公开(公告)号：WO2019143807A1

公开(公告)日：2019-07-25

申请号：PCT/US2019/013997

申请日：2019-01-17

Applicant: THE SECANT GROUP, LLC

Inventor： GABRIELE, Peter D. ,  HARRIS, Jeremy J. ,  NICHOLSON, Charles Brendan ,  LU, Steven ,  ROBERTSON, Jeffrey H. ,  PERON, Gael

IPC: C08J7/12 ,  C08K3/26 ,  C08K3/32 ,  C08G63/685 ,  C08K5/17 ,  A61L27/44 ,  C08G63/91 ,  C08L67/00 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12N5/0068 ,  C12M23/14 ,  C12M23/20 ,  C12M23/26 ,  C12M23/28 ,  C12M23/52 ,  C12M25/14 ,  C12M41/46 ,  C12N2533/30

Abstract: A biocontainment vessel includes a vessel structure including a structural composition and an enhancement composition associated with the structural composition. The enhancement composition includes a co-polymer. The co-polymer is a poly(glycerol sebacate) or a poly(glycerol sebacate urethane). The enhancement composition may also include an augmentation agent associated with the co-polymer. The enhancement composition is located with respect to the structural composition such that the enhancement composition benefits biological cells contained in the biocontainment vessel. A composition includes a co-polymer and an augmentation agent contained by the co-polymer. A method of containing biological cells includes placing the biological cells in an augmented biocontainment vessel and storing them in the augmented biocontainment vessel under predetermined conditions. An augmented substrate includes a substrate and an enhancement composition coating a surface of the substrate.

公开(公告)号：WO2018134359A1

公开(公告)日：2018-07-26

申请号：PCT/EP2018/051324

申请日：2018-01-19

Applicant: NATIONAL UNIVERSITY OF SINGAPORE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Inventor： VIASNOFF, Virgile ,  ARASI, Bakya

IPC: C12M1/00 ,  B01L3/00

CPC classification number: C12M23/12 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/044 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/163 ,  C12M23/20 ,  C12M47/00 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/149

Abstract: Plaque pour la biologie cellulaire, comprenant un support (1) biocompatible, un empilement sur le support (1) de films souples biocompatibles, l'empilement comprenant un puits traversant l'empilement jusqu'au support, dans laquelle les films de l'empilement sont engagés, entre eux et avec le support, dans une liaison mécanique permettant le pelage.

公开(公告)号：WO2018039084A1

公开(公告)日：2018-03-01

申请号：PCT/US2017/047692

申请日：2017-08-19

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  BRIGHAM AND WOMEN'S HOSPITAL

Inventor： JONAS, Steven J. ,  WEISS, Paul S. ,  HOU, Xu ,  AIZENBERG, Joanna ,  KHADEMHOSSEINI, Alireza

IPC: C12N5/02 ,  C12N5/00 ,  C12M1/33 ,  C12N5/071

CPC classification number: C12M23/16 ,  C12M23/20 ,  C12M23/58 ,  C12M29/00 ,  C12M35/04 ,  C12N15/907 ,  C12N2310/20

Abstract: A microfluidic device for processing cells for the intracellular delivery of molecules or other cargo includes a plurality of microchannels disposed in a substrate or chip and fluidically coupled to an inlet configured to receive a solution containing the cells and the molecules or other cargo to be delivered intracellularly to the cells. Each of the plurality of microchannels has one or more constriction regions therein, wherein the constriction regions comprise an omniphobic, superhydrophilic, or superhydrophobic surface. In some embodiments, multiple microfluidic devices operating in parallel are used to process large numbers of cells. The device and method has particularly applicability to delivering gene-editing molecules intracellularly to cells.

