公开(公告)号：WO2009030217A2

公开(公告)日：2009-03-12

申请号：PCT/DE2008/001470

申请日：2008-09-03

Applicant: MEDICYTE GMBH ,  BRASPENNING, Adrianus, J., C., M. ,  HOLDER, Stefan ,  KÜPPER, Heiner

Inventor： BRASPENNING, Adrianus, J., C., M. ,  HOLDER, Stefan ,  KÜPPER, Heiner

IPC: C12N5/00

CPC classification number: C12N5/00 ,  C12N2501/40

Abstract: Die Erfindung betrifft ein Verfahren zur Vermehrung oder Anreicherung von primären Zellen ohne Tumoreigenschaften und deren weitere Verwendung.

Abstract translation: 本发明涉及一种用于繁殖或积累没有肿瘤特性的原代细胞的方法及其进一步的用途。

公开(公告)号：WO2008137115A1

公开(公告)日：2008-11-13

申请号：PCT/US2008/005742

申请日：2008-05-05

Applicant: THE BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  CRAWFORD, Keith, W.

Inventor： CRAWFORD, Keith, W.

IPC: C12N5/08 ,  C12N5/22 ,  C12Q1/68

CPC classification number: A61K35/24 ,  A61K35/12 ,  A61K35/14 ,  C12N5/0639 ,  C12N5/0647 ,  C12N5/0653 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0657 ,  C12N5/0658 ,  C12N5/0661 ,  C12N5/0662 ,  C12N5/0668 ,  C12N5/067 ,  C12N5/0676 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/125 ,  C12N2501/135 ,  C12N2501/155 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/26 ,  C12N2501/39 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/999

Abstract: The invention provides a quiescent stem cell having the capacity to differentiate into ectoderm, mesoderm and endoderm, and which does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105 and CD90. The invention further provides a proliferative stem cell, which expresses genes including Oct-4, Nanog, Sox2, GDF3, P16INK4, BMI, Notch, HDAC4, TERT, Rex-1 and TWIST but does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105 and CD90. The cells of the invention can be isolated from adult mammals, have embryonic cell characteristics, and can form embryoid bodies. Methods for obtaining the stem cells, as well as methods of treating diseases and differentiated the stem cells, are also provided.

Abstract translation: 本发明提供了具有分化成外胚层，中胚层和内胚层的能力的静止干细胞，其不表达包括MHC I类，MHC II类，CD44，CD45，CD13，CD34，CD49c，CD73，CD105和 CD90。 本发明还提供了一种增殖干细胞，其表达包括Oct-4，Nanog，Sox2，GDF3，P16INK4，BMI，Notch，HDAC4，TERT，Rex-1和TWIST的基因，但不表达包括MHC I类， MHC II类，CD44，CD45，CD13，CD34，CD49c，CD73，CD105和CD90。 本发明的细胞可以与成年哺乳动物分离，具有胚胎细胞特征，并且可以形成胚状体。 还提供了获得干细胞的方法，以及治疗疾病和分化干细胞的方法。

公开(公告)号：WO2008120201A2

公开(公告)日：2008-10-09

申请号：PCT/IL2008/000435

申请日：2008-03-27

Applicant: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LTD. ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE ,  ABERDAM, Daniel ,  ABERDAM, Edith ,  BARAK, Efrat ,  AMIT, Michal ,  ITSKOVITZ-ELDOR, Joseph

Inventor： ABERDAM, Daniel ,  ABERDAM, Edith ,  BARAK, Efrat ,  AMIT, Michal ,  ITSKOVITZ-ELDOR, Joseph

IPC: C12N5/08

CPC classification number: C12N5/0603 ,  C12N2501/155 ,  C12N2501/40 ,  C12N2501/60 ,  C12N2502/1394 ,  C12N2506/02 ,  C12N2510/00

Abstract: An isolated homogeneous population of cells comprising a plurality of human ectodermal progenitor cells. Also provided are methods of generating and using the population of cells.

Abstract translation: 分离的包含多个人类外胚层祖细胞的均质细胞群。 还提供了产生和使用细胞群体的方法。

公开(公告)号：WO2008118957A2

公开(公告)日：2008-10-02

申请号：PCT/US2008/058261

申请日：2008-03-26

Applicant: THE GOVERNMENT OF U.S.A AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH & HUMAN SERVICES ,  KO, Minoru, S.h. ,  FALCO, Geppino ,  LEE, Sung-lim ,  MONTI, Manuela ,  STANGHELLINI, Ilaria

Inventor： KO, Minoru, S.h. ,  FALCO, Geppino ,  LEE, Sung-lim ,  MONTI, Manuela ,  STANGHELLINI, Ilaria

IPC: C12N5/00 ,  C12N15/00

CPC classification number: C07K14/47 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/0393 ,  C12N5/0606 ,  C12N5/0611 ,  C12N15/113 ,  C12N15/85 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2501/40 ,  C12N2510/00 ,  C12N2830/008 ,  C12N2830/85

