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    MODULATION OF ACCESSIBILITY OF HOST NUCLEIC ACIDS TO NUCLEIC ACID DIGESTING ENZYMES IN ACELLULAR BIOLOGICAL FLUIDS
    1.
    发明申请
    MODULATION OF ACCESSIBILITY OF HOST NUCLEIC ACIDS TO NUCLEIC ACID DIGESTING ENZYMES IN ACELLULAR BIOLOGICAL FLUIDS 审中-公开
    主链核酸对细胞生物流体中核酸消化酶的调控

    公开(公告)号：WO2017118718A1

    公开(公告)日：2017-07-13

    申请号：PCT/EP2017/050250

    申请日：2017-01-06

    Applicant: PATHOQUEST

    Inventor： CABANNES, Éric

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1003 ,  C12Q2521/301 ,  C12Q2521/319 ,  C12Q2521/327

    Abstract: The present invention relates to a method for enriching an infectious agent in an acellular fraction of a biological fluid using detergents and nucleic acids-digesting enzymes. The present invention also relates to a method for modulating the accessibility of host nucleic acids (HNA) in an acellular fraction of a biological fluid using detergents and nucleic acids-digesting enzymes, and to a method for detecting infectious agents in an acellular fraction from a biological fluid.

    Abstract translation: 本发明涉及使用去污剂和核酸消化酶在生物流体的无细胞部分中富集感染因子的方法。 本发明还涉及使用去污剂和核酸消化酶来调节生物流体的无细胞部分中的宿主核酸（HNA）的可接近性的方法，并且涉及用于检测来自 生物流体。

    METHOD FOR IDENTIFICATION AND ENUMERATION OF NUCLEIC ACID SEQUENCE, EXPRESSION, COPY, OR DNA METHYLATION CHANGES, USING COMBINED NUCLEASE, LIGASE, POLYMERASE, AND SEQUENCING REACTIONS
    7.
    发明申请
    METHOD FOR IDENTIFICATION AND ENUMERATION OF NUCLEIC ACID SEQUENCE, EXPRESSION, COPY, OR DNA METHYLATION CHANGES, USING COMBINED NUCLEASE, LIGASE, POLYMERASE, AND SEQUENCING REACTIONS 审中-公开
    使用组合核酸，胶原，聚合酶和序列反应的核酸序列，表达，复制或DNA甲基化变化的鉴定和产生的方法

    公开(公告)号：WO2015188192A3

    公开(公告)日：2016-05-26

    申请号：PCT/US2015034724

    申请日：2015-06-08

    Applicant: UNIV CORNELL

    Inventor： BARANY FRANCIS ,  EFCAVITCH JOHN WILLIAM

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2531/125 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2565/501 ,  C12Q2521/319 ,  C12Q2521/325 ,  C12Q2521/331 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2525/191 ,  C12Q2525/307 ,  C12Q2563/179

    Abstract: The present invention relates to a method for the highly specific, targeted capture of regions of human genomes and transcriptomes from the blood, i.e. from cell free circulating DNA, exosomes, microRNA, circulating tumor cells, or total blood cells, to allow for the highly sensitive detection of mutation, expression, copy number, translocation, alternative splicing, and methylation changes using combined nuclease, ligation, polymerase, and massively parallel sequencing reactions. The method generates a collection of different circular chimeric single- stranded nucleic acid constructs, suitable for sequencing on multiple platforms. In some embodiments, each construct of the collection comprised a first single stranded segment of original genomic DNA from a host organism and a second single stranded synthetic nucleic acid segment that is linked to the first single stranded segment and comprises a nucleotide sequence that is exogenous to the host organism. These chimeric constructs are suitable for identifying and enumerating mutations, copy changes, translocations, and methylation changes. In other embodiments, input mRNA, IncRNA, or miRNA is used to generate circular DNA products that reflect the presence and copy number of specific mRNA's, IncRNA's splice-site variants, translocations, and miRNA.

    Abstract translation: 本发明涉及一种用于高度特异性靶向捕获来自血液的人类基因组和转录组的区域的方法，即来自无细胞循环的DNA，外来体，微小RNA，循环肿瘤细胞或全血细胞，以允许高度的 使用组合核酸酶，连接，聚合酶和大规模平行测序反应敏感检测突变，表达，拷贝数，易位，选择性剪接和甲基化变化。 该方法产生适合于在多个平台上进行测序的不同圆形嵌合单链核酸构建体的集合。 在一些实施方案中，收集物的每个构建体包含来自宿主生物体的原始基因组DNA的第一单链区段和连接到第一单链区段的第二单链合成核酸区段，并且包含外源至 宿主生物体。 这些嵌合构建体适用于鉴定和列举突变，拷贝变化，易位和甲基化变化。 在其他实施方案中，使用输入mRNA，IncRNA或miRNA来产生环状DNA产物，其反映特异性mRNA，IncRNA的剪接位点变体，易位和miRNA的存在和拷贝数。

    NUCLEIC ACID ANALYSIS
    8.
    发明申请
    NUCLEIC ACID ANALYSIS 审中-公开
    核酸分析

    公开(公告)号：WO2016034892A1

    公开(公告)日：2016-03-10

    申请号：PCT/GB2015/052561

    申请日：2015-09-04

    Applicant: MOORLODGE BIOTECH VENTURES LIMITED

    Inventor： MINTER, Stephen John ,  PATSOS, Georgios

    IPC: C12Q1/68

    CPC classification number: C12Q1/6823 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6897 ,  C12Q2521/319 ,  C12Q2537/155 ,  C12Q2563/107 ,  C12Q2565/1015

    Abstract: A method of analysing for a single stranded nucleic acid present, or potentially present, in a sample comprises a step in which the target nucleic acid (if present in the sample) displaces – and hybridises to – a reporter strand originally present in an interrogating duplex structure comprised of the reporter strand and a displaceable shorter strand. The reporter strand is tagged at or adjacent one end thereof with a reporter moiety capable of providing a detectable signal. The reporter/target duplex structure is such that the reporter strand may be selectively enzymatically digested (e.g. by means of λ- exonuclease) from its end opposite the reporter moiety to release that moiety for direct or indirect detection and regenerate single stranded target, which may then cycle through a plurality of the displacement and digestion steps to result in amplification of signal.

    Abstract translation: 分析样品中存在或潜在存在的单链核酸的方法包括其中靶核酸（如果存在于样品中）置换并与最初存在于询问双联体中的报道分子杂交的步骤 结构由报道链和可置换的短链组成。 报告物链在其一端或相邻的一端标记能够提供可检测信号的报告分子。 报告基因/靶双链体结构可以使得报道链可以从其与报道部分相对的端部选择性酶消化（例如通过λ-核酸外切酶）释放该部分用于直接或间接检测并再生单链靶标，其可以 然后循环通过多个位移和消化步骤以导致信号的放大。

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