公开(公告)号：WO2005080566A1

公开(公告)日：2005-09-01

申请号：PCT/US2005/004593

申请日：2005-02-14

Applicant: INTEL CORPORATION ,  SU, Xing ,  SUNDARARAJAN, Narayanan ,  KOO, Tae-Woong

Inventor： SU, Xing ,  SUNDARARAJAN, Narayanan ,  KOO, Tae-Woong

IPC: C12N15/10

CPC classification number: G01N33/54353 ,  B01L3/5027 ,  C12N15/1006 ,  C12Q1/6869 ,  C12Q2565/629 ,  C12Q2521/319 ,  C12Q2565/518

Abstract: Devices and methods for isolating, detecting, and positioning single polymeric molecules without the need for expensive equipment are provided. The disclosed devices and methods allow for a molecule to be quickly and efficiently transported to a specific sub-micron area. Such devices are useful, for instance, for performing analyses in which the sequence of a polymer of interest is determined.

Abstract translation: 提供了用于隔离，检测和定位单个聚合物分子的装置和方法，而不需要昂贵的设备。 所公开的装置和方法允许将分子快速有效地运送到特定的亚微米区域。 这样的装置例如用于进行其中确定感兴趣聚合物序列的分析是有用的。

公开(公告)号：WO2005066369A2

公开(公告)日：2005-07-21

申请号：PCT/US2004/043633

申请日：2004-12-28

Applicant: INTEL CORPORATION ,  BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan

Inventor： BERLIN, Andrew ,  SU, Xing ,  CHAN, Selena ,  KOO, Tae-Woong ,  SUN, Lei ,  SUNDARARAJAN, Narayan

IPC: C12Q1/68

CPC classification number: G01N21/658 ,  C12Q1/6869 ,  C12Q2565/632 ,  C12Q2533/101

Abstract: The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.

Abstract translation: 本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。 该方法涉及使用表面增强拉曼光谱（SERS）或表面增强相干抗斯托克斯拉曼光谱（SECARS）检测核苷酸，核苷或碱基。 检测可以是在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取的核酸测序反应的一部分。 合成的新生链的核酸序列和模板链的互补序列可以通过跟踪聚合反应期间核苷酸掺入的顺序来确定。 提供了通过从糖部分切割碱来增强核苷酸或核苷的SERS信号的方法。 此外，提供了用于检测单碱基重复的方法。

公开(公告)号：WO2005066367A2

公开(公告)日：2005-07-21

申请号：PCT/US2004/043364

申请日：2004-12-24

Applicant: INTEL CORPORATION ,  SU, Xing ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo ,  BERLIN, Andrew ,  SUN, Lei ,  ZHANG, Yuegang

Inventor： SU, Xing ,  SUNDARARAJAN, Narayan ,  YAMAKAWA, Mineo ,  BERLIN, Andrew ,  SUN, Lei ,  ZHANG, Yuegang

IPC: C12Q1/68

CPC classification number: B82Y40/00 ,  B82Y10/00 ,  B82Y30/00 ,  C01B32/162 ,  C01B2202/08 ,  C01B2202/36 ,  D01F9/127 ,  H01L51/0048 ,  H01L51/0052

Abstract: The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles (140, 230) to polymer (120, 210) molecules, attaching the polymer (120, 210) molecules to a substrate, removing the polymer (120, 210) molecules and producing carbon nanotubes on the catalyst nanoparticles (140, 230). The polymer (120, 210) molecules alignment techniques. The nanotube arrays can be attached to selected areas (110, 310) of the substrate. Within the selected areas (110, 310), the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

Abstract translation: 本文公开的方法，装置和系统涉及碳纳米管的有序阵列。 在本发明的具体实施方案中，通过包括将催化剂纳米颗粒（140,230）附着到聚合物（120,210）分子上的方法形成纳米管阵列，将聚合物（120,210）分子连接到基底上，去除聚合物 120,210）分子并在催化剂纳米颗粒（140,230）上产生碳纳米管。 聚合物（120,210）分子对齐技术。 纳米管阵列可以附着到基板的选定区域（110,310）。 在所选择的区域（110,310）内，纳米管是非随机分布的。 本文公开的其它实施方案涉及包括连接到衬底的纳米管的有序阵列的装置以及包括通过所要求保护的方法产生的连接到衬底的碳纳米管的有序阵列的系统。 在某些实施方案中，本文提供了通过将分子线连接到双链DNA分子来对齐分子线的方法。

公开(公告)号：WO2005065541A2

公开(公告)日：2005-07-21

申请号：PCT/US2004043769

申请日：2004-12-29

Applicant: INTEL CORP ,  BERLIN ANDREW ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  SUN LEI ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

Inventor： BERLIN ANDREW ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  SUN LEI ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

IPC: G01N21/65 ,  G01N33/58 ,  G01N33/68 ,  A61B5/00

CPC classification number: G01N21/658 ,  G01N33/6803 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

Abstract: The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

Abstract translation: 本发明提供了用于分析生物样品的蛋白质含量的方法，例如获得由特定个体提供的样品的蛋白质谱。 样品中的蛋白质和蛋白质片段基于化学和/或物理性质分离，并在固体基质上或流动液体流中的离散位置处保持分离状态。 然后检测拉曼光谱，由离散位置处的分离的蛋白质或片段产生，使得离散位置的光谱提供关于在离散位置处的一种或多种特定蛋白质或片段的结构或身份的信息。 离散位置处的蛋白质或片段可以用金属（例如金或银）涂覆，和/或分离的蛋白质可与化学增强剂接触以提供SERS光谱。 还提供了用于实施本发明的方法和试剂盒。