Abstract translation: 用于处理用于细胞内递送分子或其他货物的细胞的微流体装置包括设置在基底或芯片中并流体耦合到入口的多个微通道，所述入口构造成接收含有细胞和 分子或其他货物细胞内递送至细胞。 多个微通道中的每一个都具有一个或多个收缩区域，其中收缩区域包括全憎性，超亲水或超疏水表面。 在一些实施例中，并行操作的多个微流体装置用于处理大量的细胞。 该装置和方法特别适用于将基因编辑分子细胞内递送至细胞。

公开(公告)号：WO2018002322A2

公开(公告)日：2018-01-04

申请号：PCT/EP2017/066313

申请日：2017-06-30

Applicant: FREIE UNIVERSITÄT BERLIN

Inventor： HAAG, Rainer ,  WEINHART, Marie ,  WEI, Qiang ,  YU, Leixiao

IPC: C08G65/00 ,  A61L27/00 ,  C08G83/00 ,  C08J7/00 ,  C12M1/22 ,  C09D201/00

CPC classification number: C09D171/02 ,  C08G65/00 ,  C08G65/22 ,  C08G65/3348 ,  C08G83/00 ,  C08G2150/00 ,  C08G2650/10 ,  C08G2650/54 ,  C09D5/00 ,  C09D201/00 ,  C09D201/02 ,  C09D201/025 ,  C09D201/06 ,  C12M23/20

Abstract: The invention relates to an amphiphilic block copolymer comprising a first block consisting of a polymeric hydrophilic domain, wherein the polymeric hydrophilic domain consists of a polyglycerol; an optional second block consisting of a hydrophobic domain and a first linker domain, wherein the second block is covalently bound to the first block via the first linker domain, wherein the hydrophobic domain is chosen from the group consisting of aromatic residues having 3 to 20 carbon atoms, aliphatic residues having 3 to 20 carbon atoms, oligo(dimethylsiloxane), and poly(dimethylsiloxane); an optional third block consisting of a catechol domain and a second linker domain, wherein the third block is covalently bound to the first block via the second linker domain, wherein the catechol domain comprises at least one catechol residue; and a forth block consisting of a crosslinking domain and a third linker domain, wherein the forth block is covalently bound to the first block via the third linker domain, wherein the crosslinking domain comprises a reactive residue enabling a crosslinking between individual molecules of the amphiphilic block copolymer, wherein the reactive residue is at least one residue chosen from the group consisting of amines, thiols, allyls, vinyls, azides, alkynes, carboxyls, anhydrides, aldehydes, and benzophenone.

Abstract translation: 本发明涉及包含由聚合物亲水性结构域组成的第一嵌段的两亲嵌段共聚物，其中聚合物亲水性结构域由聚甘油组成; 任选的第二嵌段，其由疏水结构域和第一接头结构域组成，其中第二嵌段通过第一接头结构域与第一嵌段共价结合，其中疏水结构域选自具有3-20个碳的芳族残基 具有3至20个碳原子的脂族残基，低聚（二甲基硅氧烷）和聚（二甲基硅氧烷）; 由儿茶酚结构域和第二接头结构域组成的任选的第三嵌段，其中所述第三嵌段通过所述第二接头结构域与所述第一嵌段共价结合，其中所述儿茶酚结构域包含至少一个儿茶酚残基; 和由交联结构域和第三接头结构域组成的第四嵌段，其中所述第四嵌段通过所述第三接头结构域与所述第一嵌段共价结合，其中所述交联结构域包含能够在所述两亲嵌段的单个分子之间交联的反应性残基 共聚物，其中反应性残基是选自胺，硫醇，烯丙基，乙烯基，叠氮化物，炔烃，羧基，酸酐，醛和二苯甲酮的至少一个残基。

公开(公告)号：WO2017046629A1

公开(公告)日：2017-03-23

申请号：PCT/IB2015/057070

申请日：2015-09-15

Applicant: STELLENBOSCH UNIVERSITY

Inventor： KLEINTJES, Wayne George

IPC: C12N5/071 ,  A61K35/36 ,  A61L27/60 ,  A61P17/02 ,  C12M3/00

CPC classification number: C12N5/0629 ,  A61K35/36 ,  A61L27/3813 ,  A61L27/3895 ,  A61L27/60 ,  C12M23/20 ,  C12M23/34 ,  C12N2502/115 ,  C12N2533/20

Abstract: A method of culturing autogenous cells as well as a method of treating a mammalian patient is described. The cells are cultured on a substrate material having a surface treated with a fatty acid ester so as to have a strong hydrophobic surface and autogenous plasma obtained from a blood sample of the patient is used as a growth medium in order to culture the cells. The substrate material is also used as a transfer dressing for transferring the cultured cells to the patient and as a wound cover dressing. The invention also describes a kit as well as a system for culturing autogenous cells using the culture method.

