A METHOD FOR ENGINEERING STRAND-SPECIFIC, SEQUENCE-SPECIFIC, DNA-NICKING ENZYMES

    公开(公告)号:WO2003055975A3

    公开(公告)日:2003-07-10

    申请号:PCT/US2002/026273

    申请日:2002-08-16

    Abstract: Methods are provided for converting into a sequence specific strand specific and location specific DNA nicking endonuclease, a restriction endonuclease that recognizes an asymmetric DNA sequence, the endonuclease having two catalytic sites and one or more single sequence specific DNA-binding domains. In one embodiment the method requires inactivating one of the catalytic sites of the restriction endonuclease. In another embodiment, the restriction endonuclease is a dimer having a first and second subunit each comprising a sequence specific DNA binding domain, a catalytic site and a dimerization domain. The nicking endonuclease is formed from combining one subunit having an inactivated catalytic site and a second subunit having an inactivated DNA binding domain. The nicking endonuclease may be converted into a restriction endonuclease by the addition of manganese cations in the digestion buffer.

    A MANIPULATOR FOR METAL INGOTS
    3.
    发明申请
    A MANIPULATOR FOR METAL INGOTS 审中-公开
    用于金属加工的操纵器

    公开(公告)号:WO1988006498A1

    公开(公告)日:1988-09-07

    申请号:PCT/GB1988000152

    申请日:1988-03-02

    CPC classification number: B21J13/10

    Abstract: A manipulator for manipulating metal ingots comprises a wheeled carriage (3, 103) with a peel assembly (1, 101) supported on it so that the peel assembly can move relative to the carriage. The peel assembly is supported adjacent one end of the carriage by at least one piston-cylinder device (53, 152) and a plurality of elongate links (66, 69, 169, 170) are pivotally coupled to the peel assembly and to a rigid support means (65, 165, 166, 167) on the carriage away from the end.

    Abstract translation: 用于操作金属锭的操纵器包括具有支撑在其上的剥离组件(1,101)的轮式托架(3,103),使得剥离组件相对于托架移动。 剥离组件通过至少一个活塞 - 缸装置(53,152)支撑在托架的一端附近,并且多个细长连杆(66,69,169,170)可枢转地联接到剥离组件和刚性 支架装置(65,165,166,167)在托架上离开末端。

    MEDICAL IMPLANTS WITH POLYSACCHARIDE DRUG ELUTING COATINGS
    4.
    发明申请
    MEDICAL IMPLANTS WITH POLYSACCHARIDE DRUG ELUTING COATINGS 审中-公开
    具有多糖类药物涂层的医用植入物

    公开(公告)号:WO2009102787A2

    公开(公告)日:2009-08-20

    申请号:PCT/US2009033792

    申请日:2009-02-11

    Inventor: WILSON GEOFFREY

    Abstract: A medical implant can include a bioerodible metal portion and a coating overlying the bioerodible metal portion. The coating can include a therapeutic agent and a polysaccharide matrix reversibly cross-linked with polyvalent metal cations. Upon implantation of the implant within a body, the therapeutic agent is released and the bioerodible metal portion erodes to release polyvalent metal cations capable of re-cross-linking the polysaccharide matrix.

    Abstract translation: 医疗植入物可以包括生物腐蚀性金属部分和覆盖在生物腐蚀性金属部分上的涂层。 涂层可以包括治疗剂和与多价金属阳离子可逆交联的多糖基质。 植入物在体内时,治疗剂被释放,并且生物可腐蚀金属部分侵蚀以释放能够重新交联多糖基质的多价金属阳离子。

    OPTICAL BENCH FOR DIODE-PUMPED SOLID STATE LASERS IN FIELD APPLICATIONS
    5.
    发明申请
    OPTICAL BENCH FOR DIODE-PUMPED SOLID STATE LASERS IN FIELD APPLICATIONS 审中-公开
    用于现场应用的二极管泵浦固体激光器光学平台

    公开(公告)号:WO2005050797A2

    公开(公告)日:2005-06-02

    申请号:PCT/US2004/037977

    申请日:2004-11-12

    Abstract: A rugged resonator for a laser includes a laser pump housing. An X-fold resonator assembly is configured to receive and hold the laser pump housing. The resonator includes at least four mirrors, a first, a second, a third, and a fourth mirror. The first mirror is configured to receive a first incident laser beam from the first end and to reflect a first reflected beam to reflect as a third reflected beam from the third mirror. The second mirror is configured to receive a second incident laser beam from the second end and reflecting a second reflected beam to intersect the first reflected beam and to reflect as a fourth reflected beam from the fourth mirror. An optical coupler is affixed to an optics plate and configured to receive the fourth reflected beam. A high reflector is affixed to the optics plate and configured to receive the third reflected beam.

