公开(公告)号：WO2004080386A2

公开(公告)日：2004-09-23

申请号：PCT/JP2004/003377

申请日：2004-03-12

Applicant: AJINOMOTO CO., INC. ,  KATASHKINA, Joanna Yosifovna ,  LUNTS, Maria Grigorievna ,  DOROSHENKO, Vera Georgievna ,  FOMINA, Svetlana Aleksandrovna ,  SKOROKHODOVA, Aleksandra Yurievna ,  IVANOVSKAYA, Lirina Valerievna ,  MASHKO, Sergei Vladimirovich

Inventor： KATASHKINA, Joanna Yosifovna ,  LUNTS, Maria Grigorievna ,  DOROSHENKO, Vera Georgievna ,  FOMINA, Svetlana Aleksandrovna ,  SKOROKHODOVA, Aleksandra Yurievna ,  IVANOVSKAYA, Lirina Valerievna ,  MASHKO, Sergei Vladimirovich

IPC: A61K

CPC classification number: C12P13/04 ,  C12N9/0008 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/24

Abstract: There is provided a method for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influence on the distribution of carbon flow, such as sucAB genes, comprising the steps of introducing into chromosome of the bacterium a set of in vitro constructed DNA fragments containing regulatory element for the gene expression instead of the native element of regulatory region of the gene and selecting the colonies with increased L-amino acid productivity. Also there is provided a method for producing L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with optimized level of expression of the sucAB gene.

Abstract translation: 提供了一种获得属于埃希氏杆菌属的L-氨基酸或产生核酸的细菌的方法，其具有影响碳流分布的基因表达的最佳水平，例如sucAB基因，包括以下步骤： 将细菌的染色体引入到体外构建的含有基因表达调节元件的DNA片段，而不是基因调节区的天然元件，并选择具有增加的L-氨基酸生产力的菌落。 还提供了使用具有优化的sucAB基因表达水平的细菌生产L-氨基酸的方法，例如L-谷氨酸，L-脯氨酸，L-精氨酸，L-谷氨酰胺，L-亮氨酸。

公开(公告)号：WO2004080386A3

公开(公告)日：2004-12-02

申请号：PCT/JP2004003377

申请日：2004-03-12

Applicant: AJINOMOTO KK ,  KATASHKINA JOANNA YOSIFOVNA ,  LUNTS MARIA GRIGORIEVNA ,  DOROSHENKO VERA GEORGIEVNA ,  FOMINA SVETLANA ALEKSANDROVNA ,  SKOROKHODOVA ALEKSANDRA YURIEV ,  IVANOVSKAYA LIRINA VALERIEVNA ,  MASHKO SERGEI VLADIMIROVICH

Inventor： KATASHKINA JOANNA YOSIFOVNA ,  LUNTS MARIA GRIGORIEVNA ,  DOROSHENKO VERA GEORGIEVNA ,  FOMINA SVETLANA ALEKSANDROVNA ,  SKOROKHODOVA ALEKSANDRA YURIEV ,  IVANOVSKAYA LIRINA VALERIEVNA ,  MASHKO SERGEI VLADIMIROVICH

IPC: A61K20060101 ,  C12N1/21 ,  C12N9/02 ,  C12N15/10 ,  C12N15/11 ,  C12N15/53 ,  C12N15/90 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/24

CPC classification number: C12P13/04 ,  C12N9/0008 ,  C12P13/06 ,  C12P13/10 ,  C12P13/14 ,  C12P13/24

Abstract: There is provided a method for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influence on the distribution of carbon flow, such as sucAB genes, comprising the steps of introducing into chromosome of the bacterium a set of in vitro constructed DNA fragments containing regulatory element for the gene expression instead of the native element of regulatory region of the gene and selecting the colonies with increased L-amino acid productivity. Also there is provided a method for producing L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with optimized level of expression of the sucAB gene.

Abstract translation: 提供了一种获得属于埃希氏杆菌属的L-氨基酸或产生核酸的细菌的方法，其具有影响碳流分布的基因表达的最佳水平，例如sucAB基因，包括以下步骤： 将细菌的染色体引入到体外构建的含有基因表达调节元件的DNA片段，而不是基因调节区的天然元件，并选择具有增加的L-氨基酸生产力的菌落。 还提供了使用具有优化的sucAB基因表达水平的细菌生产L-氨基酸的方法，例如L-谷氨酸，L-脯氨酸，L-精氨酸，L-谷氨酰胺，L-亮氨酸。

公开(公告)号：WO2006043730A1

公开(公告)日：2006-04-27

申请号：PCT/JP2005/019840

申请日：2005-10-21

Applicant: AJINOMOTO CO., INC. ,  RYBAK, Konstantin, Vyacheslavovich ,  SLIVINSKAYA, Ekaterina, Aleksandrovna ,  SAVRASOVA, Ekaterina, Alekseevna ,  AKHVERDIAN, Valeriy, Zavenovich ,  KLYACHKO, Elena, Vitalievna ,  MASHKO, Sergei, Vladimirovich ,  DOROSHENKO, Vera, Georgievna ,  AIRIKH, Larisa, Gotlibovna ,  LEONOVA, Tatyana, Viktorovba ,  GUSYATINER, Mikhail, Markovich ,  VOROSHILOVA, Elvira, Borisovna ,  KOZLOV, Yury, Ivanovich ,  HARA, Yoshihiko ,  UEDA, Takuji

