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1.发明申请METHOD FOR PRODUCING L-AMINO ACID USING A BACTERIUM WITH AN OPTIMIZED LEVEL OF GENE EXPRESSION 审中-公开使用具有优化基因表达水平的细菌生产L-氨基酸的方法
公开(公告)号：WO2004080386A2
公开(公告)日：2004-09-23
申请号：PCT/JP2004/003377
申请日：2004-03-12
Applicant: AJINOMOTO CO., INC. , KATASHKINA, Joanna Yosifovna , LUNTS, Maria Grigorievna , DOROSHENKO, Vera Georgievna , FOMINA, Svetlana Aleksandrovna , SKOROKHODOVA, Aleksandra Yurievna , IVANOVSKAYA, Lirina Valerievna , MASHKO, Sergei Vladimirovich
Inventor： KATASHKINA, Joanna Yosifovna , LUNTS, Maria Grigorievna , DOROSHENKO, Vera Georgievna , FOMINA, Svetlana Aleksandrovna , SKOROKHODOVA, Aleksandra Yurievna , IVANOVSKAYA, Lirina Valerievna , MASHKO, Sergei Vladimirovich
IPC: A61K
Abstract: There is provided a method for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influence on the distribution of carbon flow, such as sucAB genes, comprising the steps of introducing into chromosome of the bacterium a set of in vitro constructed DNA fragments containing regulatory element for the gene expression instead of the native element of regulatory region of the gene and selecting the colonies with increased L-amino acid productivity. Also there is provided a method for producing L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with optimized level of expression of the sucAB gene.
Abstract translation: 提供了一种获得属于埃希氏杆菌属的L-氨基酸或产生核酸的细菌的方法，其具有影响碳流分布的基因表达的最佳水平，例如sucAB基因，包括以下步骤： 将细菌的染色体引入到体外构建的含有基因表达调节元件的DNA片段，而不是基因调节区的天然元件，并选择具有增加的L-氨基酸生产力的菌落。 还提供了使用具有优化的sucAB基因表达水平的细菌生产L-氨基酸的方法，例如L-谷氨酸，L-脯氨酸，L-精氨酸，L-谷氨酰胺，L-亮氨酸。
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2.发明申请RSF1010 DERIVATIVE MOB-DEFICIENT PLASMID CONTAINING NO ANTIBIOTIC RESISTANCE GENE, BACTERIUM COMPRISING THE PLASMID AND METHOD FOR PRODUCING USEFUL METABOLITES 审中-公开RSF1010含有抗生素抗性基因的衍生物MOB缺乏型PLASMID，包含PLASMID的细菌和生产有用代谢物的方法
公开(公告)号：WO2006001514A1
公开(公告)日：2006-01-05
申请号：PCT/JP2005/012159
申请日：2005-06-24
Applicant: AJINOMOTO CO., INC. , KATASHKINA, Joanna, Yosifovna , SKOROKHODOVA, Aleksandra, Yurievna , ZIMENKOV, Danila, Vadimovich , GULEVICH, Andrey, Yurievich , ERRAIS, Lopes, Lubov , BIRYUKOVA, Irina, Vladimirovna , MIRONOV, Aleksandr, Sergeevich , MASHKO, Sergei, Vladimirovich
Inventor： KATASHKINA, Joanna, Yosifovna , SKOROKHODOVA, Aleksandra, Yurievna , ZIMENKOV, Danila, Vadimovich , GULEVICH, Andrey, Yurievich , ERRAIS, Lopes, Lubov , BIRYUKOVA, Irina, Vladimirovna , MIRONOV, Aleksandr, Sergeevich , MASHKO, Sergei, Vladimirovich
IPC: C12N15/68
Abstract: A Mob- plasmid having a RSF1010 replicon, comprising a gene coding for Rep protein and said plasmid has been modified to inactivate gene related to mobilization ability. The present invention also describes a bacterium having an ability to produce useful metabolites, comprising the plasmid and said bacterium lack active thymidylate synthase coded by thyA gene and thymidine kinase coded by tdk gene, and a method for producing useful metabolites, such as native or recombinant proteins, enzymes, L-amino acids, nucleosides and nucleotides, organic acid, vitamins, using the bacterium.