Abstract: Described herein is Zscan4, a gene exhibiting 2-cell embryonic stage and embryonic stem cell specific expression. Identification of nine Zscan4 co-expressed genes is also described. Inhibition of Zscan4 expression inhibits the 2-cell to 4-cell embryonic transition and prevents blastocyst implantation, expansion and outgrowth. Provided herein are methods of inhibiting differentiation of a stem cell, promoting blastocyst outgrowth of embryonic stem cells and identifying a subpopulation of stem cells expressing Zscan4. Further described is the identification of Trim43 as a gene exhibiting morula-specific expression. Also provided are isolated expression vectors comprising a Zscan4 promoter, or a Trim43 promoter operably linked to a heterologous polypeptide and uses thereof. Further provided are transgenic animals comprising transgenes encoding marker proteins operably linked to Zscan4 and Trim43 promoters.

Abstract translation: 本文描述的是Zscan4，其表现出2-细胞胚胎期和胚胎干细胞特异性表达的基因。 还描述了9种Zscan4共表达基因的鉴定。 抑制Zscan4表达抑制2细胞到4细胞胚胎转换，并防止囊胚植入，扩张和生长。 本文提供了抑制干细胞分化，促进胚胎干细胞的胚泡生长和鉴定表达Zscan4的干细胞亚群的方法。 进一步描述了鉴定Trim43作为表达桑ula特异性表达的基因。 还提供了分离的表达载体，其包含与异源多肽可操作地连接的Zscan4启动子或Trim43启动子及其用途。 还提供了包含编码与Zscan4和Trim43启动子可操作地连接的标记蛋白的转基因的转基因动物。

公开(公告)号：WO2007080919A1

公开(公告)日：2007-07-19

申请号：PCT/JP2007/050232

申请日：2007-01-11

Applicant: 独立行政法人科学技術振興機構 ,  株式会社ツーセル ,  加藤 幸夫 ,  邵 金昌 ,  桂 由紀 ,  辻 紘一郎

Inventor： 加藤 幸夫 ,  邵 金昌 ,  桂 由紀 ,  辻 紘一郎

IPC: C12N5/06

CPC classification number: C12N5/0663 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/40

Abstract: 　特定の増殖因子及び少なくとも１つのリン脂質を含有する培地、並びに当該培地を作製するための組成物、キット及び方法を提供する。これにより、無血清培地又は低血清培地でありながら血清含有培地に匹敵する動物細胞増殖促進効果を有する技術を実現する。

Abstract translation: 公开了：含有特定生长因子和至少一种磷脂的培养基; 用于制备培养基的组合物; 一套工具 和一种方法。 可以提供一种技术，其使用无血清或低血清培养基，并且对与血清培养基中的培养获得的促进作用相当的动物细胞增殖具有促进作用。

公开(公告)号：WO2007002385A3

公开(公告)日：2007-07-19

申请号：PCT/US2006024462

申请日：2006-06-23

Applicant: SINAI SCHOOL MEDICINE ,  KELLER GORDON M ,  GADUE PAUL

Inventor： KELLER GORDON M ,  GADUE PAUL

IPC: A01K67/00 ,  A01N1/02 ,  C12N5/071 ,  C12N5/0735

CPC classification number: C12N5/067 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/0607 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/03

Abstract: The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy. The present invention further provides a method of generating hepatocytes, cell populations enriched for hepatocytes, and a method of hepatocyte replacement therapy.

Abstract translation: 本发明提供富集中内胚层和中胚层的细胞群以及富含内胚层的细胞群。 本发明的细胞群可用于产生用于细胞替代疗法的细胞。 本发明还提供了产生肝细胞的方法，富含肝细胞的细胞群，以及肝细胞替代疗法。

公开(公告)号：WO2007016485A2

公开(公告)日：2007-02-08

申请号：PCT/US2006029736

申请日：2006-07-31

Applicant: ATHERSYS INC ,  MAYS ROBERT W

Inventor： MAYS ROBERT W

IPC: C12N5/02 ,  A61K35/12 ,  C12N5/074

CPC classification number: C12N5/0607 ,  C12N2501/40 ,  C12N2501/415

Abstract: The present invention is directed to the culture of non-embryonic cells, that can differentiate into cell types of more than one embryonic lineage, in culture under conditions that maintain differentiation capacity during expansion; more particularly, culturing non-embryonic cells in the presence of at least one GKS-3 inhibitor, such as BIO.