Inventor： RYBAK, Konstantin, Vyacheslavovich ,  SLIVINSKAYA, Ekaterina, Aleksandrovna ,  SAVRASOVA, Ekaterina, Alekseevna ,  AKHVERDIAN, Valeriy, Zavenovich ,  KLYACHKO, Elena, Vitalievna ,  MASHKO, Sergei, Vladimirovich ,  DOROSHENKO, Vera, Georgievna ,  AIRIKH, Larisa, Gotlibovna ,  LEONOVA, Tatyana, Viktorovba ,  GUSYATINER, Mikhail, Markovich ,  VOROSHILOVA, Elvira, Borisovna ,  KOZLOV, Yury, Ivanovich ,  HARA, Yoshihiko ,  UEDA, Takuji

IPC: C07K14/245 ,  C12P13/04

CPC classification number: C12P13/08 ,  C12P13/10 ,  C12P13/14 ,  C12P13/222 ,  C12P13/24

Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

Abstract translation: 公开了使用肠杆菌科的细菌生产L-氨基酸的方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸或L-谷氨酸，其中细菌 已被修饰以增强D-木糖通透酶的活性。

公开(公告)号：WO2005080583A2

公开(公告)日：2005-09-01

申请号：PCT/JP2005003695

申请日：2005-02-25

Applicant: AJINOMOTO KK ,  ZIMENKOV DANILA VADIMOVICH ,  GULEVICH ANDREY YURIEVICH ,  SKOROKHODOVA ALEKSANDRA YURIEV ,  KATASHKINA JOANNA YOSIFOVNA ,  KIVERO ALEKSANDR DMITRIEVICH ,  BIRYUKOVA IRINA VLADIMIROVNA ,  DOROSHENKO VERA GEORGIEVNA ,  MASHKO SERGEI VLADIMIROVICH

Inventor： ZIMENKOV DANILA VADIMOVICH ,  GULEVICH ANDREY YURIEVICH ,  SKOROKHODOVA ALEKSANDRA YURIEV ,  KATASHKINA JOANNA YOSIFOVNA ,  KIVERO ALEKSANDR DMITRIEVICH ,  BIRYUKOVA IRINA VLADIMIROVNA ,  DOROSHENKO VERA GEORGIEVNA ,  MASHKO SERGEI VLADIMIROVICH

IPC: C12N9/18 ,  C12P13/22 ,  C12P13/00

CPC classification number: C12P13/22 ,  C12N9/18 ,  C12Y301/01031

Abstract: A method for producing L-amino acids, such as L-tryptophan, L-phenylalanine, and L-tyrosine, using a bacterium of the Enterobacteriaceae family is provided. The L-amino acid productivity of said bacterium is increased by enhancing an activity of 6-phosphogluconolactonase, which is encoded by the pgl gene (ybhE ORF).

Abstract translation: 提供了使用肠杆菌科的细菌生产L-氨基酸的方法，如L-色氨酸，L-苯丙氨酸和L-酪氨酸。 所述细菌的L-氨基酸生产率通过增加由pgl基因（ybhE ORF）编码的6-磷酸葡糖淀粉酶的活性而增加。

公开(公告)号：WO2007119890A1

公开(公告)日：2007-10-25

申请号：PCT/JP2007/058902

申请日：2007-04-18

Applicant: AJINOMOTO CO., INC. ,  FILIPPOV, Dmitriy, Vladimirovich ,  DOROSHENKO, Vera Georgievna ,  SKOROKHODOVA, Aleksandra Yurievna ,  VOROSHILOVA, Elvira Borisovna ,  GUSYATINER, Mikhail Markovich

Inventor： FILIPPOV, Dmitriy, Vladimirovich ,  DOROSHENKO, Vera Georgievna ,  SKOROKHODOVA, Aleksandra Yurievna ,  VOROSHILOVA, Elvira Borisovna ,  GUSYATINER, Mikhail Markovich

IPC: C12N1/20 ,  C12P13/04

CPC classification number: C12P13/04

Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea , which has been modified to attenuate expression of the sfmACDFH-fimZ cluster and/or the fimZ gene.

Abstract translation: 本发明提供一种使用肠杆菌科的细菌，特别是属于埃希氏杆菌属或泛菌属的细菌来生产L-氨基酸的方法，该方法已被修饰以减弱sfmACDFH-fimZ簇和/或fimZ基因的表达 。

公开(公告)号：WO2005080583A3

公开(公告)日：2005-09-01

申请号：PCT/JP2005/003695

申请日：2005-02-25

Applicant: AJINOMOTO CO., INC. ,  ZIMENKOV, Danila, Vadimovich ,  GULEVICH, Andrey, Yurievich ,  SKOROKHODOVA, Aleksandra, Yurievna ,  KATASHKINA, Joanna, Yosifovna ,  KIVERO, Aleksandr, Dmitrievich ,  BIRYUKOVA, Irina, Vladimirovna ,  DOROSHENKO, Vera, Georgievna ,  MASHKO, Sergei, Vladimirovich

Inventor： ZIMENKOV, Danila, Vadimovich ,  GULEVICH, Andrey, Yurievich ,  SKOROKHODOVA, Aleksandra, Yurievna ,  KATASHKINA, Joanna, Yosifovna ,  KIVERO, Aleksandr, Dmitrievich ,  BIRYUKOVA, Irina, Vladimirovna ,  DOROSHENKO, Vera, Georgievna ,  MASHKO, Sergei, Vladimirovich

IPC: C12N1/21

Abstract: A method for producing L-amino acids, such as L-tryptophan, L-phenylalanine, and L-tyrosine, using a bacterium of the Enterobacteriaceae family is provided. The L-amino acid productivity of said bacterium is increased by enhancing an activity of 6-phosphogluconolactonase, which is encoded by the pgl gene (ybhE ORF).