公开(公告)号：WO2016032352A1

公开(公告)日：2016-03-03

申请号：PCT/RO2015/000018

申请日：2015-07-14

Applicant: ROMVAC COMPANY SA

Inventor： PATRASCU, lonel Victor ,  CHIURCIU, Viorica ,  CHIURCIU, Constantin ,  OPORANU, Mariana ,  TOPILESCU, Georgiana

IPC: C07K1/30 ,  C07K14/79

CPC classification number: C07K14/79

Abstract: This invention refers to the method of manufacture and purification of specific ovotransferrin also called modern ovotransferrin (OTF-M) from the egg white, consisting of preparation of antigens, immunization of laying chickens with a certain antigen, purification and assessment of egg white-extracted OTF-M. The specific ovotransferrin is extracted by a two-stage precipitation with various concentrations of ammonium sulfate and citric acid, followed by dialysis and freeze-drying. This invention also refers to the composition of antigens prepared from a mixture of antibiotic-resistant strains from a single species of bacteria or of an antigen prepared from a mixture of various antibiotic-resistant species of strains, isolated from patients in Romania. This invention is susceptible for industrial use.

Abstract translation: 本发明涉及由蛋白制备特异性卵母细胞转运蛋白（也称为OTF-M）的特异性转铁蛋白的制造和纯化方法，由抗原的制备，产生某种抗原的产蛋鸡的免疫，蛋清提取的纯化和评价 OTF-M。 通过不同浓度的硫酸铵和柠檬酸两步沉淀提取特异性转铁蛋白，然后进行透析和冷冻干燥。 本发明还涉及由罗马尼亚患者分离的来自单一种细菌的抗生素抗性菌株或由各种抗生素抗性物种的混合物制备的抗原的混合物制备的抗原的组合物。 本发明易于工业使用。

公开(公告)号：WO2017065626A1

公开(公告)日：2017-04-20

申请号：PCT/RO2016/000002

申请日：2016-01-08

Applicant: ROMVAC COMPANY SA

Inventor： CONSTANTIN, Chiurciu ,  VIORICA, Chiurciu ,  SIMA, Lucica ,  MIHAI, Iuliana ,  IONEL VICTOR PATRASCU

IPC: A61K39/395 ,  C07K16/02

CPC classification number: C07K16/02 ,  A61K39/39533 ,  A61K2039/54

Abstract: This invention refers to the discovery of the way of treating psoriasis using personalized chicken immunoglobulin therapy. Chickens are immunized with an immunogen prepared from skin wound samples taken from each and every patient. This invention refers to the a particular immunogen, taken from the patient, mixed with an adjuvant and which, when administered in chickens, yields a specific immune response within an appropriate period of time so that the Ovopach eggs obtained from these chickens should be used under stringent conditions in the psoriasis treatment of the patient. The immunogen is polyspecific and contains microbial and cellular antigens found in the psoriasis wounds of the patient. The specific immune response from the immunized chickens is monitored on daily basis by extraction of immunoglobulins (Ig) from the egg yolk (Y) and qualitative and quantitative assessment by ELISA, radial immunodiffusion and Ouchterlony immunodiffusion assay. The immune response of chickens, expressed by the yolk-extracted antibodies, is a complex, multiple response against all antigenic stimuli present in the pathogenic product taken from the patient.

Abstract translation: 本发明涉及使用个性化鸡免疫球蛋白疗法治疗牛皮癣的方式的发现。 用从每位患者采集的皮肤伤口样品制备的免疫原免疫鸡。 本发明涉及取自患者的特定免疫原与佐剂混合并且当其在鸡中施用时在合适的时间段内产生特异性免疫应答，从而使得从这些鸡获得的Ovopach卵应用于 在患者的牛皮癣治疗中的严格条件。 免疫原是多特异性的并且含有在患者的牛皮癣伤口中发现的微生物和细胞抗原。 通过从蛋黄中提取免疫球蛋白（Ig）并通过ELISA，径向免疫扩散和Ouchterlony免疫扩散测定进行定性和定量评估，每日监测免疫鸡的特异性免疫应答。 由蛋黄提取的抗体表达的鸡的免疫应答是针对来自病人的致病产物中存在的所有抗原刺激的复杂的多重应答。