Abstract translation: 本发明涉及在扩展期间维持分化能力的条件下培养的非胚胎细胞的培养物，其可以分化成多于一种胚胎谱系的细胞类型; 更具体地，在存在至少一种GKS-3抑制剂如BIO的情况下培养非胚胎细胞。

公开(公告)号：WO2006027545A2

公开(公告)日：2006-03-16

申请号：PCT/GB2005/003206

申请日：2005-08-17

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH ,  LIM, Sai, Kiang ,  QUE, Jian, Wen ,  EL OAKLEY, Reida, Menshawe

Inventor： LIM, Sai, Kiang ,  QUE, Jian, Wen ,  EL OAKLEY, Reida, Menshawe

IPC: C12N5/06 ,  A61K38/45 ,  A61K31/436 ,  A61K31/045 ,  A61K31/661 ,  G01N33/50 ,  A01K67/027

CPC classification number: C12N5/0611 ,  A01K2217/075 ,  A01K2227/105 ,  A61K31/045 ,  A61K31/436 ,  A61K31/661 ,  A61K38/45 ,  C12N5/0604 ,  C12N5/0606 ,  C12N2501/04 ,  C12N2501/06 ,  C12N2501/40 ,  C12N2501/70

Abstract: We provide a method comprising modulating the activity of mTOR in a stem cell, preferably increasing the activity of mTOR to enable a stem cell to self-renew, or decreasing it to enable a stem cell to differentiate. The stem cell may be exposed to an agonist of mTOR for the former, preferably phosphatidic acid, or an antagonist of mTOR for the latter, preferably 1-butanol or rapamycin or a derivative thereof.

Abstract translation: 我们提供了一种方法，其包括调节干细胞中mTOR的活性，优选增加mTOR的活性以使干细胞自我更新或降低干细胞以使其能够分化。 干细胞可暴露于前者的mTOR激动剂，优选磷脂酸，或后者的mTOR拮抗剂，优选1-丁醇或雷帕霉素或其衍生物。

公开(公告)号：WO2004048408A8

公开(公告)日：2005-08-04

申请号：PCT/NL0300831

申请日：2003-11-25

Applicant: AZ UNIV AMSTERDAM ,  PEPPELENBOSCH MAIKEL PETRUS ,  ZIVKOVIC DANICA ,  DIKS SANDER ,  BINK ROBERT JOZEF

Inventor： PEPPELENBOSCH MAIKEL PETRUS ,  ZIVKOVIC DANICA ,  DIKS SANDER ,  BINK ROBERT JOZEF

IPC: C07K14/47 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/0797 ,  C12N5/06 ,  C07K14/435 ,  C07K14/46

CPC classification number: C12N5/0623 ,  C07K14/47 ,  C12N2501/40 ,  C12N2501/41

Abstract: The present invention relates to methods for expansion of stem or progenitor cells. These methods rely on Asb-a polypeptides or nucleic acids to temporarily suppress differentiation of the cells, thus allowing proliferation and self-renewal of the stem or progenitor cells. Abs-a polypeptides and coding sequences define a class of polypeptides and nucleic acids that are both structurally and functionally highly conserved among vertebrates. Asb-a polypeptides contain 6 ankyrin repeats and a SOCS box that mediate the effect of the polypeptide on the regulation of specific subsets of genes involved in differentiation. The invention discloses various methods to increase the intracellular concentration of an Asb-a polypeptide for suppression of terminal differentiation of the stem or progenitor cells. The invention further relates to Asb-a polypeptides and nucleic acids, vectors and host cells for use in methods for their production and for use in the method for expansion of stem or progenitor cells, as well as to stem or progenitor cells containing exogenous Asb-a polypeptides and nucleic acids.

Abstract translation: 本发明涉及用于扩增干细胞或祖细胞的方法。 这些方法依赖于Asb-a多肽或核酸来暂时抑制细胞的分化，从而允许干细胞或祖细胞的增殖和自我更新。 Abs-多肽和编码序列限定了在脊椎动物中在结构和功能上高度保守的一类多肽和核酸。 Asb-a多肽含有6个锚蛋白重复序列​​和SOCS盒，其介导多肽对参与分化的基因的特定亚群的调节的影响。 本发明公开了增加用于抑制干细胞或祖细胞末端分化的Asb-a多肽的细胞内浓度的各种方法。 本发明进一步涉及Asb-a多肽和核酸，载体和宿主细胞，用于其生产方法和用于扩增干细胞或祖细胞的方法，以及含有外源Asb- 多肽和核酸。

公开(公告)号：WO2003093487A1

公开(公告)日：2003-11-13

申请号：PCT/AU2003/000518

申请日：2003-05-02

Applicant: THE VICTOR CHANG CARDIAC RESEARCH INSTITUTE LIMITED ,  HARVEY, Richard, Hans ,  YEOH, Thomas

Inventor： HARVEY, Richard, Hans ,  YEOH, Thomas

IPC: C12N005/02

CPC classification number: C12N5/0659 ,  C12N2501/40 ,  C12N2501/42 ,  C12N2503/02

Abstract: The invention relates to methods for controlling precursor cell differentiation by treating a precursor cell with an agent that affects activity of mitogen-activated protein kinase (MAPK) p38 or signalling of Notch in the cell such that the cell undergoes differentiation or undergoes self renewal, and cell models for studying cell differentiation and self renewal.

Abstract translation: 本发明涉及通过用影响丝裂原活化蛋白激酶（MAPK）p38的活性或Notch在细胞中的信号传导的药剂来处理前体细胞来控制前体细胞分化的方法，使细胞进行分化或进行自我更新，以及 用于研究细胞分化和自我更新的细胞模型。