公开(公告)号：WO2009026566A1

公开(公告)日：2009-02-26

申请号：PCT/US2008/074107

申请日：2008-08-22

Applicant: CYNVENIO BIOSYSTEMS, LLC ,  CHANG-YEN, David, A. ,  DARABI, Jafar ,  ZHANG, Yanting ,  SOH, Hyongsok, T. ,  PAGANO, Paul

Inventor： CHANG-YEN, David, A. ,  DARABI, Jafar ,  ZHANG, Yanting ,  SOH, Hyongsok, T. ,  PAGANO, Paul

IPC: B03C1/28 ,  B03C1/01

CPC classification number: B03C1/288 ,  B03C1/01 ,  B03C2201/26

Abstract: A system for sorting and trapping magnetic target species includes a microfluidic trapping module designed to receive and then temporarily hold magnetic particles in place within the module. The magnetic particles flowing into the module are trapped there while the other sample components (non-magnetic) continuously flow through and out of the station, thereby separating and concentrating the species captured on the magnetic particles. The magnetic particles and/or their payloads may be released and separately collected at an outlet after the sample passes through the trapping module.

Abstract translation: 用于分类和捕获磁性目标物种的系统包括微流体捕获模块，其被设计成接收然后临时将磁性颗粒保持在模块内的适当位置。 流入模块的磁性颗粒被捕获在一起，而其他样品组分（非磁性）连续流过和流出站，从而分离和浓缩捕获在磁性颗粒上的物质。 在样品通过捕集模块之后，磁性颗粒和/或其有效载荷可以在出口处被释放并分开收集。

公开(公告)号：WO2007100356A3

公开(公告)日：2007-12-13

申请号：PCT/US2006038505

申请日：2006-10-02

Applicant: UNIV UTAH RES FOUND ,  GALE BRUCE K ,  MYSZKA DAVID ,  CHANG-YEN DAVID A ,  NATARAJAN SRIRAM ,  ECKMAN JOSH

Inventor： GALE BRUCE K ,  MYSZKA DAVID ,  CHANG-YEN DAVID A ,  NATARAJAN SRIRAM ,  ECKMAN JOSH

IPC: B01L3/02 ,  G01N1/10

CPC classification number: G01N33/553 ,  B01J19/0046 ,  B01L3/0293 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/021 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2400/0487 ,  C12N15/1093 ,  G01N35/1074 ,  G01N2035/00158 ,  G01N2035/1039 ,  Y10T436/2575

Abstract: Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter (10) may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells (22, 24), microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on a separate surface. Each orifice is connect to two wells via microconduits (26, 28). When the spotter contacts a surface (30), a seal is formed between th orifices (27) and the surface. The same or different substances may be flowed across each orifice. Any numb of orifices may be incorporated into a spotter. The spotter is particularly useful for depositing proteins in high concentrations on a surface, since the spotter may be placed on a surface for an extended period of time.

Abstract translation: 公开了一种用于形成微量测定器，生物芯片，生物传感器和细胞培养物的检测器装置和方法。 斑点（10）可用于将高度浓缩的蛋白质或其他材料的斑点沉积在微阵列载玻片，晶片或其它表面上。 它也可以用于在同一点上执行各种化学步骤。 点样器通过将所需物质（或其溶液）流过斑点区域直到达到表面饱和为止，增加每个斑点处物质的表面密度。 识别器可以由井板处理设备装载。 点样器使用井（22,24），微流体导管和孔口将蛋白质，其他生物分子或化学品沉积在单独表面上的一个点上。 每个孔通过微管连接到两个孔（26,28）。 当检测器接触表面（30）时，在孔口（27）和表面之间形成密封。 相同或不同的物质可能流过每个孔口。 任何麻木的孔都可以并入到一个点样器中。 鉴定器对于在表面上沉积高浓度的蛋白质是特别有用的，因为可以将定位器放置在表面上较长时间。

公开(公告)号：WO2007100356A2

公开(公告)日：2007-09-07

申请号：PCT/US2006/038505

申请日：2006-10-02

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  GALE, Bruce, K. ,  MYSZKA, David ,  CHANG-YEN, David, A. ,  NATARAJAN, Sriram ,  ECKMAN, Josh

Inventor： GALE, Bruce, K. ,  MYSZKA, David ,  CHANG-YEN, David, A. ,  NATARAJAN, Sriram ,  ECKMAN, Josh

CPC classification number: G01N33/553 ,  B01J19/0046 ,  B01L3/0293 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/021 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2400/0487 ,  C12N15/1093 ,  G01N35/1074 ,  G01N2035/00158 ,  G01N2035/1039 ,  Y10T436/2575

Abstract: Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein, cells, or other substances on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microconduits, and orifices to deliver the substances. Each orifice may be connected to two wells via microconduits. When the spotter face contacts a surface, a seal is formed between the orifices in the spotter face and the surface. The same or different substances may be flowed to each orifice and over the surface, thereby forming an array of spots on the surface. Any number of wells, microconduits, and orifices may be incorporated into a spotter.

