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    METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES
    2.
    发明申请
    METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES 审中-公开

    公开(公告)号：WO2002064759A3

    公开(公告)日：2002-08-22

    申请号：PCT/DK2002/000083

    申请日：2002-02-06

    Applicant: CHR. HANSEN A/S ,  HARBOE, Marianne, Kirsten

    Inventor： HARBOE, Marianne, Kirsten

    IPC: C12N9/99

    Abstract: A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.

    MICROBIALLY DERIVED RENNIN HAVING ENHANCED MILK CLOTTING ACTIVITY AND METHOD OF PRODUCING SAME
    3.
    发明申请
    MICROBIALLY DERIVED RENNIN HAVING ENHANCED MILK CLOTTING ACTIVITY AND METHOD OF PRODUCING SAME 审中-公开
    具有增强乳制品活性的微生物衍生物及其生产方法

    公开(公告)号：WO1996019582A1

    公开(公告)日：1996-06-27

    申请号：PCT/DK1995000511

    申请日：1995-12-20

    Applicant: CHR. HANSEN A/S ,  HARBOE, Marianne, Kirsten ,  KRISTENSEN, Pia, Bach

    Inventor： CHR. HANSEN A/S

    IPC: C12N15/57

    CPC classification number: C12N9/58 ,  A23C19/0326

    Abstract: Rhizomucor miehei derived aspartic proteases having enhanced milk clotting activity are obtained by deglycosylating the proteases or by producing them in microbial host species which do not glycosylate the proteases or which glycosylate them to lesser extend than Rhizomucor miehei strains naturally producing aspartic proteases in a glycosylated form. Such improved proteases are useful in cheese manufacturing.

    Abstract translation: 通过将蛋白酶去糖基化或通过在微生物宿主物种中生产来获得具有增强的凝乳活性的天冬氨酸蛋白酶，所述微生物宿主物质不将糖蛋白酶糖基化或者将它们糖基化到比天然产生糖基化形式的天冬氨酸蛋白酶的天竺葵（Rhizomucor miehei）菌株更小。 这种改良的蛋白酶可用于奶酪制造。

    A PROCESS FOR SEPARATING MILK CLOTTING ENZYMES, AND STABLE RENNET COMPOSITIONS
    5.
    发明申请
    A PROCESS FOR SEPARATING MILK CLOTTING ENZYMES, AND STABLE RENNET COMPOSITIONS 审中-公开
    分离乳制品酶的方法和稳定的网络组合物

    公开(公告)号：WO1995029999A2

    公开(公告)日：1995-11-09

    申请号：PCT/DK1995000171

    申请日：1995-04-25

    Applicant: CHR. HANSEN A/S ,  LARSEN, Robert ,  HARBOE, Marianne, Kirsten ,  LADSTEN, Pål

    Inventor： CHR. HANSEN A/S

    IPC: C12N09/64

    CPC classification number: C12N9/6481 ,  C12N9/96

    Abstract: A process of separating milk clotting enzymes in extracts of animal stomach tissue which comprises contacting a partially purified extract with an ion exchange resin under conditions where chymosin is bound to the resin and recovering the chymosin, the process optionally comprising the further step of recovering pepsin, from a pepsin-rich extract fraction using an ion exchange resin, and a liquid or powdered rennet composition containing at least 90 % of its milk clotting activity as chymosin activity, the composition comprising a chymosin stabilizing agent which is typically selected from a protein, a peptide, an amino acid or ascorbic acid.

