公开(公告)号：WO2010009147A1

公开(公告)日：2010-01-21

申请号：PCT/US2009/050566

申请日：2009-07-14

Applicant: PRECISION BIOSCIENCES, INC. ,  JANTZ, Derek ,  SMITH, James, Jefferson

Inventor： JANTZ, Derek ,  SMITH, James, Jefferson

IPC: C12N9/22 ,  C12N5/10

CPC classification number: C12Q1/6811 ,  C12N9/22 ,  G06Q99/00

Abstract: Methods of cleaving double-stranded DNA that can be recognized and cleaved by a rationally-designed, I-Crel-derived meganuclease are provided. Also provided are recombinant nucleic acids, cells, and organisms containing such recombinant nucleic acids, as well as cells and organisms produced using such meganucleases. Also provided are methods of conducting a custom-designed, I-Crel-derived meganuclease business.

Abstract translation: 提供了可以通过合理设计的I-CreI衍生的大范围核酸酶识别和切割的双链DNA的切割方法。 还提供了含有这种重组核酸的重组核酸，细胞和生物，以及使用这种大范围核酸酶产生的细胞和生物体。 还提供了进行定制设计的I-Crel衍生的大范围核酸酶业务的方法。

公开(公告)号：WO2009059195A3

公开(公告)日：2009-05-07

申请号：PCT/US2008/082072

申请日：2008-10-31

Applicant: PRECISION BIOSCIENCES ,  SMITH, James, Jefferson ,  JANTZ, Derek

Inventor： SMITH, James, Jefferson ,  JANTZ, Derek

IPC: C12N9/22

Abstract: Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non- palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases.

公开(公告)号：WO2007047859A3

公开(公告)日：2007-11-15

申请号：PCT/US2006040919

申请日：2006-10-18

Applicant: PREC BIOSCIENCES ,  HELLINGA HOMME W ,  SMITH JAMES JEFFERSON ,  JANTZ DEREK

Inventor： HELLINGA HOMME W ,  SMITH JAMES JEFFERSON ,  JANTZ DEREK

IPC: C12N9/22 ,  C12N15/90

CPC classification number: C12N9/22 ,  A61K38/465 ,  A61K48/00 ,  C12N15/8213 ,  C12N15/902 ,  C12N15/905 ,  C12N15/907

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract translation: 提供合理设计的LAGLIDADG大范围核酸酶和制备这种大范围核酸酶的方法。 此外，提供了使用大范围核酸酶产生重组细胞和生物体的方法，所述重组细胞和生物体具有插入基因组内有限数量基因座的所需DNA序列，以及基因治疗方法，用于治疗病原体感染和体外 应用于诊断和研究。

公开(公告)号：WO2013066423A8

公开(公告)日：2014-07-10

申请号：PCT/US2012043082

申请日：2012-06-19

Applicant: PIONEER HI BRED INT ,  DU PONT ,  BIDNEY DENNIS L ,  CIGAN MARK ,  FALCO CARL ,  GAO HUIRONG ,  JANTZ DEREK ,  LASSNER MIKE ,  LOWE KEITH ,  LYZNIK LESZEK ALEKSANDER ,  SMITH JAMES JEFFERSON

Inventor： BIDNEY DENNIS L ,  CIGAN MARK ,  FALCO CARL ,  GAO HUIRONG ,  JANTZ DEREK ,  LASSNER MIKE ,  LOWE KEITH ,  LYZNIK LESZEK ALEKSANDER ,  SMITH JAMES JEFFERSON

IPC: C12N15/82

CPC classification number: C12N15/8289 ,  C12N5/14 ,  C12N9/22 ,  C12N15/8213 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y301/00

Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand- break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

Abstract translation: 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一种靶向修饰的雄性育性基因的核酸分子，编码作为工程化双链断裂诱导剂和表达盒的核酸内切酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。

公开(公告)号：WO2012129373A3

公开(公告)日：2013-03-14

申请号：PCT/US2012030061

申请日：2012-03-22

Applicant: PIONEER HI BRED INT ,  DU PONT ,  LASSNER MICHAEL ,  JANTZ DEREK ,  SMITH JAMES JEFFERSON ,  CIGAN MARK ,  FALCO CARL ,  GAO HUIRONG ,  LI ZHONGSEN ,  LIU ZHAN-BIN ,  SVITASHEV SERGEI

Inventor： LASSNER MICHAEL ,  JANTZ DEREK ,  SMITH JAMES JEFFERSON ,  CIGAN MARK ,  FALCO CARL ,  GAO HUIRONG ,  LI ZHONGSEN ,  LIU ZHAN-BIN ,  SVITASHEV SERGEI

IPC: C12N15/82 ,  A01H1/06 ,  A01H5/10 ,  C12N15/55

CPC classification number: C12N15/8202 ,  A01H1/06 ,  C12N9/22 ,  C12N15/8213

Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

Abstract translation: 公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。 所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。 还公开了植物中的复杂的转基因性状基因座。 复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。 还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

公开(公告)号：WO2012167192A3

公开(公告)日：2012-12-06

申请号：PCT/US2012/040599

申请日：2012-06-01

Applicant: PRECISION BIOSCIENCES, INC. ,  JANTZ, Derek ,  SMITH, James, Jefferson ,  NICHOLSON, Michael, G.