Abstract translation: 公开了一种用于形成微量测定器，生物芯片，生物传感器和细胞培养物的检测器装置和方法。 斑点斑块可用于将高浓度的蛋白质，细胞或其他物质的斑点沉积在微阵列载玻片，晶片或其他表面上。 它也可以用于在同一点上执行各种化学步骤。 点样器通过将所需物质（或其溶液）流过斑点区域直到达到表面饱和为止，增加每个斑点处物质的表面密度。 识别器可以由井板处理设备装载。 检测员使用井，微管道和孔口来输送物质。 每个孔可以通过微管连接到两个孔。 当检查器面接触表面时，在检查器面和表面之间的孔口之间形成密封。 相同或不同的物质可以流到每个孔口和表面上，从而在表面上形成点阵列。 任何数量的井，微管和孔可以并入到一个点样器中。

公开(公告)号：WO2009129415A1

公开(公告)日：2009-10-22

申请号：PCT/US2009/040866

申请日：2009-04-16

Applicant: CYNVENIO BIOSYSTEMS, LLC ,  CHANG-YEN, David, A. ,  DARABI, Jafar ,  ZHANG, Yanting ,  PAGANO, Paul ,  GLUCK, Frederick, W.

Inventor： CHANG-YEN, David, A. ,  DARABI, Jafar ,  ZHANG, Yanting ,  PAGANO, Paul ,  GLUCK, Frederick, W.

IPC: G01N27/00

CPC classification number: G01N35/0098 ,  G01N2035/00544

Abstract: A system for sorting and trapping magnetic target species includes a microfluidic chamber designed to receive and then temporarily hold magnetic particles in place within the module. A pre-processing module may mix a sample and magnetic particles to cause certain species in the sample to be labeled. The microfluidic chamber may include a mechanism to move magnetic particles within the chamber. A post-processing module or the microfluidic chamber may be used to separate the labeled species from the magnetic particles by adding a release reagent. The magnetic particles and/or their payloads may be released and separately collected at an outlet of the chamber or the post-processing module.

Abstract translation: 用于分类和捕获磁性目标物种的系统包括微流体室，其设计成接收然后临时将磁性颗粒保持在模块内的适当位置。 预处理模块可以混合样品和磁性颗粒以使样品中的某些物质被标记。 微流体室可以包括在室内移动磁性颗粒的机构。 可以使用后处理模块或微流体室来通过添加释放试剂将标记物质与磁性颗粒分离。 磁性颗粒和/或其有效载荷可以被释放并且分别收集在腔室或后处理模块的出口处。

公开(公告)号：WO2009117611A3

公开(公告)日：2009-09-24

申请号：PCT/US2009/037714

申请日：2009-03-19

Applicant: CYNVENIO BIOSYSTEMS, LLC ,  CHANG-YEN, David, A. ,  DARABI, Jafar ,  ZHANG, Yanting ,  PAGANO, Paul

Inventor： CHANG-YEN, David, A. ,  DARABI, Jafar ,  ZHANG, Yanting ,  PAGANO, Paul

IPC: B01D35/06 ,  B03C1/30 ,  C02F1/48

Abstract: A system for sorting and trapping magnetic target species includes a microfluidic trapping chamber designed to receive and then temporarily hold magnetic particles in place within the module. An external magnetic source moves relatives to the fluid chamber as the magnetic particles flow the device in the fluidic medium. The magnetic particles flowing into the module are trapped there while the other sample components (non-magnetic) continuously flow through and out of the station, thereby separating and concentrating the species captured on the magnetic particles. The magnetic particles and/or their payloads may be released and separately collected at an outlet after the sample passes through the trapping module.

公开(公告)号：WO2009117611A2

公开(公告)日：2009-09-24

申请号：PCT/US2009037714

申请日：2009-03-19

Applicant: CYNVENIO BIOSYSTEMS LLC ,  CHANG-YEN DAVID A ,  DARABI JAFAR ,  ZHANG YANTING ,  PAGANO PAUL

Inventor： CHANG-YEN DAVID A ,  DARABI JAFAR ,  ZHANG YANTING ,  PAGANO PAUL

IPC: C12M1/00

CPC classification number: C12M47/04

Abstract: A system for sorting and trapping magnetic target species includes a microfluidic trapping chamber designed to receive and then temporarily hold magnetic particles in place within the module. An external magnetic source moves relatives to the fluid chamber as the magnetic particles flow the device in the fluidic medium. The magnetic particles flowing into the module are trapped there while the other sample components (non-magnetic) continuously flow through and out of the station, thereby separating and concentrating the species captured on the magnetic particles. The magnetic particles and/or their payloads may be released and separately collected at an outlet after the sample passes through the trapping module.

Abstract translation: 用于分类和捕获磁性目标物种的系统包括微流体捕获室，其设计成接收然后临时将磁性颗粒保持在模块内的适当位置。 当磁性颗粒将流体介质中的装置流动时，外部磁源将亲属移动到流体室。 流入模块的磁性颗粒被捕获在一起，而其他样品组分（非磁性）连续流过和流出站，从而分离和浓缩捕获在磁性颗粒上的物质。 在样品通过捕集模块之后，磁性颗粒和/或其有效载荷可以在出口处被释放并分开收集。