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    ENHANCED AMYLASE PRODUCTION BY N-TERMINAL ADDITION TO MATURE AMYLASE PROTEIN
    1.
    发明申请
    ENHANCED AMYLASE PRODUCTION BY N-TERMINAL ADDITION TO MATURE AMYLASE PROTEIN 审中-公开
    通过N末端添加成熟的淀粉酶蛋白增强了淀粉酶的生产

    公开(公告)号：WO2008118377A3

    公开(公告)日：2008-10-02

    申请号：PCT/US2008/003778

    申请日：2008-03-20

    Applicant: DANISCO US, INC., GENENCOR DIVISION ,  ENGLAND, George ,  KOLKMAN, Marc ,  VROEMEN, Casper

    Inventor： ENGLAND, George ,  KOLKMAN, Marc ,  VROEMEN, Casper

    IPC: C12N15/52

    Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amylases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.

    Abstract translation: 制备增加α-淀粉酶产生的芽孢杆菌属α-淀粉酶变体和由此产生的α-淀粉酶的方法。 可以将重组α-淀粉酶置于组合物中，并用于蔗糖加工中的洗衣洗涤剂，清洁和洗碟洗涤剂，织物退浆，淀粉液化，谷物液化，淀粉糖化，生物膜去除和淀粉水解。

    ENHANCED AMYLASE PRODUCTION BY N-TERMINAL ADDITION TO MATURE AMYLASE PROTEIN
    2.
    发明申请
    ENHANCED AMYLASE PRODUCTION BY N-TERMINAL ADDITION TO MATURE AMYLASE PROTEIN 审中-公开
    通过N-末端添加对成熟的AMYLASE蛋白的增强的AMYLASE生产

    公开(公告)号：WO2008118377A2

    公开(公告)日：2008-10-02

    申请号：PCT/US2008003778

    申请日：2008-03-20

    Applicant: DANISCO US INC GENENCOR DIV ,  ENGLAND GEORGE ,  KOLKMAN MARC ,  VROEMEN CASPER

    Inventor： ENGLAND GEORGE ,  KOLKMAN MARC ,  VROEMEN CASPER

    IPC: D06L4/40

    CPC classification number: C11D3/386 ,  C12N9/2417

    Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amylases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.

    Abstract translation: 制备增加α-淀粉酶产生的芽孢杆菌α-淀粉酶变体和由此产生的α-淀粉酶的方法。 重组α-淀粉酶可以放置在组合物中，并用于蔗糖加工中洗衣洗涤剂，清洁和餐具洗涤剂，织物退浆，淀粉液化，谷物液化，淀粉糖化，生物膜去除和淀粉水解的目的。

    BRIDGE STRUCTURES
    3.
    发明申请
    BRIDGE STRUCTURES 审中-公开
    桥结构

    公开(公告)号：WO2006111718A1

    公开(公告)日：2006-10-26

    申请号：PCT/GB2006/001383

    申请日：2006-04-18

    Applicant: ENGLAND, George, Leslie

    Inventor： ENGLAND, George, Leslie

    IPC: E01D19/02 ,  E02D31/08

    CPC classification number: E02D31/08 ,  E01D19/06

    Abstract: A bridge structure, comprising: a deck (1) for supporting a load thereon; first and second abutments (13a, b) which support respective ends of the deck and bear a compressive load from backfill (5) at the respective outer sides thereof; and at least one displacement compensation unit (21) which is configured such as to accommodate longitudinal displacement of the deck and maintain a predeterminable longitudinal force between the abutments, wherein the at least one displacement compensation unit has a load-displacement characteristic which provides for a reduced rate of change in the longitudinal force with longitudinal displacement of the deck as compared to a conventional bridge structure which incorporates no displacement compensation unit.

    Abstract translation: 一种桥梁结构，包括：用于在其上支撑载荷的甲板（1）; 第一和第二邻接部分（13a，b），其支撑甲板的相应端部并在其各自的外侧承载来自回填（5）的压缩载荷; 以及至少一个位移补偿单元（21），其被配置为适应所述甲板的纵向位移并且在所述抵接件之间保持可预定的纵向力，其中所述至少一个位移补偿单元具有负载位移特性， 与不包括位移补偿单元的常规桥式结构相比，甲板纵向位移的纵向力的变化率降低。

    PULLULANASE VARIANTS WITH INCREASED PRODUCTIVITY
    5.
    发明申请
    PULLULANASE VARIANTS WITH INCREASED PRODUCTIVITY 审中-公开
    PULLULANASE变量具有提高的生产力

    公开(公告)号：WO2008024372A2

    公开(公告)日：2008-02-28

    申请号：PCT/US2007/018523

    申请日：2007-08-21

    Applicant: DANISCO US, INC., GENENCOR DIVISION ,  ENGLAND, George ,  KOLKMAN, Marc ,  MILLER, Brian S. ,  VROEMEN, Casper

    Inventor： ENGLAND, George ,  KOLKMAN, Marc ,  MILLER, Brian S. ,  VROEMEN, Casper

    IPC: C12N9/44 ,  C12N15/12 ,  C12N5/10

    CPC classification number: C12Y302/01041 ,  C12N9/2457

    Abstract: The invention relates to novel variants of the enzymatic peptide pullulanase, the gene sequences encoding said novel peptides, expression vectors comprising those gene sequences as well as organisms expressing the novel pullulanase variants. The novel pullulanase variants of the present invention were made empirically by the use of codon-optimization procedures, selective truncation of “wild-type” molecules and through the replacement of selected amino acid residues. Furthermore, the invention relates to the use of these novel pullulanase peptides in the textile, fermentation, food and other industries.

