公开(公告)号：WO2007120863A2

公开(公告)日：2007-10-25

申请号：PCT/US2007/009217

申请日：2007-04-16

Applicant: EPICENTRE TECHNOLOGIES ,  JENDRISAK, Jerome ,  MEIS, Ronald ,  DAHL, Gary

Inventor： JENDRISAK, Jerome ,  MEIS, Ronald ,  DAHL, Gary

IPC: C07H21/00 ,  C07H21/02 ,  C12N5/04 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10 ,  C12P19/34

CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

Abstract: The present invention relates to kits and methods for efficiently generating 5' capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified- nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production o 5'-capped RNA having a modified cap nucleotide and for in vitro or in vivo production o polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5'-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide- capped RNA also provides methods and kits for obtaining only type-specific or condition- specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labelin uncapped RNA comprising primary RNA transcripts or RNA having a 5 '-diphosphate with detectable dye or enzyme moieties.

Abstract translation: 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种修饰的核苷酸封端RNA分子的试剂盒和方法。 本发明用于获得这种经修饰的核苷酸封端RNA分子的新组合物。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于体外生产o具有修饰的帽核苷酸的体外或体内生产的5'-封端RNA，通过体外或体内翻译这些经修饰的核苷酸封端的RNA进行多种研究 ，治疗和商业应用。 本发明还提供用于捕获或分离未封端RNA的方法和试剂盒，其包含初级RNA转录物或具有5'-二磷酸的RNA，例如体外合成或从生物来源获得的RNA，包括与其他原核生物混合的原核mRNA 和/或真核的核酸。 用于捕获修饰的核苷酸封端RNA的方法还提供了通过在细胞中捕获的修饰的核苷酸加帽的RNA的那部分的帽依赖性减法来获得仅特定类型或条件特异性修饰的核苷酸加帽的RNA的方法和试剂盒 与另一种类型或病症细胞中的RNA相同的一种类型或病症。 本发明还提供了用于使用封端酶系统和修饰的帽子核苷酸用于标记未封端RNA的方法和试剂盒，其包含具有可检测染料或酶部分的5'-二磷酸的初级RNA转录物或RNA。

公开(公告)号：WO2006086668A3

公开(公告)日：2006-08-17

申请号：PCT/US2006/004798

申请日：2006-02-09

Applicant: EPICENTRE TECHNOLOGIES ,  JENDRISAK, Jerome ,  MEIS, Judith ,  MEIS, Ronald ,  RADEK, Agnes ,  DAHL, Gary

Inventor： JENDRISAK, Jerome ,  MEIS, Judith ,  MEIS, Ronald ,  RADEK, Agnes ,  DAHL, Gary

IPC: C12P19/34

Abstract: The present invention relates to compositions and methods employing 5'- phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5 '-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.

公开(公告)号：WO2004058989A2

公开(公告)日：2004-07-15

申请号：PCT/US2003/040912

申请日：2003-12-23

Applicant: EPICENTRE TECHNOLOGIES ,  DAHL, Gary, A. ,  JENDRISAK, Jerome, J. ,  MEIS, Judy, E. ,  VAIDYANATHAN, Ramesh

IPC: C12Q

CPC classification number: C12Q1/6862 ,  C12Q1/682 ,  C12Q1/6865 ,  C12Q2600/156 ,  C12Q2531/137 ,  C12Q2531/125 ,  C12Q2525/161 ,  C12Q2525/143

Abstract: The present invention comprises novel methods, compositions and kits comprising monopartite or bipartite target probes and an RNA polymerase to detect and quantify analytes comprising one or multiple target nucleic acid sequences, including target sequences that differ by as little as one nucleotide, or to detect and quantify non-nucleic acid analytes by detecting a target sequence tag that is joined to an analyte-binding substance. The method, called "target­dependent transcription," consists of an annealing process, a DNA ligation process, a transcription process, and a detection process. The invention also comprises novel methods, compositions and kits for amplifying RNA, including strand-displacement reverse transcription and rolling circle reverse transcription.

Abstract translation: 本发明包括新颖的方法，组合物和试剂盒，其包括单分子或双分子靶标探针和RNA聚合酶，用于检测和定量包含一个或多个靶核酸序列的分析物，包括不同于一个核苷酸的靶序列，或检测和 通过检测连接到分析物结合物质的靶序列标签来定量非核酸分析物。 称为“靶依赖性转录”的方法由退火过程，DNA连接过程，转录过程和检测过程组成。 本发明还包括用于扩增RNA的新方法，组合物和试剂盒，包括链 - 位移逆转录和滚环反转录。

公开(公告)号：WO2013102203A1

公开(公告)日：2013-07-04

申请号：PCT/US2012/072301

申请日：2012-12-31

Applicant: CELLSCRIPT, INC. ,  MEIS, Judith ,  PERSON, Anthony ,  CHIN, Cynthia ,  JENDRISAK, Jerome ,  DAHL, Gary

Inventor： MEIS, Judith ,  PERSON, Anthony ,  CHIN, Cynthia ,  JENDRISAK, Jerome ,  DAHL, Gary

IPC: C12N15/10 ,  C12N15/08 ,  C12P19/34 ,  A61K31/7105

CPC classification number: A61K48/0066 ,  A61K31/7105 ,  A61K33/06 ,  A61K38/005 ,  A61K48/0041 ,  C12N5/0619 ,  C12N5/0658 ,  C12N5/0696 ,  C12N15/1003 ,  C12N15/1096 ,  C12N15/87 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/73 ,  C12N2506/1307 ,  C12N2510/00 ,  C12P19/34 ,  C12P19/38 ,  C12P21/02 ,  C12Y301/26003 ,  A61K2300/00

Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.