Inventor： JANTZ, Derek ,  SMITH, James, Jefferson ,  NICHOLSON, Michael, G.

IPC: C12N15/66

Abstract: Methods of inserting genes into defined locations in the chromosomal DNA of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest. The invention also relates to meganucleases, vectors and engineered cell lines necessary for performing the methods, to cell lines resulting from the application of the methods, and use of the cell lines to produce protein products of interest.

公开(公告)号：WO2012167192A2

公开(公告)日：2012-12-06

申请号：PCT/US2012040599

申请日：2012-06-01

Applicant: PREC BIOSCIENCES INC ,  JANTZ DEREK ,  SMITH JAMES JEFFERSON ,  NICHOLSON MICHAEL G

Inventor： JANTZ DEREK ,  SMITH JAMES JEFFERSON ,  NICHOLSON MICHAEL G

IPC: C12Q1/68 ,  C12N15/85

CPC classification number: C12N15/907 ,  C12N9/003 ,  C12N9/22 ,  C12N9/93 ,  C12Y105/01003 ,  C12Y301/04 ,  C12Y603/01002

Abstract: Methods of inserting genes into defined locations in the chromosomal DNA of cultured mammalian cell lines which are subject to gene amplification are disclosed. In particular, sequences of interest (e.g., genes encoding biotherapeutic proteins) are inserted proximal to selectable genes in amplifiable loci, and the transformed cells are subjected to selection to induce co-amplification of the selectable gene and the sequence of interest. The invention also relates to meganucleases, vectors and engineered cell lines necessary for performing the methods, to cell lines resulting from the application of the methods, and use of the cell lines to produce protein products of interest.

Abstract translation: 公开了将基因插入受基因扩增的培养的哺乳动物细胞系的染色体DNA中的限定位置的方法。 特别地，将感兴趣的序列（例如，编码生物治疗性蛋白的基因）插入到可扩增位点中的可选择基因的近端，并且对转化的细胞进行选择以诱导可选择基因和感兴趣的序列的共扩增。 本发明还涉及实施方法所需的大范围核酸酶，载体和工程细胞系，应用该方法产生的细胞系，以及细胞系用于产生目的蛋白质产物的用途。

公开(公告)号：WO2012129373A2

公开(公告)日：2012-09-27

申请号：PCT/US2012/030061

申请日：2012-03-22

Applicant: PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DU PONT DE NEMOURS AND COMPANY ,  LASSNER, Michael ,  JANTZ, Derek ,  SMITH, James, Jefferson ,  CIGAN, Mark ,  FALCO, Carl ,  GAO, Huirong ,  LI, Zhongsen ,  LIU, Zhan-bin ,  SVITASHEV, Sergei

Inventor： LASSNER, Michael ,  JANTZ, Derek ,  SMITH, James, Jefferson ,  CIGAN, Mark ,  FALCO, Carl ,  GAO, Huirong ,  LI, Zhongsen ,  LIU, Zhan-bin ,  SVITASHEV, Sergei

IPC: C12N15/82 ,  A01H1/06 ,  A01H5/10 ,  C12N15/55

CPC classification number: C12N15/8202 ,  A01H1/06 ,  C12N9/22 ,  C12N15/8213

Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

Abstract translation: 公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。 所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。 还公开了植物中的复杂的转基因性状基因座。 复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。 还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

公开(公告)号：WO2009114321A2

公开(公告)日：2009-09-17

申请号：PCT/US2009/035719

申请日：2009-03-02

Applicant: PRECISION BIOSCIENCS, INC. ,  JANTZ, Derek ,  SMITH, James, Jefferson

Inventor： JANTZ, Derek ,  SMITH, James, Jefferson

IPC: G01D13/28

CPC classification number: C12N9/22

Abstract: The invention relates to the field of molecular biology and recombinant nucleic acid technology. In particular, the invention relates to a rationally-designed, non-naturally- occurring meganuclease with altered DNA recognition sequence specificity which recognizes and cleaves a unique DNA site in the maize genome. The invention also relates to methods of producing engineered maize plants using such meganuc leases.

Abstract translation: 本发明涉及分子生物学和重组核酸技术领域。 特别地，本发明涉及具有改变的DNA识别序列特异性的合理设计的非天然存在的大范围核酸酶，其识别和切割玉米基因组中独特的DNA位点。 本发明还涉及使用这种大豆原料生产工程化玉米植物的方法。

公开(公告)号：WO2009076292A2

公开(公告)日：2009-06-18

申请号：PCT/US2008/085878

申请日：2008-12-08

Applicant: PRECISION BIOSCIENCES, INC. ,  JANTZ, Derek ,  NICHOLSON, Michael, G. ,  SMITH, James, Jefferson

Inventor： JANTZ, Derek ,  NICHOLSON, Michael, G. ,  SMITH, James, Jefferson

IPC: C12Q1/68 ,  C12N9/22

CPC classification number: C12N9/16 ,  C12N9/22

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

Abstract translation: 提供合理设计的LAGLIDADG大范围核酸酶和制备这种大范围核酸酶的方法。 此外，提供了使用大范围核酸酶产生重组细胞和生物体的方法，所述重组细胞和生物体具有插入基因组内有限数量基因座的所需DNA序列，以及基因治疗方法，用于治疗病原体感染和体外 应用于诊断和研究。