公开(公告)号：WO0066773A3

公开(公告)日：2001-10-11

申请号：PCT/GB0001620

申请日：2000-04-26

Applicant: SYNGENTA LTD ,  WINDASS JOHN DAVID ,  HEANEY STEPHEN PAUL ,  RENWICK ANNABEL ,  WHITCOMBE DAVID MARK ,  LITTLE STEPHEN ,  GIBSON NEIL JAMES ,  THEAKER JANE ,  STANGER CAROLE PATRICIA

Inventor： WINDASS JOHN DAVID ,  HEANEY STEPHEN PAUL ,  RENWICK ANNABEL ,  WHITCOMBE DAVID MARK ,  LITTLE STEPHEN ,  GIBSON NEIL JAMES ,  THEAKER JANE ,  STANGER CAROLE PATRICIA

IPC: G01N33/566 ,  C07K14/37 ,  C07K14/80 ,  C12N9/02 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/569 ,  C12N15/53 ,  G06F17/30 ,  G06F19/00

CPC classification number: C12N9/0055 ,  C07K14/80 ,  C12Q1/6895 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The invention discloses methods which are particularly sensitive for detecting low frequencies of mutations in mitochondrially encoded genes, such as the cytochrome b gene, making these an especially useful and commercially important way of screening plant pathogenic fungi for the onset of fungicidal resistance wherein the resistance is due to a mutation in a mitochondrially encoded gene. The methods disclosed include single nucleotide polymorphism detection techniques especially PCR detection methods. Also disclosed are DNA sequences encoding part of the wild type and mutant cytochrome b sequences of a range of plant pathogenic fungi and the use of the sequence information to detect mutations giving rise to fungicidal resistance. Allele specific oligonucleotides and oligonucleotide probes, diagnostic primers and diagnostic kits are also disclosed.

Abstract translation: 本发明公开了对于检测线粒体编码基因如细胞色素b基因的低频突变特别敏感的方法，使得这些是筛选植物致病真菌发挥杀真菌性的特别有用和商业上重要的途径，其中抗性是 由于线粒体编码基因的突变。 所公开的方法包括单核苷酸多态性检测技术，特别是PCR检测方法。 还公开了编码一系列植物致病真菌的野生型和突变体细胞色素b序列的一部分的DNA序列，以及使用序列信息来检测产生杀真菌性的突变。 还公开了等位基因特异性寡核苷酸和寡核苷酸探针，诊断引物和诊断试剂盒。

公开(公告)号：WO1995004718A1

公开(公告)日：1995-02-16

申请号：PCT/GB1994001497

申请日：1994-07-11

Applicant: ZENECA LIMITED ,  COX, John, Michael ,  PEARSON, David, Philip, John ,  KOZAKIEWICZ, Anthony, Marian ,  YOULE, David ,  WHITTINGHAM, William, Guy ,  HEANEY, Stephen, Paul

Inventor： ZENECA LIMITED

IPC: C07D207/22

CPC classification number: C07K7/06 ,  A01N43/36 ,  C07D207/22 ,  C07K5/06139 ,  C07K5/0821 ,  C07K5/1024

Abstract: A fungicidal composition comprising a fungicidally effective amount of a compound of formula (I), wherein R is OH, optionally substituted alkoxy, optionally substituted phenoxy, optionally substituted phenylalkoxy, alkenyloxy, NR R (wherein R and R are, independently, hydrogen, optionally substituted alkyl, optionally substituted phenyl or optionally substituted phenylalkyl) or a nitrogen atom which is included in a peptide residue; or a salt thereof, and a fungicidally acceptable carrier or diluent therefor.

Abstract translation: 一种杀真菌组合物，其包含杀真菌有效量的式（I）化合物，其中R是OH，任选取代的烷氧基，任选取代的苯氧基，任选取代的苯基烷氧基，烯氧基，NR 5 R 6（其中R 5） 和R 6独立地是氢，任选取代的烷基，任选取代的苯基或任选取代的苯基烷基）或包含在肽残基中的氮原子; 或其盐，以及杀真菌可接受的载体或稀释剂。

公开(公告)号：WO1998019539A1

公开(公告)日：1998-05-14

申请号：PCT/GB1997002827

申请日：1997-10-14

Applicant: ZENECA LIMITED ,  HEANEY, Stephen, Paul ,  LAWSON, Kevin, Robert ,  FOSTER, Nicholas, Russell

Inventor： ZENECA LIMITED

IPC: A01N37/46

CPC classification number: C07C231/02 ,  A01N37/46 ,  A01N43/76 ,  A01N43/54 ,  A01N43/50 ,  A01N43/40 ,  A01N37/50 ,  A01N37/44 ,  A01N2300/00

Abstract: Fungicidal compositions comprising, as a first active ingredient, compound (A) and their use to combating fungal infections of plants. Also claimed is a process for preparing a compound of formula (I) wherein R is halogen or C1-4 alkyl.

