公开(公告)号：WO2012007537A1

公开(公告)日：2012-01-19

申请号：PCT/EP2011/062015

申请日：2011-07-13

Applicant: DUBLIN CITY UNIVERSITY ,  LEONARD, Paul ,  DIMOV, Ivan ,  O'KENNEDY, Richard ,  FITZGERALD, Valerie

Inventor： LEONARD, Paul ,  DIMOV, Ivan ,  O'KENNEDY, Richard ,  FITZGERALD, Valerie

IPC: B01L3/00

CPC classification number: G01N33/54306 ,  B01L3/5085 ,  B01L3/50857 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/16 ,  C12Q1/6818 ,  C12Q1/6837 ,  G01N33/53 ,  G01N33/5302 ,  G01N33/545 ,  G01N33/569 ,  G01N33/68

Abstract: The present invention describes a spatial addressing technique that uses a very high-density micro-pore array for high-throughput screening of biological interactions. The therapeutic, diagnostic and drug-discovery implications of being able to identify, select and characterize specific protein-protein, protein-DNA and/or protein-carbohydrate interactions from heterogeneous populations of millions (to billions) of cells is discussed. Importantly, this technique possesses the screening and selection capacity of current display-based screening systems (i.e. millions-billions) but with greater efficiency and shorter time.

Abstract translation: 本发明描述了使用非常高密度微孔阵列进行生物相互作用的高通量筛选的空间寻址技术。 讨论了能够识别，选择和表征特定蛋白质蛋白质，蛋白质DNA和/或蛋白质 - 碳水化合物相互作用的治疗，诊断和药物发现影响，从数百万（至数十亿）细胞的异质群体。 重要的是，这种技术具有当前基于显示屏的筛选系统（即数百万亿）的筛选和选择能力，但具有更高的效率和更短的时间。