Abstract translation: 具有RSF1010复制子的Mob-质粒，其包含编码Rep蛋白的基因和所述质粒已被修饰以失活与动员能力相关的基因。 本发明还描述了具有产生有用代谢物的能力的细菌，其包含质粒和缺乏由thyA基因编码的活性胸苷酸合酶和由tdk基因编码的胸苷激酶的细菌，以及生产有用代谢物的方法，例如天然或重组 蛋白质，酶，L-氨基酸，核苷和核苷酸，有机酸，维生素，使用细菌。
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3.发明申请OPTIMIZATION OF BACTERIAL SUCAB EXPRESSION BY PROMOTER MUTAGENESIS FOR EFFICIENT L-GLUTAMIC ACID PRODUCTION 审中-公开通过促进剂促进高效L-谷氨酸生产的细菌SUCAB表达的优化
公开(公告)号：WO2004080386A3
公开(公告)日：2004-12-02
申请号：PCT/JP2004003377
申请日：2004-03-12
Applicant: AJINOMOTO KK , KATASHKINA JOANNA YOSIFOVNA , LUNTS MARIA GRIGORIEVNA , DOROSHENKO VERA GEORGIEVNA , FOMINA SVETLANA ALEKSANDROVNA , SKOROKHODOVA ALEKSANDRA YURIEV , IVANOVSKAYA LIRINA VALERIEVNA , MASHKO SERGEI VLADIMIROVICH
Inventor： KATASHKINA JOANNA YOSIFOVNA , LUNTS MARIA GRIGORIEVNA , DOROSHENKO VERA GEORGIEVNA , FOMINA SVETLANA ALEKSANDROVNA , SKOROKHODOVA ALEKSANDRA YURIEV , IVANOVSKAYA LIRINA VALERIEVNA , MASHKO SERGEI VLADIMIROVICH
IPC: A61K20060101 , C12N1/21 , C12N9/02 , C12N15/10 , C12N15/11 , C12N15/53 , C12N15/90 , C12P13/04 , C12P13/06 , C12P13/10 , C12P13/14 , C12P13/24
Abstract: There is provided a method for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influence on the distribution of carbon flow, such as sucAB genes, comprising the steps of introducing into chromosome of the bacterium a set of in vitro constructed DNA fragments containing regulatory element for the gene expression instead of the native element of regulatory region of the gene and selecting the colonies with increased L-amino acid productivity. Also there is provided a method for producing L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with optimized level of expression of the sucAB gene.
Abstract translation: 提供了一种获得属于埃希氏杆菌属的L-氨基酸或产生核酸的细菌的方法，其具有影响碳流分布的基因表达的最佳水平，例如sucAB基因，包括以下步骤： 将细菌的染色体引入到体外构建的含有基因表达调节元件的DNA片段，而不是基因调节区的天然元件，并选择具有增加的L-氨基酸生产力的菌落。 还提供了使用具有优化的sucAB基因表达水平的细菌生产L-氨基酸的方法，例如L-谷氨酸，L-脯氨酸，L-精氨酸，L-谷氨酰胺，L-亮氨酸。
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4.发明申请A BACTERIUM OF Enterobacteriaceae FAMILY PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES 审中-公开肠杆菌科细菌产生L-十六烷酸或L-十字母酸衍生的代谢物的生物体的方法和生产L-阿拉伯酸或L-阿拉伯酸衍生的代谢物的方法
公开(公告)号：WO2011087139A3
公开(公告)日：2011-10-06
申请号：PCT/JP2011050797
申请日：2011-01-12
Applicant: AJINOMOTO KK , KUVAEVA TATYANA MIKHAILOVNA , SMIRNOV SERGEY VASILIEVICH , IVANOVA OLGA NIKOLAEVNA , KIVERO ALEKSANDR DMITRIEVICH , KATASHKINA JOANNA YOSIFOVNA
Inventor： KUVAEVA TATYANA MIKHAILOVNA , SMIRNOV SERGEY VASILIEVICH , IVANOVA OLGA NIKOLAEVNA , KIVERO ALEKSANDR DMITRIEVICH , KATASHKINA JOANNA YOSIFOVNA
CPC classification number: C12P13/20 , C12N9/0016 , C12N9/88 , C12N9/93 , C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12Y104/01021
Abstract: The presently disclosed subject matter provides a bacterium of Enterobacteriaceae family producing L-aspartic acid or an L-aspartic acid-derived metabolite modified to have aspartate dehydrogenase and a method for producing L-aspartic acid or an L-aspartic acid-derived metabolite, such as L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine, using such bacterium.