Abstract translation: 作为第一活性成分的化合物（A）的杀真菌组合物及其对抗植物真菌感染的用途。 还要求的是制备其中R为卤素或C 1-4烷基的式（I）化合物的方法。

公开(公告)号：WO1994003061A1

公开(公告)日：1994-02-17

申请号：PCT/GB1993001471

申请日：1993-07-14

Applicant: ZENECA LIMITED ,  CROWLEY, Patrick, Jelf ,  YOULE, David ,  HEANEY, Stephen, Paul

Inventor： ZENECA LIMITED

IPC: A01N37/44

CPC classification number: A01N43/44 ,  A01N37/44

Abstract: Process for combating the pathogen Plasmopara viticola which comprises applying to plants or to their locus c &cir& _i &cir& _s &cir& _-2-aminocyclopentane-1-carboxylic acid, c &cir& _i &cir& _s &cir& _-2-aminocyclopent-3-ene-1-carboxylic acid, the (1R &cir& _, 2S &cir& _) form of either, a salt, ester or lactam thereof or a composition containing the same.

Abstract translation: 用于对抗病原体葡聚糖的方法，其包括施用于植物或其基因座，并与其连接，并且其与2-氨基环戊-1-烯-1-羧酸，c-c-氨基环戊烯-3-烯-1 - 羧酸，其盐，酯或内酰胺或其含有该组合物的组合物的（1R和C 1 -C 12，C 1 -C 10）形式。

公开(公告)号：WO1993022921A1

公开(公告)日：1993-11-25

申请号：PCT/GB1993000984

申请日：1993-05-13

Applicant: ZENECA LIMITED ,  BROWNELL, Keith, Harvey ,  GODWIN, Jeremy, Robert ,  HEANEY, Stephen, Paul ,  SKIDMORE, Anthony, Michael

Inventor： ZENECA LIMITED

IPC: A01N43/653

CPC classification number: A01N43/653 ,  A01N43/54 ,  A01N2300/00

Abstract: A fungicidal composition comprising a carrier or diluent; a first active ingredient which is a compound of formula (I), and a second active ingredient which is a compound of formula (II), wherein R is C1-4 alkyl or C3-6 cycloalkyl(C1-4)alkyl, and R is phenyl or phenyl(C1-4)alkyl; wherein the foregoing phenyl moieties are substituted with halogen; the relative amounts of the first and second active ingredients being such as to produce a synergistic effect.

Abstract translation: 包含载体或稀释剂的杀真菌组合物; 第一活性成分，其为式（I）化合物，和第二活性成分，其为式（II）化合物，其中R 1为C 1-4烷基或C 3-6环烷基（C 1-4）烷基 R 2为苯基或苯基（C 1-4）烷基; 其中前述苯基部分被卤素取代; 第一和第二活性成分的相对量是为了产生协同效应。

公开(公告)号：WO1995007022A1

公开(公告)日：1995-03-16

申请号：PCT/GB1994001751

申请日：1994-08-11

Applicant: ZENECA LIMITED ,  CROWLEY, Patrick, Jelf ,  HEANEY, Stephen, Paul ,  LAWSON, Kevin, Robert ,  YOULE, David

Inventor： ZENECA LIMITED

IPC: A01N37/44

CPC classification number: A01N43/36 ,  A01N37/44 ,  A01N37/46 ,  A01N43/08 ,  A01N43/10 ,  A01N47/18 ,  C07C229/48 ,  C07C235/54 ,  C07C237/20 ,  C07C271/24 ,  C07C2601/08 ,  C07D207/16 ,  C07D307/24 ,  Y02P20/55

Abstract: A method of combating fungi which comprises applying to a plant, to a seed of a plant or to the locus of a seed or plant a compound of formula (I), wherein X is O, S, SO, SO2, NR or CLM; A, B, D, E, L and M are, independently, hydrogen, halogen, alkyl, mono- or di-haloalkyl, benzyl (optionally substituted by halogen or haloalkyl) or hydroxy; A and B or D and E or L and M may together form a =S, =O or =CR R group; B and L or D and L may together form a bond; V is O or NR ; R and R are, independently, hydrogen or an amine protecting group; R , R and R are, independently, hydrogen, alkyl or benzyl; R and R are, independently, hydrogen, halogen, alkyl, phenyl, benzyl, alkoxy or acyloxy; or a salt thereof; provided that when X is CLM, R , R , R , A, M, D and E are all hydrogen, L and B are either both hydrogen or together form a bond, and V is O then the compound of formula (I) is in the form of a salt, but not a hydrochloride salt.

Abstract translation: 一种防治真菌的方法，其包括向植物，植物种子或种子或植物的场所施用式（I）化合物，其中X为O，S，SO，SO 2，NR 8， 或CLM; A，B，D，E，L和M独立地为氢，卤素，烷基，单 - 或二 - 卤代烷基，苄基（任选被卤素或卤代烷基）或羟基取代; A和B或D和E或L和M可以一起形成= S，= O或= CR 6 R 7组; B和L或D和L可以一起形成键; V是O或NR 4; R 1和R 2独立地是氢或胺保护基; R 3，R 4和R 8独立地是氢，烷基或苄基; R 6和R 7独立地是氢，卤素，烷基，苯基，苄基，烷氧基或酰氧基; 或其盐; 条件是当X为CLM时，R 1，R 2，R 3，A，M，D和E均为氢，L和B均为氢或一起形成键，V为O 那么式（I）的化合物是盐的形式，而不是盐酸盐。