Abstract translation: 本发明涉及用于制备和使用RNA组合物的组合物，试剂盒和方法，所述RNA组合物包含在哺乳动物细胞或生物体中诱导生物或生物化学作用的体外合成的ssRNA，RNA组合物重复或连续引入。 在某些实施方案中，本发明提供用于改变人或其他脊椎动物细胞的分化或表型状态的组合物和方法。 例如，本发明提供mRNA和方法，用于对表现出第二分化状态或表型的细胞，例如将人体细胞重编程为多能干细胞，对第一分化状态或表型展现细胞进行重编程。

公开(公告)号：WO2011071931A2

公开(公告)日：2011-06-16

申请号：PCT/US2010059305

申请日：2010-12-07

Applicant: KARIKO KATALIN ,  WEISSMAN DREW ,  DAHL GARY ,  PERSON ANTHONY ,  MEIS JUDITH ,  JENDRISAK JEROME

Inventor： KARIKO KATALIN ,  WEISSMAN DREW ,  DAHL GARY ,  PERSON ANTHONY ,  MEIS JUDITH ,  JENDRISAK JEROME

IPC: C12N15/113 ,  C12N5/02 ,  C12N5/071

CPC classification number: C12N5/0696 ,  A61K38/1709 ,  A61K38/1816 ,  A61K38/44 ,  A61K38/465 ,  A61K38/50 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/47 ,  C07K14/4712 ,  C07K14/505 ,  C12N9/0075 ,  C12N9/22 ,  C12N9/78 ,  C12N15/117 ,  C12N15/85 ,  C12N15/87 ,  C12N2310/17 ,  C12N2310/335 ,  C12N2320/30 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02 ,  C12Y114/13039 ,  C12Y301/04012 ,  C12Y305/04004

Abstract: The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.

Abstract translation: 本发明提供使用包含编码iPS细胞诱导因子的单链mRNA的纯化RNA制备物重编程体细胞的组合物和方法。 纯化的RNA制剂优选基本上不含RNA污染物分子，其可以：i）激活体细胞中的免疫应答，ii）降低体细胞中单链mRNA的表达，和/或iii）活性RNA传感器 在体细胞中。 在某些实施方案中，纯化的RNA制剂基本上不含部分mRNA，双链RNA，未封端的RNA分子和/或单链连续的mRNA。

公开(公告)号：WO2009135212A3

公开(公告)日：2009-11-05

申请号：PCT/US2009/042723

申请日：2009-05-04

Applicant: JENDRISAK, Jerome, J. ,  VAIDYANATHAN, Ramesh ,  DAHL, Gary ,  EPICENTRE TECHNOLOGIES CORPORATION

Inventor： JENDRISAK, Jerome, J. ,  VAIDYANATHAN, Ramesh ,  DAHL, Gary

IPC: C12N15/10 ,  C12N15/11 ,  C07H21/02 ,  C12Q1/68

Abstract: The present invention provides novel compositions, kits and methods employing RNA 5' polyphosphatases, RNA 5' monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5' ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5' ends. The 5'tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

公开(公告)号：WO2007120863A3

公开(公告)日：2008-10-30

申请号：PCT/US2007009217

申请日：2007-04-16

Applicant: EPICT TECHNOLOGIES ,  JENDRISAK JEROME ,  MEIS RONALD ,  DAHL GARY

Inventor： JENDRISAK JEROME ,  MEIS RONALD ,  DAHL GARY

IPC: C12N15/11 ,  C12N15/113 ,  C12N15/85 ,  C12P19/34

CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

Abstract: The present invention relates to kits and methods for efficiently generating 5' capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified- nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production o 5'-capped RNA having a modified cap nucleotide and for in vitro or in vivo production o polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5'-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide- capped RNA also provides methods and kits for obtaining only type-specific or condition- specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labelin uncapped RNA comprising primary RNA transcripts or RNA having a 5 '-diphosphate with detectable dye or enzyme moieties.