Abstract translation: 目前公开的主题提供了一种产生L-天冬氨酸的肠杆菌科家族或修饰成天冬氨酸脱氢酶的L-天冬氨酸衍生的代谢物的细菌和L-天冬氨酸或L-天冬氨酸衍生的代谢产物的方法， 作为L-苏氨酸，L-赖氨酸，L-精氨酸，L-甲硫氨酸和L-高丝氨酸，使用这种细菌。
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Patent Agency Ranking5.发明申请A BACTERIUM OF Enterobacteriaceae FAMILY PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES 审中-公开肠杆菌科产生L-天冬氨酸或L-天冬氨酸衍生的代谢物的细菌及制备L-天冬氨酸或L-天冬氨酸衍生的代谢物的方法
公开(公告)号：WO2011087139A2
公开(公告)日：2011-07-21
申请号：PCT/JP2011/050797
申请日：2011-01-12
Applicant: AJINOMOTO CO.,INC. , KUVAEVA, Tatyana Mikhailovna , SMIRNOV, Sergey Vasilievich , IVANOVA, Olga Nikolaevna , KIVERO, Aleksandr Dmitrievich , KATASHKINA, Joanna Yosifovna
Inventor： KUVAEVA, Tatyana Mikhailovna , SMIRNOV, Sergey Vasilievich , IVANOVA, Olga Nikolaevna , KIVERO, Aleksandr Dmitrievich , KATASHKINA, Joanna Yosifovna
CPC classification number: C12P13/20 , C12N9/0016 , C12N9/88 , C12N9/93 , C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12Y104/01021
Abstract: The presently disclosed subject matter provides a bacterium of Enterobacteriaceae family producing L-aspartic acid or an L-aspartic acid-derived metabolite modified to have aspartate dehydrogenase and a method for producing L-aspartic acid or an L-aspartic acid-derived metabolite, such as L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine, using such bacterium.
Abstract translation: 本发明提供了产生L-天冬氨酸的肠杆菌科细菌或经修饰而具有天冬氨酸脱氢酶的L-天冬氨酸衍生代谢产物的细菌和产生 L-天冬氨酸或L-天冬氨酸衍生的代谢产物，如L-苏氨酸，L-赖氨酸，L-精氨酸，L-甲硫氨酸和L-高丝氨酸，使用这种细菌。
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公开(公告)号：WO2005080583A2
公开(公告)日：2005-09-01
申请号：PCT/JP2005003695
申请日：2005-02-25
Applicant: AJINOMOTO KK , ZIMENKOV DANILA VADIMOVICH , GULEVICH ANDREY YURIEVICH , SKOROKHODOVA ALEKSANDRA YURIEV , KATASHKINA JOANNA YOSIFOVNA , KIVERO ALEKSANDR DMITRIEVICH , BIRYUKOVA IRINA VLADIMIROVNA , DOROSHENKO VERA GEORGIEVNA , MASHKO SERGEI VLADIMIROVICH
Inventor： ZIMENKOV DANILA VADIMOVICH , GULEVICH ANDREY YURIEVICH , SKOROKHODOVA ALEKSANDRA YURIEV , KATASHKINA JOANNA YOSIFOVNA , KIVERO ALEKSANDR DMITRIEVICH , BIRYUKOVA IRINA VLADIMIROVNA , DOROSHENKO VERA GEORGIEVNA , MASHKO SERGEI VLADIMIROVICH
CPC classification number: C12P13/22 , C12N9/18 , C12Y301/01031
Abstract: A method for producing L-amino acids, such as L-tryptophan, L-phenylalanine, and L-tyrosine, using a bacterium of the Enterobacteriaceae family is provided. The L-amino acid productivity of said bacterium is increased by enhancing an activity of 6-phosphogluconolactonase, which is encoded by the pgl gene (ybhE ORF).
Abstract translation: 提供了使用肠杆菌科的细菌生产L-氨基酸的方法，如L-色氨酸，L-苯丙氨酸和L-酪氨酸。 所述细菌的L-氨基酸生产率通过增加由pgl基因（ybhE ORF）编码的6-磷酸葡糖淀粉酶的活性而增加。
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7.发明申请A BACTERIUM BELONGING TO THE GENUS Pantoea PRODUCING AN L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES 审中-公开与生产L-阿拉伯酸或L-阿拉伯酸衍生的代谢物的本发明的生物碱相关的细菌和生产L-阿拉伯酸或L-阿拉伯酸衍生的代谢物的方法
公开(公告)号：WO2010038905A1
公开(公告)日：2010-04-08
申请号：PCT/JP2009/067449
申请日：2009-09-30
Applicant: AJINOMOTO CO.,INC. , MOKHOVA, Olga Nikolaevna , KUVAEVA, Tatyana Mikhailovna , GOLUBEVA, Lyubov Igorevna , KOLOKOLOVA, Aleksandra Viktorovna , KATASHKINA, Joanna Yosifovna
Inventor： MOKHOVA, Olga Nikolaevna , KUVAEVA, Tatyana Mikhailovna , GOLUBEVA, Lyubov Igorevna , KOLOKOLOVA, Aleksandra Viktorovna , KATASHKINA, Joanna Yosifovna
Abstract: The present invention provides a bacterium belonging to the genus Pantoea which has been modified to have decreased activity of α-ketoglutarate dehydrogenase; decreased activity of citrate synthase; increased activity of phosphoenolpyruvate carboxylase or pyruvate carboxylase; and increased activity of glutamate dehydrogenase or glutamate synthase. This bacterium has the ability to produce L-aspartic acid or L-aspartic acid-derived metabolites, such as L-threonine, L-lysine, L-methionine and L-homoserine.The present invention also provides a method for producing L-aspartic acid or L-aspartic acid-derived metabolites using the bacterium.