    Abstract translation: 本发明涉及酶促支链淀粉酶的新变体，编码所述新肽的基因序列，包含那些基因序列的表达载体以及表达新型支链淀粉酶变体的生物。 通过使用密码子优化程序，选择性截短“野生型”分子和通过置换所选择的氨基酸残基，凭经验提供本发明的新型支链淀粉酶变体。 此外，本发明涉及这些新型支链淀粉酶肽在纺织，发酵，食品和其它工业中的用途。

    CONDITIONING BIOMASS FOR MICROBIAL GROWTH
    7.
    发明申请
    CONDITIONING BIOMASS FOR MICROBIAL GROWTH 审中-公开
    微生物生长调节生物量

    公开(公告)号：WO2008085356A1

    公开(公告)日：2008-07-17

    申请号：PCT/US2007/025911

    申请日：2007-12-17

    Applicant: DANISCO US, INC., GENENCOR DIVISION ,  BODIE, Elizabeth A. ,  ENGLAND, George

    Inventor： BODIE, Elizabeth A. ,  ENGLAND, George

    IPC: C12N1/22 ,  C12P21/00 ,  C12N9/02 ,  C12N9/42 ,  C12R1/885

    CPC classification number: C12N1/22 ,  C12N1/38 ,  C12N9/001 ,  C12N9/0059 ,  C12N9/0061 ,  C12N9/0071 ,  C12N9/2437

    Abstract: The present invention relates to methods for improving the yield of microbial processes that use lignocellulose biomass as a nutrient source. The methods comprise conditioning a composition comprising lignocellulose biomass with an enzyme composition that comprises a phenol oxidizing enzyme. The conditioned composition can support a higher rate of growth of microorganisms in a process. In one embodiment, a laccase composition is used to condition lignocellulose biomass derived from non-woody plants, such as corn and sugar cane. The invention also encompasses methods for culturing microorganisms that are sensitive to inhibitory compounds in lignocellulose biomass. The invention further provides methods of making a product by culturing the production microorganisms in conditioned lignocellulose biomass.

    Abstract translation: 本发明涉及提高使用木素纤维素生物质作为营养源的微生物过程产量的方法。 所述方法包括用包含酚氧化酶的酶组合物调理包含木质纤维素生物质的组合物。 经调理的组合物可以在一个过程中支持更高的微生物生长速率。 在一个实施方案中，漆酶组合物用于调节源自非木本植物如玉米和甘蔗的木质纤维素生物质。 本发明还包括培养对木质纤维素生物质中抑制性化合物敏感的微生物的方法。 本发明还提供了通过在调理的木质纤维素生物质中培养生产微生物来制备产品的方法。

    PULLULANASE VARIANTS WITH INCREASED PRODUCTIVITY
    8.
    发明申请
    PULLULANASE VARIANTS WITH INCREASED PRODUCTIVITY 审中-公开
    提高生产力的绒毛葡萄球菌变种

    公开(公告)号：WO2008024372A3

    公开(公告)日：2008-04-24

    申请号：PCT/US2007018523

    申请日：2007-08-21

    Applicant: DANISCO US INC GENENCOR DIV ,  ENGLAND GEORGE ,  KOLKMAN MARC ,  MILLER BRIAN S ,  VROEMEN CASPER

    Inventor： ENGLAND GEORGE ,  KOLKMAN MARC ,  MILLER BRIAN S ,  VROEMEN CASPER

    IPC: C12N9/44 ,  C12N5/10 ,  C12N15/12

    CPC classification number: C12Y302/01041 ,  C12N9/2457

    Abstract: The invention relates to novel variants of the enzymatic peptide pullulanase, the gene sequences encoding said novel peptides, expression vectors comprising those gene sequences as well as organisms expressing the novel pullulanase variants. The novel pullulanase variants of the present invention were made empirically by the use of codon-optimization procedures, selective truncation of "wild-type" molecules and through the replacement of selected amino acid residues. Furthermore, the invention relates to the use of these novel pullulanase peptides in the textile, fermentation, food and other industries.

    Abstract translation: 本发明涉及酶促肽支链淀粉酶的新变体，编码所述新肽的基因序列，包含那些基因序列的表达载体以及表达新型支链淀粉酶变体的生物体。 本发明的新型支链淀粉酶变体是通过使用密码子优化程序，“野生型”分子的选择性截短以及通过置换选定的氨基酸残基而凭经验制成的。 此外，本发明涉及这些新型支链淀粉酶肽在纺织，发酵，食品和其他工业中的用途。

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