公开(公告)号：WO0151663A3

公开(公告)日：2002-06-13

申请号：PCT/US0101056

申请日：2001-01-10

Applicant: MAXYGEN INC ,  BASS STEVEN H ,  DAVIS S CHRISTOPHER ,  PATTEN PHILLIP A ,  TOBIN MATTHEW ,  MINSHULL JEREMY ,  WELCH MARK ,  GUSTAFSSON CLAES ,  CARR BRIAN ,  JENNE STEPHANE ,  RAILLARD SUN AI ,  CRAMERI ANDREAS ,  STEMMER WILLEM P C ,  EMIG ROBIN ,  LONGCHAMP PASCAL ,  GOLDMAN STANLEY ,  GIVER LORRAINE J ,  AFFHOLTER JOSEPH A

Inventor： BASS STEVEN H ,  DAVIS S CHRISTOPHER ,  PATTEN PHILLIP A ,  TOBIN MATTHEW ,  MINSHULL JEREMY ,  WELCH MARK ,  GUSTAFSSON CLAES ,  CARR BRIAN ,  JENNE STEPHANE ,  RAILLARD SUN AI ,  CRAMERI ANDREAS ,  STEMMER WILLEM P C ,  EMIG ROBIN ,  LONGCHAMP PASCAL ,  GOLDMAN STANLEY ,  GIVER LORRAINE J ,  AFFHOLTER JOSEPH A

IPC: G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  G01N37/00 ,  G06F19/00

CPC classification number: C12N15/1031 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/007 ,  B01J2219/00722 ,  B82Y30/00 ,  C12N15/1027 ,  C40B60/04 ,  G06F19/18 ,  Y02A90/22

Abstract: Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.

Abstract translation: 提供了用于多样化生成和筛选的集成系统和方法。 该系统使用普通液体和阵列处理组分来提供核酸多样化，转录，翻译，产物筛选和随后的多样化反应。

公开(公告)号：WO0004190A9

公开(公告)日：2000-08-10

申请号：PCT/US9915972

申请日：1999-07-15

Applicant: MAXYGEN INC ,  DEL CARDAYRE STEPHEN ,  TOBIN MATTHEW ,  STEMMER WILLEM P C ,  NESS JON E ,  MINSHULL JEREMY ,  PATTEN PHILLIP A ,  SUBRAMANIAN VENKITESWATAN ,  CASTLE LINDA A ,  KREBBER CLAUS M ,  BASS STEVE ,  ZHANG YING XIN ,  COX TONY ,  HUISMAN GJALT ,  YUAN LING ,  AFFHOLTER JOSEPH A

Inventor： DEL CARDAYRE STEPHEN ,  TOBIN MATTHEW ,  STEMMER WILLEM P C ,  NESS JON E ,  MINSHULL JEREMY ,  PATTEN PHILLIP A ,  SUBRAMANIAN VENKITESWATAN ,  CASTLE LINDA A ,  KREBBER CLAUS M ,  BASS STEVE ,  ZHANG YING-XIN ,  COX TONY ,  HUISMAN GJALT ,  YUAN LING ,  AFFHOLTER JOSEPH A

IPC: A01H5/00 ,  A01K67/027 ,  C07K14/245 ,  C12N15/09 ,  C12N15/10 ,  C12N15/82 ,  C12P17/16 ,  C12Q1/68 ,  C12N15/00

CPC classification number: C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Abstract translation: 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。

公开(公告)号：WO0164864A3

公开(公告)日：2002-03-28

申请号：PCT/US0106775

申请日：2001-02-28

Applicant: MAXYGEN INC ,  AFFHOLTER JOSEPH A ,  COX ANTHONY ,  NESS JON E ,  CARR BRIAN

Inventor： AFFHOLTER JOSEPH A ,  COX ANTHONY ,  NESS JON E ,  CARR BRIAN

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1093 ,  C12N15/1013 ,  C12N15/1027

Abstract: Methods mediated by single-stranded nucleic acid templates, including utilizing single-stranded nucleic acid templates to isolate nucleic acid fragments and to recombine nucleic acid fragments. Methods include polymerase and polymerase-free recombination of nucleic acid fragments to generate chimeric nucleic acid sequences. Integrated systems and kits are also provided.

Abstract translation: 由单链核酸模板介导的方法，包括利用单链核酸模板分离核酸片段和重组核酸片段。 方法包括核酸片段的聚合酶和无聚合酶重组以产生嵌合核酸序列。 还提供集成系统和套件。