Abstract translation: 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种修饰的核苷酸封端RNA分子的试剂盒和方法。 本发明用于获得这种经修饰的核苷酸封端RNA分子的新组合物。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于体外生产o具有修饰的帽核苷酸的体外或体内生产的5'-封端RNA，通过体外或体内翻译这些经修饰的核苷酸封端的RNA进行多种研究 ，治疗和商业应用。 本发明还提供用于捕获或分离未封端RNA的方法和试剂盒，其包含初级RNA转录物或具有5'-二磷酸的RNA，例如体外合成或从生物来源获得的RNA，包括与其他原核生物混合的原核mRNA 和/或真核的核酸。 用于捕获修饰的核苷酸封端RNA的方法还提供了通过在细胞中捕获的修饰的核苷酸加帽的RNA的那部分的帽依赖性减法来获得仅特定类型或条件特异性修饰的核苷酸加帽的RNA的方法和试剂盒 与另一种类型或病症细胞中的RNA相同的一种类型或病症。 本发明还提供了用于使用封端酶系统和修饰的帽子核苷酸用于标记未封端RNA的方法和试剂盒，其包含具有可检测染料或酶部分的5'-二磷酸的初级RNA转录物或RNA。

公开(公告)号：WO2004059289A3

公开(公告)日：2006-03-02

申请号：PCT/US0341046

申请日：2003-12-23

Applicant: EPICT TECHNOLOGIES ,  DAHL GARY A ,  JENDRISAK JEROME J ,  DAVYDOVA ELENA ,  ROTHMAN DENES LUCIA ,  GERDES SVETLANA

IPC: C12P19/34 ,  C12Q20060101 ,  C12Q1/00 ,  C12Q1/68 ,  G01N20060101 ,  C07H21/02 ,  C07H21/04 ,  C12N9/12

CPC classification number: C12Q1/6862 ,  C12Q1/682 ,  C12Q1/6865 ,  C12Q2600/156 ,  C12Q2531/137 ,  C12Q2531/125 ,  C12Q2525/161 ,  C12Q2525/143

Abstract: The present invention comprises novel methods, compositions and kits that use N4 vRNAP deletion mutants to detect and quantify analytes comprising one or multiple target nucleic acid sequences, including target sequences that differ by as little as one nucleotide or non-nucleic acid analytes, by detecting a target sequence tag that is joined to an analyte-binding substance. The method consists of an annealing process, a DNA ligation process, an optional DNA polymerase extension process, a transcription process, and, optionally, a detection process.

Abstract translation: 本发明包括使用N4vRNAP缺失突变体检测和定量分析物的新方法，组合物和试剂盒，所述分析物包含一个或多个靶核酸序列，包括不同于一个核苷酸或非核酸分析物的靶序列，通过检测 与分析物结合物质连接的靶序列标签。 该方法由退火过程，DNA连接过程，可选的DNA聚合酶延伸过程，转录过程和任选的检测过程组成。

公开(公告)号：WO2011071931A3

公开(公告)日：2011-06-16

申请号：PCT/US2010/059305

申请日：2010-12-07

Applicant: KARIKO, Katalin ,  WEISSMAN, Drew ,  DAHL, Gary ,  PERSON, Anthony ,  MEIS, Judith ,  JENDRISAK, Jerome

Inventor： KARIKO, Katalin ,  WEISSMAN, Drew ,  DAHL, Gary ,  PERSON, Anthony ,  MEIS, Judith ,  JENDRISAK, Jerome

IPC: C12N15/113 ,  C12N5/071 ,  C12N5/02

Abstract: The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.

公开(公告)号：WO2010094040A1

公开(公告)日：2010-08-19

申请号：PCT/US2010/024319

申请日：2010-02-16

Applicant: EPICENTRE TECHNOLOGIES CORPORATION ,  JENDRISAK, Jerome ,  DECKER, Joanne ,  DAHL, Gary

Inventor： JENDRISAK, Jerome ,  DECKER, Joanne ,  DAHL, Gary

IPC: C12Q1/68 ,  C12P19/34 ,  A61K31/7105 ,  C07H21/04

CPC classification number: C12P19/34 ,  C12N9/93 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/307 ,  C12Q2527/137

Abstract: The invention provides ligation reaction mixtures, methods, and kits for improved template-independent intramolecular ligation (circularization) of linear ssDNA, including denatured gDNA fragments or first-strand cDNA made by reverse transcription of RNA, using, for example, a thermostable RNA ligase. The circular ssDNA molecules obtained using the improved ligation reaction mixtures and methods can be used, for example, as templates: for amplification by inverse PCR, rolling circle replication, transcription, or for massively parallel DNA sequencing. Applications include, for example: gene expression analysis by qPCR or using microarrays; analysis of gDNA copy number variation; and detection or quantification of specific nucleic acid sequences for research, screening, medical diagnostics, theranostics, personalized medical treatment or breeding, for purposes such as human or animal medicine, forensics, or agriculture.

Abstract translation: 本发明提供了用于改进线性ssDNA的模板无关分子内连接（环化）的连接反应混合物，方法和试剂盒，其包括使用例如热稳定的RNA连接酶，通过逆转录RNA制备的变性gDNA片段或第一链cDNA 。 使用改进的连接反应混合物和方法获得的环状ssDNA分子可用于例如模板：用于通过反向PCR扩增，滚环复制，转录或大规模并行DNA测序。 应用包括例如：通过qPCR进行基因表达分析或使用微阵列; 分析gDNA拷贝数变异; 检测或定量用于研究，筛选，医学诊断，治疗，个性化医疗或育种的特定核酸序列，用于人或动物医学，法医学或农业学等。