Abstract translation: 本发明提供了一种已被修饰为具有降低的α-酮戊二酸脱氢酶活性的属于Pantoea属的细菌; 降低柠檬酸合酶的活性; 增加磷酸烯醇丙酮酸羧化酶或丙酮酸羧化酶的活性; 并增加谷氨酸脱氢酶或谷氨酸合酶的活性。 该细菌具有生产L-天冬氨酸或L-天冬氨酸衍生的代谢物如L-苏氨酸，L-赖氨酸，L-甲硫氨酸和L-高丝氨酸的能力。本发明还提供了L-天门冬氨酸生产方法 酸或L-天冬氨酸衍生的代谢产物。
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公开(公告)号：WO2006016705A1
公开(公告)日：2006-02-16
申请号：PCT/JP2005/014966
申请日：2005-08-10
Applicant: AJINOMOTO CO., INC. , KOZLOV, Yury, Ivanovich , CHINEN, Akito , IZUI, Hiroshi , HARA, Yoshihiko , YASUEDA, Hisashi , RYBAK, Konstantin, Vyacheslavovich , SLIVINSKAYA, Ekaterina, Aleksandrovna , KATASHKINA, Joanna, Yosifovna
Inventor： KOZLOV, Yury, Ivanovich , CHINEN, Akito , IZUI, Hiroshi , HARA, Yoshihiko , YASUEDA, Hisashi , RYBAK, Konstantin, Vyacheslavovich , SLIVINSKAYA, Ekaterina, Aleksandrovna , KATASHKINA, Joanna, Yosifovna
CPC classification number: C12P13/12 , C12P7/46 , C12P7/625 , C12P13/06 , C12P13/10 , C12P13/14 , C12P13/24
Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
Abstract translation: 本发明提供一种能够产生乙酰辅酶A，例如L-谷氨酸，L-谷氨酰胺，L-脯氨酸，L-精氨酸，L-亮氨酸，L-半胱氨酸，琥珀酸的有用代谢物的能力 和聚羟基丁酸酯，其中所述细菌被修饰，使得D-木酮糖-5-磷酸磷酸酮糖苷酶和/或果糖-6-磷酸磷酸酮醇酶的活性增强。 本发明还提供了使用该细菌生产有用代谢物的方法。
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公开(公告)号：WO2005080583A3
公开(公告)日：2005-09-01
申请号：PCT/JP2005/003695
申请日：2005-02-25
Applicant: AJINOMOTO CO., INC. , ZIMENKOV, Danila, Vadimovich , GULEVICH, Andrey, Yurievich , SKOROKHODOVA, Aleksandra, Yurievna , KATASHKINA, Joanna, Yosifovna , KIVERO, Aleksandr, Dmitrievich , BIRYUKOVA, Irina, Vladimirovna , DOROSHENKO, Vera, Georgievna , MASHKO, Sergei, Vladimirovich
Inventor： ZIMENKOV, Danila, Vadimovich , GULEVICH, Andrey, Yurievich , SKOROKHODOVA, Aleksandra, Yurievna , KATASHKINA, Joanna, Yosifovna , KIVERO, Aleksandr, Dmitrievich , BIRYUKOVA, Irina, Vladimirovna , DOROSHENKO, Vera, Georgievna , MASHKO, Sergei, Vladimirovich
IPC: C12N1/21
Abstract: A method for producing L-amino acids, such as L-tryptophan, L-phenylalanine, and L-tyrosine, using a bacterium of the Enterobacteriaceae family is provided. The L-amino acid productivity of said bacterium is increased by enhancing an activity of 6-phosphogluconolactonase, which is encoded by the pgl gene (ybhE ORF).
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