    Abstract translation: 用于激光器的坚固的谐振器包括激光泵壳体。 X折叠谐振器组件被构造成接收并保持激光泵壳体。 该谐振器包括至少四个反射镜,第一反射镜,第二反射镜,第三反射镜和第四反射镜。 第一反射镜被配置为接收来自第一端的第一入射激光束并反射第一反射光束以作为来自第三反射镜的第三反射光束反射。 第二反射镜被配置为接收来自第二端的第二入射激光束并且反射第二反射光束以与第一反射光束相交并作为来自第四反射镜的第四反射光束反射。 光耦合器被固定到光学板上并被配置为接收第四反射光束。 一个高反射镜固定在光学板上,并配置为接收第三个反射光束。

    A GOLF CLUB
    6.
    发明申请
    A GOLF CLUB 审中-公开
    高尔夫俱乐部

    公开(公告)号:WO1997045172A1

    公开(公告)日:1997-12-04

    申请号:PCT/GB1997001456

    申请日:1997-05-29

    Inventor: TIDYMAKE LIMITED

    Abstract: A golf club comprises a shaft (3) and a club head (2) which is connected to a shank portion (4) of the shaft at its heel end (31) so that it can be rotated around the axis of the shank portion. The configuration of the shank portion is such that the shaft axis and the shank axis intersect at about the bottom of the heel end of the club head so that rotating the club head around the shank axis causes the angle between the club face (30) and the shaft (3) to change.

    Abstract translation: 高尔夫球杆包括轴(3)和球杆杆头(2),其在其后端(31)处连接到轴的柄部(4),使得其可以围绕柄部的轴线旋转。 柄部分的构造使得轴线和柄轴线在球杆头的后端的底部附近相交,从而使杆头围绕柄轴线转动会使球杆面(30)和 轴(3)改变。

    METHOD OF, AND APPARATUS FOR, MANUFACTURING A COILED SHEATH FOR ELECTRICAL CORDS
    7.
    发明申请
    METHOD OF, AND APPARATUS FOR, MANUFACTURING A COILED SHEATH FOR ELECTRICAL CORDS 审中-公开
    用于制造电线的线圈的方法和装置

    公开(公告)号:WO1993015899A1

    公开(公告)日:1993-08-19

    申请号:PCT/AU1993000036

    申请日:1993-01-27

    CPC classification number: H01B13/008 B29C53/12 H02G1/085

    Abstract: A coiled sheath (10) for electrical cords is formed of polyethylene tube drawn from a supply (20). The tubular body (10) is drawn through a bath of glycerine at, e.g. 125 DEG C and a slit is formed in the tubular body (10) by a knife (22). A continuous core (24) is inserted through the slit in the tubular body (10) and the body (40) is wound around the mandrel (25) at least three times before the core (24) is stripped. The body (10) is passed around the mandrel (25), which is in a cold water bath (26) at least one more time and is then cooled in the opposite direction before being cut to predetermined lengths by a docking knife (27).

    Abstract translation: 用于电线的线圈护套(10)由从电源(20)拉出的聚乙烯管形成。 管状体(10)通过例如甘油的浴浸入。 125℃,并且通过刀(22)在管状体(10)中形成狭缝。 在芯体(24)被剥离之前,将连续的芯(24)插入穿过管状体(10)中的狭缝并且主体(40)围绕心轴(25)缠绕至少三次。 主体(10)绕心轴(25)至少再次在冷水浴(26)中通过,然后在通过对接刀(27)切割成预定长度之前沿相反方向冷却, 。

    TREATMENT OF METAL SLABS
    8.
    发明申请
    TREATMENT OF METAL SLABS 审中-公开
    金属渣的处理

    公开(公告)号:WO1990002003A1

    公开(公告)日:1990-03-08

    申请号:PCT/GB1989000986

    申请日:1989-08-24

    CPC classification number: B21B15/0035

    Abstract: A pressing machine for reducing the width of a hot metal slab has a pair of platens which are caused to engage with opposite edges of the slab to apply forces which reduce the width of the slab. This pressing action may cause buckling of the slab and to prevent, or at least reduce, the buckling, one or both of the opposite faces of the slab has a restraint provided adjacent thereto. The restraint may be in two parts connected to respective platens and arranged to allow for movement of one platen with respect to the other platen.

    Abstract translation: 用于减小热金属板坯的宽度的压制机具有一对压板,其与板坯的相对边缘接合以施加减小板坯宽度的力。 这种按压作用可能导致板坯的翘曲并且防止或至少减少弯曲,板坯的相对面中的一个或两个具有邻近设置的约束。 约束可以是连接到相应压板的两个部分,并且被布置成允许一个压板相对于另一个压板移动。

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