    Abstract translation: 一种在动物胃组织提取物中分离牛奶凝固酶的方法，该方法包括在凝胶酶与树脂结合并回收凝乳酶的条件下将部分纯化的提取物与离子交换树脂接触，该方法任选地包括回收胃蛋白酶， 从含有富含胃蛋白酶的提取物级分使用离子交换树脂，以及含有至少90％的凝乳活性作为凝乳酶活性的液体或粉末凝乳酶组合物，该组合物包含凝乳酶稳定剂，其通常选自蛋白质， 肽，氨基酸或抗坏血酸。

    METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES
    8.
    发明申请
    METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES 审中-公开
    用减少酶活性提供多肽制剂的方法

    公开(公告)号：WO02064759A8

    公开(公告)日：2004-04-29

    申请号：PCT/DK0200083

    申请日：2002-02-06

    Applicant: HANSENS LAB ,  HARBOE MARIANNE KIRSTEN

    Inventor： HARBOE MARIANNE KIRSTEN

    IPC: C12N9/64 ,  C12N9/99

    CPC classification number: C12N9/6478 ,  C12N9/99

    Abstract: A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.

    Abstract translation: 一种提供多肽制剂的方法，所述多肽制剂具有减少的不期望的酶促侧活性的含量，所述方法包括使含有所需多肽（例如酶，药学活性或免疫活性多肽）的培养基在小于2的pH下经历一段时间 需要在保留所需多肽的活性的同时灭活侧活性。 该方法可用于提供乳凝固酶产品，包括基于凝乳酶或胃蛋白酶或微生物天冬氨酸蛋白酶的乳杆菌或凝血剂。 衍生自细菌种类和丝状真菌的种类。

    METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES
    9.
    发明申请
    METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES 审中-公开
    用减少酶活性提供多肽制剂的方法

    公开(公告)号：WO02064759A2

    公开(公告)日：2002-08-22

    申请号：PCT/DK0200083

    申请日：2002-02-06

    Applicant: HANSENS LAB ,  HARBOE MARIANNE KIRSTEN

    Inventor： HARBOE MARIANNE KIRSTEN

    IPC: C12N9/64 ,  C12N9/99 ,  C12N9/00

    CPC classification number: C12N9/6478 ,  C12N9/99

    Abstract: A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.

    Abstract translation: 提供多肽制剂的方法，所述多肽制剂具有减少的不期望的酶促侧活性的含量，所述方法包括使含有所需多肽（例如酶，药学活性或免疫活性多肽）的培养基在小于2的pH下经历一段时间 需要在保留所需多肽的活性的同时灭活侧活性。 该方法可用于提供乳凝固酶产物，包括基于凝乳酶或胃蛋白酶或微生物天冬氨酸蛋白酶的乳杆菌或凝血剂。 衍生自细菌种类和丝状真菌的种类。

    A METHOD OF PRODUCING CHEESE AND THE CHEESE PRODUCED BY THE METHOD
    10.
    发明申请
    A METHOD OF PRODUCING CHEESE AND THE CHEESE PRODUCED BY THE METHOD 审中-公开
    一种生产方法和方法生产的茶叶

    公开(公告)号：WO1985002980A1

    公开(公告)日：1985-07-18

    申请号：PCT/DK1984000120

    申请日：1984-12-18

    Applicant: CHR. HANSEN'S LABORATORIUM A/S ,  HOIER, Erik ,  HARBOE, Marianne, Kirsten ,  PEITERSEN, Nicolai

    Inventor： CHR. HANSEN'S LABORATORIUM A/S

    IPC: A23C19/032

    CPC classification number: A23C19/04 ,  A23C19/0285 ,  A23C19/0326

    Abstract: In a process for producing cheese from milk, an inactive milk-clotting enzyme and optionally chymosin or another suitable proteolytic enzyme is added to the milk, optionally simultaneously with or after adding a starter culture, to form a curd, at least part of the inactive enzyme is activated, and the resulting curd is converted to the ripened cheese. The milk employed is preferably concentrated milk, and the inactive milk-clotting enzyme is preferably prochymosin.

    Abstract translation: 在从牛奶生产奶酪的过程中，将无活性的凝乳酶和任选的凝乳酶或另一种合适的蛋白水解酶加入到乳中，任选地在加入起始培养物之后或之后加入起始培养物，以形成凝乳，至少部分惰性 酶被活化，所得凝乳转化成成熟的干酪。 所用的牛奶优选为浓缩乳，而无活性的凝乳酶优选为凝乳酶原。

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