Abstract translation: 描述了培养自体细胞的方法以及治疗哺乳动物患者的方法。 将细胞在具有用脂肪酸酯处理的表面的基材上培养以具有强的疏水性表面，并且将从患者的血液样品获得的自体血浆用作生长培养基以培养细胞。 底物材料还用作转运敷料，用于将培养细胞转移至患者和作为伤口覆盖物敷料。 本发明还描述了一种试剂盒以及使用培养方法培养自体细胞的系统。

公开(公告)号：WO2017023375A1

公开(公告)日：2017-02-09

申请号：PCT/US2016/027596

申请日：2016-04-14

Applicant: LOCKHEED MARTIN CORPORATION

Inventor： SIMON, Sarah M. ,  SINSABAUGH, Steven Lloyd ,  SWETT, Jacob Louis

IPC: A61L31/14 ,  A61L31/16 ,  A61L31/02

CPC classification number: C12M23/26 ,  C12M23/20 ,  C12M23/34 ,  C12M29/04

Abstract: Two-dimensional materials, particularly graphene-based materials, having a plurality of apertures therein can be formed into enclosures for various substances and a fibrous layer can be provided on an outside and/or on an inside of the enclosure. The enclosures can be exposed to an environment, such as a biological environment (in vivo or in vitro), where the fibrous layer can promote vascular ingrowth. One or more substances within the enclosure can be released into the environment, one or more selected substances from the environment can enter the enclosure, one or more selected substances from the environment can be prevented from entering the enclosure, one or more selected substances can be retained within the enclosure, or combinations thereof. The enclosure can, for example, allow a sense-response paradigm to be realized. The enclosure can, for example, provide immunoisolation for materials, such as living cells, retained therein.

Abstract translation: 可以在其中具有多个孔的二维材料，特别是石墨烯基材料形成为各种物质的外壳，并且纤维层可以设置在外壳的外部和/或内部。 外壳可以暴露于诸如生物环境（体内或体外）的环境中，其中纤维层可以促进血管向内生长。 外壳内的一种或多种物质可以释放到环境中，来自环境的一种或多种选择的物质可​​以进入外壳，可以防止来自环境的一种或多种选择的物质进入外壳，一种或多种选择的物质可​​以是 保持在外壳内，或其组合。 例如，外壳可以允许实现感应响应范例。 外壳可以例如为保留在其中的材料（例如活细胞）提供免疫隔离。

公开(公告)号：WO2017019799A1

公开(公告)日：2017-02-02

申请号：PCT/US2016/044321

申请日：2016-07-27

Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

Inventor： HUH, Dongeun ,  MONDRINOS, Mark ,  BLUNDELL, Cassidy ,  SEO, Jeongyun

IPC: A61L27/22 ,  C12M3/00 ,  G01N33/50

CPC classification number: C12M23/20 ,  A61L27/18 ,  A61L27/3804 ,  A61L27/50 ,  A61L27/52 ,  C12M21/08 ,  C12M25/14 ,  C08L83/04

Abstract: The presently disclosed subject matter provides an approach to address the needs for microscale control in shaping the spacial geometry and microarchitecture of 3D collagen hydrogels. For example, the disclosed subject matter provides for compositions, methods, and systems employing N-sulfosuccinimidyl-6-(4'-azido-2'- nitro-phenylamino)hexanoate ("sulfo-SANPAH"), to prevent detachment of the hydrogel from the anchoring substrate due to cell-mediated contraction.

Abstract translation: 目前公开的主题提供了一种方法，以解决在形成3D胶原水凝胶的空间几何形状和微结构的微观尺度控制方面的需要。 例如，所公开的主题提供了使用N-磺基琥珀酰亚胺基-6-（4'-叠氮基-2'-硝基 - 苯基氨基）己酸酯（“sulfo-SANPAH”）的组合物，方法和系统，以防止水凝胶 由于细胞介导的收缩而来自锚定底物。

公开(公告)号：WO2017019778A1

公开(公告)日：2017-02-02

申请号：PCT/US2016/044282

申请日：2016-07-27

Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

Inventor： HUH, Dongeun ,  MONDRINOS, Mark

IPC: B01L99/00 ,  B81B1/00 ,  C12M3/00 ,  C12N5/00 ,  G01N33/50

CPC classification number: C12M23/20 ,  A61L27/18 ,  A61L27/3804 ,  A61L27/50 ,  A61L27/52 ,  C12M21/08 ,  C12M25/14 ,  C08L83/04

Abstract: The presently disclosed subject matter provides a biomimetic lung disease model, and methods of its production and use. In one exemplary embodiment, the biomimetic lung disease model can include a first and second microchannel with a membrane coated with airway epithelial cells disposed between the microchannels and at least one device coupled to the biomimetic model that delivers an agent to at least one microchannel. In certain embodiments, the agent is cigarette smoke.

