公开(公告)号：WO2006094388A1

公开(公告)日：2006-09-14

申请号：PCT/CA2006/000321

申请日：2006-03-07

Applicant: NOVX SYSTEMS INC. ,  ALAVIE, Tino ,  BECKLEY, Keith ,  HUDSON, Andrew ,  LEONARD, Stephen, W. ,  MAASKANT, Robert ,  TALEBPOUR, Samad ,  YANG, William

Inventor： ALAVIE, Tino ,  BECKLEY, Keith ,  HUDSON, Andrew ,  LEONARD, Stephen, W. ,  MAASKANT, Robert ,  TALEBPOUR, Samad ,  YANG, William

IPC: G01N35/02 ,  G01N21/76 ,  G01N21/62 ,  G01N35/10

CPC classification number: G01N35/0092 ,  G01N21/253 ,  G01N35/028 ,  G01N35/109 ,  G01N2001/288 ,  G01N2035/00326 ,  G01N2035/0422 ,  G01N2035/0465 ,  Y10T436/11 ,  Y10T436/111666 ,  Y10T436/113332 ,  Y10T436/2575

Abstract: The present invention provides a bar-code driven, completely automated, microplate-based analyzer system for performing chemical, biochemical or biological assays. The analyzer is a modular, bench-top instrument that compactly integrates subsystems for sample dispensing, liquid handling, microplate transport, thermal incubation, vortexing, solid phase separation and optical reading. An internal processor is included for automating the instrument, and a user interface to facilitate communication with the operator via a touch-sensitive liquid-crystal display (LCD), and communicating with a remote network via multiple protocols. The analyzer includes firmware resident within the processing system and the user interface allows the operator to select pre-defined assay batch protocols and the user interface is configured in such as way so as to restrict an operator from programming the firmware.

Abstract translation: 本发明提供了用于进行化学，生物化学或生物测定的条形码驱动的，完全自动化的基于微孔板的分析仪系统。 分析仪是一种模块化的台式仪器，可紧凑集成样品分配，液体处理，微孔板输送，热孵育，涡旋，固相分离和光学读取的子系统。 包括用于使仪器自动化的内部处理器，以及用于通过触敏液晶显示器（LCD）促进与操作者通信的用户接口，以及经由多个协议与远程网络通信。 分析器包括驻留在处理系统内的固件，用户界面允许操作员选择预定义的测试批处理协议，并且用户界面被配置为以限制操作者编程固件的方式。

公开(公告)号：WO2013013304A1

公开(公告)日：2013-01-31

申请号：PCT/CA2012/000698

申请日：2012-07-25

Applicant: QVELLA CORPORATION ,  TALEBPOUR, Samad ,  KHINE, Aye Aye ,  MAASKANT, Robert ,  ALAVIE, Tino

Inventor： TALEBPOUR, Samad ,  KHINE, Aye Aye ,  MAASKANT, Robert ,  ALAVIE, Tino

IPC: C12M1/42 ,  B81B1/00 ,  B81B7/02 ,  C12M1/33 ,  C12N1/06 ,  C12N13/00 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/143 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1833 ,  B01L2400/0694 ,  C12M35/02 ,  C12M47/06 ,  C12N1/066 ,  C12N1/08 ,  C12N13/00 ,  C12Q1/686 ,  C12Q3/00

Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

Abstract translation: 提供了用于电裂解细胞并从细胞释放大分子的装置和方法。 提供了一种微流体装置，其包括具有亚毫米级厚度的平面通道，并且在其上内表面和下内表面上包括电极。 在用液体填充通道之后，使得通道包含液体内的单元，在通道电极之间施加一系列交替极性的电压脉冲，其中电压脉冲的幅度和电压脉冲的脉冲宽度对于 引起细胞的不可逆电穿孔。 该通道被配置为具有热性质，使得施加电压由于焦耳加热而导致快速升温，以从电镀单元释放大分子。 通道还可以包括用于在电处理之前捕获和集中电池的内部滤波器。

公开(公告)号：WO2011014946A1

公开(公告)日：2011-02-10

申请号：PCT/CA2010/001176

申请日：2010-07-30

Applicant: TALEBPOUR, Samad ,  KHINE, Aye Aye ,  LEONARD, Stephen W. ,  MAASKANT, Robert ,  ALAVIE, Tino

Inventor： TALEBPOUR, Samad ,  KHINE, Aye Aye ,  LEONARD, Stephen W. ,  MAASKANT, Robert ,  ALAVIE, Tino

IPC: C12M1/42 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/68 ,  G01N27/26 ,  G01N33/50 ,  G01N33/543 ,  C12N11/00 ,  C12N13/00

CPC classification number: G01N33/5438 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0809 ,  C12M23/16 ,  C12M25/00 ,  C12M25/04 ,  C12M29/10 ,  C12M35/02 ,  C12M41/38 ,  C12N1/066 ,  C12N11/14 ,  C12N13/00 ,  G01N33/5005

Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Abstract translation: 本发明提供一种微流体装置及其用于浓缩和捕获细胞的方法。 在层流下相对于样品施加脉冲非法拉第电场，这导致带电分析物的浓度和捕获。 有利地，选择脉冲定时以避免与通道内的离子屏蔽相关的问题。 通道内的至少一个电极涂有绝缘层，以防止法拉第电流在通道中流动。 在脉冲施加单极电压给电极的情况下，样品内的带电分析物经由瞬时电泳力朝向其中一个电极移动。