公开(公告)号：WO2016114678A1

公开(公告)日：2016-07-21

申请号：PCT/RO2016/000001

申请日：2016-01-08

Applicant: ROMVAC COMPANY SA

Inventor： PATRASCU, Ionel, Victor ,  CHIURCIU, Victoria ,  CHIURCIU, Constantin ,  OPORANU, Mariana ,  TOPILESCU, Georgiana ,  IULIANA, Mihai

IPC: C07K14/79

CPC classification number: C07K14/79

Abstract: This invention refers to the discovery of the modulation capacity of ovotransferrin to generate immune response against the antigens which chickens have been immunized against. Immunomodulators such as viral, bacterial and fungal antigens are used for chicken immunization. This invention describes the specific immune response of OTF-PC2 depending on the structure of the monospecific or polyspecific antigen used. The polyspecific antigen consists of a mixture of viral, bacterial and fungal antigens. Ovotransferrin PC-2 has a positive reaction against all viral, bacterial and fungal antigens which the chicken has been immunized against in the rapid and slow seroprecipitation assays, agar gel immunodiffusion assay, ELISA and bacterial growth inhibition assay PaChi 1MB. This invention is complementary to the invention registered at OSIM under No. A/00653 from 28.08.2014.

Abstract translation: 本发明涉及发现转铁蛋白调节能力以产生针对鸡免疫抗原的抗原的免疫应答。 免疫调节剂如病毒，细菌和真菌抗原用于鸡免疫。 本发明根据所使用的单特异性或多特异性抗原的结构描述OTF-PC2的特异性免疫应答。 多特异性抗原由病毒，细菌和真菌抗原的混合物组成。 卵清转铁蛋白PC-2对所有病毒，细菌和真菌抗原具有积极的反应，鸡在已经快速和缓慢的血清沉淀测定，琼脂凝胶免疫扩散测定，ELISA和细菌生长抑制测定法PaChi 1MB中已被免疫。 本发明是从OS.0.0从28.08.2014的A / 00653号注册的发明的补充。

公开(公告)号：WO2016114677A1

公开(公告)日：2016-07-21

申请号：PCT/RO2015/000019

申请日：2015-07-21

Applicant: ROMVAC COMPANY SA

Inventor： PATRASCU, Ionel, Victor ,  CHIURCIU, Viorica ,  CHIURCIU, Constantin ,  MIHAI, Iiuliana ,  TOPILESCU, Georgiana

IPC: C07K16/02 ,  C07K16/08 ,  C07K16/12

CPC classification number: C07K16/02 ,  C07K16/087 ,  C07K16/1214 ,  C07K16/1217 ,  C07K16/1228 ,  C07K16/1232 ,  C07K16/1235 ,  C07K16/1242 ,  C07K16/1271 ,  C07K16/1275 ,  C07K16/1282 ,  C07K2317/23

Abstract: This invention refers to the manufacturing method of the hyperimmune egg-PC2 consisting of preparation of antigens and immunization of laying chickens with a mixture of antigens prepared from human- and animal-pathogenic germs. This invention refers to the composition of antigens prepared from a mixture of antibiotic-resistant strains from a single bacterial species and from a mixture of various species of bacteria, viruses and fungi, all representing a stimulus administered in each chicken.

Abstract translation: 本发明涉及由抗原制备和由人和动物致病菌制备的抗原混合物产蛋鸡免疫的超免疫卵PC2的制造方法。 本发明涉及由来自单一菌种的抗生素抗性菌株和各种细菌，病毒和真菌的混合物的混合物制备的抗原的组合物，所有这些都表示在每只鸡中施用的刺激物。