Abstract translation: 目前公开的主题提供仿生性肺病模型及其生产和使用的方法。 在一个示例性实施方案中，仿生性肺病模型可以包括具有涂覆有气道上皮细胞的膜的第一和第二微通道，其设置在微通道与耦合到仿生模型的至少一个装置之间，所述至少一个装置将试剂递送至至少一个微通道。 在某些实施方案中，试剂是香烟烟雾。

公开(公告)号：WO2017005927A1

公开(公告)日：2017-01-12

申请号：PCT/EP2016/066366

申请日：2016-07-08

Applicant: PARIS SCIENCES ET LETTRES - QUARTIER LATIN ,  UNIVERSITÉ PIERRE ET MARIE CURIE - PARIS 6 (UPMC) ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Inventor： CHEN, Yong ,  TANG, Yadong ,  WANG, Li ,  SHI, Jian

IPC: C12M1/12 ,  C12M1/32 ,  C12M1/09 ,  C12M1/00

CPC classification number: C12M25/02 ,  C12M23/12 ,  C12M23/20 ,  C12M23/56 ,  C12N5/0619 ,  C12N5/0657 ,  C12N5/0696 ,  C12N2506/45

Abstract: The present invention relates to a cell culture device comprising a mesh comprising or made of a biocompatible polymer; and a top grid comprising or made of a biocompatible polymer, laying over the said mesh; wherein the mesh is a monolayer of cross-linked nanofibers and has a specific surface ranging from 20% to 40%; the top grid comprises a single grid and an array of openings separated by partitions having a width; each opening of the top grid has the same geometric configuration; and the top grid comprises a border surrounding the openings, the said border having a width at least two times greater than the width of the said partitions. The present invention also relates to a method for manufacturing said cell culture device, a method of cell growth or differentiation and a cell culture system.

Abstract translation: 细胞培养装置技术领域本发明涉及包含由生物相容性聚合物制成的网状物的细胞培养装置， 和包含生物相容性聚合物制成的顶部格栅，铺设在所述网上; 其中所述网是交联纳米纤维的单层，并且具有20％至40％的比表面积; 顶部格栅包括单个格栅和由具有宽度的分隔开的开口阵列; 顶部格栅的每个开口具有相同的几何构型; 并且所述顶部格栅包括围绕所述开口的边界，所述边界具有比所述分区的宽度大至少两倍的宽度。 本发明还涉及一种制造所述细胞培养装置的方法，细胞生长或分化方法和细胞培养系统。

公开(公告)号：WO2016184600A1

公开(公告)日：2016-11-24

申请号：PCT/EP2016/057458

申请日：2016-04-05

Applicant: PLANET BIOGAS GROUP GMBH

Inventor： MEYER ZU STROHE, Jörg

IPC: B65D90/02 ,  B65D90/06 ,  C12M1/00 ,  C12M1/107

CPC classification number: C12M21/04 ,  B65D90/022 ,  B65D90/023 ,  B65D90/06 ,  C12M23/00 ,  C12M23/02 ,  C12M23/20 ,  C12M41/12

Abstract: Bei einem Großbehälter (1), mit einem Boden und einer geschlossen umlaufenden Wand (3), wobei die Wand (3) eine Vielzahl einzelner, miteinander verbundener Platten (6) aufweist, schlägt die Erfindung vor, dass die Platten (6) an ihrer ins Behälterinnere gerichteten Innenseite jeweils mit einer Folie (7) versehen sind, wobei die aneinander grenzenden, benachbarten Folien (7) durch Dichtnähte flüssigkeitsdicht miteinander verbunden sind.

Abstract translation: 在散装容器（1），具有一个底部和一个封闭的周壁（3），所述壁（3）包括多个单独的，相互连接的板（6）的，本发明提出的是在板（6）其 引导到内部的每个具有箔（7）的容器内部被提供，其中，由密封接缝的连续的，邻近膜层（7）液密的方式连接到彼此。