数字数据来确定其分布，环境或活动 荧光标记的细胞中的报道分子。

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